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Automated NGS library preparation

The NGS library preparation process is complex and has numerous steps, making automation a daunting prospect. With Opentrons robots and proven protocols, library preparation can be the easiest part of your day.

Download from protocol library

You don’t even need to program the robot to do library preparation—that’s probably already done. Simply visit the Opentrons protocol library and download kit protocols from common NGS companies including Illumina, IDT, Qiagen, and more. Choose Opentrons-validated or manufacturer-validated protocols to gain immediate confidence, or choose community-validated protocols to accurately replicate the methods of other scientists.
Protocol library

Flex NGS Workstation

Save time when you choose a Flex NGS workstation—everything you need to automate your workflow so you can conduct science faster. With Flex NGS workstations, you can automate workflows at the scale you need to increase efficiency, reduce errors, and save hands-on time.

Key Considerations for Automated NGS Library Preparation – Live Demonstration

Automated NGS library preparation is an important component of expanded genomics workflows and offers multiple benefits, such as reduced hands-on time, improved sample-to-sample consistency, and reduced errors. Welcome to this webinar where we will outline some important considerations for automated NGS library preparation and then demonstrate the NGS workflow on the Opentrons Flex NGS Workstation, a benchtop liquid handler with 96 sample throughput.
Watch the webinar

Application Notes
Ultra-efficient automated whole-genome sequencing

Library preparation workflow using QIAseq? FX DNA Library Kit and QIAseq? Normalizer Kit on Opentrons Flex

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Technical Bulletin
Opentrons Flex: Flexible automation for NGS

Platform overview and application data for NGS library preparation, nucleic acid extraction, and bead-based protein purification

Download app notes

Flex greatly helps us with library preparation and saves time and effort. More importantly, it changes the way we think about things. How can we systematize this so that it can be done more efficiently overall? Overall I like the culture. It's a more efficient way of doing things.
– Peter McCaffrey, MD, FC AP, assistant professor: UTM B, CTO: PR AGMA bio

Resource

Reagents	application data	protocol	confirm *
Q IA gen Q IA porn FX DNA library kit	automating DNA library prep Q IA pornFX plus normalize Ron open TR ONS flex	Q IAS EQ FX DNA library kit Oh no spray TR ONS flex Q IAS EQ FX DNA library kit with Q IA porn normalizer B oh no squirt TR ONS workflow flex	open TR ONS partner
Q IA gen Q IA porn library normalizer kit	automating DNA library prep Q IA pornFX plus normalize Ron open TR ONS flex	Q IAS EQ library normalizer kit workflow Aoh no spray TR ONS flex	open TR ONS partner
IL lumina DNA prep kit	automated library preparation of small genomes using DNA preparation kit so not-2	IL lumina DNA PR EPON open TR ONS flex (coming soon) IL lumina DNA P repo not-2	open TR ONS
ID TX gen DNA EZ press DMC library prep kits		ID TX gen DNA EZ library prep kit oh no spray TR ONS flex ID TX gen DNA EZ library pre PK ITO not-2 ID TX gen DNA MC library pre PK ITO not-2	open TR ONS
ID TX gen normal A color module		n GS library normalization with ID TX gen normal A color module o not-2	open TR ONS
pornwell express PL ex library prep kit	[poster] automated, multiplexed preparation of up to 384 plasmid libraries supports A 24-hour end-to-end sequencing workflow	Porn well express PL ex library preparation oh no squirt TR ONS flex (2-part protocol)	open TR ONS partner
RO Cheka PA hyper prep kit	automated library preparation of small genomes using DNA preparation kit so not-2	RO Cheka PA hyper prep kit Oh no spray TR ONS flex (coming soon)RO Cheka PA hyper pre PK ITO not-2	open TR ONS
NE B next ultra II DNA library prep kit for IL lumina	automated library preparation of small genomes using DNA preparation kit so not-2	NE B next ultra II DNA library prep kit for ILLUMINAO not-2	open TR ONS partner
NEX TERA XT DNA library preparation kit		IL lumina nexterax to not-2 (4-part protocol)	community

*Verification Category:

Opentrons = Validated by the Opentrons team, verifying that the scientific results are as expected.
Partner = Verified by our reagent partners, verifying that scientific results are as expected.
Community = protocol used by members of the Opentrons community.

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Welcome to make an appointment for an online demonstration to discuss your experimental needs with a team of professional application scientists.

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Automated NGS library preparation

Download from protocol library

Flex NGS Workstation

Key Considerations for Automated NGS Library Preparation – Live Demonstration

Ultra-efficient automated whole-genome sequencing

Opentrons Flex: Flexible automation for NGS

Resource

Learn more