The pipetting workstation is an instrument used in the biological field. It realizes intelligent operation management, modular process design, flexible table layout and graphical operation interface; it is powerful, simple and effective, and the experimental results are reliable. It also frees researchers from complex experimental operations and saves costs. The following introduces the basic knowledge of pipetting workstations:

1. Composition of the pipetting workstation: The pipetting workstation generally consists of five parts: liquid transfer module, monitoring module, workbench, safety door and software. The main function of the liquid transfer module is to complete liquid handling tasks such as sample gradient dilution, liquid transfer and merging. The monitoring module can monitor the status of the liquid transfer module to ensure the accuracy of liquid transfer. Containers, accessories and integrated related instruments required for experiments can be placed on the workbench. The workstation is equipped with a safety door to prevent contaminants or foreign objects from flying in due to liquid splashing during the experiment, thereby ensuring the safety of the operator. The software can control the liquid transfer workstation and its integrated accessories and equipment to realize liquid transfer, reagent addition, dilution ratio or control various integrated accessories and instruments.

2. Three liquid transfer methods in the pipetting workstation: 1. The principle of air displacement liquid transfer is the same as the liquid transfer gun used in the laboratory; the liquid aspiration work achieved through the air displacement principle includes the use of a spring piston or the use of a high-precision syringe pump Aspirate and drain fluids quantitatively. When using a spring piston for liquid transportation, a certain volume V1 needs to be set. The air is expelled by compressing the piston, and the liquid delivery tip is inserted below the solution level, creating a sealed space between the piston and the liquid level. At this point, the piston returns to its original position. Under the action of atmospheric pressure, the solution is sucked into the liquid transfer tip, and the volume of the solution V2 = the volume of the discharged air V1. 2. Liquid line type This instrument has a long pipe, and the suction and discharge of the liquid are controlled by the hydraulic pressure in the pipe. . The advantage of the liquid circuit type is that the suction head can be cleaned by suction and discharge. Tips are reusable, reducing consumable costs, but may lead to contamination between samples. 3. Non-contact can achieve a liquid transfer range of milliliter/picoliter volume. Non-contact liquid delivery methods include valve dispensing technology, inkjet technology, glass capillary dispensers and ultrasonic droplet generation technology. Some of these technologies were developed for the printing industry but are now important components of microarrays and "lab-on-a-chip" systems in the life sciences.

3. Application of pipetting workstation: 1. Drug screening: whether it is primary screening, secondary screening, or intermediate rearrangement tasks. 2. It can conduct high-throughput automatic screening of lead compounds and potential compound libraries, and effectively complete absorption, metabolism and toxicity experiments. 3. In terms of proteome and cytology analysis, cytokine detection kits can quickly and effectively detect multiple different cytokines in the same type of cells. 4. Provides a complete set of DNA/RNA sample purification and preparation solutions to meet subsequent experimental applications, such as sequencing, PCR amplification, transfection and sequencing product purification.