The pipetting module equipped with the OT-2 automated pipetting robot, as one of its core functions, significantly improves its ability to perform high-precision, automated pipetting operations. This module not only has extremely high precision, ensuring the accuracy of the pipetting process, but also shows a high degree of flexibility and can easily adapt to diverse experimental needs. The pipetting module is the key for the OT-2 automated pipetting robot to achieve efficient and precise pipetting operations.

1. Overview of the pipetting module The OT-2 automated pipetting robot is equipped with single-channel and 8-channel pipettes to achieve precise pipetting in the range from 1 μL to 1000 μL. As key components of the pipetting module, these pipettes ensure the accuracy and stability of pipetting through precise mechanical structures and control systems.

2. Features of the pipetting module 1. High precision: (1) When OT-2 absorbs 1 μL of liquid, the accuracy CV value is ±5%. This level of accuracy is comparable to pipetting equipment that is ten times more expensive on the market. (2) The high-precision pipetting ability makes OT-2 perform well in experiments that require micro-liquid handling, such as experiments in the fields of molecular biology, biochemistry and other fields. 2. High flexibility: (1) The pipetting module of OT-2 supports the flexible combination of single-channel and 8-channel pipettes. Users can choose the appropriate pipette for pipetting operations according to experimental needs. (2) At the same time, the modular design of the plate position of OT-2 also allows the pipetting module to be freely combined and adjusted according to experimental needs to meet diverse experimental needs. 3. High compatibility: (1) The pipetting module of OT-2 has strong compatibility and can be adapted to most reagents and consumables on the market, reducing the user's cost of use. (2) OT-2 also provides a rich online protocol library and Python API development environment, allowing users to easily set up and customize pipetting programs to achieve more diverse experimental applications.

3. Installation process of the pipetting module of the OT-2 automatic pipetting robot 1. Preparation (1) Confirm the equipment status: Make sure that the OT-2 automatic pipetting robot is unlocked and in standby mode, and check whether the equipment has any faults or damage. . (2) Prepare the pipette: Choose a suitable single-channel or 8-channel pipette according to the experimental needs, and make sure it is in good condition. (3) Tool preparation: Prepare necessary tools, such as hexagonal screwdrivers, pipette screws, etc., for use during the installation process. 2. Install the pipette (1) Turn on the device: start the OT-2 automated pipetting robot and connect to its control interface through a computer or mobile device. (2) Select the installation location: Select the location where you want to install the pipette on the control interface (OT-2 usually has multiple brackets that can install pipettes). (3) Fix the pipette: Place the pipette on the selected holder, ensuring that it is aligned with the holder. Secure the pipette to the holder using a hex screwdriver and pipette screw. Be careful not to overtighten the screws to avoid damaging the pipette or holder. For multi-channel pipettes, additional attention may be needed to align and secure the channels. 3. Connect the cable (1) Find the cable interface: Find the cable interface connected to the OT-2 on the pipette (usually a white ribbon cable). (2) Insert the cable: Insert the cable into the interface of the pipette and make sure the connection is firm. (3) Test connection: Test the connection of the pipette on the control interface of the computer or mobile device to ensure that it is correctly connected to the OT-2 automated pipetting robot. 4. Software settings (1) Write a pipetting program: Use the online protocol editor or Python API provided by Opentrons to write a pipetting program and set parameters such as pipetting volume, pipetting speed, and pipetting path. (2) Upload program: Upload the written program to the OT-2 automated pipetting robot, and preview and confirm it through the control interface. 5. Debugging and calibration (1) Preliminary debugging: Run the program for preliminary debugging, and observe the operation of the pipette and the pipetting effect. (2) Calibrate the pipette: If necessary, use a calibration aluminum block or other calibration tools to calibrate the pipette to ensure the accuracy and stability of its pipetting accuracy. 6. Complete the installation (1) Clean up the site: Clean up the garbage and tools generated during the installation process, and keep the work area tidy. (2) Record information: Record the model, serial number, installation date and other information of the pipette for subsequent maintenance and management. However, since the specific models and configurations of the Opentrons OT-2 automated pipetting robot may be different, the above installation steps may need to be adjusted according to the actual situation. During the installation process, it is recommended to refer to the device's user manual or contact Opentrons' technical support team for more detailed guidance.

The pipetting module in Opentrons' OT-2 automated pipetting robot, with its high precision, high flexibility and high compatibility, plays an important role in various experiments that require high-precision pipetting. At the same time, users also need to perform regular maintenance and upkeep on the pipetting module to ensure its long-term stable operation and high-precision performance.