In the fast-paced environment of modern biomedical research, efficient and accurate sample purification is one of the key steps to ensure experimental success and data reliability. Opentrons OT-2 automated pipetting platform brings unprecedented automation solutions to laboratories with its excellent flexibility and programmability.

1. Preparation work 1. Equipment inspection: Make sure that the OT-2 automated pipetting platform and its supporting modules (such as magnetic bead purification module, temperature control module, thermal oscillation module, etc.) have been correctly installed and calibrated and are in good working condition. 2. Preparation of consumables: Prepare enough consumables for nucleic acid purification according to experimental needs, such as magnetic beads, buffers, tips, etc., and ensure that these consumables are compatible with OT-2 equipment. 3. Sample preparation: Process the sample to be purified according to experimental requirements, such as extraction, dilution, etc., and place it in the designated location on the OT-2 platform.

2. Programming 1. Create experimental protocol: Use the software provided by Opentrons (such as Opentrons App) to create an automated experimental protocol. This protocol will guide OT-2 on how to automate various steps of sample purification. 2. Parameter setting: Set the parameters of each step in detail in the protocol, including pipetting volume, pipetting speed, temperature, time, etc. These parameters should be adjusted according to the specific requirements of the experiment and the reagents used.

3. Perform automated purification 1. Load samples: Transfer the sample to be purified from the original container to the reaction tube or plate required for purification through the pipetting arm of OT-2. 2. Magnetic bead binding: If the magnetic bead purification method is used, the magnetic bead purification module is used to bind the magnetic beads to the target molecules (such as DNA, RNA) in the sample. This step may require temperature and time control to optimize binding efficiency. 3. Washing: Remove impurities and non-specific binding molecules through multiple washing steps. During the washing process, OT-2 will automatically remove and mix the buffer, and then use the magnetic force of the magnetic bead purification module to separate the magnetic beads from the liquid. 4. Elution: Elute the target molecules from the magnetic beads and collect the purified samples. This step also requires precise control of pipetting volume and time. 5. Other steps: Depending on experimental needs, thermal shaking, centrifugation and other steps may be required to further optimize the purification effect. The OT-2 can perform these steps by connecting the corresponding modules.

4. Real-time monitoring and adjustment 1. Real-time monitoring: During the automated purification process, the equipment operating status and experimental progress can be observed through the real-time monitoring function of Opentrons software. 2. Abnormality handling: If the equipment fails or an abnormality occurs in the experiment (such as inaccurate pipetting volume, incomplete magnetic bead precipitation, etc.), the experiment should be stopped in time and inspection and adjustment should be made.

5. Result collection and analysis 1. Collect purified samples: After the automated purification procedure is completed, collect the purified samples and perform necessary follow-up processing. 2. Quality testing: Conduct quality testing on the purified samples, such as concentration determination, purity analysis, etc., to ensure that the purification effect meets the experimental requirements. 3. Data analysis: Compare and analyze the purification results with experimental expectations to provide reference for subsequent experiments or research.

OT-2 can efficiently and accurately complete the automated purification of samples, improving experimental efficiency and accuracy. At the same time, due to its customizable modular design, OT-2 can also be flexibly configured and optimized according to experimental needs, providing scientific researchers with more convenient and efficient experimental solutions.

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