The fully automatic pipetting workstation brings disruptive innovation to ELISA experiments. This device, which combines intelligence, automation and precision, not only greatly simplifies the complexity of experimental operations, but also shows extraordinary performance in improving experimental efficiency, ensuring data accuracy and reducing human errors. This article aims to provide an in-depth discussion of how fully automated pipetting workstations can give full play to their unique advantages in the ELISA processing process.

Fully automatic pipetting workstation

1. Experimental preparation 1. Preparation of reagents and materials: Prepare the required solutions and ensure that all reagents are of analytical grade. Prepare an ELISA kit, including 96-well plates, standards, enzyme label reagents, antibody solutions, chromogenic reagents, stop solutions, etc. Prepare pipette tips, adapters, trays and other accessories required for a fully automatic pipetting workstation. 2. Instrument and equipment inspection: Ensure that the fully automatic pipetting workstation, microplate reader, centrifuge and other equipment are in good working condition. According to the experimental needs, set the parameters of the fully automatic pipetting workstation, such as pipetting volume, pipetting speed, etc.

2. Sample processing 1. Sample weighing and dilution: According to the experimental requirements, weigh an appropriate amount of sample (for example, solid samples need to be crushed and then sieved, and liquid samples need to be shaken before sampling). Add the sample to the centrifuge tube and add an appropriate amount of extraction solution for dilution. 2. Sample homogenization and centrifugation: Use a oscillator or grinder to homogenize the sample. The homogenized sample was centrifuged to obtain the supernatant.

3. Operation of the fully automatic pipetting workstation 1. Loading of reagents and samples: Place the processed samples, standards, enzyme label reagents, antibody solutions, etc. in the trays and adapters of the fully automatic pipetting workstation. 2. Automatic pipetting and sample addition: The fully automatic pipetting workstation automatically takes the tip according to the preset program, draws the standard or processed sample, and accurately adds it to the designated well position of the 96-well plate. Add enzyme label reagents, antibody solution and other reagents in sequence. 3. Incubation and washing: Place the 96-well plate with reagents in a temperature-controlled incubator and incubate according to the set temperature and time. After the incubation, use a fully automatic pipetting workstation combined with a plate washer to wash the 96-well plate to remove unbound reagents and impurities. 4. Color development and termination: Add chromogenic reagent to the 96-well plate and incubate in the dark at room temperature for a period of time. After the incubation, stop solution was added to terminate the color reaction.

4. Result detection and analysis 1. Absorbance measurement: Use a microplate reader to measure the absorbance value of each well of the 96-well plate at a set wavelength (such as 450nm or 630nm). 2. Data processing: Input the measured absorbance value into the data processing software to draw the standard curve and calculate the sample concentration. Based on the standard curve and sample absorbance value, the concentration of the target molecule in the sample is calculated.

5. End of experiment and cleaning 1. Experiment record: record the experimental results, including standard curve, sample concentration and other information. 2. Equipment cleaning: Use appropriate detergents to clean the fully automatic pipetting workstation and related equipment to ensure the smooth progress of the next experiment.

Performing ELISA processing through a fully automatic pipetting workstation can greatly improve the accuracy and efficiency of experiments and reduce the risk of human error and cross-contamination. At the same time, the fully automatic pipetting workstation also has high-throughput processing capabilities and can complete the detection of a large number of samples in a short time.

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