In the research of molecular biology, extracting, purifying and analyzing DNA are essential basic steps. Gel recovery technology is an important method for isolating and purifying specific DNA fragments. Its efficiency and accuracy directly affect the success of subsequent experiments. As a fully automated pipetting device, the Opentrons fully automatic pipetting workstation can handle gel recycling efficiently and accurately. The following will introduce the entire process of the Opentrons fully automatic pipetting workstation for gel recycling:

Opentrrons fully automatic pipetting workstation

1. Preparation work 1. Equipment preparation: Make sure that the Opentrons pipetting workstation is in good working condition, connect the power supply and turn it on. According to the experimental needs, configure pipette tips, reagent tanks, centrifuge tubes, etc. 2. Reagent preparation: Prepare the Buffer solution used to dissolve the gel. Prepare the purification column, washing Buffer, elution Buffer and other reagents. 3. Sample preparation: Place the gel plate after electrophoresis under UV light, and use a blade to cut out the gel strip containing the target DNA fragment.

2. Gel dissolution and sample loading 1. Gel dissolution: Put the cut gel strip into a 1.5ml centrifuge tube, add an appropriate amount of dissolution Buffer to ensure that the gel is completely submerged. Use a water bath or metal bath to heat the centrifuge tube to an appropriate temperature (such as 55°C) to completely dissolve the gel. 2. Load the sample to the purification column: Transfer the dissolved gel solution to the purification column, taking care to avoid bubbles. Use Opentrons pipetting workstations to precisely control pipetting volumes and ensure consistent loading every time.

3. Washing and centrifugation 1. Washing: Add an appropriate amount of washing Buffer to the purification column to remove impurities and unbound DNA. Use Opentrons pipetting workstations to add and remove washing solutions. 2. Centrifuge: Put the purification column into a centrifuge and centrifuge it at an appropriate speed (such as 12000 rpm) for a certain time (such as 1 minute) to remove the filtrate. Repeat the washing and centrifugation steps until the washing buffer is completely drained.

4. Elution and collection 1. Elution: Add an appropriate amount of elution Buffer to the purification column to elute the target DNA from the purification column. Use Opentrons pipetting workstations to precisely control the amount of eluent added. 2. Collection: Collect the eluted solution into a new 1.5ml centrifuge tube. The eluate can be transferred from the purification column to the collection tube using the Opentrons pipetting station.

5. Concentration determination and storage 1. Concentration determination: Use instruments such as a spectrophotometer or fluorescence quantifier to determine the concentration of recovered DNA. Record the measurement results for use in subsequent experiments. 2. Storage: Store the recovered DNA solution at an appropriate temperature (such as -20°C) for subsequent experimental use.

6. Precautions 1. Operating specifications: Follow the operating instructions of the Opentrons pipetting workstation and gel recovery kit. Take care to avoid cross-contamination and sample mix-ups. 2. Equipment maintenance: Regularly clean and calibrate the Opentrons pipetting workstation to ensure its accuracy and stability. 3. Safe operation: Pay attention to personal protection during operation and avoid contact with harmful substances.

Opentrons fully automatic pipetting workstation integrates advanced robotic technology, open source software and artificial intelligence tools to provide laboratories with efficient, compatible and standardized solutions. During the gel recovery process, this workstation can automatically complete every step from gel cutting, sample processing to DNA elution and collection, greatly improving the efficiency and accuracy of experiments.

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