亚洲国产精品成人综合久久久久久久久精品免费看片国产欧美久久久久久精品一区二区三区_成人精品一区二区91毛片不卡_99久久精品无码一区二区毛片免费_亚洲国产成人精品女人久久久国产美女久久久

下一代實(shí)驗(yàn)室自動(dòng)化就在這里! 了解全新 Opentrons Flex 適合您實(shí)驗(yàn)室的所有方式

Gradient dilution

Many biological or chemical experiments involve substances with a wide concentration range, in which case gradient dilution is a commonly used technique. As with direct dilution, ensuring accurate pipetting volumes is critical. However, it is easy to make mistakes when multiple pipetting steps are involved.

Interested in automated dilution gradients?

No matter your method or throughput, we have the right automation equipment for your laboratory. Watch our How to Choose the Right Automation for Your Lab webinar to learn which platform is best for you.

Click to view

Common reasons why experiments fail

Although in principle, preparing gradient dilutions is relatively simple, many subsequent experiments fail due to errors in three ways:

Inaccurate pipetting——Due to each dilution in gradient dilution being based on the previous dilution, inaccurate pipetting or pipetting effects may be amplified as gradient dilution progresses
wrong hole——Gradient dilution involves multiple pipetting steps, moving in or out of specific wells in a complex pattern, making it more prone to errors than typical experiments
Mix inconsistently——Incomplete mixing at one concentration level can affect all subsequent concentrations, as each concentration is based on the previous one

Save time and avoid mistakes

To avoid the hassle and errors associated with manual pipetting, consider a liquid handling workstation. Protocols can be easily created using the Opentrons Protocol Library - a free collection of protocols that provides downloadable configurations to meet specific volume requirements. Opentrons Flex custom gradient dilution protocols ensure consistent and accurate dilution results, eliminating the repetitive and labor-intensive work of manual pipetting.

View Agreement

Gradient dilution vs. direct dilution

Both gradient dilution and direct dilution produce a range of concentrations. However, their main difference lies in their methods:

Gradient dilution: Each concentration is produced by diluting the previous concentration. This method can achieve precise dilution over a larger concentration range. For example, if the reserve solution in the first column is diluted 11 times (with a coefficient of 3 each time), then the dilution factor in the last column is 3 (11)=177147 times.
direct dilution：All concentrations are extracted in different amounts from a single stock solution. This method is easier to set up and there is no risk of errors affecting all subsequent concentrations.

Agreement Highlights

Opentrons provides open source protocols for OT-2 and Opentrons Flex to help you automate serial dilutions.

customizable serial dilution for OT-2
serial dilution for protein quantification
standard serial dilution

Learn more

Feel free to contact us and our team of professional application scientists will help you confirm whether the automated experimental process is suitable for your needs. If you need to check the operation of the experimental process, you can schedule an online demo with us to discuss your experimental needs in depth with the expert team.

Book a Demo

Contact Us

The experienced service team and strong production support team provide customers with worry-free order services.