Application Advantages

Advantages of using Opentrons Flex? with NucleoMag? Tissue Kit for nucleic acid purification:

1. The method has been verified to ensure the accuracy of the fully automatic extraction and purification process. 2. The yield and purity are reproducible, and genomic DNA can be recovered stably. 3. Strong processing capacity, can process up to 96 samples at the same time. 4. You can visit the Opentrons protocol library or obtain more detailed information through MACHEREY NAGEL's automation technical support [email protected].

Introduction

Extracting genomic DNA is a common experimental process in life sciences and applied testing laboratories, especially in downstream applications such as genotyping testing, sequencing, and clinical research, where stringent requirements for DNA quality and purity are required. MACHEREY NAGEL's NucleoMag? Tissue Kit provides a rapid magnetic bead-based solution for genomic DNA purification. The streamlined design of this kit for use in 96-well plate format provides high-quality, high-purity DNA template for PCR, NGS, or other enzymatic reactions. Combined with the Opentrons Flex? Nucleic Acid Extraction Workstation, HeLa cell genomic DNA can be efficiently purified. Equipped with an Opentrons Flex? ring magnetic stand, a heated shaking module, and 5-1000 μL single- and 8-channel pipettes, with an optional 96-channel pipette, the workstation is suitable for DNA extraction from a variety of sample types, including other eukaryotes cells or tissues. The Opentrons Protocol Library provides protocols for use with the Opentrons Flex? Nucleic Acid Extraction Workstation and the NucleoMag? Tissue Kit, which can be downloaded and used directly.

Application Note NucleoMag? Tissue with Opentrons Flex?

The principle of the NucleoMag? Tissue Kit is based on the reversible adsorption of nucleic acids onto NucleoMag? B-beads under specific binding conditions. When working with tissue samples up to 20 mg or 1×10^6 cells, proteinase K and lysis buffer T1 are added first, followed by the addition of buffer MB2 to promote reversible binding of nucleic acids to magnetic beads. Subsequently, after magnetic bead adsorption, washing was performed with wash buffers MB3 and MB4 to remove salts and other impurities. This is followed by a drying step or MB5 wash, and finally the DNA is eluted using elution buffer MB6. All pipetting and magnetic bead purification steps are performed by Opentrons Flex?.

Application Data

Extraction of DNA from HeLa cells

Using the Opentrons Flex? workstation with the NucleoMag? Tissue kit, high-purity DNA was successfully extracted from 2.4×10^5 HeLa cells. Evaluation of the purified DNA using TapeStation, including assessment of DNA integrity (DIN) and fragment size distribution (virtual gel imaging), showed that the average DIN value of the DNA was 9.0 ± 0.09. This demonstrates that the Opentrons Flex? Workstation combined with the NucleoMag? Tissue Kit can reliably extract high-quality DNA from HeLa cells.

qPCR test results you can trust

qPCR analysis (indicated by blue bars) was performed using PowerTrackTM SYBR GreenMaster Mix as reagent with primers designed by IDT to target a 190 bp region of β-2-microglobulin in the genome. qPCR results showed that amplified fragments were stably detected in all samples tested, demonstrating that this automated nucleic acid purification process is suitable for a variety of common enzyme-based downstream applications. These results demonstrate the reliability of DNA extracted using the Opentrons Flex? Workstation with the NucleoMag? Tissue Kit in qPCR assays.

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