Product Introduction The Opentrons magnetic module is a module that uses magnetic bead separation technology and is used in experiments such as magnetic bead-based nucleic acid extraction. It is perfectly compatible with OT-2. The magnetic module is equipped with two independent adjustable panel supports to support standard and deep well plate laboratory equipment. The magnetic plate on the module supports a variety of different magnetic bead products, and the bracket is compatible with standard or 96-deep well plates, with volumes ranging from 10 μL to 2 mL.

Test Data In order to determine the time required to make the supernatant clear using the Opentrons magnetic module, we conducted rigorous evaluation tests. All tests are based on the default height (18mm) of 8 types of magnetic beads. Each test was performed on NEST 96-well plates. For each bead type, we will measure data in 4 volumes, namely: 20μl, 30μl, 40μl, and 50μl. Within each volume, wells A1, A6, A12, D6, E7, H1, and H12 were filled to determine how granulation changes throughout the plate. Figure 1 shows the beginning of the test (0th minute). We took images of the plate every 1 minute and observed the liquid in the wells until all wells had clear supernatants (shown in Figure 2). The results of extraction of clean beads are shown in Table 1. The results of NGS kit beads are shown in Table 2, and the library preparation results are shown in Table 3.

The deep well plate test experiments used 1ml and 2ml deep well plates respectively. Each test uses the same designated hole locations by default. In these experiments, only Omega-Biotek's mago-bind HDQ beads were used. To prepare these magnetic beads, add 400 μL HDQ Binding Buffer and 20 μL mago-bind?Particles HDQ to each well and pipet repeatedly to mix. As with the previous 96-well plate test process, we started from minute 0 (Figure 3), imaged the plate every 1 minute, and observed the wells until all wells had clear supernatants (Figure 4).

The time required for magnetic bead cleaning and precipitation of magnetic beads produced by different companies at different test volumes:

Precipitation time for library bead preparation at default height:

NGS Kit Magnetic Bead Cleaning Time at Default Height

NGS Kit Magnetic Bead Cleaning Time at Default Height

Note: When using heavier magnetic beads, such as Omega-Biotek's Mag-bound HDQ beads, be aware that these beads are heavier and will settle quickly in the test tube. Microbeads and reagents should be thoroughly mixed before use to prevent premature precipitation of magnetic beads in the supernatant, resulting in inaccurate experimental results.

Product Parameters Module power input: 36V, 2A Power adapter input: 100-240VAC, 50/60Hz The weight of a single magnetic module is 1.5kg. All units in the drawings are mm.

Product maintenance 1. Cleaning and disinfection steps (be careful not to disinfect by spraying) 1) Turn off the module. Unplug all cables. 2) Moisten a paper towel with alcohol or bleach and wipe the module. Note that you need to avoid disconnecting electronic devices and power supplies during the wiping process. 3) Dry with a dry paper towel. Be careful not to clean the power button, power input port, or USB port of the magnetic module. 2. Parts that can be wiped with isopropyl alcohol or ethanol 1) Magnet platform 2) Magnet 3. Parts that can be wiped with isopropyl alcohol, ethanol or bleach 1) Module shell/body 2) Sheet metal side back

Original address:Pipette White Paper: Magnetic Modules