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High-throughput DNA sequencing method

High-throughput DNA sequencing, also known as next-generation sequencing technology, is a method that can process large amounts of DNA samples for sequencing at the same time. This method has higher throughput, faster speed and lower cost than traditional Sanger sequencing. High-throughput sequencing technology can sequence hundreds of thousands to millions of DNA molecules at a time, making it possible to conduct detailed and comprehensive analysis of the transcriptome and genome of a species.

高通量dna測(cè)序法

The development of high-throughput sequencing technology began in 2005, when 454 Life Sciences launched the 454 FLX pyrosequencing platform. Subsequently, Illumina and Roche also launched their own sequencing platforms, which represent representatives of high-throughput sequencing technology. High-throughput sequencing technology is widely used and is a leading technology at the forefront of genetic testing. It will play an important role in tumor prevention, tumor treatment, prenatal genetic disease testing, and newborn genetic testing. It can sequence hundreds of thousands to millions of DNA molecules at a time.

Including but not limited to fields such as basic biological research, medical diagnosis, biotechnology and forensic biology. For example, the determination of the complete sequence map of the human genome and the sequencing of the genomes of numerous animal, plant and microbial species are examples of applications of high-throughput sequencing technology. NGS genetic testing has the advantages of high sensitivity and multi-gene parallel detection, and is widely used. It also has the effect of reducing drug side effects.

Not only that, high-throughput sequencing technology also includes different sequencing methods, such as single-end sequencing and paired-end/mate-paired sequencing. The choice of these methods depends on the specific needs of the research and the sample type. Overall, high-throughput DNA sequencing is a revolutionary technology that has greatly promoted the research and development of biology and medicine, enabling research in life sciences to be conducted at an unprecedented speed and scale.

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