The nucleic acid extraction workstation is an advanced equipment used to extract genomic DNA or RNA from samples. It is widely used in basic medicine, clinical medicine, biology, agriculture, forestry, biomedicine, genetic engineering, genetics, medical testing, and food safety. , environmental testing and other fields. The following is a detailed introduction to the nucleic acid extraction workstation:

1. Definition and function

Through integrated design, the nucleic acid extraction workstation realizes a fully automated process from sample processing to nucleic acid extraction and purification. It can efficiently and accurately complete nucleic acid extraction from a large number of samples in a short time, providing high-quality nucleic acid samples for subsequent genetic analysis, disease diagnosis, drug research and development, etc.

2. Main features

1. High degree of automation: The nucleic acid extraction workstation requires almost no manual intervention during the entire extraction process, reducing human errors and improving the accuracy and repeatability of experiments.

2. Easy operation: The user only needs to put the sample into the equipment, set the parameters, and the equipment can automatically complete the extraction work. The operation is very simple.

3. Fast speed: The workstation can complete nucleic acid extraction from a large number of samples within minutes to hours, greatly improving experimental efficiency.

4. High accuracy: The workstation has a built-in precision control system that can accurately control parameters such as time, temperature, and rotational speed of each step to ensure the accuracy of the extraction results.

5. Good repeatability: The processing process of each sample is consistent, ensuring repeatability and comparability between samples.

3. Working principle

The working principle of the nucleic acid extraction workstation mainly includes steps such as sample processing, cell cracking, nucleic acid purification, washing and recovery. In the sample processing stage, the sample undergoes pretreatment steps such as cutting, dehydration, and centrifugation to obtain a fixed amount of sample solution; in the cell cracking stage, the cell membrane is destroyed and the sample is processed at high speed shaking and high temperature by adding cell cracking solution. Release genomic DNA or RNA; in the nucleic acid purification stage, use adsorbent materials to immobilize nucleic acid molecules and remove impurities; in the washing and recovery stage, high-purity nucleic acid samples are obtained by washing with washing buffer and eluting with elution columns.

4. Precautions for use

1. Operating environment: A clean, wind-free, and vibration-free environment should be selected for operation to avoid interference from external factors on the experimental results.

2. Sample processing: Samples should be processed as soon as possible to avoid nucleic acid degradation; care should be taken to maintain low temperature during processing to reduce nuclease activity.

3. Equipment maintenance: Clean, calibrate and maintain the equipment regularly to ensure its normal operation and the accuracy of experimental results.

4. Safe operation: abide by laboratory safety regulations and avoid splashing water or other liquids into the equipment; pay attention to grounding when connecting the power supply to prevent electric shock accidents.

5. Market product examples

There are currently many brands and models of nucleic acid extraction workstations on the market, such as the Flex series and OT-2 nucleic acid extraction workstation of Hechuang Biotechnology Co., Ltd. These products have their own characteristics, some focus on high-throughput processing capabilities, while others emphasize precise control and ease of operation.

6. Summary

The nucleic acid extraction workstation is undoubtedly one of the core equipment in modern molecular biology laboratories. With its excellent automation performance, intuitive and easy-to-use operation interface, efficient running speed, accurate extraction results and excellent repeatability, it has provided Scientific researchers have built a solid technical backing and guarantee, which has greatly promoted the progress and development of molecular biology research.