亚洲国产精品成人综合久久久久久久久精品免费看片国产欧美久久久久久精品一区二区三区_成人精品一区二区91毛片不卡_99久久精品无码一区二区毛片免费_亚洲国产成人精品女人久久久国产美女久久久

NGS automated library construction process

NGS automated library construction refers to the use of automated technology and equipment to carry out a high-throughput, high-efficiency, and high-precision library construction process for DNA or RNA samples. This process covers many aspects from sample processing, DNA/RNA extraction, library construction to quality control, etc., aiming to reduce manual operations and improve experimental efficiency and accuracy.

NGS自動化建庫

1. Sample preparation and DNA/RNA extraction

1. Sample collection: Collect target samples, such as cells, tissues, blood, etc., to ensure the integrity and representativeness of the samples.

2. Sample processing: Select the appropriate processing method according to the sample type, such as cell lysis, tissue grinding, etc., to release DNA or RNA.

3. DNA/RNA extraction: Use appropriate extraction methods (such as phenol-chloroform method, magnetic bead method, column method, etc.) to purify high-quality DNA or RNA from the sample.

2. Library construction

Library construction is the core step of automated NGS library construction, which usually includes the following sub-steps:

1. DNA/RNA fragmentation:

1>Cut DNA or RNA molecules into smaller fragments for subsequent sequencing reactions. Fragmentation can be achieved by physical methods (such as sonication) or chemical methods.

2>Fragment size is usually between 100-1000 base pairs, depending on the sequencing platform and experimental needs.

2. End repair and A-tail addition:

1>Perform end repair on fragmented DNA or RNA to remove end damage and add phosphate groups and 3’ end A tails. These repair steps facilitate subsequent adapter ligation and library amplification.

3. Connect the adapter:

1>Connect the repaired DNA or RNA fragment to a specific adapter (also called an adapter). Aptamers are DNA or RNA fragments containing specific sequences that are used in subsequent PCR amplification and sequencing reactions.

2>In the process of automated library construction, this step is usually achieved through automated pipetting and ligation reactions.

4. Library amplification:

1>Amplify the DNA or RNA fragments connected to the aptamer through PCR amplification to a sufficient amount to form a library that can be used for sequencing.

2> During the amplification process, primers with adapter sequences are used for specific amplification.

3. Library quality control

Library quality control is an important step to ensure library quality and reliability of sequencing results. Commonly used library quality control methods include:

1. Quantitative PCR: used to detect the concentration and amplification efficiency of the library.

2. Polyacrylamide gel electrophoresis: used to evaluate the size distribution of library fragments.

3. Mass spectrometry analysis: used to further verify the quality and purity of the library.

4. Function:

1. Automated operation: Automate sample processing, library construction and other steps through automated equipment to reduce manual intervention.

2. Improve efficiency: The automated process shortens the experimental cycle and improves experimental efficiency.

3. Reduce errors: Reduce errors caused by human operations and improve the accuracy and stability of experimental results.

Contact Us

The experienced service team and strong production support team provide customers with worry-free order services.

静安区| 黄陵县| 瑞丽市| 翁牛特旗| 格尔木市| 恭城| 偃师市| 湾仔区| 大厂| 西城区| 广德县| 桂林市| 白玉县| 宣恩县| 志丹县| 信阳市| 梅河口市| 二连浩特市| 沧州市| 化州市| 都兰县| 分宜县| 河南省| 浏阳市| 农安县| 盐津县| 盐池县| 无为县| 延吉市| 威宁| 满城县| 永定县| 广饶县| 略阳县| 伊金霍洛旗| 神木县| 中牟县| 中宁县| 建湖县| 鹿泉市| 江达县|