Opentrons Flex magnetic bead protein purification workstation plays an important role in BCA (Bicinchoninic Acid, bicinchoninic acid) and Bradford analysis, providing an efficient, accurate and automated solution for protein quantitative analysis. The following is an introduction to the specific application of this workstation in BCA and Bradford analysis:

Opentrons Flex Magnetic Bead Protein Purification Workstation

1. Overview of the Opentrons Flex Magnetic Bead Protein Purification Workstation The Opentrons Flex Magnetic Bead Protein Purification Workstation is based on the principle of magnetic bead extraction and can automatically complete small-scale protein purification and proteomics sample processing. It supports a variety of workflows, including enzymatic or chemical cleavage experiments, protein labeling experiments, BCA and Bradford detection, mass spectrometry pre-processing, and sample preparation. This workstation features stable output, efficient operation, and flexible compatibility. It can process up to 96 samples at a time, greatly improving experimental efficiency and accuracy.

2. Application of BCA analysis in Opentrons Flex workstation The BCA method is a protein quantitative analysis method based on the maximum light absorption of the purple complex formed by protein and Cu^2+ chelation at a specific wavelength. In the Opentrons Flex workstation, the specific applications of BCA analysis are as follows: 1. Reagent preparation: The workstation will automatically pipette and mix reagents A and B (or reagents A, B and C, depending on the reagents) according to the number of samples to be processed. box), prepare enough working reagents. 2. Sample processing: Transfer the working reagents and test samples to each well of the 96-well transparent flat-bottom plate, and then mix and incubate on the Opentrons thermal shaking module. To ensure signal strength for detection, the BCA method usually requires incubation at 37°C. 3. Result analysis: Use a plate reader compatible with Opentrons Flex (such as BioTek Synergy H1 plate reader) to analyze the result plate, usually reading the absorbance value at a wavelength of 570nm.

3. Application of Bradford analysis in Opentrons Flex workstation The Bradford method is a protein quantitative analysis method based on the color change of Coomassie Brilliant Blue G-250 after binding to protein. In the Opentrons Flex workstation, the specific applications of Bradford analysis are as follows: 1. Reagent preparation: Similar to the BCA method, the workstation will automatically prepare the required reagents. 2. Sample processing: Transfer reagents and samples to a 96-well plate and mix on the Opentrons thermal shaking module. Unlike the BCA assay, Bradford protein assays are usually incubated at room temperature. 3. Result analysis: Use a plate reader to read the absorbance value at a wavelength of 600nm.

4. Advantages of Opentrons Flex workstation 1. Automated operation: Automatic pipetting, mixing, incubation and other steps are realized through programming, which greatly reduces manual operation time and improves experimental efficiency. 2. Standardized process: A standardized operating process has been established to ensure that each experiment can obtain stable and reliable results. 3. High-throughput processing: Up to 96 samples can be processed simultaneously at a time, suitable for large-scale protein quantitative analysis. 4. Strong compatibility: Compatible with a variety of common magnetic bead-based reagents and experimental consumables, making it convenient for users to select and use.

Opentrons Flex magnetic bead protein purification workstation has demonstrated excellent performance in BCA and Bradford analysis, and its application prospects are also very broad. This workstation makes protein quantitative analysis more accurate and efficient through highly automated operating procedures and precise control systems. At the same time, its powerful data analysis functions also significantly reduce human errors in experiments and improve the reliability of results.

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