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Laboratory environment requirements for Opentrons Flex Nucleic Acid Extraction Workstation

In modern biological laboratories, the Opentrons Flex nucleic acid extraction workstation is an efficient automated device, and its requirements for the laboratory environment are particularly important. In order to ensure the accuracy and reliability of the nucleic acid extraction process, the laboratory must have certain environmental conditions.

Opentrons Flex 核酸提取工作站對實驗室環(huán)境的要求

Opentrons Flex Nucleic Acid Extraction Workstation

1. Purification level required for cleanliness: For sensitive experiments such as COVID-19 nucleic acid detection, it is recommended that the laboratory set up 100,000-level purification to prevent cross-contamination and ensure the accuracy of experimental results. Wall and floor: The wall should be smooth, dust-free, corrosion-resistant, easy to clean and disinfect, and seamless; the floor should be anti-static, corrosion-resistant, seamless, and leak-free to maintain the cleanliness of the laboratory. 2. Ventilation and air conditioning system Fresh air air conditioning system: The laboratory should adopt a fresh air air conditioning system to avoid cross-contamination. Exhaust system: The exhaust system should all be purified with high-efficiency filters as required to ensure clean air in the laboratory. Airflow organization: Adopt the airflow organization form of upper and lower rows to maintain air circulation and cleanliness in the laboratory. 3. Temperature and humidity control Temperature control: The temperature in the laboratory should be controlled within an appropriate range to ensure the normal operation of the nucleic acid extraction workstation and the stability of the experimental results. Humidity control: Humidity should also be maintained at an appropriate level to prevent deterioration of experimental samples and damage to instruments. 4. Pressure gradient control micro-pressure differential meter and precision automatic control system: The laboratory should control the pressure gradient through a micro-pressure differential meter and precision automatic control system to prevent the entry of external contaminants. 5. Laboratory facilities and equipment test bench: The lower part of the test bench should be left empty without dead corners to facilitate cleaning and disinfection. The experimental bench should be made of stainless steel 304 to ensure its corrosion resistance and durability. Biological safety cabinet: A biological safety cabinet, such as BSC-II-B2 type, should be installed in the sample preparation area to protect experimental personnel from sample contamination. Eyewash device and emergency sprinkler device: The sample preparation area should be equipped with an eyewash device and, if necessary, an emergency sprinkler device to deal with possible chemical or biological contamination. 6. Safety protection and personal protective equipment: Experimenters should wear personal protective equipment such as ultra-clean workbenches, biological safety cabinets, goggles, protective clothing, medical masks N95, disposable latex gloves, etc. to ensure their own safety. 7. Other requirements Laboratory layout: The laboratory layout should be reasonable to avoid cross-contamination of people and logistics. All indicators and parameters in the room should be displayed at the entrance of the laboratory, and a deviation alarm device should be set up. Laboratory management: The laboratory should establish a sound management system, including experimental operating procedures, safety protection measures, emergency response plans, etc., to ensure the safety and normal operation of the laboratory.

Efficient and accurate operation of the Opentrons Flex Nucleic Acid Extraction Workstation requires a strictly controlled laboratory environment. From the strict requirements of cleanliness, to the reasonable configuration of ventilation and air-conditioning systems, to the precise control of temperature, humidity, and pressure gradients, as well as the perfect layout of laboratory facilities and equipment and strict safety protection measures, every link is crucial. .

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