Opentrons OT-2 PCR workstation has successfully replaced the traditional manual operation method with its automated, efficient and accurate features. The purpose of this article is to provide an in-depth introduction to the configuration recommendations of the Opentrons OT-2 PCR workstation, and provide a comprehensive guide for scientific researchers and clinical laboratory personnel to help them configure this efficient tool more effectively, thereby optimizing the experimental process and improving work efficiency and ensure the reliability and repeatability of experimental results.

Opentrons OT-2 PCR Workstation

1. Recommended core configuration 1. OT-2 automated pipetting platform (1) Features: desktop level, hardware modularity, flexible and customizable, and can realize the automation of many common experiments. (2) Pipetting accuracy: ±5% at 1 μL, meeting the needs of high-precision PCR experiments. (3) Pipetting range: 1~1000μL, covering the pipetting needs of common PCR experiments. 2. High-precision pipette (1) PIPETTE GEN2: A new high-precision pipette dedicated to OT-2. Single-channel and eight-channel pipettes can be installed on the pipetting arm at the same time to improve experimental efficiency. (2) Performance: Able to complete filling of a 96-well plate in 22 seconds, suitable for high-throughput PCR experiments. 3. Function module (1) Temperature control module: used to control the temperature of the PCR reaction to ensure the consistency of experimental conditions. (2) Magnetic bead purification module (optional): used for purification of PCR products to improve the accuracy of experimental results. (3) Thermal cycle module: Provides stable thermal cycle conditions and is a key component of PCR experiments. (4) Other modules: such as HEPA module, thermal oscillator, etc., can be selected and configured according to experimental needs. 4. Experimental equipment and consumables (1) 96-well plate: used for sample placement and pipetting operations in PCR reactions. (2) Tip: Used in conjunction with pipettes to ensure the accuracy and consistency of pipetting. (3) Reagents: Choose verified PCR reagents, such as reagents from brands such as Illumina and Swift/IDT, to ensure the reliability of experimental results.

2. Recommended software configuration 1. Opentrons protocol designer function: Provides a zero-based and easy-to-use protocol design tool. Users can complete the editing and design of experimental steps through graphical instructions. Advantages: No programming foundation is required to generate automated protocols that meet experimental needs. 2. Opentrons Library function: Provides an online free protocol library, and users can download the protocol for use according to their needs. Advantages: It facilitates users to quickly obtain and share experimental protocols and improves experimental efficiency. 3. Python API function: For users with programming capabilities, custom protocols can be written through the Python API. Advantages: Provides higher flexibility and customization to meet complex experimental needs.

3. Other configuration suggestions 1. The laboratory environment ensures that the laboratory has stable power supply and appropriate temperature and humidity conditions to ensure the normal operation of the OT-2 PCR workstation. 2. Training and technical support: Participate in online or on-site training provided by Opentrons and become familiar with the operation and maintenance of the workstation. Use the free online guidance and technical support provided by Opentrons to solve problems encountered during the experiment.

Opentrons OT-2 PCR workstation is an innovative tool in the field of modern biological research and clinical diagnosis. Its automation, efficiency and accuracy have undoubtedly brought unprecedented convenience and efficiency to PCR experiments. Through detailed configuration recommendations, we aim to provide scientific researchers and clinical laboratory personnel with a clear and comprehensive guidance to help them rationally select and configure this efficient tool according to their own needs.

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