OT-2 magnetic bead protein purification workstation, as an outstanding representative of modern biotechnology, integrates magnetic bead separation, automated operation and intelligent control. It can also efficiently and accurately purify recombinant expressed proteins, dealing with natural proteins, membrane proteins, low-abundance proteins, etc. The purification challenges of many complex proteins such as high-density proteins. This article will deeply explore the application of the OT-2 magnetic bead protein purification workstation in different types of protein purification, and reveal how it can help scientific researchers continue to move forward on the road of exploration in life sciences.

OT-2 magnetic bead protein purification workstation

1. Recombinant expressed proteins: Recombinant proteins with affinity tags (such as His tag, GST tag, MBP tag, etc.). These tags can bind to specific ligands on magnetic beads to achieve efficient purification. 2. Natural proteins: By selecting appropriate magnetic beads (such as magnetic beads based on specific binding of antibodies, antigens, receptors, etc.), natural proteins in complex biological samples can be purified. 3. Membrane proteins and intracellular proteins: The OT-2 workstation can effectively process cell lysates and separate and purify membrane proteins and intracellular proteins. These proteins are often difficult to purify due to their hydrophobicity and complexity. 4. Low-abundance proteins: With its efficient separation and enrichment capabilities, the OT-2 workstation can effectively extract low-abundance proteins from complex samples, which is particularly important for studying rare diseases or low-expression biomarkers. 5. Protein complexes: Through a multi-step affinity purification strategy, the OT-2 workstation can purify protein complexes, which is of great significance for studying interactions between proteins. 6. Post-translationally modified proteins: Using specific magnetic beads, such as those targeting phosphorylation, glycosylation and other modifications, proteins that have undergone post-translational modifications can be purified, which is crucial for understanding the protein function regulation mechanism. 7. Organelle proteins: With the help of subcellular organelle separation technology and magnetic bead purification, the OT-2 workstation can purify proteins in specific organelles (such as mitochondria, nucleus, endoplasmic reticulum, etc.).

Although the OT-2 magnetic bead protein purification workstation has wide applicability, in practical applications it is still necessary to select magnetic beads, optimize buffer conditions, and adjust the purification strategy based on the characteristics of the target protein and experimental needs. In addition, strictly following good experimental operation and quality control principles is the key to ensuring purification effect and accuracy of experimental results.

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