亚洲国产精品成人综合久久久久久久久精品免费看片国产欧美久久久久久精品一区二区三区_成人精品一区二区91毛片不卡_99久久精品无码一区二区毛片免费_亚洲国产成人精品女人久久久国产美女久久久

real-time quantitative PCR

Real-time quantitative PCR, the full name of Real-time Quantitative PCR (qPCR for short), is a molecular biology experimental technology that combines PCR amplification technology and fluorescence detection technology, and can perform real-time detection during the PCR amplification process. Monitor and quantify the copy number of specific DNA or RNA sequences.

實(shí)時(shí)定量PCR

1. Advantages and disadvantages of real-time quantitative PCR

advantage:

1. High sensitivity and specificity: qPCR can detect very low concentrations of nucleic acid molecules and accurately identify target sequences. This makes it particularly important in clinical diagnostics, such as detecting viral load.

2. Strong quantitative ability: Through the accumulation of fluorescence signals, the progress of the reaction can be monitored in real time, thereby achieving absolute or relative quantification.

3. Fast and efficient: Compared with traditional PCR, qPCR can complete the detection of a large number of samples in a shorter time, usually within one hour.

4. Intuitive data: Through real-time curve graphs, researchers can intuitively observe the amplification curve, which facilitates analysis and result interpretation.

shortcoming:

1. Higher cost: qPCR instruments and fluorescent dyes are relatively expensive, and the running costs are also high.

2. High technical requirements: operators need to have certain professional knowledge and skills to avoid experimental errors.

3. Susceptible to contamination: Due to its high sensitivity, sample contamination may lead to inaccurate results, so strict experimental condition control is required.

2. Equipment composition of real-time quantitative PCR

The real-time quantitative PCR system mainly consists of the following parts:

1. qPCR instrument: This is the core equipment, equipped with a fluorescence detection system, used to monitor changes in fluorescence signals during the PCR reaction.

2. Thermal cycler: Controls the temperature changes of the reaction system to achieve denaturation, annealing and extension of DNA.

3. Optical detection system: used to detect the fluorescence signal in the reaction tube and convert it into an electrical signal for analysis.

4. Software: used for data collection, processing and analysis, and generating results such as amplification curves and standard curves.

3. Operation method of real-time quantitative PCR

The operation method mainly includes the following steps:

1. Sample preparation: Extract the DNA or RNA in the sample, and convert the RNA into cDNA through reverse transcriptase.

2. Reaction system preparation: including the mixing of primers, probes or dyes, dNTPs, Taq enzymes, buffers and template nucleic acids.

3. Reaction condition setting: Set appropriate PCR reaction procedures according to specific experimental requirements, including pre-denaturation, cycle denaturation, annealing and extension steps.

4. Data collection and analysis: At the end of each cycle, the instrument will automatically record the fluorescence signal, and perform data analysis through the software to calculate the starting nucleic acid amount of the sample.

Real-time quantitative PCR has a wide range of applications in scientific research and clinical diagnosis. Although it has a certain cost and technical threshold, its unparalleled accuracy and efficiency make it an irreplaceable tool. For scientific researchers and medical workers, mastering the operating procedures and equipment usage of this technology is the key to improving experimental efficiency and data accuracy.

Contact Us

The experienced service team and strong production support team provide customers with worry-free order services.

荆门市| 东莞市| 韩城市| 扎鲁特旗| 社会| 娱乐| 昌图县| 巢湖市| 嵊泗县| 栖霞市| 南汇区| 张北县| 临武县| 长宁县| 连山| 丰台区| 晴隆县| 金寨县| 杨浦区| 道真| 双桥区| 绵阳市| 分宜县| 乌拉特前旗| 巴彦县| 昌黎县| 贵定县| 琼结县| 吕梁市| 武夷山市| 南宫市| 福海县| 交城县| 香港| 萨嘎县| 灵台县| 溆浦县| 紫阳县| 崇明县| 富裕县| 五原县|