Application of automated pipetting workstations in the field of drug research and development
Drug R&D quality control is a general term for the behavior of controlling the quality of pharmaceutical products. As special commodities used to treat diseases and save lives, the quality of drugs is directly related to the life safety and health of patients. In the quality control process of drug development, pipetting instruments are indispensable tools. They are widely used in multiple key steps to ensure the accuracy and reliability of experiments. Here are some specific steps required to use pipetting instruments:
1. Which steps in drug R&D quality control require the use of pipetting instruments 1. Sample preparation: (1) In drug R&D, it is often necessary to prepare sample solutions of various concentrations. Pipetting instruments can accurately measure the required volumes of solvents and solutes to prepare sample solutions that meet the requirements. (2) For example, when preparing a solution of an active pharmaceutical ingredient (API), a pipette needs to be used to accurately measure the volumes of API and solvent to ensure that the concentration of the solution is accurate. 2. Solution dilution: (1) During the drug quality control process, it is sometimes necessary to dilute high-concentration solutions to an appropriate concentration range. Pipetting instruments can accurately measure the volume of highly concentrated solutions and diluting solvents, enabling accurate dilution operations. (2) For example, in drug analysis experiments, the drug solution may need to be diluted to a specific concentration range for subsequent measurement and analysis. 3. Reaction system preparation: (1) In drug synthesis or reaction experiments, various reaction systems need to be prepared. Pipetting instruments can accurately measure the volumes of various reactants to ensure that the composition and proportion of the reaction system are accurate. (2) For example, when synthesizing new drug molecules, the molar ratio of reactants needs to be precisely controlled to ensure the smooth progress of the reaction and the purity of the product. 4. Sample removal: (1) In drug analysis or quality testing experiments, it is often necessary to remove a certain amount of sample from the sample container for measurement. Pipetting instruments accurately transfer the required volume of sample, thereby avoiding sample waste and errors. (2) For example, when measuring drug content, a pipette needs to be used to accurately transfer a certain amount of sample solution from the sample bottle to the measuring instrument. 5. Titration experiment: (1) Titration experiment is a commonly used analytical method in drug quality control. During titration, pipetting instruments are required to accurately measure the volumes of titrant and indicator. (2) For example, when performing an acid-base titration experiment, you need to use a pipette to accurately measure the volume of the acid or alkali solution, and observe the color change of the indicator to determine the titration end point. 6. High-throughput screening: (1) In the drug research and development process, high-throughput screening (HTS) is a method to quickly screen a large number of candidate drug molecules. Pipetting instruments play an important role in high-throughput screening experiments, as they can quickly pipette and dispense large amounts of samples and reagents. (2) For example, when using microplates for high-throughput screening, multi-channel pipettes are required to process samples and reagents in multiple sample wells simultaneously.
2. Application of automated pipetting workstations in the field of drug research and development Opentrons pipetting workstations can meet the above requirements and can also automatically record operating parameters. It usually consists of a pipetting platform, pipette pedestal and various automation functions. Its main function is to help experimenters distribute and transfer liquids accurately and efficiently during experiments. It is equipped with an electronic pipette or a multi-channel pipette, which can complete pipetting of samples and reagents through preset programs or manual operations. It is mainly reflected in the following aspects: 1. High precision and accuracy: pipetting The workstation provides high-precision and accurate pipetting capabilities, which is critical for experiments in drug development that require precise sample and reagent ratios. Ensuring the accuracy of pipetting can ensure the reliability of experimental results, thereby improving the success rate of drug development. 2. High reproducibility and sample stability: The pipetting workstation can ensure that the pipetting process of each sample and reagent is consistent, which helps to reduce the variability between batches and improve the comparability of experimental results. and recurrence rate. In the quality control of drug research and development, high reproducibility means the stability and reliability of experimental results, which helps scientific researchers better evaluate and optimize drug performance. 3. Low cross-contamination: Many biological experiments need to avoid contamination between samples due to the sensitivity of reagents. Opentrons pipetting workstations can greatly reduce the risk of cross-contamination and ensure the accuracy of experimental results through precise design and operating procedures. 4. Multi-channel operation capability: Multi-channel operation can save time and reduce experimental errors. In quality control of drug development, multi-channel pipetting workstations can process multiple samples simultaneously, improving experimental efficiency while maintaining high precision and accuracy. 5. Convenient data recording and traceability: The pipetting workstation has a convenient data recording function, which can record the details and parameter settings of each operation step. This provides a complete record of the experimental process for future data analysis and result verification, ensuring data traceability. In quality control of drug development, data traceability is crucial to ensure the reliability and validity of experimental results. 6. Opentrons also provides a powerful open source community and online protocol library, which contains a large number of online experimental application protocol resources. These protocol resources are designed and developed by the Opentrons scientific research team or the customer's R&D team. After rigorous application testing, the scientific nature of the protocol is guaranteed. Researchers can set parameters online and download and use these protocol resources according to their own experimental needs, thereby accelerating the experimental process and reducing research and development costs.
Equipment such as Opentrons' pipetting workstations play an important role in quality control of drug development. Their high precision, high reproducibility, low cross-contamination, multi-channel operation capabilities, and convenient data recording and traceability make scientific research personnel can carry out drug development work more efficiently.
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