The automatic pipetting machine is an efficient laboratory equipment widely used in biomedicine, chemical industry and other fields. It can complete liquid transfer accurately and quickly, improving experimental efficiency. The working principle of the automatic pipetting machine is to control the movement of liquid by sucking and releasing air, and the operation is simple and convenient. During the experiment, just set the volume and target container, and the machine can automatically complete the pipetting work, avoiding human errors. In addition, the automatic pipetting machine also has multiple functions and can operate in different modes according to experimental needs to meet the requirements of various complex experiments.

1. Working Principle of Automatic Pipetting Machine The working principle of automatic pipetting machine is based on the three processes of liquid suction, discharge and mixing. Users set parameters such as volume, speed and depth of pipetting and discharging through LCD display or PLC programming. Then, the pipetting workstation's pipette tip or needle will automatically absorb the set volume of reactants, and accurately distribute the absorbed reaction materials to different reactors through the control of solenoid valves, air pumps and other equipment. Finally, a mixing operation is performed to thoroughly mix the liquid and distribute it into designated reactors.

2. Advantages and disadvantages of automatic pipetting machines compared with manual pipetting machines 1. Advantages of automatic pipetting machines: (1) Efficiency: Automatic pipetting machines can continuously perform a large amount of liquid pipetting operations, greatly improving experimental efficiency. It can automatically complete pipetting tasks according to preset programs without manual intervention, thus saving labor and time costs. (2) High accuracy: Using computer and precision mechanical control, the automatic pipetting machine can maintain high accuracy and low error rate during the pipetting process. This is particularly important in fields that require high-precision experimental results. (3) Saving manpower: Automated operations reduce manual participation and reduce labor costs. At the same time, it also avoids possible interference and errors caused by human factors on experimental results. (4) Avoid errors: Manual operation is easily affected by environmental, emotional and other factors, but automatic pipetting machines can avoid these effects and ensure the accuracy and consistency of experimental results. (5) Intelligent: Modern automatic pipetting machines are more intelligent and can automatically adjust the amount and sequence of liquid pipetting according to experimental needs to achieve intelligent liquid processing. (6) Environmental protection: Some automatic pipetting machines use more environmentally friendly materials and designs, reducing their impact on the environment. 2. Disadvantages of automatic pipetting machines: (1) Higher price: Compared with manual pipetting machines, automatic pipetting machines are usually more expensive and the cost of use is relatively high. This may put some financial pressure on some laboratories with limited budgets. (2) Requires power support: Automatic pipetting machines rely on electricity to operate, making them inconvenient for use in the wild or in environments with unstable power supply. (3) Maintenance costs: Although automation reduces labor costs, the maintenance and upkeep costs of automatic pipetting machines may be higher. Parts need to be inspected and replaced regularly to ensure proper operation and accuracy. (4) Reliance on professional operation: Although the operation is relatively simple, the setup and programming of the automatic pipetting machine may require certain professional knowledge and skills. This may require the laboratory to be staffed with specialized technicians for operation and maintenance.

3. Maintenance and maintenance of automatic pipetting machines In order to ensure the accuracy and reliability of automatic pipetting machines, regular maintenance and upkeep is required. The following are some key daily maintenance considerations: (1) Regular cleaning: The outside of the pipetting workstation should be cleaned after use, including parts such as tip arms and tips. (2) Check the tightness: Regularly check whether the connection between the tip and the pipette is tight to prevent air leakage. (3) Replace consumables: Regularly replace wearing parts such as suction heads to ensure pipetting accuracy. (4) Calibration accuracy: Calibrate the pipette regularly to ensure that its pipetting accuracy meets the requirements.

As an efficient and accurate laboratory equipment, automatic pipetting machines provide powerful help to scientific researchers and production workers. It not only improves work efficiency and reduces operational risks, but also promotes the development and progress of laboratory technology.