{"id":4299,"date":"2024-09-04T16:37:57","date_gmt":"2024-09-04T08:37:57","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2024-09-04T16:38:56","modified_gmt":"2024-09-04T08:38:56","slug":"quanzidongyiyeshebeigoucheng","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/en\/news\/quanzidongyiyeshebeigoucheng\/","title":{"rendered":"Working principle and composition of fully automatic pipetting equipment"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>Fully automatic pipetting equipment, with its excellent liquid handling capabilities and highly intelligent design, is gradually becoming an indispensable assistant for scientific researchers. These precision instruments integrate advanced mechanical, electronic, sensing and software technologies, and can automatically complete a series of complex operations from liquid absorption, transfer, mixing to distribution, etc., greatly improving experimental efficiency, reducing human errors, and providing scientific In-depth exploration of research provides solid technical support.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":4301,\"width\":\"456px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/09\/image-12.png\" alt=\"\u5168\u81ea\u52a8\u79fb\u6db2\u8bbe\u5907\u5de5\u4f5c\u539f\u7406\u53ca\u6784\u6210\" class=\"wp-image-4301\" style=\"width:456px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>1. Working principle of fully automatic pipetting equipment The working principle of fully automatic pipetting equipment is mainly based on the precise control of the mechanical arm and the precise operation of the pipetting head. After the user sets the pipetting parameters through the device's own control system, the robotic arm will drive the pipetting head to move and operate according to the preset program. The pipetting head realizes the suction and release of liquid through the principle of negative pressure or positive pressure, and combines it with precise displacement control to achieve precise control of liquid volume.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>2. Equipment structure and configuration of fully automatic pipetting 1. Robotic arm: one of the core components of fully automatic pipetting equipment, responsible for driving the pipetting head to move and operate. The accuracy and stability of the robotic arm directly affect the pipetting accuracy and stability of the device. 2. Pipetting head: a component used to directly contact liquids and perform suction and release. Depending on experimental needs, pipetting heads can be configured in single-channel or multi-channel configurations. 3. Control system: including software control interface and hardware control circuit. The user sets pipetting parameters and monitors the experimental process through the software control interface; the hardware control circuit is responsible for converting the user's instructions into actual movements of the robotic arm and pipetting head. 4. Safety protection devices: Safety devices such as overload protection and emergency stop buttons are used to ensure operational safety during the experiment.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>3. Advantages and Disadvantages of Fully Automatic Pipetting Equipment Advantages: Improve experimental efficiency and accuracy: Automated operation reduces human errors and operating time. High flexibility: pipetting procedures can be customized according to experimental needs. High safety: Equipped with safety protection devices to ensure experimental safety. Disadvantages: High equipment costs: High-end fully automatic pipetting equipment is expensive. High skill requirements for operators: they need to be proficient in the use and maintenance of equipment.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>4. Examples of brands and models 1. Main advantages of Flex fully automatic pipetting workstation: (1) High flexibility and wide applicability: it can better adapt to experimental processes and can be integrated into most mainstream projects or experiments in the field of life sciences middle. (2) High-throughput: High-throughput brings convenience and expands the scope of experimental projects. (3) Intelligent operation: Equipped with a 7-inch LCD touch screen, which is easy to operate; it has a one-key calibration function, which makes calibration simple and easy; the software system has intelligent graphic prompts, making the operation simple and easy to understand. (4) Board space upgrade: It has 12 main deck spaces and 4 additional board spaces for placing pipette tips, laboratory equipment and functional modules to meet the needs of scientific researchers. Technical features: (1) Fully automatic rotating plate gripper: installed on the Z-axis gantry, it can grip laboratory equipment and move the rotating plate on modules such as magnetic bead separation plates or thermal cyclers. (2) 96-channel high-precision pipetting: Using air displacement technology, it can carry out high-precision pipetting of 1-1000 \u03bcL; the pipette is equipped with intelligent sensors with automatic calibration, real-time positioning and error detection functions. (3) Flexible pipetting configuration: The Z-axis gantry can be installed with any two single-channel or 8-channel pipettes, or a Flex 96-channel pipette can be configured separately.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>2. Product features of the automated pipetting robot OT-2: (1) Hardware modularity: flexible and customizable, and the plate positions can be freely combined according to needs. (2) High-precision pipetting: It can be equipped with both single-channel and 8-channel pipettes to meet the precise pipetting of 1-1000 \u03bcL; when absorbing 1 \u03bcL of liquid, the accuracy CV value is \u00b15%, and the pipetting accuracy is comparable to expensive pipettes on the market. 10x pipetting equipment. (3) Consumables have strong compatibility: compatible with any laboratory equipment that meets ANSI\/SLAS standards. (4) Flexible program: Provides an online protocol editor that can be used from scratch, with a simple and easy-to-understand visual interface; provides a large number of popular online application resources, which can be easily set up in the Opentrons online protocol library and can be downloaded and used. (5) Program open source: Python API can be used to develop more application processes and provide customized development value-added services. Functional modules: including temperature control module, magnetic bead purification module, thermal cycle module, HEPA module and thermal oscillator, etc. By adding different functional modules, you can enjoy more convenient and powerful automation functions.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>Fully automatic pipetting equipment is one of the important tools for modern laboratory automation. It has significant advantages in improving experimental efficiency, reducing human errors, and ensuring the accuracy of experimental results. With the continuous advancement of technology and the continuous expansion of applications, fully automatic pipetting equipment will play an important role in more fields.<\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>Fully automatic pipetting equipment, with its excellent [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":4301,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-4299","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u5168\u81ea\u52a8\u79fb\u6db2\u8bbe\u5907\u5de5\u4f5c\u539f\u7406\u53ca\u6784\u6210 - 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