{"id":4321,"date":"2024-09-06T18:18:05","date_gmt":"2024-09-06T10:18:05","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2024-09-06T18:20:19","modified_gmt":"2024-09-06T10:20:19","slug":"weikongbanhengwenzhendangqi","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/en\/news\/weikongbanhengwenzhendangqi\/","title":{"rendered":"Microplate constant temperature oscillator"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>A microplate thermostatic shaker is a device used to mix solutions or culture and incubate cells at an appropriate temperature. It combines microprocessing technology and PID control, which can quickly maintain stability after reaching the target temperature, saving waiting time.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":4323,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/09\/image-17.png\" alt=\"\u5fae\u5b54\u677f\u6052\u6e29\u632f\u8361\u5668\" class=\"wp-image-4323\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>1. Working principle of microplate constant temperature oscillator 1. Temperature control system (1) Constant temperature control principle: Heating control: The heating control system of the constant temperature oscillator uses a PID (proportional-integral-derivative) feedback controller to control the reaction system. temperature. Before the experiment, the user will set the required reaction temperature, and the computer and program will control the input of heating power based on this set value. During the heating process, the reaction system will gradually heat up until it reaches and stabilizes at the set temperature. Ventilation and refrigeration control: When the temperature of the reaction system is higher than the set value, the refrigeration system will start. Refrigeration systems usually consist of components such as compressors, condensers, expansion valves, and evaporators. When the refrigerant evaporates in the evaporator, it absorbs the heat in the reaction system, and then removes the cold energy through the action of the compressor and dissipates heat through the condenser. This process is carried out cyclically to keep the temperature of the reaction system constant. (2) Temperature sensor: In order to accurately control the temperature, the constant temperature oscillator has a built-in high-precision temperature sensor. These sensors can monitor the temperature of the reaction system in real time and feed the data back to the control system for timely adjustments. 2. Oscillation system (1) Mechanical structure: The oscillation system is composed of motors, reducers, pendulums, axles and other components. The motor provides power and drives the swing rod to rotate at a constant speed through the reducer. The motion of the pendulum is converted into a back-and-forth swinging motion, creating an oscillating effect. (2) Amplitude and frequency control: By adjusting the length of the pendulum and the mass of the weight, the amplitude and frequency of oscillation can be accurately controlled. This adjustment mechanism allows the constant-temperature oscillator to meet the needs of different experiments for oscillation conditions. 3. Display and operating system (1) LCD display: Modern constant temperature oscillators are usually equipped with an LCD display to display parameters such as current temperature, oscillation speed, and remaining time. This allows users to intuitively understand the operating status of the equipment and make adjustments accordingly. (2) Operation interface: The operation interface is simple and easy to understand. Users can set parameters such as temperature, oscillation speed, and timing through buttons or touch screen. In addition, some advanced models also have an automatic memory function that can save the user's commonly used setting parameters.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>2. Product features of microplate constant temperature oscillator 1. Accurate temperature control: the temperature control accuracy can reach \u00b10.3\u00b0C to \u00b10.5\u00b0C, and the temperature display accuracy is 0.1\u00b0C. 2. Stable oscillation function: The oscillation speed range is wide, such as 100-1200rpm, and the oscillation amplitude is 2mm (horizontal rotation) to ensure uniform mixing of the solution. 3. Easy operation: Temperature, oscillation, time and other parameters can be set and displayed through the LCD display, which is beautiful and easy to operate. 4. Energy saving and environmental protection: It is driven by a DC brushless motor, which has low noise and no pollution. The motor has a long life and is maintenance-free. 5. Safe and reliable: It has multiple safety protection functions, such as overload protection, overheating protection, etc., and complies with CE safety standards.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>3. Specific applications of microplate thermostatic oscillators 1. Mixing of solutions (1) Biochemical reagents: In biochemical experiments, various biochemical reagents often need to be mixed to trigger specific chemical reactions. The microplate constant-temperature oscillator can provide uniform shaking to ensure that reagents are fully mixed and improve the accuracy of experimental results. (2) Medium preparation: In the fields of microbiology, cell biology and other fields, the preparation of culture medium is one of the key steps in experiments. Through the microplate constant temperature oscillator, various components in the culture medium (such as nutrient salts, growth factors, etc.) can be evenly mixed to provide a suitable environment for the growth of microorganisms or cells. (3) Sample processing: Before sample analysis, it is often necessary to pre-process the sample, such as dilution, dissolution, etc. Microplate thermostatic shakers can help process these samples quickly and evenly, providing accurate samples for subsequent experiments. 2. Cell culture and incubation (1) Cell culture plate: Microplate constant temperature oscillator is particularly suitable for oscillation culture of cell culture plates such as 24-well plates, 48-well plates, and 96-well plates. Through appropriate temperature and shaking conditions, the adhesion, growth and proliferation of cells can be promoted and the success rate of cell culture can be improved. (2) Suspension cell culture: For the culture of suspension cells, a microplate thermostatic oscillator is also suitable. Vibration can prevent cells from aggregating and maintain uniform distribution of cells in the culture medium, which is beneficial to cell growth and metabolism. (3) Drug screening and cytotoxicity testing: In experiments such as drug development and cytotoxicity testing, the impact of drugs on cells needs to be evaluated. The microplate thermostatic oscillator can provide constant temperature and oscillation conditions to ensure full contact between the drug and the cells, thereby accurately assessing the effect and toxicity of the drug.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>Microplate constant temperature oscillator, as a powerful assistant in modern scientific research laboratories, with its excellent performance, simple operation process and high safety, continues to help scientific researchers overcome difficulties and promote the in-depth development of scientific research. Whether in molecular biology, cell culture, drug screening, environmental monitoring and other broad fields, it has demonstrated extraordinary practical value.<\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>A microplate thermostatic shaker is a device used to mi [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":4323,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-4321","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u5fae\u5b54\u677f\u6052\u6e29\u632f\u8361\u5668 - 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