{"id":4562,"date":"2024-10-07T18:09:06","date_gmt":"2024-10-07T10:09:06","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2024-10-07T18:10:07","modified_gmt":"2024-10-07T10:10:07","slug":"yiyeqiangyidangerdangqubie","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/en\/news\/yiyeqiangyidangerdangqubie\/","title":{"rendered":"The difference and function of the first and second gears of the pipette gun"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>In the fields of scientific research experiments and biomedicine, pipettes, as an indispensable tool, play a vital role. With its precise operation and efficient performance, it meets the various needs of the laboratory for liquid transfer. However, for pipette users, understanding and mastering the functions and features of its different gears is the key to ensuring experimental accuracy and efficiency. In particular, the first and second gears of the pipette gun each have unique functions and applicable scenarios.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":4564,\"width\":\"558px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/10\/image-4.png\" alt=\"\u79fb\u6db2\u67aa\u4e00\u6863\u548c\u4e8c\u6863\u7684\u533a\u522b\u53ca\u4f5c\u7528\" class=\"wp-image-4564\" style=\"width:558px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>1. The difference between the first speed and the second speed of the pipette gun. First speed 1. Functional features: The first speed is one of the most commonly used speed settings of the pipette gun. It is mainly used to accurately control the movement of liquids. When the button is pressed to the first position, the pipette delivers droplets at a slower rate, allowing for more delicate operations. 2. Application scenarios: Level 1 is suitable for experiments that require high-precision operations, such as molecular biology, cell culture, etc. Because the first speed is slower, it can also reduce the generation of bubbles during liquid movement and ensure the accuracy of experimental results. For operation with viscous liquids, first gear is also more suitable as it allows for better manipulation of such liquids. Second gear 1. Functional features: The second gear is faster than the first gear and is mainly used to improve operating efficiency. When the button is pressed to the second position, the pipette will send out liquid at a faster speed, thus shortening the experiment time. 2. Application scenarios: The second level is suitable for large-volume pipetting or experiments that require rapid liquid movement. In the case of repeated sampling from the same container, using second gear can complete the operation more quickly. It should be noted that when using the second gear, due to the faster speed, the risk of liquid splashing or bubble generation may increase, so careful selection is required based on experimental needs.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>2. The functions of the first and second gears of the pipette gun. Detailed explanation of the functions of the first gear. 1. High-precision control: The first gear speed is slower, and the pipette sends out droplets more slowly, which can control the operation of the pipette more accurately. In situations where very precise operations are required, such as in experimental fields such as molecular biology and cell culture, using the first gear can better control the movement of liquid and ensure the accuracy of the pipetting volume. 2. Reduce the generation of bubbles: During the movement of liquid, especially when the liquid contains surfactants or other ingredients that are prone to bubbles, bubbles are likely to be generated. Using the first speed can avoid the generation of bubbles, because the slower speed reduces the impact force of the liquid, thereby ensuring the accuracy of the experimental results. 3. Suitable for viscous liquids: For the operation of viscous liquids, the first speed is slower and can better control such liquids. Viscous liquids tend to create resistance inside the pipette when moving quickly, resulting in inaccurate pipetting. Using first gear can prevent this from happening. Detailed explanation of the function of the second gear 1. Improve operating efficiency: The second gear is faster, which can significantly improve the operating efficiency and shorten the experimental time. In experiments that require a large amount of pipetting or liquid movement that needs to be completed quickly, such as enzyme-linked immunosorbent assay (ELISA), high-throughput sequencing, etc., using the second gear can complete the task faster. 2. Large-capacity pipetting: The second speed is suitable for the operation of large-capacity pipettes. When a large amount of liquid needs to be transferred, using the second gear can complete the liquid movement faster and improve work efficiency. 3. Convenient sampling: Use the second speed to sample quickly and conveniently, especially when sampling repeatedly from the same container. The fast sampling speed can reduce the operating time of the experimenter and improve the overall efficiency of the experiment.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>The first and second gears of the pipette gun have different emphasis on functions and uses. Which gear to choose depends on the specific needs of the experiment and the characteristics of the liquid. The first gear is suitable for experiments that require high-precision control and reduced foam generation, while the second gear is suitable for scenarios that improve operating efficiency and large-volume pipetting.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>Related reading recommendations<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/5-reasons-to-automate\/\">5 reasons to automate your NGS workflow<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/wsmwmzzlsjszshdqzdrxhy\/\">Why we make the world\u2019s most affordable fully automated thermal cycler<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/ot-2ytcljqraqhfgxx\/\">OT-2 liquid handling robot safety and regulatory compliance information<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/weikongbanzhendangqi\/\">Main components of microplate shaker<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/yiyejixiebijiegou\/\">Structure of the pipetting robot arm<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/yangpinqianchuliyitiji\/\">Sample pre-processing integrated machine<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>In the fields of scientific research experiments and bi [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":4564,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-4562","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - 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