{"id":4838,"date":"2024-11-06T17:53:42","date_gmt":"2024-11-06T09:53:42","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2024-11-06T17:54:11","modified_gmt":"2024-11-06T09:54:11","slug":"jiankugongzuozhanyuanli","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/en\/news\/jiankugongzuozhanyuanli\/","title":{"rendered":"Library construction principle of fully automatic pipetting library construction workstation"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>The library construction principle of the fully automatic pipetting library construction workstation is mainly based on precision liquid control technology and industrial automation control technology. Through automated and intelligent methods, it can achieve efficient and accurate processing of liquid samples, thereby building a high-quality sample library. The following is a detailed explanation of the library construction principle of the fully automatic pipetting library construction workstation:<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":4840,\"width\":\"591px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/11\/image-14.png\" alt=\"\u5168\u81ea\u52a8\u79fb\u6db2\u5efa\u5e93\u5de5\u4f5c\u7ad9\u7684\u5efa\u5e93\u539f\u7406\" class=\"wp-image-4840\" style=\"width:591px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph {\"align\":\"center\"} -->\n<p class=\"has-text-align-center\"><a href=\"https:\/\/opentrons.com.cn\/\">Fully automatic pipetting and library construction workstation<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>1. Liquid control system The core of the fully automatic pipetting and library building workstation is the liquid control system, which is mainly composed of pipetting heads, pump heads, solenoid valves, air pumps and other components. These components achieve precise control of liquid samples through sophisticated mechanical design and highly sensitive sensors. 1. Pipetting head: The pipetting head is a part of the workstation that is in direct contact with the liquid sample. It automatically absorbs and distributes the specified volume of liquid sample according to the preset program. Pipetting heads are usually designed with sample diversity in mind and can handle liquids of different viscosities and volumes. 2. Pump head: The pump head is responsible for providing power to the pipetting head. By accurately controlling the movement of the pump head, precise absorption and distribution of liquid samples can be achieved. The performance of the pump head directly affects the accuracy and stability of pipetting. 3. Solenoid valves and air pumps: Solenoid valves and air pumps are used to control the flow path and speed of liquids to ensure that the liquid can follow preset procedures during the suction, distribution and mixing processes.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>2. Automation control technology The fully automatic pipetting and library building workstation also integrates advanced automation control technology, including PLC programming, sensor technology, machine vision, etc. These technologies together achieve precise control of the liquid handling process. 1. PLC programming: PLC (programmable logic controller) is the brain of the workstation. It controls the coordinated work of each component according to the program set by the user. PLC programming is highly flexible and scalable and can be customized and modified according to experimental needs. 2. Sensor technology: Sensors are used to monitor various parameters in the liquid treatment process in real time, such as liquid level, temperature, pressure, etc. Through the information fed back by the sensor, the workstation can adjust operating parameters in time to ensure the accuracy and stability of the experiment. 3. Machine vision: Machine vision technology is used to identify the position and status of the sample to ensure that the pipetting head can accurately align the sample and operate it. Machine vision technology can also be used to monitor experimental processes in real time to detect and correct potential errors in a timely manner.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>3. Library construction process The library construction process of the fully automatic pipetting library construction workstation usually includes the following steps: 1. Sample preparation: Place the sample to be processed in a designated location and perform preprocessing, such as dilution, mixing, etc. 2. Program setting: Set experimental parameters and steps through the control software of the workstation, including pipetting volume, pipetting times, reaction time, etc. The settings of these parameters need to be customized based on experimental needs and sample characteristics. 3. Automatic operation: Start the workstation, and the control system will automatically complete the steps of sample absorption, distribution, mixing and reaction according to the preset program. During this process, the workstation will monitor changes in various parameters in real time and make adjustments as needed. 4. Data storage and analysis: After the experiment is completed, the workstation will store the experimental results and data in the data storage and analysis system. Users can query, export and analyze data through the software to obtain valuable experimental results and conclusions.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>4. Advantages and Features The fully automatic pipetting and library construction workstation has the following advantages and features: 1. Efficiency: It can automatically complete multiple steps of experimental operations, greatly improving experimental efficiency. 2. Accuracy: By precisely controlling the movement and distribution of liquid, the accuracy of experimental results is ensured. 3. Flexibility: It can be flexibly configured and customized according to experimental needs to meet research needs in different fields. 4. Intelligent: It integrates advanced control systems and data storage and analysis systems to realize intelligent management of experiments.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>The library construction principle of the fully automatic pipetting library construction workstation is mainly based on precision liquid control technology and industrial automation control technology. Through automation and intelligence, the workstation can process liquid samples efficiently and accurately, thereby building a high-quality sample library.<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>Related reading recommendations<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/bpsopentronsgjdyyq\/\">White Paper: Opentrons High-Precision Pipettes<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/opentrons-wkmkjs\/\">Introduction to Opentrons pipette temperature control module<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/zdhpcrdzs\/\">Automated PCR e-book<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/yiyegongzuozhanshuju\/\">What factors will affect the data of the fully automatic pipetting workstation<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/opentrons-flex-shijifenzhuang\/\">Opentrons Flex reagent dispensing workstation workflow<\/a><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/opentrons-flex-hesuantiqu\/\">How to calibrate the Opentrons Flex nucleic acid extraction workstation<\/a><\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>The library construction principle of the fully automat [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":4840,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-4838","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - 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