{"id":516,"date":"2023-02-22T13:00:00","date_gmt":"2023-02-22T05:00:00","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=case&#038;p=516"},"modified":"2023-12-10T23:29:35","modified_gmt":"2023-12-10T15:29:35","slug":"retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2","status":"publish","type":"case","link":"https:\/\/opentrons.com.cn\/resources\/customer-stories\/retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2\/","title":{"rendered":"Retro Biosciences\u4f7f\u7528OT-2\u81ea\u52a8\u5316\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6587\u5e93\u5236\u5907"},"content":{"rendered":"\n<p>\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u80fd\u591f\u4ee5\u5355\u7ec6\u80de\u4e3a\u5355\u4f4d\u8fdb\u884c\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\uff0c\u8fd9\u9879\u7814\u7a76\u53ef\u4ee5\u8ba9\u6211\u4eec\u8fdb\u4e00\u6b65\u4e86\u89e3\u4e00\u4e9b\u7a00\u6709\u4e14\u8d35\u91cd\u7684\u7ec6\u80de\u7fa4\u3002\u5c3d\u7ba1\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f\u7684\u4f7f\u7528\u8d8a\u6765\u8d8a\u5e7f\u6cdb\uff0c\u4f46\u8fd9\u9879\u6280\u672f\u7684\u6210\u672c\u4ecd\u7136\u76f8\u5f53\u6602\u8d35\u3002\u9664\u4e86\u6d4b\u5e8f\u5e73\u53f0\u548c\u6bcf\u6b21\u5355\u72ec\u8fd0\u884c\u7684\u6210\u672c\u5916\uff0c\u6587\u5e93\u5236\u5907\u6b65\u9aa4\u65e2\u6602\u8d35\u53c8\u8d39\u529b\u3002\u7136\u800c\uff0c\u628a\u6587\u5e93\u5236\u5907\u6b65\u9aa4\u8fdb\u884c\u81ea\u52a8\u5316\u53ef\u4ee5\u6709\u6548\u51cf\u5c11\u5b9e\u9a8c\u7ed3\u679c\u7684\u53d8\u5f02\u6027\uff0c\u540c\u65f6\u8282\u7ea6\u7814\u7a76\u4eba\u5458\u7684\u5b9d\u8d35\u65f6\u95f4\uff0c\u89e3\u653e\u4ed6\u4eec\u7684\u53cc\u624b\uff0c\u8ba9\u79d1\u7814\u4eba\u5458\u80fd\u6709\u66f4\u591a\u65f6\u95f4\u7528\u6765\u6765\u8bbe\u8ba1\u5b9e\u9a8c\u6216\u5206\u6790\u6570\u636e\u3002<\/p>\n\n\n\n<p>\u76ee\u524d\uff0c\u5e02\u9762\u4e0a\u80fd\u5b9e\u73b0\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u7684\u81ea\u52a8\u5316\u6587\u5e93\u5236\u5907\u6210\u672c\u9ad8\u8fbe 26\u4e07\u7f8e\u5143\uff0c\u6602\u8d35\u7684\u6210\u672c\u5df2\u7ecf\u8d85\u51fa\u4e86\u5e02\u9762\u4e0a\u5927\u90e8\u5206\u5c0f\u578b\u7814\u7a76\u5b9e\u9a8c\u5ba4\u7684\u9884\u7b97\u3002\u4e3a\u4e86\u5411\u66f4\u591a\u5b9e\u9a8c\u5ba4\u63d0\u4f9b\u8fd9\u9879\u81ea\u52a8\u5316\u6280\u672f\uff0cRetro Biosciences \u6210\u529f\u201c\u7834\u89e3\u201d\u4e86 Opentrons \u7684 OT-2 \u81ea\u52a8\u5316\u79fb\u6db2\u5e73\u53f0\uff0c\u4f7f\u5176\u80fd\u591f\u5b9e\u73b0\u81ea\u52a8\u5316\u6587\u5e93\u5236\u5907\u529f\u80fd\uff0c\u540c\u65f6\u6210\u672c\u4ec5\u4e3a 2.6\u4e07\u7f8e\u5143\uff01\u8282\u7ea6\u4e8610\u500d\u7684\u542f\u52a8\u6210\u672c\u3002<\/p>\n\n\n\n<p><strong>Retro Biosciences<\/strong>\u00a0\u662f\u4e00\u5bb6\u4f4d\u4e8e\u52a0\u5dde\u7684\u751f\u7269\u533b\u836f\u516c\u53f8\uff0c\u4e13\u6ce8\u4e8e\u8840\u6d46\u7597\u6cd5\u3001\u7ec6\u80de\u91cd\u7f16\u7a0b\u548c\u7ec6\u80de\u81ea\u566c\u7814\u7a76\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img decoding=\"async\" src=\"https:\/\/opentrons.com\/wp-content\/uploads\/2023\/02\/retro-bio-OT-2-768x1024.jpeg\" alt=\"Retro Bio's OT-2, hacked to perform scRNAseq at a fraction of the cost\" class=\"wp-image-2381\" style=\"width:490px\" title=\"Retro Bio's OT-2, hacked to perform scRNAseq at a fraction of the cost\"\/><figcaption class=\"wp-element-caption\">Retro Bio \u4f7f\u7528OT-2 \u8fdb\u884c\u5355\u7ec6\u80de\u6d4b\u5e8f\u6587\u5e93\u5236\u5907\uff08\u56fe\u7247\u7531 Retro Biosciences \u7684 Ben \u63d0\u4f9b\uff09<\/figcaption><\/figure>\n\n\n\n<p><strong>Q: \u8bf7\u7b80\u5355\u4ecb\u7ecd\u4e00\u4e0bRetro Biosciences \u516c\u53f8\uff1f<\/strong><br>A: Retro Biosciences \u7684\u6210\u7acb\u6700\u521d\u662f\u4e00\u5bb6\u4e34\u5e8a\u6cbb\u7597\u836f\u7269\u7814\u53d1\u516c\u53f8\uff0c\u540e\u6765\u4e3b\u8981\u4e1a\u52a1\u662f\u5f00\u53d1\u9884\u9632\u6027\u836f\u7269\u6765\u89e3\u51b3\u7ec6\u80de\u8870\u8001\u95ee\u9898\u3002\u5927\u591a\u6570\u7814\u7a76\u8870\u8001\u7684\u751f\u7269\u5b66\u516c\u53f8\u90fd\u4e13\u6ce8\u4e8e\u5355\u4e00\u7684\u8870\u8001\u673a\u5236\uff0c\u800c Retro Biosciences \u662f\u4e00\u5bb6\u591a\u9879\u76ee\u516c\u53f8\uff0c\u6211\u4eec\u52aa\u529b\u63a2\u7d22\u7684\u4e09\u79cd\u7597\u6cd5\u6709\uff1a\u7ec6\u80de\u91cd\u7f16\u7a0b\u3001\u81ea\u566c\u548c\u8840\u6d46\u542f\u53d1\u7597\u6cd5\u3002\u6211\u4eec\u4e86\u89e3\u5230\u533b\u9662\u63a5\u5f85\u7684\u75c5\u4eba\u4e2d\u6709 80% \u4ee5\u4e0a\u7684\u75be\u75c5\u8d1f\u62c5\u6e90\u81ea\u4e8e\u8870\u8001\u673a\u5236\uff0c\u56e0\u6b64\u6211\u4eec\u5e0c\u671b\u627e\u5230\u80fd\u76f4\u63a5\u89e3\u51b3\u8fd9\u4e9b\u95ee\u9898\u7684\u7597\u6cd5\uff0c\u5e2e\u52a9\u66f4\u591a\u75c5\u4eba\u3002<\/p>\n\n\n\n<p><strong>Q:<\/strong>\u00a0<strong>\u81ea\u52a8\u5316\u5982\u4f55\u5e2e\u52a9\u60a8\u5b9e\u73b0\u76ee\u6807\uff1f<\/strong><br>A: \u673a\u5668\u81ea\u52a8\u5316\u662f\u4e0d\u77e5\u75b2\u5026\u7684\uff0c\u53ef\u4ee5\u6301\u7eed\u6027\u5de5\u4f5c\uff0c\u540c\u65f6\u81ea\u52a8\u5316\u7684\u6548\u7387\u663e\u8457\u8d85\u8d8a\u4eba\u5de5\u3002\u6211\u4eec\u6b63\u5728\u63a2\u7d22\u672a\u77e5\u7684\u751f\u7269\u5b66\uff0c\u5176\u4e2d\u4e00\u4e9b\u63a2\u7d22\u6d89\u53ca\u5230\u7684\u53c2\u6570\u4ee5\u53ca\u53c2\u6570\u7ec4\u5408\u975e\u5e38\u5e7f\u6cdb\uff0c\u5982\u679c\u7ee7\u7eed\u8ba9\u79d1\u7814\u4eba\u5458\u4f7f\u7528\u624b\u52a8\u7684\u65b9\u5f0f\u8fdb\u884c\u5b9e\u9a8c\uff0c\u6211\u8ba4\u4e3a\u8fd9\u662f\u5bf9\u7814\u7a76\u8005\u79d1\u5b66\u5934\u8111\u7684\u4e00\u79cd\u6d6a\u8d39\u3002<\/p>\n\n\n\n<p><strong>Q: \u60a8\u4e3a\u81ea\u52a8\u5316\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u7684\u6587\u5e93\u5236\u5907\u521b\u5efa\u4e86\u4e00\u4e2a\u975e\u5e38\u9177\u4e14\u975e\u5e38\u5b9e\u60e0\u7684\u89e3\u51b3\u65b9\u6848\u3002\u8bf7\u544a\u8bc9\u6211\u4eec\u60a8\u521b\u9020\u8fd9\u4e2a\u65b9\u6848\u7684\u521d\u8877\uff0c\u4ee5\u53ca\u60a8\u662f\u5982\u4f55\u505a\u5230\u7684\uff1f<\/strong><br>A: \u6587\u5e93\u5236\u5907\u5bf9\u6211\u4eec\u6765\u8bf4\u662f\u4e00\u4e2a\u5165\u95e8\u9879\u76ee\u3002\u81ea\u52a8\u5316\u7684\u6700\u5927\u4f18\u52bf\u662f\u4f60\u53ef\u4ee5\u5c06\u90a3\u4e9b\u56fa\u6709\u7684\u3001\u5927\u91cf\u91cd\u590d\u7684\u3001\u6700\u4e0d\u5e0c\u671b\u51fa\u9519\u7684\u73af\u8282\u81ea\u52a8\u8fd0\u4f5c\u8d77\u6765\uff0c\u8282\u7ea6\u65f6\u95f4\uff0c\u51cf\u5c11\u4eba\u5de5\u5931\u8bef\u3002\u6211\u4eec\u5728\u8fd9\u91cc\u505a\u4e86\u5f88\u591a\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u3002\u6bcf\u4e00\u6b21\u6d4b\u5e8f\u6211\u4eec\u9700\u8981\u9762\u4e34\u7684\u5206\u6790\u548c\u7814\u7a76\u4efb\u52a1\u90fd\u5f88\u91cd\uff0c\u56e0\u6b64\u6211\u4eec\u5fc5\u987b\u5f00\u53d1\u65b0\u7684\u8f6f\u4ef6\u529f\u80fd\uff08\u4f8b\u5982\u6db2\u4f4d\u7279\u5b9a\u79fb\u6db2\uff09\u548c\u786c\u4ef6\u529f\u80fd\uff08\u4f8b\u5982\u6539\u8fdb\u7684\u78c1\u73e0\u5206\u79bb\u7cfb\u7edf\uff09\uff0c\u5b83\u4f7f\u6211\u4eec\u80fd\u591f\u76f4\u89c2\u5730\u5ba1\u67e5\u534f\u8bae\u4e2d\u7684\u6240\u6709\u6b65\u9aa4\uff0c\u4ee5\u53d1\u73b0\u9519\u8bef\u5e76\u9a8c\u8bc1\u8d28\u91cf\u3002<\/p>\n\n\n\n<p><strong>Q: <strong>\u60a8\u5728 <strong><a href=\"https:\/\/github.com\/retrobiosciences\/opentrons-10x\" target=\"_blank\" rel=\"noreferrer noopener\">Github<\/a>\u00a0<\/strong>\u4e0a\u53d1\u8868\u4e86\u8fd9\u9879\u5de5\u4f5c\uff0c\u5f15\u53d1\u4e86\u4e0d\u9519\u7684\u53cd\u54cd\uff0c \u60a8\u4e3a\u4ec0\u4e48\u51b3\u5b9a\u516c\u5f00\u8fd9\u9879\u5de5\u4f5c\uff1f<\/strong><\/strong><br>A:<strong>\u00a0<\/strong>\u5bf9\u6211\u4eec\u6765\u8bf4\uff0c\u5728\u5e7f\u6cdb\u7684\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u964d\u4f4e\u79d1\u7814\u6210\u672c\u5bf9\u793e\u4f1a\u7684\u597d\u5904\uff0c\u8fdc\u8fdc\u8d85\u8fc7\u4e86\u6211\u4eec\u5bf9\u540c\u884c\u7684\u7ade\u4e89\u5173\u7cfb\u7684\u72ed\u9698\u62c5\u5fe7\u3002\u6211\u4eec\u5e0c\u671b\u6709\u66f4\u591a\u4ece\u4e8b\u540c\u7c7b\u7814\u7a76\u7684\u79d1\u7814\u8005\u53ef\u4ee5\u4ece\u81ea\u52a8\u5316\u4e2d\u83b7\u76ca\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>Retro Biosciences \u901a\u8fc7\u5c55\u793aOpentrons\u5f00\u6e90\u81ea\u52a8\u5316\u79fb\u6db2\u7cfb\u7edf\u521b\u9020\u4e86\u5c5e\u4e8e\u81ea\u5df1\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u6587\u5e93\u5236\u5907\u81ea\u52a8\u5316\u6d41\u7a0b\uff0c\u5e2e\u52a9\u4e86\u66f4\u591a\u7814\u7a76\u5355\u7ec6\u80de\u6d4b\u5e8f\u7684\u5b9e\u9a8c\u5ba4\u80fd\u591f\u542f\u52a8\u81ea\u52a8\u5316\uff0c\u63d0\u5347\u4ed6\u4eec\u7684\u7814\u7a76\u6548\u7387\u3002\u6211\u4eec\u7684\u5f00\u6e90\u81ea\u52a8\u5316\u7a0b\u5e8f\u53ef\u4ee5\u901a\u8fc7\u7f16\u7a0b\u6765\u5b8c\u6210\u7814\u7a76\u4eba\u5458\u9700\u8981\u7684\u4efb\u4f55\u4e8b\u60c5\uff0c\u540c\u65f6\u628a\u6210\u672c\u63a7\u5236\u5728\u8f83\u4f4e\u7684\u8303\u56f4\u5185\u3002\u6211\u4eec\u88ab Retro Biosciences \u5f15\u9886\u7684\u521b\u65b0\u6240\u9707\u64bc\uff0c\u671f\u5f85\u4ed6\u4eec\u672a\u6765\u7684\u6301\u7eed\u521b\u65b0\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5355\u7ec6\u80de RNA \u6d4b\u5e8f\u80fd\u591f\u4ee5\u5355\u7ec6\u80de\u4e3a\u5355\u4f4d\u8fdb\u884c\u8f6c\u5f55\u7ec4\u5b66\u5206\u6790\uff0c\u8fd9\u9879\u7814\u7a76\u53ef\u4ee5\u8ba9\u6211\u4eec\u8fdb\u4e00\u6b65\u4e86\u89e3\u4e00\u4e9b\u7a00\u6709\u4e14\u8d35\u91cd\u7684\u7ec6\u80de\u7fa4\u3002<\/p>\n","protected":false},"featured_media":517,"template":"","class_list":["post-516","case","type-case","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Retro Biosciences\u4f7f\u7528OT-2\u81ea\u52a8\u5316\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6587\u5e93\u5236\u5907 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/case\/retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2\/\" \/>\n<meta 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