{"id":557,"date":"2020-01-07T14:06:00","date_gmt":"2020-01-07T06:06:00","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=case&p=557"},"modified":"2023-12-29T16:58:26","modified_gmt":"2023-12-29T08:58:26","slug":"assembling-synthetic-dna-easily-and-cheaply-at-imperial-college","status":"publish","type":"case","link":"https:\/\/opentrons.com.cn\/resources\/customer-stories\/assembling-synthetic-dna-easily-and-cheaply-at-imperial-college\/","title":{"rendered":"\u5e1d\u56fd\u7406\u5de5\u5b66\u9662\u4f7f\u7528 OT-2 \u6784\u5efa\u81ea\u52a8\u5316DNA\u7ec4\u88c5\u5e73\u53f0"},"content":{"rendered":"\n

\u5982\u4f55\u5229\u7528BASIC\u548cOpentrons OT-2\u6784\u5efa\u4e86\u4e00\u4e2a\u7528\u4e8e\u5408\u6210\u751f\u7269\u5b66\u7684\u51c6\u786e\u3001\u9ad8\u6027\u4ef7\u6bd4\u3001\u81ea\u52a8\u5316\u7684DNA\u7ec4\u88c5\u5e73\u53f0\u3002<\/h2>\n\n\n\n

\u4e3a\u4e86\u66f4\u6df1\u5165\u3001\u66f4\u5feb\u901f\u5730\u63a2\u7d22\u751f\u7269\u5b66\uff0c\u79d1\u5b66\u5bb6\u4eec\u9700\u8981\u7b80\u5355\u6709\u6548\u7684\u65b9\u6cd5\u6765\u6784\u5efa DNA \u7ed3\u6784\u3002<\/p>\n\n\n\n

\u201c\u4e3a\u4e86\u66f4\u6709\u6548\u5730\u5f00\u53d1\u65b0\u7684\u751f\u7269\u7cfb\u7edf\uff0c\u6211\u4eec\u6b63\u5728\u5c1d\u8bd5\u5c06\u5de5\u7a0b\u65b9\u6cd5\u5e94\u7528\u4e8e\u751f\u7269\u5b66\u3002\u201d\u5e1d\u56fd\u7406\u5de5\u5b66\u9662\u5408\u6210\u751f\u7269\u5b66\u4e2d\u5fc3<\/a>\u7684\u8054\u5408\u4e3b\u4efb Geoff Baldwin<\/a> \u8bf4\uff1a\u201c\u4ece\u8fc7\u5f80\u7684\u7ecf\u9a8c\u6765\u770b\uff0c\u5236\u9020\u65b0\u7684 DNA \u7ed3\u6784\u662f\u4e2a\u6f2b\u957f\u7684\u8fc7\u7a0b\uff0c\u901a\u5e38\u9700\u8981\u4e24\u5230\u4e09\u4e2a\u6708\u7684\u65f6\u95f4\u2014\u2014\u800c\u4e14\u4e2d\u9014\u6709\u53ef\u80fd\u5931\u8d25\u3002\u201d \u4e8e\u662f Baldwin \u548c\u4ed6\u7684\u540c\u4e8b Marko Storch<\/a> \u4ee5\u53ca\u4f26\u6566\u751f\u7269\u94f8\u9020\u5382<\/a>\u81ea\u52a8\u5316\u4e3b\u7ba1\u535a\u58eb\u540e\u7814\u7a76\u5458 Matt Haines <\/a>\u4e00\u8d77\u5f00\u53d1\u4e00\u6b3e\u53ef\u4ee5\u66f4\u8f7b\u677e\u3001\u66f4\u5feb\u901f\u6784\u5efaDNA\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\uff08\u4ece\u5de6\u5230\u53f3\uff09Matt Haines\u548cGeoff Baldwin\u5728\u4ed6\u4eec\u7684\u5b9e\u9a8c\u5ba4\u91cc\uff0c\u4e0eOT-2\u6db2\u4f53\u5904\u7406\u5b9e\u9a8c\u5ba4\u673a\u5668\u4eba\u5728\u4e00\u8d77\u3002\u56fe\u7247\u6765\u6e90\uff1a\u5e1d\u56fd\u7406\u5de5\u5b66\u9662<\/p>\n\n\n\n

\u6211\u4eec\u5df2\u7ecf\u505a\u51fa\u4e86\u4e00\u4e9b\u5de5\u5177\u4e0a\u7684\u6539\u8fdb\u3002\u201c\u4eca\u5929\uff0c\u6211\u4eec\u6709\u66f4\u597d\u7684\u5de5\u5177\uff0c\u201dBaldwin\u8bf4\uff0c\u201c\u5c31\u50cf\u4f60\u5efa\u9020\u5b9c\u5bb6\u5bb6\u5177\u7684\u65b9\u5f0f\u4e00\u6837\uff0c\u4f60\u53ef\u4ee5\u7528\u6807\u51c6\u96f6\u4ef6\u5efa\u9020\u4e00\u4e2a\u751f\u7269\u7cfb\u7edf\uff0c\u8fd9\u662f\u4e00\u79cd\u66f4\u6709\u6548\u7684\u65b9\u5f0f\u3002\u201d<\/p>\n\n\n\n

\n

\u201c\u5c31\u50cf\u4f60\u5efa\u9020\u5b9c\u5bb6\u5bb6\u5177\u7684\u65b9\u5f0f\u4e00\u6837\uff0c\u4f60\u53ef\u4ee5\u7528\u6807\u51c6\u96f6\u4ef6\u5efa\u9020\u4e00\u4e2a\u751f\u7269\u7cfb\u7edf\uff0c\u8fd9\u662f\u4e00\u79cd\u66f4\u6709\u6548\u7684\u65b9\u5f0f\u3002\u201d<\/p>\n<\/blockquote>\n\n\n\n

\u8fdb\u5165\u57fa\u7840\u77e5\u8bc6<\/strong>
\u5728\u5f00\u542f\u81ea\u52a8\u5316\u9879\u76ee\u4e4b\u524d\uff0cStorch\u3001Baldwin \u548c\u5176\u4ed6\u540c\u4e8b\u5f00\u53d1\u4e86 BASIC\uff0c\u4e3b\u8981\u7528\u4e8e\u5e42\u7b49\u514b\u9686\u7684\u751f\u7269\u90e8\u4ef6\u7ec4\u88c5\u6807\u51c6\u5316\u3002\u5728\u67d0\u79cd\u7a0b\u5ea6\u4e0a\uff0cBASIC \u4fc3\u4f7f\u7814\u7a76\u4eba\u5458\u4ee5\u65b0\u7684\u65b9\u5f0f\u601d\u8003\u6784\u5efa DNA \u7ed3\u6784\u3002\u201c\u5b83\u63d0\u4f9b\u4e86\u51fa\u8272\u7684\u53ef\u64cd\u4f5c\u6027\uff0c\u201dBaldwin\u8bf4\uff1a\u201c\u4e00\u65e6\u4f60\u63a5\u53d7\u4e86\u6807\u51c6\u5316\u65b9\u6cd5\uff0c\u5c31\u4f1a\u6709\u5927\u7a0b\u5ea6\u7684\u601d\u8003\u81ea\u7531\uff0c\u4f60\u4e0d\u518d\u9700\u8981\u8003\u8651\u5355\u4e2a\u5e8f\u5217\u3001\u91cd\u53e0\u3001PCR \u7b49\u7684\u7ec6\u8282\u3002\u201d<\/p>\n\n\n\n

\u5728\u67d0\u79cd\u7a0b\u5ea6\u4e0a\uff0cBASIC \u4fc3\u4f7f\u7814\u7a76\u4eba\u5458\u4ee5\u65b0\u7684\u65b9\u5f0f\u601d\u8003\u6784\u5efaDNA\u6784\u5efa\u4f53\u3002\u201c\u5b83\u63d0\u4f9b\u4e86\u6781\u597d\u7684\u53ef\u64cd\u4f5c\u6027\uff0c\u201dBaldwin\u8bf4\u3002\u201c\u4e00\u65e6\u4f60\u63a5\u53d7\u4e86\u6807\u51c6\u5316\u7684\u65b9\u6cd5\uff0c\u5c31\u4f1a\u6709\u66f4\u5927\u7684\u81ea\u8003\u81ea\u7531\u5ea6\u2014\u2014\u4f60\u4e0d\u518d\u9700\u8981\u8003\u8651\u5355\u4e2a\u5e8f\u5217\u3001\u91cd\u53e0\u3001PCR\u7b49\u7684\u7ec6\u8282\u3002\u201d<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u901a\u8fc7 BASIC\uff0c\u79d1\u5b66\u5bb6\u4eec\u5c06\u6ce8\u610f\u529b\u4ece\u5982\u4f55\u6784\u5efa DNA \u8f6c\u79fb\u5230\u4e86\u601d\u8003\u7cfb\u7edf\u7684\u884c\u4e3a\u4e0a\u3002\u6b64\u5916\uff0cBASIC \u4ea7\u751f\u7684DNA \u6784\u5efa\u975e\u5e38\u51c6\u786e\u3002\u201c\u4f60\u6709\u4fe1\u5fc3\uff0c\u5408\u6210\u7684 DNA\u5c31\u662f\u4f60\u60f3\u8981\u7684\uff0c\u201dBaldwin\u8bf4\u9053\u3002<\/p>\n\n\n\n

\n

\u901a\u8fc7 BASIC\uff0c\u79d1\u5b66\u5bb6\u4eec\u5c06\u6ce8\u610f\u529b\u4ece\u5982\u4f55\u6784\u5efa DNA \u8f6c\u79fb\u5230\u4e86\u601d\u8003\u7cfb\u7edf\u7684\u884c\u4e3a\u4e0a\uff0c\u540c\u65f6\u751f\u6210\u975e\u5e38\u51c6\u786e\u7684DNA\u6784\u5efa\u3002<\/p>\n<\/blockquote>\n\n\n\n

\u9664\u4e86\u63d0\u9ad8\u51c6\u786e\u6027\u5916\uff0cBASIC \u8fd8\u7b80\u5316\u4e86\u6784\u5efa DNA \u7684\u8fc7\u7a0b\u3002\u201c\u5b83\u63d0\u4f9b\u4e86\u66f4\u7b80\u5355\u7684\u65b9\u6cd5\u6765\u5b9e\u73b0 DNA \u6784\u5efa\uff0c\u201dStorch\u8bf4\u3002\u201c\u4f60\u5f97\u5230\u7684\u662f\u5f00\u7bb1\u5373\u7528\u7684\u96f6\u4ef6\u548c\u8fde\u63a5\u5668\uff0c\u800c\u4e14\u4e0d\u9700\u8981\u8fdb\u884c\u6545\u969c\u6392\u9664\u3002\u201d\u7136\u540e\uff0c\u79d1\u5b66\u5bb6\u53ea\u9700\u8981\u77e5\u9053\u5982\u4f55\u5c06\u90e8\u5206-\u8fde\u63a5\u5668-\u90e8\u5206-\u8fde\u63a5\u5668\u7b49\u8fdb\u884c\u7ec4\u5408\u6765\u6784\u5efa\u4e00\u4e2a\u7ed3\u6784\u3002<\/p>\n\n\n\n

Baldwin \u5c06\u5176\u4e0e\u8ba1\u7b97\u673a\u7684\u8fdb\u5316<\/a>\u8fdb\u884c\u6bd4\u8f83\u3002\u51e0\u5341\u5e74\u524d\uff0c\u6784\u5efa\u8ba1\u7b97\u673a\u7684\u4eba\u9700\u8981\u4e86\u89e3\u6676\u4f53\u7ba1\u548c\u903b\u8f91\u95e8\u3002\u201c\u5982\u4eca\uff0c\u60a8\u4e0d\u5fc5\u62c5\u5fc3\u8ba1\u7b97\u7684\u7269\u7406\u539f\u7406\uff0c\u56e0\u4e3a\u60a8\u53ef\u4ee5\u76f4\u63a5\u4ece\u76d2\u5b50\u91cc\u62ff\u51fa\u7ec4\u4ef6\uff0c\u201d\u4ed6\u8bf4\u3002\u201c\u540c\u6837\uff0cBASIC \u8ba9\u7528\u6237\u80fd\u591f\u4eab\u53d7\u4e0eDNA \u7c7b\u4f3c\u7684\u62bd\u8c61\u5c42\u6b21\u3002\u201d<\/p>\n\n\n\n

\u4e0d\u8fc7\uff0c\u60f3\u8981\u5145\u5206\u5229\u7528 BASIC\uff0c\u8fd8\u9700\u8981\u5b9e\u9a8c\u5ba4\u81ea\u52a8\u5316\u3002<\/p>\n\n\n\n

\u6dfb\u52a0 Opentrons<\/strong>
\u201c\u5f53 Opentrons \u63a8\u51faOT-2\u65f6\uff0cOpentrons\u7684\u5f00\u6e90\u7406\u5ff5\u786e\u5b9e\u4e0e\u6211\u4eec\u5bf9\u57fa\u4e8e\u94fe\u63a5\u5668\u7684 DNA \u7ec4\u88c5\u9879\u76ee\u7684\u770b\u6cd5\u4e0d\u8c0b\u800c\u5408\uff0c\u201dStorch \u8bf4\uff1a\u201c\u78c1\u6027\u6a21\u5757\u548c\u6e29\u63a7\u6a21\u5757\u63d0\u4f9b\u4e86\u6211\u4eec\u9700\u8981\u7684\u529f\u80fd\u3002\u201d<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u5f00\u6e90 BASIC \u6280\u672f\uff08\u53ef\u5728 GitHub \u4e0a\u514d\u8d39\u83b7\u5f97<\/a>\uff09\u975e\u5e38\u9002\u5408\u5728\u5f00\u6e90\u7684\u81ea\u52a8\u5316\u79fb\u6db2\u673a\u5668\u4ebaOT-2\u4e0a\u4f7f\u7528\u3002\u201c\u6211\u4eec\u76f8\u4fe1\u5b9e\u9a8c\u7684\u4ef7\u503c\u5e94\u8be5\u4f53\u73b0\u5728\u4f60\u5efa\u9020\u7684\u4e1c\u897f\u4e0a\uff0c\u800c\u4e0d\u662f\u4f60\u5efa\u9020\u5b83\u7684\u65b9\u5f0f\u4e0a\u201dBaldwin\u8bf4\u3002\u8fd9\u50ac\u751f\u4e86\u80fd\u591f\u8fd0\u884c BASIC \u7684 OT-2\u2014\u2014DNA-BOT\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\uff08\u4ece\u5de6\u81f3\u53f3\uff09Matt Haines\u548cGeoff Baldwin\u6b63\u5728\u601d\u8003\u4ed6\u4eec\u7684DNA-Bot\u3002\u56fe\u7247\u6765\u6e90\uff1a\u5e1d\u56fd\u7406\u5de5\u5b66\u9662<\/p>\n\n\n\n

\u5f53\u88ab\u95ee\u53ca\u8fd0\u884c DNA-BOT \u7684\u4f18\u52bf\u65f6\uff0cHaines \u8bf4\uff0c\u201c\u9996\u5148\u662f\u6027\u4ef7\u6bd4\u3002\u201d \u7136\u540e\uff0c\u4ed6\u8fd8\u5f3a\u8c03\u4e86\u8be5\u65b9\u6cd5\u7684\u6548\u7387\u548c\u51c6\u786e\u6027\u3002\u201c\u6211\u4eec\u5728\u6bcf\u4e2a\u6848\u4f8b\u4e2d\u90fd\u83b7\u5f97\u4e86 88 \u4e2a\u7ec4\u4ef6\u7684\u6784\u9020\uff0c\u518d\u9ad8\u6548\u7684\u540c\u65f6\u5b9e\u73b0\u4e86\u9ad8\u7cbe\u5ea6\uff0c\u201d\u4ed6\u8bf4\uff1a\u201c\u8fd9\u662f\u975e\u6807\u51c6\u5316\u7684\u65b9\u5f0f\u96be\u4ee5\u5b9e\u73b0\u7684\u3002\u201d<\/p>\n\n\n\n

\u5728\u5e1d\u56fd\u7406\u5de5\u5b66\u9662\u5408\u6210\u751f\u7269\u5b66\u4e2d\u5fc3\uff0cBaldwin \u548c\u4ed6\u7684\u540c\u4e8b\u5efa\u7acb\u4e86\u4e00\u4e2a\u7531\u516d\u4e2a DNA-BOT \u7ec4\u6210\u7684\u5b9e\u9a8c\u5ba4\u3002\u201c\u6211\u4eec\u6b63\u5728\u5c1d\u8bd5\u770b\u770b\u7855\u58eb\u751f\u662f\u5426\u53ef\u4ee5\u4f7f\u7528\u8fd9\u4e2a\u5e73\u53f0\u6784\u5efa\u7ed3\u6784\uff0c\u201dStorch \u8bf4\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u7814\u7a76\u751f\u6b63\u5728\u8bbe\u7f6e\u5e1d\u56fd\u7406\u5de5\u5b66\u9662\u751f\u7269\u94f8\u9020\u5382\u3002\u56fe\u7247\u6765\u6e90\uff1a\u4f26\u6566\u5e1d\u56fd\u5b66\u9662<\/em><\/p>\n\n\n\n

\u65e0\u8bba\u8fd9\u4e9b\u5b66\u751f\u7684\u8bd5\u9a8c\u7ed3\u679c\u5982\u4f55\uff0cBaldwin \u7684\u56e2\u961f\u5df2\u7ecf\u77e5\u9053 DNA-BOT \u6539\u53d8\u4e86\u6e38\u620f\u89c4\u5219\u3002\u201c\u6211\u4eec\u53ef\u4ee5\u63a2\u7d22\u7684\u8bbe\u8ba1\u7a7a\u95f4\u662f\u6570\u767e\u4e2a\uff0c\u751a\u81f3\u6570\u5343\u4e2aDNA\u7ed3\u6784\uff0c\u201dBaldwin \u8bf4\uff0c\u201c\u800c\u4e14\u5b83\u53ef\u4ee5\u8fc5\u901f\u589e\u52a0\u5230\u6570\u4e07\u4e2a\u3002\u201d<\/p>\n\n\n\n

\u6b63\u5982 Haines \u6240\u5f3a\u8c03\u7684\uff0c\u201c\u8fd8\u6709\u5f88\u591a\u53ef\u63a2\u7d22\u7684\u53d8\u5f02\u6027\u3002\u201d<\/p>\n\n\n\n

\u589e\u5f3a\u5e94\u7528<\/strong>
Baldwin \u7684\u56e2\u961f\u770b\u5230\u4e86\u66f4\u591a\u7684\u5de5\u4f5c\u8981\u505a\u3002\u4ed6\u4eec\u5df2\u7ecf\u770b\u5230 DNA-BOT \u5728\u5404\u79cd\u5e94\u7528\u4e2d\u53d1\u6325\u4f5c\u7528\uff0c\u5305\u62ec\u7814\u7a76\u73b0\u6709\u7684\u751f\u7269\u5408\u6210\u9014\u5f84\u548c\u521b\u5efa\u65b0\u7684\u9014\u5f84\u6765\u6784\u5efa\u96be\u4ee5\u5236\u9020\u7684\u5316\u5408\u7269\u3002<\/p>\n\n\n\n

\u8be5\u56e2\u961f\u5c06\u7ee7\u7eed\u5f00\u53d1\u548c\u8c03\u6574 DNA-BOT\uff0c\u5e76\u8ba1\u5212\u6574\u5408 Opentrons Thermocycler\u3002 \u201c\u6211\u4eec\u60f3\u5efa\u7acb\u4e00\u4e2a\u751f\u6001\u7cfb\u7edf\uff0c\u201dBaldwin \u8bf4\u3002\u201c\u521b\u5efa\u4e00\u4e2a DNA \u96f6\u4ef6\u548c\u6d41\u7a0b\uff0c\u5c06\u589e\u5f3a\u56e2\u961f\u6210\u5458\u7684\u80fd\u529b\u3002\u201d\u5f53\u4ef7\u683c\u5b9e\u60e0\u3001\u6613\u4e8e\u4f7f\u7528\u548c\u9ad8\u6548\u7684\u81ea\u52a8\u5316\u4e0e\u5148\u8fdb\u7684\u5408\u6210\u751f\u7269\u5b66\u5de5\u5177\u7bb1\u76f8\u7ed3\u5408\u65f6\uff0c\u6784\u5efa DNA \u7ed3\u6784\u7684\u6574\u4e2a\u884c\u4e1a\u5c06\u53d1\u751f\u53d8\u5316\uff0c\u51e0\u4e4e\u4efb\u4f55\u4eba\u90fd\u53ef\u4ee5\u63a2\u7d22\u5b83\u3002<\/p>\n\n\n\n

\u53c2\u8003\u8d44\u6599\uff1a<\/strong><\/p>\n\n\n\n

    \n
  1. Storch, M., Haines, M.C., Baldwin, G.S. 2019. DNA-BOT: \u5408\u6210\u751f\u7269\u5b66\u4e2d\u4f4e\u6210\u672c\u3001\u81ea\u52a8\u5316\u7684DNA\u7ec4\u88c5\u5e73\u53f0\u3002bioRxiv. doi:\u00a0http:\/\/dx.doi.org\/10.1101\/832139<\/a>.<\/li>\n\n\n\n
  2. Storch, M., Casini, A., Mackrow, B., et al. 2015. BASIC: \u7528\u4e8e\u5408\u6210\u751f\u7269\u5b66\u7684\u540c\u6001\u514b\u9686\u7684\u65b0\u7684\u751f\u7269\u90e8\u4ef6\u7ec4\u88c5\u6807\u51c6\uff0c\u4e3a\u5408\u6210\u751f\u7269\u5b66\u63d0\u4f9b\u51c6\u786e\u3001\u5355\u5c42DNA\u7ec4\u88c5\uff0c ACS Synth. Biol. 4:781\u2013787.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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