{"id":2751,"date":"2024-05-27T14:40:56","date_gmt":"2024-05-27T06:40:56","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2751"},"modified":"2024-09-03T17:48:49","modified_gmt":"2024-09-03T09:48:49","slug":"opentrons-flex","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/opentrons-flex\/","title":{"rendered":"Opentrons Flex \u81ea\u52a8\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad924 \u5c0f\u65f6\u5168\u7a0b\u81ea\u52a8\u5316\u8f7b\u677e\u5236\u5907\u9ad8\u8fbe 384 \u4e2a\u8d28\u7c92\u6587\u5e93"},"content":{"rendered":"\n<p style=\"font-size:16px\"><strong>\u7b80\u4ecb<\/strong><br>\u9ad8\u901a\u91cf\u57fa\u56e0\u7ec4\u5206\u6790\u662f\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u91cd\u8981\u5de5\u5177\uff0c\u8fd9\u9879\u6280\u672f\u5728\u63a8\u52a8\u79d1\u7814\u8fdb\u6b65\u65b9\u9762\u53d1\u6325\u7740<br>\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4e3a\u4e86\u6ee1\u8db3\u4e86\u79d1\u7814\u9886\u57df\u5bf9\u9ad8\u6548\u3001\u51c6\u786e\u5236\u5907\u65b9\u6cd5\u7684\u8feb\u5207\u9700\u6c42\uff0cseqWell \u7684 Express Plex\u2122<br>Library Kit \u63d0\u4f9b\u4e86\u4e00\u79cd\u4e13\u95e8\u7528\u4e8e\u9ad8\u901a\u91cf\u5236\u5907\u8d28\u7c92\u548c\u6269\u589e\u5b50\u6587\u5e93\u7684\u89e3\u51b3\u65b9\u6848\u3002<br>\u5728\u4f20\u7edf\u7684\u624b\u5de5\u5236\u5907\u6587\u5e93\u65b9\u6cd5\u4e2d\uff0c\u5b8c\u6210 96 \u4e2a\u6837\u54c1\u7684\u5236\u5907\u9700\u8981\u8017\u8d39\u957f\u8fbe 90 \u5206\u949f\u7684\u65f6\u95f4\uff0c\u5176\u4e2d\u624b\u52a8\u64cd<br>\u4f5c\u65f6\u95f4\u5360\u636e\u4e86 30 \u5206\u949f\u3002\u8fd9\u79cd\u65b9\u5f0f\u4e0d\u4ec5\u6548\u7387\u4f4e\u4e0b\uff0c\u8fd8\u5bb9\u6613\u56e0\u4e3a\u4eba\u4e3a\u56e0\u7d20\u5f15\u5165\u8bef\u5dee\u3002<br>\u4e3a\u4e86\u6539\u8fdb\u8fd9\u4e00\u72b6\u51b5\uff0c\u672c\u6587\u5bf9\u6bd4\u4e86\u4e24\u79cd\u6587\u5e93\u5236\u5907\u65b9\u6cd5\uff0c\u4e00\u79cd\u662f\u5728 Opentrons Flex\u2122 \u79fb\u6db2\u5de5\u4f5c\u7ad9\u4e0a\u4f7f\u7528<br>seqWell ExpressPlex\u2122 Library Kit \uff0c\u53e6\u4e00\u79cd\u662f\u624b\u5de5\u5236\u5907\u6587\u5e93\u3002<br>\u901a\u8fc7\u7ed3\u5408 Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u4e0e seqWell ExpressPlex\u2122 Library Kit\uff0c\u6211\u4eec\u53ef\u4ee5\u663e\u8457\u7f29\u77ed\u6587\u5e93\u5236\u5907\u65f6<br>\u95f4\uff0c\u63d0\u9ad8\u6837\u672c\u901a\u91cf\u3002\u8fd9\u79cd\u81ea\u52a8\u5316\u7684\u89e3\u51b3\u65b9\u6848\u4e0d\u4ec5\u63d0\u5347\u4e86\u5236\u5907\u6548\u7387\uff0c\u8fd8\u964d\u4f4e\u4e86\u4eba\u4e3a\u8bef\u5dee\u7684\u98ce\u9669\uff0c\u4e3a\u751f\u547d\u79d1\u5b66<br>\u7814\u7a76\u63d0\u4f9b\u4e86\u66f4\u4e3a\u53ef\u9760\u3001\u9ad8\u6548\u7684\u5de5\u5177\u3002<br><strong>\u6750\u6599\u4e0e\u65b9\u6cd5<\/strong><br>ExpressPlex \u5de5\u4f5c\u6d41\u7a0b:<br>\u6839\u636e Express Plex \u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u6211\u4eec\u5728 Flex \u81ea\u52a8\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad9\u4e0a\u8fdb\u884c\u6587\u5e93\u5236\u5907\u3002\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\uff0c\u6211\u4eec\u8f93\u5165<br>16 ng \u7684 pUC19 \uff0c\u518d\u5c06\u6240\u6709\u7ec4\u5206\u52a0\u5165\u53cd\u5e94\u677f \uff0c \u7528 3000 rpm \u7684\u8f6c\u901f\u6df7\u5300 90 \u79d2\uff0c\u7136\u540e\u8fdb\u884c\u540e\u7eed\u7684\u6587\u5e93\u5236<br>\u5907\u548c\u6df7\u5408\u6b65\u9aa4\u3002\u6700\u7ec8\u7684\u6587\u5e93\u5728 NextSeq 2000 \u6d4b\u5e8f\u4eea\u4e0a\u8fdb\u884c 2X150 bp \u6d4b\u5e8f\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"623\" height=\"1024\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898-623x1024.png\" alt=\"ExpressPlex \u5de5\u4f5c\u6d41\u7a0b\" class=\"wp-image-2569\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898-623x1024.png 623w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898-183x300.png 183w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898-768x1262.png 768w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898-351x576.png 351w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898.png 900w\" sizes=\"(max-width: 623px) 100vw, 623px\" \/><\/figure>\n\n\n\n<p>\u56fe 1.<br>A ) Express Plex \u5de5\u4f5c\u6d41\u7a0b\u5305\u62ec\u5c06 index \u548c DNA \u52a0\u5165 \u53cd\u5e94\u677f\uff08\u5df2\u542b\u6709\u9176\u7b49\u6df7\u5408\u7269\uff09\uff0c\u7136\u540e\u8fdb\u884c\u6269\u589e\u3001\u6df7\u5408<br>\u548c MAGwise \u78c1\u73e0\u7eaf\u5316\uff1b<br>B ) Flex \u5de5\u4f5c\u7ad9\u5305\u62ec\u5438\u5934\u76d2\u3001\u6a21\u5757\u3001\u8f6c\u677f\u6293\u624b\u548c\u5176\u4ed6\u914d\u4ef6\uff1b<br>C ) Flex \u5de5\u4f5c\u7ad9\u677f\u4f4d\u8bbe\u7f6e\uff0c\u7528\u4e8e\u5728\u6269\u589e\u540e\u5c06 4 \u4e2a\u53cd\u5e94\u677f\u8fdb\u884c\u6587\u5e93\u6df7\u5408\u3002<\/p>\n\n\n\n<p><strong>\u7ed3\u679c<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"512\" height=\"499\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/0012.jpg\" alt=\"Opentrons Flex\u663e\u793a\u7ed3\u679c\" class=\"wp-image-2570\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/0012.jpg 512w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/0012-300x292.jpg 300w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/><\/figure>\n\n\n\n<p>\u56fe 2 . A ) \u7ed3\u679c\u663e\u793a 96 \u5b54\u677f\u4e2d\u6587\u5e93\u6570\u636e\u53d8\u5f02\u7cfb\u6570( CV )\u4e3a 12.7%\uff1b B ) 95% \u7684\u6587\u5e93\u6570\u636e\u901a\u8fc7\u4e86\u6570\u636e\u9884\u5904<br>\u7406\uff0c\u4e3a\u9ad8\u8d28\u91cf\u6570\u636e\uff0c\u4e14\u8d85\u8fc7 90% \u6587\u5e93\u8d28\u91cf\u5f97\u5206\u8fbe\u5230 Q30\uff1b C ) \u7531 Flex \u5236\u5907\u7684\u6587\u5e93\u6bd4\u624b\u52a8\u5236\u5907\u7684\u6587\u5e93\u6d53\u5ea6<br>\u66f4\u9ad8\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"793\" height=\"225\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/00120240513201156.jpg\" alt=\"\u72ec\u7acb\u7684 96 \u6df7 1 \u6587\u5e93\" class=\"wp-image-2571\" style=\"width:840px;height:auto\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/00120240513201156.jpg 793w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/00120240513201156-300x85.jpg 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/00120240513201156-768x218.jpg 768w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/00120240513201156-576x163.jpg 576w\" sizes=\"(max-width: 793px) 100vw, 793px\" \/><\/figure>\n\n\n\n<p>\u56fe 3 . \u5229\u7528\u9519\u5f00\u7684\u5f00\u59cb\u65f6\u95f4\uff0c\u53ef\u4ee5\u5728 Opentrons FlexTM \u4e0a 3 \u5c0f\u65f6\u5185\u5236\u5907 8 \u4e2a\u72ec\u7acb\u7684 96 \u6df7 1 \u6587<br>\u5e93\u3002\u5728 Flex \u4e0a\u8bbe\u7f6e\u7b2c\u4e00\u5757\u53cd\u5e94\u677f\u540e\uff0c\u53ef\u4ee5\u5728 24 \u5c0f\u65f6\u5185\u5b8c\u6210 768 \u4e2a\u6587\u5e93\u7684\u5236\u5907\u3002<\/p>\n\n\n\n<p><strong>\u7ed3\u8bba<\/strong><br>\u5728 Flex \u79fb\u6db2\u5e73\u53f0\u4e0a\u5236\u5907\u7684 96 \u4e2a pUC19 \u8d28\u7c92\u6837\u672c\u6587\u5e93\u5448\u73b0\u51fa\u4e86\u9ad8\u5ea6\u5747\u4e00\u7684\u8d28\u91cf\uff0c\u5145\u5206\u8bc1\u660e\u4e86 Flex<br>\u5e73\u53f0\u5728\u81ea\u52a8\u5316\u6587\u5e93\u5236\u5907\u65b9\u9762\u7684\u5353\u8d8a\u6027\u80fd\u3002<br>\u4e0e\u624b\u52a8\u5236\u5907\u76f8\u6bd4\uff0c\u81ea\u52a8\u5316\u6587\u5e93\u5236\u5907\u4e0d\u4ec5\u7a33\u5b9a\u6027\u66f4\u4f73\uff0c\u800c\u4e14\u6587\u5e93\u6d53\u5ea6\u66f4\u9ad8\u3002\u6b64\u5916\uff0c\u8d85\u8fc795%\u7684\u8bfb\u6570\u6210<br>\u529f\u901a\u8fc7\u6d4b\u5e8f\u4eea\u8d28\u68c0 PF\uff0c\u8fd9\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u4e86\u81ea\u52a8\u5316\u5236\u5907\u6d41\u7a0b\u7684\u9ad8\u6548\u6027\u548c\u51c6\u786e\u6027\u3002<br>Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u652f\u6301\u5728\u5355\u6b21\u8fd0\u884c\u4e2d\u5904\u7406\u591a\u8fbe 384 \u4e2a\u6837\u672c\uff0c\u6781\u5927\u5730\u63d0\u9ad8\u4e86\u6837\u672c\u5904\u7406\u6548\u7387\u3002\u901a\u8fc7\u5408\u7406<br>\u5b89\u6392\u65f6\u95f4\uff0c\u6211\u4eec\u751a\u81f3\u53ef\u4ee5\u5728 24 \u5c0f\u65f6\u5185\u5904\u7406\u9ad8\u8fbe 768 \u4e2a\u6837\u672c\uff0c\u4ece\u800c\u6ee1\u8db3\u5927\u89c4\u6a21\u9ad8\u901a\u91cf\u57fa\u56e0\u7ec4\u5206\u6790\u7684\u9700\u6c42\u3002<br>\u5728 Flex \u5e73\u53f0\u4e0a\u81ea\u52a8\u5316 ExpressPlex\u2122 Library Prep Kit \u5de5\u4f5c\u6d41\u7a0b\uff0c\u6211\u4eec\u5b9e\u73b0\u4e86\u4ece\u63d0\u53d6\u7684\u8d28\u7c92\/\u6269\u589e<br>\u5b50 DNA \u5230\u6570\u636e\u751f\u6210\u7684\u5168\u6d41\u7a0b\u81ea\u52a8\u5316\u3002\u8fd9\u4e00\u89e3\u51b3\u65b9\u6848\u4e0d\u4ec5\u7b80\u5316\u4e86\u64cd\u4f5c\u6b65\u9aa4\uff0c\u964d\u4f4e\u4e86\u4eba\u4e3a\u8bef\u5dee\uff0c\u800c\u4e14\u663e\u8457\u63d0<br>\u9ad8\u4e86\u5b9e\u9a8c\u6548\u7387\u548c\u6570\u636e\u8d28\u91cf\uff0c\u4e3a\u751f\u547d\u79d1\u5b66\u7814\u7a76\u9886\u57df\u7684\u53d1\u5c55\u6ce8\u5165\u4e86\u65b0\u7684\u52a8\u529b\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7b80\u4ecb\u9ad8\u901a\u91cf\u57fa\u56e0\u7ec4\u5206\u6790\u662f\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u91cd\u8981\u5de5\u5177\uff0c\u8fd9\u9879\u6280\u672f\u5728\u63a8\u52a8\u79d1\u7814\u8fdb\u6b65\u65b9\u9762\u53d1\u6325\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\u3002\u4e3a\u4e86\u6ee1 [&hellip;]<\/p>\n","protected":false},"featured_media":2568,"template":"","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"knowledge2_cat":[96],"knowledge2_app":[],"class_list":["post-2751","knowledge2","type-knowledge2","status-publish","has-post-thumbnail","hentry","knowledge2_cat-biji"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - 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