{"id":2768,"date":"2024-05-28T16:30:08","date_gmt":"2024-05-28T08:30:08","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2768"},"modified":"2024-09-03T17:44:36","modified_gmt":"2024-09-03T09:44:36","slug":"opentrons-flex-2","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/opentrons-flex-2\/","title":{"rendered":"\u4f7f\u7528 Opentrons Flex\u2122 \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\u7eaf\u5316\u4ece\u7ec6\u80de\u6216\u7ec4\u7ec7\u6837\u672c\u63d0\u53d6\u57fa\u56e0\u7ec4 DNA"},"content":{"rendered":"\n<p><strong>\u5e94\u7528\u4f18\u52bf<\/strong><\/p>\n\n\n\n<p>\u4f7f\u7528 Opentrons Flex\u2122 \u642d\u914dNucleoMag\u00ae Tissue \u8bd5\u5242\u76d2\uff0c\u8fdb\u884c\u7eaf\u5316\u6838\u9178\u7684\u4f18\u52bf\uff1a<\/p>\n\n\n\n<p class=\"has-text-align-left\">1.\u65b9\u6cd5\u7ecf\u8fc7\u9a8c\u8bc1\uff0c\u786e\u4fdd\u5168\u81ea\u52a8\u63d0\u53d6\u7eaf\u5316\u8fc7\u7a0b\u51c6\u786e\u6027\u3002<br>2.\u4ea7\u91cf\u548c\u7eaf\u5ea6\u91cd\u590d\u6027\u597d\uff0c\u7a33\u5b9a\u56de\u6536\u57fa\u56e0\u7ec4 DNA\u3002<br>3.\u5904\u7406\u80fd\u529b\u5f3a\uff0c\u53ef\u6700\u591a\u540c\u65f6\u5904\u7406 96\u4e2a\u6837\u54c1\u3002<br>4.\u53ef\u8bbf\u95ee Opentrons \u534f\u8bae\u5e93\u6216\u901a\u8fc7MACHEREY NAGEL \u7684\u81ea\u52a8\u5316\u6280\u672f\u652f\u6301automation-bio@mn-net.com\u83b7\u53d6\u66f4\u591a\u8be6\u7ec6\u4fe1\u606f\u3002<\/p>\n\n\n\n<p><strong>\u5bfc\u8a00<\/strong><\/p>\n\n\n\n<p>        \u63d0\u53d6\u57fa\u56e0\u7ec4 DNA \u662f\u751f\u547d\u79d1\u5b66\u548c\u5e94\u7528\u6d4b\u8bd5\u5b9e\u9a8c\u5ba4\u4e2d\u5e38\u89c1\u7684\u5b9e\u9a8c\u6d41\u7a0b\uff0c\u5c24\u5176\u5728\u57fa\u56e0\u5206\u578b\u6d4b\u8bd5\u3001\u6d4b\u5e8f\u548c\u4e34\u5e8a\u7814\u7a76\u7b49\u4e0b\u6e38\u5e94\u7528\u4e2d\uff0c\u5bf9 DNA \u7684\u8d28\u91cf\u548c\u7eaf\u51c0\u5ea6\u8981\u6c42\u4e25\u683c\u3002<br>        MACHEREY NAGEL \u7684 NucleoMag\u00ae Tissue \u8bd5\u5242\u76d2\u63d0\u4f9b\u4e86\u57fa\u4e8e\u78c1\u73e0\u7684\u5feb\u901f\u57fa\u56e0\u7ec4 DNA \u7eaf\u5316\u89e3\u51b3\u65b9\u6848\u3002\u8be5\u8bd5\u5242\u76d2\u8bbe\u8ba1\u7cbe\u7b80\uff0c\u9002\u7528\u4e8e 96 \u5b54\u677f\u6a21\u5f0f\uff0c\u53ef\u4e3a PCR\u3001NGS \u6216\u5176\u4ed6\u9176\u53cd\u5e94\u63d0\u4f9b\u9ad8\u8d28\u91cf\u3001\u9ad8\u7eaf\u51c0\u5ea6\u7684 DNA \u6a21\u677f\u3002<br>        \u7ed3\u5408 Opentrons Flex\u2122 \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\uff0c\u53ef\u9ad8\u6548\u7eaf\u5316 HeLa \u7ec6\u80de\u57fa\u56e0\u7ec4DNA\u3002\u8be5\u5de5\u4f5c\u7ad9\u914d\u5907Opentrons Flex\u2122 \u73af\u5f62\u78c1\u529b\u67b6\u3001\u52a0\u70ed\u632f\u8361\u6a21\u5757\uff0c\u4ee5\u53ca5-1000 \u00b5L \u5355\u901a\u9053\u548c 8 \u901a\u9053\u79fb\u6db2\u5668\uff0c\u8fd8\u53ef\u9009\u62e9 96 \u901a\u9053\u79fb\u6db2\u5668\uff0c\u9002\u7528\u4e8e\u5404\u79cd\u6837\u672c\u7c7b\u578b\u63d0\u53d6 DNA\uff0c\u5305\u62ec\u5176\u4ed6\u771f\u6838\u7ec6\u80de\u6216\u7ec4\u7ec7\u3002<br>        Opentrons Protocol Library \u4e2d\u63d0\u4f9b\u4e86\u7ed3\u5408 Opentrons Flex\u2122 \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\u4e0e NucleoMag\u00ae Tissue \u8bd5\u5242\u76d2\u4f7f\u7528\u7684\u534f\u8bae\uff0c\u53ef\u76f4\u63a5\u4e0b\u8f7d\u4f7f\u7528\u3002<\/p>\n\n\n\n<p><strong>\u5e94\u7528\u8bf4\u660e NucleoMag\u00ae \u7ec4\u7ec7\u4e0e Opentrons Flex<\/strong>\u2122<\/p>\n\n\n\n<p>         NucleoMag\u00ae Tissue \u8bd5\u5242\u76d2\u7684\u539f\u7406\u662f\u57fa\u4e8e\u6838\u9178\u5728\u7279\u5b9a\u7ed3\u5408\u6761\u4ef6\u4e0b\u53ef\u9006\u5438\u9644\u5230 NucleoMag\u00aeB-beads \u4e0a\u3002\u5728\u5904\u7406\u9ad8\u8fbe 20 mg \u7684\u7ec4\u7ec7\u6837\u672c\u6216 1\u00d710^6 \u4e2a \u7ec6\u80de\u65f6\uff0c\u9996\u5148\u52a0\u5165\u86cb\u767d\u9176 K 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alt=\"\u5e94\u7528\u6570\u636e\" class=\"wp-image-2587\" style=\"width:566px;height:auto\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/001_20240513205647.jpg 923w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/001_20240513205647-300x158.jpg 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/001_20240513205647-768x404.jpg 768w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/001_20240513205647-576x303.jpg 576w\" sizes=\"(max-width: 923px) 100vw, 923px\" \/><\/figure>\n\n\n\n<p><strong>\u4eceHeLa\u7ec6\u80de\u4e2d\u63d0\u53d6DNA<\/strong><\/p>\n\n\n\n<p>\u5229\u7528 Opentrons Flex\u2122 \u5de5\u4f5c\u7ad9\u642d\u914d NucleoMag\u00ae Tissue \u8bd5\u5242\u76d2\uff0c\u6210\u529f\u4ece2.4\u00d710^5 \u4e2a HeLa \u7ec6\u80de\u4e2d\u63d0\u53d6\u4e86\u9ad8\u7eaf\u5ea6 DNA\u3002\u901a\u8fc7\u4f7f TapeStation \u5bf9\u7eaf\u5316\u540e\u7684 DNA \u8fdb\u884c\u8bc4\u4f30\uff0c\u5305\u62ec\u8bc4\u4f30 DNA \u5b8c\u6574\u6027\uff08DIN\uff09\u548c\u7247\u6bb5\u5927\u5c0f\u5206\u5e03\uff08\u865a\u62df\u51dd\u80f6\u6210\u50cf\uff09\uff0c\u7ed3\u679c\u663e\u793a DNA \u7684\u5e73\u5747 DIN \u503c\u4e3a9.0\u00b10.09\u3002\u8fd9\u8868\u660e Opentrons Flex\u2122 \u5de5\u4f5c\u7ad9\u4e0e NucleoMag\u00ae Tissue\u8bd5\u5242\u76d2\u7ed3\u5408\u4f7f\u7528\uff0c\u80fd\u591f\u7a33\u5b9a\u5730\u4ece HeLa \u7ec6\u80de\u4e2d\u63d0\u53d6\u9ad8\u8d28\u91cf\u7684 DNA \u3002<\/p>\n\n\n\n<p><strong>\u503c\u5f97\u4fe1\u8d56\u7684 qPCR \u68c0\u6d4b\u7ed3\u679c<\/strong><\/p>\n\n\n\n<p>\u901a\u8fc7\u8fdb\u884c qPCR \u5206\u6790\uff08\u7528\u84dd\u8272\u6761\u8868\u793a\uff09\uff0c\u4f7f\u7528 PowerTrackTM SYBR GreenMaster Mix \u4f5c\u4e3a\u8bd5\u5242\uff0c\u5f15\u7269\u7ecf IDT \u8bbe\u8ba1\uff0c\u9776\u5411\u57fa\u56e0\u7ec4\u4e2d \u03b2-2- \u5fae\u7403\u86cb\u767d\u7684\u4e00\u4e2a 190 bp \u533a\u57df\u3002qPCR 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