{"id":2811,"date":"2024-05-30T21:38:11","date_gmt":"2024-05-30T13:38:11","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2811"},"modified":"2024-09-03T12:00:12","modified_gmt":"2024-09-03T04:00:12","slug":"fully-automatic-pipetting","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/fully-automatic-pipetting\/","title":{"rendered":"\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u8bf4\u660e\u624b\u518c"},"content":{"rendered":"
\n
\"\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\"<\/figure><\/div>\n\n\n

\u524d\u8a00<\/strong>
\u6b22\u8fce\u9605\u8bfb Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u8bf4\u660e\u624b\u518c\u3002\u672c\u624b\u518c\u4e3a\u60a8\u65e5\u5e38\u8bbe\u7f6e\u548c\u4f7f\u7528 Flex \u5168
\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u63d0\u4f9b\u5168\u9762\u6307\u5bfc\uff0c\u4fa7\u91cd\u4e8e\u5728\u5b9e\u9a8c\u5ba4\u73af\u5883\u4e2dFlex\u65e5\u5e38\u7528\u6237\u6700\u4e3a\u5173\u5207\u7684\u8bf8\u591a\u8bdd\u9898\u3002<\/p>\n\n\n\n

\u624b\u518c\u7ed3\u6784<\/strong>
Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u662f\u4e00\u5957\u590d\u6742\u7cfb\u7edf\uff0c\u60a8\u53ef\u4ee5\u4ece\u4e0d\u540c\u9014\u5f84\u4e86\u89e3\u8fd9\u5957\u7cfb\u7edf\uff0c\u5fc5\u8981
\u65f6\u4e5f\u53ef\u4ee5\u76f4\u63a5\u8df3\u5230\u60a8\u611f\u5174\u8da3\u7684\u7ae0\u8282\u9605\u8bfb\uff01\u6bd4\u5982\uff0c\u5982\u679c\u60a8\u5b9e\u9a8c\u5ba4\u91cc\u5df2\u7ecf\u59a5\u5584\u5b89\u88c5\u4e86 Flex \u5168\u81ea
\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\uff0c\u90a3\u4e48\u60a8\u53ef\u4ee5\u8df3\u8fc7\u5b89\u88c5\u4e0e\u79fb\u52a8\u7ae0\u8282\u3002<\/p>\n\n\n\n

\u5982\u679c\u60a8\u66f4\u559c\u6b22\u5f15\u5bfc\u5f0f\u9605\u8bfb\uff0c\u672c\u624b\u518c\u505a\u4e86\u7cbe\u5fc3\u7684\u7ed3\u6784\u8bbe\u8ba1\uff0c\u60a8\u53ef\u4ee5\u4ece\u5f00\u7bc7\u5230\u7ed3\u5c3e\u8fdb\u884c\u7cfb\u7edf\u9605\u8bfb\u3002
\u25aa \u5173\u4e8e Flex\u3002\u7b2c\u4e00\u7ae0\u7b80\u4ecb Flex \u7684\u4e3b\u8981\u7279\u6027\uff0c\u4ee5\u53ca\u91cd\u8981\u7684\u5b89\u5168\u4fe1\u606f\u548c\u76d1\u7ba1\u4fe1\u606f\u3002
\u25aa \u5f00\u7bb1 Flex\u3002\u5982\u679c\u60a8\u9700\u8981\u5b89\u88c5 Flex\uff0c\u8bf7\u6309\u7167\u7b2c\u4e8c\u7ae0\u5b89\u88c5\u4e0e\u79fb\u52a8\u4e2d\u7684\u8be6\u7ec6\u8bf4\u660e\u5b89\u88c5\uff0c\u7136\u540e\u4ece
\u7b2c\u4e09\u7ae0\u7cfb\u7edf\u63cf\u8ff0\u4e2d\u4e86\u89e3 Flex \u7684\u57fa\u672c\u7ec4\u4ef6\u3002
\u25aa \u8bbe\u7f6e\u7532\u677f\u3002\u914d\u7f6e\u7532\u677f\uff0c\u542f\u7528 Flex \u5e94\u7528\u7a0b\u5e8f\u3002\u7b2c\u56db\u7ae0\u6a21\u5757\u4ecb\u7ecd\u53ef\u4ee5\u5d4c\u5165\u6216\u642d\u8f7d\u5728\u7532\u677f\u4e0a
\u5de5\u4f5c\u7684 Opentrons \u5916\u56f4\u6a21\u5757\u6216\u786c\u4ef6\u3002\u7b2c\u4e94\u7ae0\u5b9e\u9a8c\u5ba4\u8bf4\u660e\u5982\u4f55\u4f7f\u7528\u673a\u5668\u79fb\u6db2\u3002
\u25aa \u8fd0\u884c\u534f\u8bae\u3002Flex \u7684\u4e3b\u8981\u7528\u9014\u5c31\u662f\u8fd0\u884c\u6807\u51c6\u5316\u79d1\u5b66\u7a0b\u5e8f\uff0c\u5373\u534f\u8bae\u3002\u7b2c\u516d\u7ae0\u534f\u8bae\u5f00\u53d1\u4ecb\u7ecd\u4e86
\u5982\u4f55\u83b7\u5f97\u73b0\u6210\u7684\u534f\u8bae\u6216\u81ea\u884c\u8bbe\u8ba1\u534f\u8bae\u3002\u7b2c\u4e03\u7ae0\u8f6f\u4ef6\u4e0e\u64cd\u4f5c\u4ecb\u7ecd\u5982\u4f55\u8fd0\u884c\u534f\u8bae\uff0c\u5982\u4f55\u6267\u884c
\u5176\u5b83\u4efb\u52a1\u548c\u81ea\u5b9a\u4e49\u673a\u5668\u8bbe\u7f6e\u3002
\u25aa Flex \u7ef4\u62a4\u3002\u6309\u7167\u7b2c\u516b\u7ae0\u7ef4\u62a4\u4e0e\u670d\u52a1\u5efa\u8bae\uff0c\u53ca\u65f6\u6e05\u6d01 Flex\uff0c\u4fdd\u6301\u6700\u4f73\u8fd0\u884c\u72b6\u6001\uff1b\u6216\u7533\u8bf7
Opentrons \u670d\u52a1\u9879\u76ee\uff0c\u8ba9\u6211\u4eec\u5e2e\u60a8\u7ef4\u62a4\u3002
\u25aa \u66f4\u591a\u5176\u4ed6\u4fe1\u606f\u3002\u8fd8\u60f3\u4e86\u89e3\u5176\u5b83\uff1f\u53ef\u4ee5\u67e5\u770b\u9644\u5f55\u3002
\u25aa \u9644\u5f55 A \u672f\u8bed\u8868\u5b9a\u4e49\u4e86 Flex \u76f8\u5173\u672f\u8bed\u3002
\u25aa \u9644\u5f55 B \u5176\u5b83\u8d44\u6599\u4ecb\u7ecd\u66f4\u591a\u6709\u5173 Opentrons \u4ea7\u54c1\u548c\u65b9\u4fbf\u7f16\u5199 Flex \u63a7\u5236\u4ee3\u7801\u7684\u8d44\u6e90\u3002
\u25aa \u9644\u5f55 C \u5f00\u6e90\u8f6f\u4ef6\u4ecb\u7ecd\u4e86\u4f9b\u5f00\u53d1\u8005\u548c\u975e\u5f00\u53d1\u8005\u4f7f\u7528\u7684 GitHub Opentrons \u8f6f\u4ef6\u8d44\u6e90\u3002
\u25aa \u9644\u5f55 D \u652f\u6301\u4e0e\u8054\u7cfb\u65b9\u5f0f\u5217\u660e\u4e86 Opentrons \u8054\u7cfb\u65b9\u5f0f\uff0c\u65b9\u4fbf\u60a8\u5411\u6211\u4eec\u5bfb\u6c42\u5176\u5b83\u5e2e\u52a9\u3002<\/p>\n\n\n\n

\u6ce8\u610f\u4e0e\u8b66\u544a<\/strong>
\u672c\u624b\u518c\u4e2d\u6709\u4e00\u4e9b\u4e13\u95e8\u7684\u6ce8\u610f\u548c\u8b66\u544a\u6807\u793a\u3002\u201c\u6ce8\u610f\u201d\uff0c\u4e3a\u60a8\u63d0\u4f9b\u4e00\u4e9b\u65e5\u5e38\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u4e0d\u4f1a\u6ce8
\u610f\u5230\u7684\u5b9e\u7528\u4fe1\u606f\u3002\u201c\u8b66\u544a\u201d\uff0c\u63d0\u9192\u60a8\u8981\u7279\u522b\u91cd\u89c6\uff0c\u5b83\u7684\u51fa\u73b0\u610f\u5473\u7740\u53ef\u80fd\u4f1a\u5f15\u53d1\u4eba\u8eab\u4f24\u5bb3\u3001\u8bbe\u5907
\u635f\u574f\u3001\u6837\u54c1\u6216\u8bd5\u5242\u635f\u8017\u6216\u6c61\u67d3\u3001\u6570\u636e\u4e22\u5931\u6216\u5176\u4ed6\u4f24\u5bb3\u98ce\u9669\u3002<\/p>\n\n\n\n

\u7b2c\u4e00\u7ae0\u7b80\u4ecb<\/strong>
\u672c\u7ae0\u4ecb\u7ecd Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u751f\u6001\u7cfb\u7edf\uff0c\u5305\u62ec\u6574\u4f53\u7cfb\u7edf\u8bbe\u8ba1\u548c\u53ef\u7528\u7684\u5de5\u4f5c\u7ad9
\u914d\u7f6e\uff0c\u4ee5\u53ca\u60a8\u5b89\u88c5 Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u65f6\uff0c\u8981\u4e86\u89e3\u7684\u91cd\u8981\u5408\u89c4\u4e0e\u5b89\u5168\u4fe1\u606f\u3002
\u5173\u4e8e Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u7684\u66f4\u591a\u8be6\u7ec6\u4fe1\u606f\uff0c\u8bf7\u53c2\u8003\u7b2c\u4e09\u7ae0\u7cfb\u7edf\u63cf\u8ff0\u3002<\/p>\n\n\n\n


1.1 \u6b22\u8fce\u4f7f\u7528 Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9<\/strong>
Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\uff0c\u662f\u4e00\u6b3e\u9002\u7528\u4e8e\u9ad8\u901a\u91cf\u590d\u6742\u5de5\u4f5c\u6d41\u7684\u79fb\u6db2\u673a\u5668\u3002Flex \u79fb\u6db2
\u5de5\u4f5c\u7ad9\u662f\u6a21\u5757\u5316\u7cfb\u7edf\u7684\u57fa\u7840\u5e73\u53f0\uff0c\u60a8\u53ef\u4ee5\u6839\u636e\u9700\u8981\u5728\u4e0a\u9762\u81ea\u884c\u642d\u8f7d\u79fb\u6db2\u5668\u3001\u8f6c\u677f\u6293\u624b\u3001\u7532
\u677f\u4e0a\u6a21\u5757\u548c\u5b9e\u9a8c\u5668\u76bf\u7b49\u6a21\u5757\u7cfb\u7edf\u3002\u60a8\u53ef\u4ee5\u901a\u8fc7 Flex \u89e6\u6478\u5c4f\u76f4\u63a5\u64cd\u4f5c\u673a\u5668\uff0c\u4e5f\u53ef\u4ee5\u901a\u8fc7
Opentrons App \u6216\u5f00\u6e90 API \u5728\u5b9e\u9a8c\u5ba4\u5176\u5b83\u5730\u65b9\u8fdc\u7a0b\u64cd\u4f5c\u673a\u5668\u3002
Opentrons Flex \u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\uff0c\u63d0\u4f9b\u60a8\u542f\u52a8\u81ea\u52a8\u5316\u5e38\u89c4\u5b9e\u9a8c\u5ba4\u5de5\u4f5c\u9700\u8981\u7684\u6240\u6709\u8bbe\u5907\uff0c\u5305
\u62ec\u673a\u5668\u3001\u786c\u4ef6\u548c\u5b9e\u9a8c\u5ba4\u5668\u76bf\u3002\u5173\u4e8e\u5176\u4ed6\u5e94\u7528\uff0cOpentrons Flex \u5b8c\u5168\u57fa\u4e8e\u5f00\u6e90\u8f6f\u4ef6\u548c\u56fa\u4ef6\u8fd0
\u884c\uff0c\u65e0\u8bd5\u5242\u548c\u5b9e\u9a8c\u5ba4\u5668\u76bf\u9650\u5236\uff0c\u60a8\u53ef\u4ee5\u81ea\u4e3b\u8bbe\u8ba1\u548c\u8fd0\u884c\u534f\u8bae\u3002<\/p>\n\n\n\n


Flex \u65b0\u529f\u80fd<\/strong>
Opentrons Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u662f Opentrons \u7cfb\u5217\u79fb\u6db2\u673a\u5668\u4e4b\u4e00\u3002Opentrons Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u7528\u6237
\u53ef\u80fd\u4e5f\u77e5\u9053 Opentrons OT-2 \u79fb\u6db2\u5de5\u4f5c\u7ad9\u3002\u5728\u8bb8\u591a\u5173\u952e\u65b9\u9762\uff0cFlex \u90fd\u6bd4 OT-2 \u66f4\u80dc\u4e00\u7b79\uff0c\u901a\u91cf
\u66f4\u5927\uff0c\u81ea\u52a8\u5316\u7a0b\u5ea6\u66f4\u9ad8\u3002<\/p>\n\n\n\n

\"\u7279\u6027\u63cf\u8ff0\"<\/figure>\n\n\n\n
\"\u7279\u6027\u63cf\u8ff0\"<\/figure>\n\n\n\n

Opentrons \u7f51\u7ad9\u63d0\u4f9b\u8be6\u7ec6\u7684\u673a\u5668\u6280\u672f\u53c2\u6570\u5bf9\u6bd4\u3002<\/strong>
Flex \u548c OT-2 \u673a\u5668\u90fd\u57fa\u4e8e\u5f00\u6e90\u8f6f\u4ef6\u8fd0\u884c\uff0cOpentrons App \u80fd\u540c\u65f6\u63a7\u5236\u8fd9\u4e24\u79cd\u673a\u5668\u3002\u867d\u7136 OT-2
\u534f\u8bae\u65e0\u6cd5\u76f4\u63a5\u5728 Flex \u4e0a\u8fd0\u884c\uff0c\u4f46\u6539\u7f16\u5f88\u7b80\u5355\uff08\u53c2\u8003\u534f\u8bae\u5f00\u53d1 6.4 OT-2 \u534f\u8bae\u90e8\u5206\uff09\u3002<\/p>\n\n\n\n

Flex \u5de5\u4f5c\u7ad9<\/strong>
Opentrons Flex \u5de5\u4f5c\u7ad9\u7531\u673a\u5668\u3001\u9644\u4ef6\u3001\u79fb\u6db2\u5668\u548c\u8f6c\u677f\u6293\u624b\u3001\u6a21\u5757\u3001\u4ee5\u53ca\u5b9e\u73b0\u7279\u5b9a\u81ea\u52a8\u5316\u5e94\u7528
\u6240\u9700\u7684\u5668\u76bf\u7ec4\u6210\u3002\u5de5\u4f5c\u7ad9\u6240\u6709\u7ec4\u4ef6\u90fd\u662f\u6a21\u5757\u5f0f\u7ec4\u4ef6\u3002\u5982\u679c\u60a8\u60f3\u8981\u53d8\u66f4\u7528\u9014\uff0c\u53ef\u4ee5\u65b0\u589e\u6216\u66f4\u6362
\u5176\u5b83 Flex \u786c\u4ef6\u548c\u517c\u5bb9\u8017\u6750\u3002
NGS \u5de5\u4f5c\u7ad9<\/strong>
Opentrons Flex NGS \u5de5\u4f5c\u7ad9\u652f\u6301\u81ea\u52a8\u5236\u5907 NGS \u6587\u5e93\uff0c\u4f7f\u7528\u4e3b\u6d41\u8bd5\u5242\u7cfb\u7edf\uff0c\u81ea\u52a8\u6267\u884c\u6d4b\u5e8f\u524d
\u5de5\u4f5c\u6d41\u7a0b\uff0c\u4f8b\u5982\u7247\u6bb5\u5316\u3001\u9776\u5411\u6587\u5e93\u5236\u5907\u3002<\/p>\n\n\n\n

\u9664\u4e86 Flex \u673a\u5668\uff0cNGS \u5de5\u4f5c\u7ad9\u8fd8\u5305\u62ec\uff1a
\u25fe \u8f6c\u677f\u6293\u624b
\u25fe \u4e24\u4e2a 8 \u901a\u9053\u79fb\u6db2\u5668\uff081-50\u03bcL \u548c 5-1000\u03bcL\uff09
\u25fe \u73af\u5f62\u78c1\u529b\u67b6
\u25fe \u6e29\u63a7\u6a21\u5757
\u25fe \u70ed\u5faa\u73af\u6a21\u5757
\u25fe \u914d\u5957\u5b9e\u9a8c\u5ba4\u5668\u76bf\uff08\u6ee4\u82af\u5438\u5934\u3001\u5fae\u91cf\u79bb\u5fc3\u7ba1\u3001\u50a8\u6db2\u69fd\u548c PCR \u5b54\u677f\uff09
PCR \u5de5\u4f5c\u7ad9
Opentrons Flex PCR \u5de5\u4f5c\u7ad9\uff0c\u80fd\u540c\u65f6\u4e3a\u6700\u591a 96 \u4efd\u6837\u672c\u81ea\u52a8\u8fdb\u884c PCR \u4f53\u7cfb\u914d\u7f6e\u548c\u542f\u52a8\u70ed\u5faa\u73af\u6d41\u7a0b\u3002\u673a\u5668\u53ef\u4ee5\u5c06\u51b7\u5374\u540e\u7684\u8bd5\u5242\u548c\u6837\u672c\u5e73\u5747\u5206\u914d\u5230 96 PCR \u5b54\u677f\u7684\u6bcf\u4e2a\u5b54\u4e2d\u3002\u7ed3\u5408\u81ea\u52a8\u70ed\u5faa\u73af\u6a21\u5757\uff0c\u4f7f\u7528\u8f6c\u677f\u6293\u624b\u5c06\u5b54\u677f\u653e\u5165\u70ed\u5faa\u73af\u4eea\u5185\uff0c\u7136\u540e\u8fd0\u884c\u9009\u5b9a\u7684 PCR \u7a0b\u5e8f\u5373\u53ef\u3002<\/p>\n\n\n\n

\u9664 Flex \u673a\u5668\u4ee5\u5916\uff0cPCR \u5de5\u4f5c\u7ad9\u8fd8\u5305\u62ec\uff1a
\u25fe \u8f6c\u677f\u6293\u624b
\u25fe \u5355\u901a\u9053\u79fb\u6db2\u5668\uff081-50\u03bcL\uff09
\u25fe 8 \u901a\u9053\u79fb\u6db2\u5668\uff081-50\u03bcL\uff09
\u25fe \u6e29\u63a7\u6a21\u5757
\u25fe \u914d\u5957\u5b9e\u9a8c\u5ba4\u5668\u76bf\uff08\u6ee4\u82af\u5438\u5934\u3001\u5fae\u91cf\u79bb\u5fc3\u7ba1\u3001\u50a8\u6db2\u69fd\u548c PCR \u5b54\u677f\uff09<\/p>\n\n\n\n

\u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9
Opentrons Flex \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\u80fd\u81ea\u52a8\u8fdb\u884c DNA\/RNA \u5206\u79bb\u7eaf\u5316\uff0c\u7528\u73af\u5f62\u78c1\u529b\u67b6\u5206\u79bb\u78c1\u73e0\uff0c
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\u25fe \u73af\u5f62\u78c1\u529b\u67b6
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\u78c1\u73e0\u86cb\u767d\u7eaf\u5316\u5de5\u4f5c\u7ad9
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