{"id":2821,"date":"2024-05-30T22:20:39","date_gmt":"2024-05-30T14:20:39","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2821"},"modified":"2024-09-03T14:15:08","modified_gmt":"2024-09-03T06:15:08","slug":"whxyzdhhszb","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/whxyzdhhszb\/","title":{"rendered":"\u4e3a\u4f55\u9700\u8981\u81ea\u52a8\u5316\u6838\u9178\u5236\u5907"},"content":{"rendered":"\n

\u6838\u9178\u5206\u79bb\u662f\u5206\u5b50\u751f\u7269\u5b66\u7684\u4e00\u9879\u57fa\u77f3\u6280\u672f\u3002\u8bb8\u591a\u5b9e\u9a8c\u5ba4\u4e3a\u6b64\u4f7f\u7528\u624b\u52a8\u7eaf\u5316\u65b9\u6848\uff0c\u4f46\u5b83\u4eec\u6709\u56db\u4e2a\u7f3a\u70b9\u3002
\u6838\u9178\u5206\u79bb\u662f\u5206\u5b50\u751f\u7269\u5b66\u7684\u4e00\u9879\u57fa\u77f3\u6280\u672f\uff0c\u4e3a\u5927\u591a\u6570\u6e7f\u5b9e\u9a8c\u5ba4\u5e94\u7528\u63d0\u4f9b\u8d77\u59cb\u6750\u6599\u3002\u7136\u800c\uff0c\u5206\u79bb\u6750\u6599\u53ea\u662f\u6210\u529f\u7684\u4e00\u534a\u3002\u5206\u79bb\u6838\u9178\u7684\u7eaf\u5316\u5bf9\u4e8e\u53ef\u9760\u548c\u4e00\u81f4\u7684\u6570\u636e\u4ee5\u53ca\u6574\u4f53\u5b9e\u9a8c\u6210\u529f\u540c\u6837\u91cd\u8981\u3002\u4f8b\u5982\uff1a<\/p>\n\n\n\n

\u53bb\u9664\u53ef\u80fd\u5e72\u6270\u4e0b\u6e38\u53cd\u5e94\u7684\u6291\u5236\u5242\u548c\u6742\u8d28\uff0c\u4f8b\u5982 PCR \u548c\u9006\u8f6c\u5f55\u9176\u6291\u5236\u5242\u3002\u6291\u5236\u5242\u548c\u6742\u8d28\u53ef\u80fd\u6765\u81ea\u6837\u54c1\u672c\u8eab\uff08\u4f8b\u5982\uff0c\u5728\u590d\u6742\u7684\u73af\u5883\u6837\u54c1\u7684\u60c5\u51b5\u4e0b\uff09\u3001\u5206\u79bb\u8fc7\u7a0b\u4e2d\u4f7f\u7528\u7684\u5316\u5b66\u54c1\uff08\u4f8b\u5982\uff0c\u6d17\u6da4\u5242\u3001\u6709\u673a\u6eb6\u5242\uff09\u548c\/\u6216\u5de5\u4f5c\u533a\u57df\u4e2d\u7684\u6c61\u67d3\u3002
\u7eaf\u51c0\u7684\u8d77\u59cb\u6750\u6599\u5bf9\u4e8e NGS \u6837\u54c1\u5236\u5907\u81f3\u5173\u91cd\u8981\u3002\u5728\u9776\u6807\u5bcc\u96c6\u3001\u6587\u5e93\u6784\u5efa\u548c\u5c3a\u5bf8\u9009\u62e9\u8fc7\u7a0b\u4e2d\u5b58\u5728\u7684\u4efb\u4f55\u6742\u8d28\u6216\u4e0d\u9700\u8981\u7684\u6750\u6599\uff08\u4f8b\u5982\uff0c\u5f15\u7269\u4e8c\u805a\u4f53\uff09\u90fd\u53ef\u80fd\u5bfc\u81f4\u4f2a\u5f71\uff0c\u4f7f\u6d4b\u5e8f\u6570\u636e\u53d8\u5f97\u6beb\u65e0\u610f\u4e49\uff0c\u53ef\u80fd\u5bfc\u81f4\u5927\u91cf\u65f6\u95f4\u548c\u91d1\u94b1\u7684\u635f\u5931\u3002<\/p>\n\n\n\n

\"\u81ea\u52a8\u5316\u6838\u9178\u5236\u5907\"<\/figure>\n\n\n\n


\u4ece\u53cd\u5e94\u6df7\u5408\u7269\u548c\u743c\u8102\u7cd6\u51dd\u80f6\u4e2d\u7eaf\u5316\u6240\u9700\u7684 PCR \u6269\u589e\u5b50\u3001\u8d28\u7c92\u3001\u9650\u5236\u6027\u6d88\u5316\u7684 DNA \u7247\u6bb5\u548c RNA\uff08\u4f8b\u5982\u5c0f RNA \u7269\u79cd\uff09\u662f\u5404\u79cd\u5e94\u7528\u7684\u5148\u51b3\u6761\u4ef6\u6280\u672f\uff0c\u5305\u62ec\u514b\u9686\u3001\u57fa\u56e0\u6539\u9020\u3001RNA \u548c DNA \u8868\u5f81\u3001\u4f53\u5916\u8f6c\u5f55\u548c\u7ffb\u8bd1\u7b49\u3002
\u624b\u52a8\u7eaf\u5316\u65b9\u6848\u6709\u5176\u7f3a\u70b9
\u5982\u4eca\uff0c\u5206\u5b50\u751f\u7269\u5b66\u5bb6\u6709\u591a\u79cd\u6613\u4e8e\u4f7f\u7528\u7684\u8bd5\u5242\u76d2\u53ef\u4f9b\u9009\u62e9\uff0c\u8fd9\u4e9b\u8bd5\u5242\u76d2\u53ef\u4ee5\u4ece\u51e0\u4e4e\u4efb\u4f55\u53ef\u4ee5\u60f3\u8c61\u5230\u7684\u6837\u54c1\u7c7b\u578b\u4e2d\u8fdb\u884c\u65e0\u7f1d\u6838\u9178\u5206\u79bb\u548c\u7eaf\u5316\u3002\u7136\u800c\uff0c\u867d\u7136\u8fd9\u4e9b\u8bd5\u5242\u76d2\u4e2d\u7684\u8bb8\u591a\u5728\u7eaf\u5316\u6838\u9178\u65b9\u9762\u975e\u5e38\u6709\u6548\uff0c\u4f46\u5b83\u4eec\u901a\u5e38\u662f\u4e3a\u624b\u52a8\u64cd\u4f5c\u800c\u8bbe\u8ba1\u7684\uff0c\u8fd9\u5e26\u6765\u4e86\u51e0\u4e2a\u95ee\u9898\uff1a<\/p>\n\n\n\n

\u6c61\u67d3\u98ce\u9669\u3002\u4e0e\u6240\u6709\u6d89\u53ca\u79fb\u6db2\u7684\u624b\u52a8\u5de5\u4f5c\u6d41\u7a0b\u4e00\u6837\uff0c\u624b\u52a8\u7eaf\u5316\u5b58\u5728\u6837\u54c1\u95f4\u4ea4\u53c9\u6c61\u67d3\u7684\u98ce\u9669\u3002\u4f7f\u7528\u591a\u901a\u9053\u79fb\u6db2\u53ef\u4ee5\u964d\u4f4e\u8fd9\u79cd\u98ce\u9669\uff0c\u4f46\u6b64\u9009\u9879\u4ec5\u9002\u7528\u4e8e\u57fa\u4e8e\u677f\u7684\u65b9\u6848\u3002\u5373\u4f7f\u4f7f\u7528\u591a\u901a\u9053\u79fb\u6db2\uff0c\u6709\u65f6\u4e5f\u96be\u514d\u4f1a\u51fa\u9519\uff0c\u5c24\u5176\u662f\u5728\u5904\u7406\u591a\u4e2a\u8f6e\u6b21\u7684\u76f8\u5bf9\u5927\u91cf\u6837\u672c\u65f6\u53ef\u80fd\u4ea7\u751f\u7684\u75b2\u52b3\u3002
\u96be\u4ee5\u6269\u5927\u89c4\u6a21\u3002\u5bf9\u4e8e\u8bd5\u7ba1\u683c\u5f0f\u7684\u65b9\u6848\uff0c\u5de5\u4f5c\u4eba\u5458\u901a\u5e38\u53d7\u5230\u4e00\u6b21\u5728\u79bb\u5fc3\u673a\u548c\u5176\u4ed6\u5fc5\u8981\u8bbe\u5907\u4e2d\u53ef\u4ee5\u5904\u7406\u7684\u8bd5\u7ba1\u6570\u91cf\u7684\u9650\u5236\u3002\u4e00\u79cd\u5e38\u89c1\u7684\u89e3\u51b3\u65b9\u6cd5\u662f\u5206\u6279\u5904\u7406\u5927\u91cf\u6837\u672c\uff0c\u4f46\u8fd9\u79cd\u65b9\u6cd5\u901f\u5ea6\u6162\uff0c\u9700\u8981\u5927\u91cf\u7684\u52a8\u624b\u5de5\u4f5c\u3002\u56e0\u6b64\uff0c\u624b\u52a8\u51c0\u5316\u5bf9\u4e8e\u5927\u89c4\u6a21\u64cd\u4f5c\u6765\u8bf4\u4e0d\u662f\u4e00\u79cd\u53ef\u884c\u7684\u9009\u62e9\u3002
\u5bb9\u6613\u51fa\u9519\u3002\u9664\u4e86\u6837\u672c\u6b8b\u7559\u7684\u98ce\u9669\u5916\uff0c\u624b\u52a8\u65b9\u6848\u8fd8\u5b58\u5728\u5176\u4ed6\u9519\u8bef\u98ce\u9669\u3002\u60f3\u8c61\u4e00\u4e0b\u8fd9\u6837\u4e00\u4e2a\u573a\u666f\uff1a\u60a8\u5df2\u7ecf\u6807\u8bb0\u4e86 24 \u4e2a\u6837\u672c\u7ba1\uff0c\u4f46\u5728\u5bf9\u5de5\u4f5c\u53f0\u8fdb\u884c\u6d88\u6bd2\u65f6\uff0c\u65b9\u6848\u8fdb\u884c\u5230\u4e00\u534a\u65f6\u6807\u7b7e\u88ab\u6d17\u6389\u4e86\u3002\u5728\u8bd5\u56fe\u5c06\u6c61\u67d3\u98ce\u9669\u4fdd\u6301\u5728\u4f4e\u6c34\u5e73\u65f6\uff0c\u8fd9\u662f\u4e00\u4e2a\u5f88\u5bb9\u6613\u9677\u5165\u7684\u9677\u9631\uff0c\u4f46\u5f88\u96be\u4ece\u4e2d\u6062\u590d\u8fc7\u6765\u3002\u8fd9\u79cd\u60c5\u51b5\u53ef\u80fd\u4f1a\u6bc1\u6389\u6574\u4e2a\u5b9e\u9a8c\u3002
\u8017\u65f6\u3002\u77ed\u65f6\u95f4\u7684\u5b75\u80b2\u6b65\u9aa4\u548c\u77ed\u6682\u7684\u79bb\u5fc3\u65cb\u8f6c\u4f7f\u5f97\u6bcf\u6b21\u8fd0\u884c\u7684\u53ef\u64cd\u4f5c\u65f6\u95f4\u975e\u5e38\u77ed\uff0c\u800c\u6279\u91cf\u5904\u7406\u6837\u54c1\u7684\u9700\u6c42\u5c06\u5360\u7528\u60a8\u66f4\u591a\u7684\u5b9d\u8d35\u65f6\u95f4\u3002\u5982\u679c\u7eaf\u5316\u662f\u5b9e\u9a8c\u5ba4\u5de5\u4f5c\u6d41\u7a0b\u7684\u5e38\u89c4\u90e8\u5206\uff0c\u5b83\u53ef\u4ee5\u5e2e\u52a9\u60a8\u7684\u5b9e\u9a8c\u5ba4\u6210\u5458\u66f4\u597d\u5730\u5229\u7528\u4ed6\u4eec\u7684\u65f6\u95f4\u3002
\u81ea\u52a8\u5316\u6d88\u9664\u624b\u52a8\u8fc7\u7a0b\u9519\u8bef\u5e76\u63d0\u9ad8\u901f\u5ea6\u548c\u751f\u4ea7\u529b
\u4e0a\u8ff0\u95ee\u9898\u53ef\u80fd\u4f1a\u5355\u72ec\u6216\u7ec4\u5408\u5f71\u54cd\u6570\u636e\u8d28\u91cf\u548c\u5b9e\u9a8c\u8f93\u51fa\u3002\u5982\u679c\u60a8\u6b63\u5728\u7814\u7a76\u6838\u9178\uff0c\u60a8\u5df2\u7ecf\u77e5\u9053\u7eaf\u5316\u7684\u91cd\u8981\u6027\u3002\u9009\u62e9\u81ea\u52a8\u5316\u8bbe\u7f6e\u5c06\u5141\u8bb8\u60a8\u89e3\u51b3\u4e0e\u624b\u52a8\u534f\u8bae\u76f8\u5173\u7684\u95ee\u9898\uff0c\u5e2e\u52a9\u60a8\u83b7\u5f97\u4e00\u81f4\u7684\u6570\u636e\uff0c\u6700\u5927\u9650\u5ea6\u5730\u51cf\u5c11\u9519\u8bef\uff0c\u63d0\u9ad8\u901f\u5ea6\u548c\u541e\u5410\u91cf\uff0c\u5e76\u8ba9\u60a8\u6709\u66f4\u591a\u7684\u53ef\u64cd\u4f5c\u65f6\u95f4\u82b1\u5728\u5176\u4ed6\u66f4\u4ee4\u4eba\u5174\u594b\u7684\u5b9e\u9a8c\u5ba4\u4efb\u52a1\u4e0a\uff01\u4ece\u957f\u8fdc\u6765\u770b\uff0c\u60a8\u7684\u5b9e\u9a8c\u5ba4\u751a\u81f3\u53ef\u4ee5\u901a\u8fc7\u6295\u8d44\u81ea\u52a8\u5316\u6765\u8282\u7701\u8d44\u91d1\u3002<\/p>\n\n\n\n

\u90a3\u4f60\u8fd8\u5728\u7b49\u4ec0\u4e48\uff1f\u6709\u4e86\u79fb\u6db2\u673a\u5668\u4eba\u548c\u6b63\u786e\u7684\u65b9\u6848\uff0c\u60a8\u53ef\u4ee5\u671f\u5f85\u5b9e\u73b0\u4ee5\u4e0b\u7a0b\u5e8f\u7684\u81ea\u52a8\u5316\uff1a<\/p>\n\n\n\n

PCR \u4ea7\u7269\u7eaf\u5316
\u8d28\u7c92\u7eaf\u5316
\u9176\u4fc3\u548c\u6807\u8bb0\u53cd\u5e94\u540e\u7684 DNA \u548c RNA \u6e05\u7406
NGS \u6587\u5e93\u6e05\u7406
PCR \u6291\u5236\u5242\u53bb\u9664
\u8fd8\u6709\u66f4\u591a\uff01
\u5165\u95e8
\u6b63\u5982\u6211\u4eec\u4e0a\u9762\u63d0\u5230\u7684\uff0c\u5f53\u60a8\u62e5\u6709\u4e00\u4e2a\u89e6\u624b\u53ef\u53ca\u7684\u673a\u5668\u4eba\u65f6\uff0c\u4e00\u5207\u7686\u6709\u53ef\u80fd\u3002\u5927\u591a\u6570\u7eaf\u5316\u7a0b\u5e8f\u90fd\u53ef\u4ee5\u9002\u5e94\u81ea\u52a8\u5316\u3002\u60a8\u9700\u8981\u505a\u7684\u5c31\u662f\u5f04\u6e05\u695a\u65b9\u6848\uff0c\u4f46\u60a8\u4e0d\u9700\u8981\u72ec\u81ea\u5b8c\u6210\u8fd9\u9879\u5de5\u4f5c\u3002\u5982\u679c\u60a8\u6b63\u5728\u8003\u8651\u4e3a\u60a8\u7684\u5b9e\u9a8c\u5ba4\u5b9e\u73b0\u81ea\u52a8\u5316\uff0c\u8bf7\u4e0e\u6211\u4eec\u8054\u7cfb\u3002\u6211\u4eec\u5f88\u4e50\u610f\u5e2e\u52a9\u60a8\u5b8c\u6210\u5b83\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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