{"id":2826,"date":"2024-05-30T22:40:43","date_gmt":"2024-05-30T14:40:43","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2826"},"modified":"2024-09-03T14:12:52","modified_gmt":"2024-09-03T06:12:52","slug":"opentrons-covid-19","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/opentrons-covid-19\/","title":{"rendered":"\u4f7f\u7528 Opentrons \u8fdb\u884c COVID-19 \u68c0\u6d4b"},"content":{"rendered":"\n
Opentrons \u7684 COVID-19 \u68c0\u6d4b\u7cfb\u7edf\u53ef\u4ee5\u5e2e\u52a9\u8fd0\u884c\u516c\u5171\u536b\u751f\u76d1\u6d4b\u9879\u76ee\u7684\u5b9e\u9a8c\u5ba4\u7acb\u5373\u6269\u5927\u89c4\u6a21\uff0c\u6bcf\u5929\u7b5b\u67e5 2,400 \u6b21\u68c0\u6d4b\u3002Opentrons \u7684\u4f7f\u547d\u662f\u901a\u8fc7\u66f4\u5e7f\u6cdb\u5730\u4f7f\u7528\u5b9e\u9a8c\u5ba4\u673a\u5668\u4eba\u6765\u52a0\u901f\u751f\u547d\u79d1\u5b66\u7684\u53d1\u5c55\u3002\u4e16\u754c\u73b0\u5728\u6bd4\u4ee5\u5f80\u4efb\u4f55\u65f6\u5019\u90fd\u66f4\u9700\u8981\u8fd9\u79cd\u80fd\u529b\u3002\u5927\u89c4\u6a21\u7684 COVID-19 \u68c0\u6d4b\u662f\u9650\u5236\u6b7b\u4ea1\u7387\u7684\u6700\u4f73\u65b9\u6cd5\u4e4b\u4e00\u3002\u5927\u89c4\u6a21\u68c0\u6d4b\u53ef\u4ee5\u5feb\u901f\u53d1\u73b0\u548c\u9694\u79bb\u611f\u67d3\uff0c\u9650\u5236\u75c5\u6bd2\u7684\u4f20\u64ad\u5e76\u4fdd\u62a4\u8106\u5f31\u4eba\u7fa4\u3002\u4e3a\u4e86\u6b63\u786e\u8c03\u67e5\u4eba\u7fa4\uff0c\u9700\u8981\u5904\u7406\u6570\u767e\u4e07\u4e2a COVID-19 \u68c0\u6d4b\u8bd5\u5242\u76d2\u3002\u4e16\u754c\u5404\u5730\u7684\u7ec4\u7ec7\u90fd\u5728\u52aa\u529b\u5c3d\u5feb\u63d0\u9ad8\u5176\u80fd\u529b\u3002<\/p>\n\n\n\n
\u8fd9\u5c31\u662f Opentrons \u4e3a\u5e94\u5bf9\u8fd9\u79cd\u6311\u6218\u800c\u5efa\u7acb\u7684\u3002<\/p>\n\n\n
Opentrons \u7684 COVID-19 \u68c0\u6d4b\u7cfb\u7edf\u662f\u8fd0\u884c COVID-19 \u516c\u5171\u536b\u751f\u76d1\u6d4b\u9879\u76ee\u7684\u5b9e\u9a8c\u5ba4\u7684\u6700\u4f73\u89e3\u51b3\u65b9\u6848\uff0c\u8fd9\u4e9b\u5b9e\u9a8c\u5ba4\u9700\u8981\u7acb\u5373\u6269\u5927\u5230\u81ea\u52a8\u5316\u64cd\u4f5c\u3002Opentrons \u53ef\u4ee5\u5728\u4e0b\u8ba2\u5355\u540e\u7684\u51e0\u5929\u5185\u5b89\u88c5\u6bcf\u5929\u81ea\u52a8\u8fdb\u884c\u591a\u8fbe 2,400 \u6b21\u6d4b\u8bd5\u7684\u7cfb\u7edf\u3002\u6211\u4eec\u76ee\u524d\u6b63\u5728\u52a0\u5229\u798f\u5c3c\u4e9a\u5dde\u5e15\u6d1b\u963f\u5c14\u6258\u7684\u5f00\u653e\u533b\u5b66\u7814\u7a76\u6240\u90e8\u7f72\u8fd9\u79cd\u76d1\u6d4b\u80fd\u529b\uff0c\u5f88\u5feb\u8fd8\u4f1a\u6709\u66f4\u591a\u76d1\u6d4b\u9879\u76ee\u3002<\/p>\n\n\n\n
\u6211\u4eec\u53ef\u4ee5\u4e0e\u4efb\u4f55\u8bd5\u5242\u4f9b\u5e94\u5546\u5408\u4f5c\uff0c\u751a\u81f3\u63d0\u4f9b\u6700\u4f73\u548c\u6700\u5b9e\u60e0\u7684\u9009\u62e9\u5efa\u8bae\u3002\u5982\u679c\u60a8\u7684\u5b9e\u9a8c\u5ba4\u9700\u8981\u6269\u5927 COVID-19 \u76d1\u6d4b\u4e1a\u52a1\uff0c\u8bf7\u901a\u8fc7 covid-19@opentrons.com \u4e0e\u6211\u4eec\u8054\u7cfb\u3002<\/p>\n\n\n\n
Opentrons \u548c\u5f00\u653e\u533b\u5b66\u7814\u7a76\u6240\u6e34\u671b\u4e0e\u5f53\u5730\u548c\u8054\u90a6\u673a\u6784\u5408\u4f5c\uff0c\u786e\u5b9a\u8be5\u7cfb\u7edf\u662f\u5426\u53ef\u5728\u7d27\u6025\u4f7f\u7528\u6388\u6743 (EUA) \u4e0b\u7528\u4e8e\u4e34\u5e8a\u8bca\u65ad\uff0c\u5e76\u63d0\u4f9b\u6d4b\u8bd5\u5e26\u5bbd\u4ee5\u6ee1\u8db3\u65e5\u76ca\u589e\u957f\u7684\u6781\u9ad8\u9700\u6c42\u3002<\/p>\n\n\n\n
\u4ee5\u4e0b\u662f\u6709\u5173\u6211\u4eec\u5f00\u53d1\u7684\u7cfb\u7edf\u4ee5\u53ca\u5982\u4f55\u5c06\u5176\u90e8\u7f72\u7528\u4e8e\u5927\u89c4\u6a21 COVID-19 \u6d4b\u8bd5\u7684\u8be6\u7ec6\u4fe1\u606f\u3002<\/p>\n\n\n\n
\u6d4b\u8bd5\u8fc7\u7a0b\u4e0e\u8bb8\u591a\u4f20\u67d3\u75c5\u68c0\u6d4b\u4e00\u6837\uff0c\u68c0\u6d4b COVID-19 \u75c5\u6bd2\u7684\u68c0\u6d4b\u91c7\u7528\u5b9a\u91cf\u805a\u5408\u9176\u94fe\u53cd\u5e94 (qPCR) \u6280\u672f\u3002\u5bf9\u4e8e\u50cf COVID-19 \u8fd9\u6837\u7684\u51a0\u72b6\u75c5\u6bd2\uff0c\u8be5\u68c0\u6d4b\u5b9e\u9645\u4e0a\u662f\u4e00\u79cd\u9006\u8f6c\u5f55\u9176 qPCR (RT-qPCR)\uff0c\u56e0\u4e3a\u5b83\u6d4b\u91cf\u7684\u662f RNA \u800c\u4e0d\u662f DNA\u3002 RT-qPCR \u80fd\u591f\u68c0\u6d4b\u4ece\u60a3\u8005\u6837\u672c\u4e2d\u7eaf\u5316\u7684 RNA \u4e2d\u76ee\u6807 COVID-19 \u5e8f\u5217\u7684\u5b58\u5728\u548c\u6c34\u5e73\uff0c\u4ece\u800c\u544a\u8bc9\u60a8\u67d0\u4eba\u662f\u5426\u88ab\u611f\u67d3\uff0c\u5982\u679c\u88ab\u611f\u67d3\uff0c\u5927\u7ea6\u611f\u67d3\u4e86\u591a\u957f\u65f6\u95f4\u3002<\/p>\n\n\n\n
\u7b2c\u4e00\u6b65\u662f\u6536\u96c6\u6837\u672c\u3002\u6536\u96c6\u53d1\u751f\u5728\u533b\u751f\u4e0b\u4ee4\u8fdb\u884c\u6d4b\u8bd5\u540e\u3002\u533b\u9662\u3001\u5b9e\u9a8c\u5ba4\u548c\u516c\u5171\u536b\u751f\u673a\u6784\u64e6\u62ed\u60a3\u8005\u7684\u9f3b\u5b50\u6216\u5634\u5df4\uff0c\u5e76\u7acb\u5373\u5c06\u6d4b\u8bd5\u62ed\u5b50\u653e\u5165\u8bd5\u7ba1\u4e2d\u3002\u7136\u540e\uff0c\u8fd9\u4e9b\u60a3\u8005\u6837\u672c\u88ab\u5b89\u5168\u5730\u5e26\u5230\u68c0\u6d4b\u5b9e\u9a8c\u5ba4\u3002<\/p>\n\n\n\n
\u4e00\u65e6\u6536\u96c6\u7ba1\u5230\u8fbe\u5b9e\u9a8c\u5ba4\uff0c\u6709\u4e09\u4e2a\u81ea\u52a8\u5316\u6b65\u9aa4\uff1a<\/p>\n\n\n\n
1.\u6837\u54c1\u63a5\u79cd2.RNA \u63d0\u53d63.qPCR \u8bbe\u7f6e<\/p>\n\n\n\n
\u6bcf\u4e2a\u6b65\u9aa4\u90fd\u6709\u6307\u5b9a\u7684 OT-2 \u5de5\u4f5c\u7ad9\uff08\u68c0\u6d4b\u9664\u5916\uff0c\u68c0\u6d4b\u5728\u5b9e\u9a8c\u5ba4\u63d0\u4f9b\u7684 qPCR \u4eea\u5668\u4e0a\u8fdb\u884c\uff09\u3002\u4ee5\u4e0b\u662f\u5982\u4f55\u5728 Opentrons \u4e0a\u5b9e\u73b0\u6d41\u7a0b\u81ea\u52a8\u5316\uff1a<\/p>\n\n\n\n
\u7ad9\u70b9 A \u2013 \u6837\u54c1\u94fa\u677f\uff1a\u5c06 96 \u4e2a\u88c5\u6709\u60ac\u6d6e\u5728\u5931\u6d3b\u7f13\u51b2\u6db2\u4e2d\u7684\u60a3\u8005\u6837\u54c1\u7684\u6536\u96c6\u7ba1\u653e\u5728\u7b2c\u4e00\u4e2a Opentrons \u673a\u5668\u4eba\u4e0a\uff0c\u91cd\u65b0\u683c\u5f0f\u5316\u4ee5\u63d0\u53d6 RNA\u3002\u673a\u5668\u4eba\u5c06\u6837\u54c1\u548c\u9633\u6027\u5bf9\u7167\u4ece\u7ba1\u4e2d\u79fb\u6db2\u5230\u6df1\u5b54\u677f\u4e2d\u3002<\/p>\n\n\n\n
\u7ad9\u70b9 A \u914d\u7f6e\uff1a9,050 \u7f8e\u5143<\/p>\n\n\n\n
p20 + p300 \u5355\u901a\u9053\u79fb\u6db2\u5668\u6e29\u5ea6\u6a21\u5757\uff08\u4e3b\u52a8\u51b7\u5374\u5bf9\u7167 RNA\uff094x \u7ba1\u67b696 \u6df1\u5b54\u63d0\u53d6\u677f1x 20ul \u67b6 + 1x 200ul \u6ee4\u82af\u5438\u5934\u67b6\u7ad9\u70b9 A \u7684 OT-2 \u7532\u677f\u5e03\u5c40\u7ad9\u70b9 A \u7684 OT-2 \u7532\u677f\u5e03\u5c40\u7ad9\u70b9 A \u5e94\u8be5\u4f4d\u4e8e\u67d0\u79cd\u201c\u8fdb\u6837\u201d\u5ba4\u3002\u6b64\u7ad9\u70b9\u7684\u4e3b\u8981\u74f6\u9888\u901a\u5e38\u4e0d\u662f\u5b9e\u9645\u7684\u91cd\u65b0\u683c\u5f0f\u5316\uff0c\u800c\u662f\u4e3a\u673a\u5668\u4eba\u8d34\u6807\u7b7e\u3001\u8bb0\u5f55\u3001\u5f00\u76d6\u548c\u5c06\u6536\u96c6\u7ba1\u88c5\u5165\u67b6\u5b50\u3002\u6700\u597d\u5c06\u6837\u54c1\u8fdb\u6837\u5ba4\u4e0e\u8fdb\u884c\u6837\u54c1\u5904\u7406\u7684\u4efb\u4f55\u7a7a\u95f4\u5206\u5f00\uff0c\u4ee5\u9632\u6b62\u6c61\u67d3\u3002\u4e00\u65e6\u6837\u672c\u548c\u9633\u6027\u5bf9\u7167 RNA \u653e\u5165\u6df1\u5b54\u677f\uff0c\u5373\u53ef\u5c06\u5176\u79fb\u81f3 B \u7ad9\u3002<\/p>\n\n\n\n
B \u7ad9 \u2013 RNA \u63d0\u53d6\uff1a96 \u4e2a\u6df1\u5b54\u677f\uff08\u5305\u542b\u6837\u672c\uff09\u88ab\u79fb\u81f3\u7b2c\u4e8c\u4e2a\u673a\u5668\u4eba\uff0c\u4f7f\u7528\u78c1\u6027\u5fae\u73e0\u8fdb\u884c RNA \u63d0\u53d6\u3002\u8fd9\u662f\u81ea\u52a8\u5316\u8fc7\u7a0b\u7684\u4e3b\u8981\u90e8\u5206\uff0c\u56e0\u4e3a\u9700\u8981\u591a\u6b21\u6e05\u6d17\u6b65\u9aa4\u624d\u80fd\u5b8c\u5168\u7eaf\u5316 RNA\u3002<\/p>\n\n\n\n
B \u7ad9\u914d\u7f6e\uff1a7,750 \u7f8e\u5143<\/p>\n\n\n\n
p300 8 \u901a\u9053\u79fb\u6db2\u5668\u78c1\u6027\u6a21\u575796 \u6df1\u5b54\u63d0\u53d6\u677f96 \u5b54\u6d17\u8131\u677f5 \u4e2a 200ul \u6ee4\u5634\u67b62 \u4e2a 12 \u901a\u9053\u8bd5\u5242\u5bb9\u56682 \u4e2a 1 \u901a\u9053\u6db2\u4f53\u5783\u573e\u5bb9\u5668<\/p>\n\n\n\n
\u8fd8\u5e94\u8be5\u6709\u4e00\u4e2a\u4e13\u95e8\u7684\u63d0\u53d6\u5ba4\uff0c\u7528\u4e8e\u5b89\u7f6e\u6240\u6709 B \u7ad9\u63d0\u53d6\u673a\u5668\u4eba\u3002\u5982\u679c\u541e\u5410\u91cf\u9700\u8981\uff0c\u8fd9\u4e2a\u623f\u95f4\u8fd8\u53ef\u4ee5\u5bb9\u7eb3\u4e00\u4e2a\u8bd5\u5242\u586b\u5145\u7ad9\uff0c\u4e3a B \u7ad9\u673a\u5668\u4eba\u51c6\u5907\u78c1\u6027\u5fae\u73e0\u548c\u7f13\u51b2\u6db2\uff0c\u4ee5\u8fdb\u884c RNA \u63d0\u53d6\u3002\u5982\u60a8\u5728\u4e0a\u9762\u7684\u7532\u677f\u56fe\u4e2d\u6240\u89c1\uff0c\u6b64\u8fc7\u7a0b\u9700\u8981\u5927\u91cf\u8bd5\u5242\u548c\u5438\u5934\u3002<\/p>\n\n\n\n
C \u7ad9 – \u68c0\u6d4b\u8bbe\u7f6e\uff1a\u4ece\u6837\u672c\u4e2d\u5206\u79bb\u51fa\u7684 RNA\uff08\u6d17\u8131\uff09\u968f\u540e\u88ab\u79fb\u81f3\u7b2c\u4e09\u4e2a\u623f\u95f4\uff0c\u8be5\u623f\u95f4\u5bb9\u7eb3 qPCR \u4eea\u5668\uff0c\u5e76\u8bbe\u8ba1\u4e3a\u5c3d\u53ef\u80fd\u201c\u5e72\u51c0\u201d\uff0c\u4ee5\u6700\u5927\u9650\u5ea6\u5730\u51cf\u5c11\u6c61\u67d3\u3002\u5c06\u88c5\u6709\u7eaf\u6837\u672c RNA \u7684\u677f\u653e\u5728 Opentrons \u673a\u5668\u4eba\u4e0a\uff0c\u4e3a RT-qPCR \u68c0\u6d4b\u505a\u51c6\u5907\u3002<\/p>\n\n\n\n
C \u7ad9 OT-2 \u914d\u7f6e\uff1a7,500 \u7f8e\u5143<\/p>\n\n\n\n
p20 \u5355\u901a\u9053Opentrons \u6e29\u5ea6\u6a21\u575796 \u5b54\u6d17\u8131\u677f96 \u5b54 PCR \u677f3x \u67b6 20ul \u8fc7\u6ee4\u5438\u5934<\/p>\n\n\n\n
RT-qPCR \u6240\u9700\u7684\u8bd5\u5242\u5206\u522b\u653e\u7f6e\u5728\u6e29\u5ea6\u6a21\u5757\u4e0a\u8fdb\u884c\u4e3b\u52a8\u51b7\u5374\u3002\u8fd9\u4e9b\u8bd5\u5242\u88ab\u7ec4\u5408\u6210\u6bcd\u6db2\u6df7\u5408\u7269\uff0c\u7136\u540e\u88ab\u5206\u914d\u5230\u677f\u4e2d\u5e76\u4e0e\u7eaf\u5316\u7684 RNA \u7ed3\u5408\u3002\u7ecf\u8fc7\u6b64\u8fc7\u7a0b\u540e\uff0c\u677f\u5c31\u53ef\u4ee5\u653e\u5165 qPCR \u673a\u5668\u4e2d\u5e76\u5206\u6790\u662f\u5426\u5b58\u5728 COVID-19\u3002<\/p>\n\n\n\n
\u541e\u5410\u91cf\u6211\u4eec\u5f00\u53d1\u4e86\u4e00\u4e2a\u7531 8 \u4e2a\u673a\u5668\u4eba\u7ec4\u6210\u7684\u7cfb\u7edf\uff082 \u4e2a A \u7ad9\u30014 \u4e2a B \u7ad9\u30012 \u4e2a C \u7ad9\uff09\uff0c\u53ef\u4ee5\u6bcf 12 \u5c0f\u65f6\u81ea\u52a8\u51c6\u5907 12 \u4e2a 96 \u5b54\u677f\uff0c\u6216\u6bcf\u5929\u5927\u7ea6 2,400 \u6b21\u6d4b\u8bd5\u3002<\/p>\n\n\n\n
8 \u4e2a\u673a\u5668\u4eba\u7cfb\u7edf\u7684\u8fc7\u7a0b\u65f6\u95f4\u5b89\u6392\u5982\u4e0b\uff1a<\/p>\n\n\n\n
\u8be5\u7cfb\u7edf\u52a0\u4e0a\u5907\u4ef6\u548c\u5b9e\u9a8c\u5ba4\u5b89\u88c5\u8d39\u7528\u4e3a 100,000 \u7f8e\u5143\uff0c\u53ef\u7528\u4e8e\u76d1\u6d4b\u9879\u76ee\u3002\u6211\u4eec\u53ef\u4ee5\u5728 3 \u6708\u4efd\u6bcf\u5468\u5b89\u88c5 1 \u6b21\uff0c\u7136\u540e\u5728 4 \u6708\u4efd\u6bcf\u5468\u5b89\u88c5 4 \u6b21\u3002\u5982\u679c\u6211\u4eec\u5b9e\u73b0\u8fd9\u4e00\u76ee\u6807\uff0c\u6211\u4eec\u6709\u671b\u5728 6 \u6708\u5e95\u4e4b\u524d\u5728 Opentrons \u7cfb\u7edf\u4e0a\u81ea\u52a8\u5b8c\u6210 100 \u4e07\u6b21\u6d4b\u8bd5\u3002<\/p>\n\n\n\n
\u6211\u4eec\u76ee\u524d\u6b63\u5728\u52aa\u529b\u5728\u52a0\u5229\u798f\u5c3c\u4e9a\u5dde\u5e15\u6d1b\u963f\u5c14\u6258\u7684\u5f00\u653e\u533b\u5b66\u7814\u7a76\u6240\u5b89\u88c5\u7b2c\u4e00\u4e2a\u9ad8\u901a\u91cf\u7cfb\u7edf\uff0c\u5e76\u6e34\u671b\u4f7f\u7528\u6211\u4eec\u7684\u673a\u5668\u4eba\u5e2e\u52a9\u5b9e\u9a8c\u5ba4\u5b8c\u6210\u8fd9\u4e2a\u5173\u952e\u9879\u76ee\u3002<\/p>\n\n\n\n
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