{"id":2828,"date":"2024-05-30T22:44:55","date_gmt":"2024-05-30T14:44:55","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2828"},"modified":"2024-09-03T14:10:16","modified_gmt":"2024-09-03T06:10:16","slug":"mxsmkxyjryd-covid-19-jcy","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/mxsmkxyjryd-covid-19-jcy\/","title":{"rendered":"\u9762\u5411\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4eba\u5458\u7684 9 \u4e2a COVID-19 \u68c0\u6d4b\u8d44\u6e90"},"content":{"rendered":"\n<p>\u51a0\u72b6\u75c5\u6bd2\u5927\u6d41\u884c\u4e3a\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u524d\u6240\u672a\u6709\u7684\u673a\u4f1a\u3002\u4ee5\u4e0b 9 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\u8ba4\u8bc1\u7684 SARS-CoV-2 \u68c0\u6d4b\u5b9e\u9a8c\u5ba4\u7684\u8be6\u5c3d\u6307\u5357\u3002IGI \u662f\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u548c\u52a0\u5dde\u5927\u5b66\u65e7\u91d1\u5c71\u5206\u6821\u7684\u7814\u7a76\u4eba\u5458\u56e2\u961f\uff0c\u4ed6\u4eec\u7ec4\u5efa\u4e86\u4e00\u652f\u591a\u5143\u5316\u3001\u8de8\u5b66\u79d1\u7684\u56e2\u961f\uff0c\u663c\u591c\u4e0d\u505c\u5730\u5de5\u4f5c\u4e86 3 \u5468\uff0c\u4ee5\u6269\u5927\u6e7e\u533a\u7684\u51a0\u72b6\u75c5\u6bd2\u68c0\u6d4b\u89c4\u6a21\u3002\u4ed6\u4eec\u7684\u6307\u5357\u5305\u62ec\u4ece\u7279\u5b9a\u81ea\u52a8\u5316\u5de5\u4f5c\u6d41\u7a0b\u548c\u534f\u8bae\u5230\u4eba\u5458\u57f9\u8bad\u548c\u5408\u89c4\u6307\u5357\u7b49\u5404\u65b9\u9762\u7684\u63d0\u793a\u3002<\/p>\n\n\n\n<p>\u5b66\u672f\u5408\u4f5c\u8bca\u65ad\u5b9e\u9a8c\u5ba4\u7684\u6807\u51c6\u64cd\u4f5c\u7a0b\u5e8f (SOP)<\/p>\n\n\n\n<p>\u514b\u91cc\u514b COVID19 \u8054\u76df\u63d0\u4f9b\u4e86\u6709\u5173\u4ed6\u4eec\u5982\u4f55\u5728\u5b66\u672f\u73af\u5883\u4e2d\u4e0e\u5916\u90e8\u5408\u4f5c\u4f19\u4f34\u4e00\u8d77\u521b\u5efa\u8bca\u65ad\u5b9e\u9a8c\u5ba4\u7684\u5206\u6b65\u6307\u5357\u3002\u514b\u91cc\u514b COVID19 \u8054\u76df\u4e0e\u4f26\u6566\u5927\u5b66\u5b66\u9662\u533b\u9662\u3001\u536b\u751f\u670d\u52a1\u5b9e\u9a8c\u5ba4 (HSL)\u3001\u764c\u75c7\u7814\u7a76\u6240\u548c\u5f17\u6717\u897f\u65af\u514b\u91cc\u514b\u7814\u7a76\u6240\u5408\u4f5c\uff0c\u5728\u77ed\u77ed\u4e24\u5468\u591a\u7684\u65f6\u95f4\u5185\u521b\u5efa\u4e86\u8fd9\u4e00\u6d4b\u8bd5\u8bbe\u7f6e\u3002\u4ed6\u4eec\u7684\u53ef\u4e0b\u8f7d\u6307\u5357\u6db5\u76d6\u4e86\u4ece\u5b9e\u9a8c\u5ba4\u5b89\u5168\u4fe1\u606f\u548c 2 \u7c7b\u6837\u54c1\u706d\u6d3b\u6307\u5357\u5230 RNA \u63d0\u53d6\u65b9\u6848\u548c\u5236\u4f5c\u81ea\u5236\u7f13\u51b2\u6db2\u7684\u6240\u6709\u5185\u5bb9\u3002<\/p>\n\n\n\n<p>\u4e34\u5e8a\u7814\u7a76\u6d4b\u5e8f\u6d4b\u8bd5\u5e73\u53f0<\/p>\n\n\n\n<p>Broad Institute \u5f00\u653e\u4e86\u6709\u5173\u5176\u9488\u5bf9 SARS-CoV-2 \u7684\u4e34\u5e8a\u7814\u7a76\u6d4b\u5e8f\u5e73\u53f0 (CRSP) \u7684\u8bbf\u95ee\u6743\u9650\u3002Broad Institute \u662f\u9ebb\u7701\u7406\u5de5\u5b66\u9662 (MIT) \u548c\u54c8\u4f5b\u5927\u5b66\u7814\u7a76\u4eba\u5458\u4e4b\u95f4\u7684\u5408\u4f5c\uff0c\u5b83\u4f7f\u7528\u4ed6\u4eec\u7684\u5e73\u53f0\u8fd0\u884c\u5b9e\u65f6\u9006\u8f6c\u5f55\u9176 (RT)-PCR \u8bca\u65ad\u68c0\u6d4b\u3002\u5bf9\u4e8e\u4efb\u4f55\u5bfb\u627e\u6d4b\u8bd5\u8bbe\u65bd\u7684\u7814\u7a76\u4eba\u5458\uff0cBroad Institute \u90fd\u5f00\u653e\u4e86\u4ed6\u4eec\u7684\u8bbe\u65bd\u3002\u4ed6\u4eec\u7684\u8bf4\u660e\u5305\u62ec\u6807\u672c\u6536\u96c6\u548c\u8fd0\u8f93\u6307\u5357\uff0c\u4ee5\u53ca\u9488\u5bf9\u60a3\u8005\u548c\u63d0\u4f9b\u8005\u7684\u8bca\u65ad\u6d4b\u8bd5\u548c\u68c0\u6d4b\u60c5\u51b5\u8bf4\u660e\u4e66\u7684\u5e38\u89c1\u95ee\u9898\u89e3\u7b54\u3002<\/p>\n\n\n\n<p>\u57fa\u56e0\u7ec4\u5de5\u7a0b\u5de5\u5177<\/p>\n\n\n\n<p>Synthego \u4e3a\u57fa\u56e0\u7ec4\u5b66\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u5de5\u5177\uff0c\u4ee5\u63d0\u9ad8\u5f00\u53d1 COVID-19 \u8bca\u65ad\u548c\u6cbb\u7597\u7684\u901f\u5ea6\u548c\u6709\u6548\u6027\u3002\u8fd9\u4e9b\u5de5\u5177\u5305\u62ec\u7528\u4e8e RNA \u6536\u96c6\u7684\u5b9a\u5236 Cas13 \u6307\u5357\u548c\u9488\u5bf9\u4e0e SARS-CoV-2 \u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684 330 \u4e2a\u4eba\u7c7b\u57fa\u56e0\u7684\u591a\u9635\u5217 CRISPR sgRNA \u57fa\u56e0\u6572\u9664\u6587\u5e93\u3002\u4ed6\u4eec\u7684\u7f51\u7ad9\u4e0a\u6709\u6240\u6709\u8fd9\u4e9b\u5de5\u5177\u7684\u94fe\u63a5\uff0c\u4ee5\u53ca\u4ed6\u4eec\u7528\u4e8e\u521b\u5efa\u8fd9\u4e9b\u5de5\u5177\u7684\u5df2\u53d1\u5e03\u6d4b\u8bd5\u6570\u636e\u3002<\/p>\n\n\n\n<p>SwabSeq \u6d4b\u8bd5\u6982\u5ff5\u9a8c\u8bc1<\/p>\n\n\n\n<p>Octant \u7684 SwabSeq NGS \u8bca\u65ad\u5de5\u4f5c\u6d41\u7a0b\u6982\u5ff5\u9a8c\u8bc1\u3002 Octant \u662f\u4e00\u5bb6\u751f\u7269\u6280\u672f\u516c\u53f8\uff0c\u4f7f\u7528\u4e13\u6709\u6280\u672f\u7ed3\u5408\u5408\u6210\u751f\u7269\u5b66\u3001\u57fa\u56e0\u7ec4\u5de5\u7a0b\u3001\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u548c\u8ba1\u7b97\u5de5\u5177\uff0c\u521b\u5efa\u4e86\u4e00\u79cd\u534f\u8bae\u6765\u6269\u5927\u57fa\u4e8e COVID19 \u5e8f\u5217\u7684\u6d4b\u8bd5\uff0c\u8be5\u534f\u8bae\u7b80\u200b\u200b\u5355\u3001\u4fbf\u5b9c\u4e14\u7075\u654f\u3002 \u5b83\u88ab\u79f0\u4e3a SwabSeq\uff0c\u4ed6\u4eec\u7684\u5b9e\u9a8c\u5ba4\u7b14\u8bb0\u6587\u6863\u5305\u62ec\u4ece\u534f\u8bae\u548c\u6d4b\u8bd5\u4ee3\u7801\u5230\u6570\u636e\u56fe\u8868\u548c\u5185\u90e8\u7b14\u8bb0\u672c\u7684\u6240\u6709\u5185\u5bb9\u3002<\/p>\n\n\n\n<p>\u5927\u89c4\u6a21\u5e76\u884c\u8bca\u65ad\u6d4b\u5b9a\u6982\u5ff5\u9a8c\u8bc1<\/p>\n\n\n\n<p>\u5bbe\u5915\u6cd5\u5c3c\u4e9a\u5dde\u7acb\u5927\u5b66\u5408\u6210\u751f\u7269\u5b66\u7814\u7a76\u5458 Howard Salis \u7684\u5927\u89c4\u6a21\u5e76\u884c NGS \u8bca\u65ad\u6d4b\u5b9a\u6982\u5ff5\u9a8c\u8bc1\u3002 Salis \u53d1\u8868\u4e86\u4e00\u4efd\u9884\u5370\u672c\uff0c\u8be6\u7ec6\u4ecb\u7ecd\u4e86\u5927\u89c4\u6a21\u5e76\u884c\u8bca\u65ad\u6d4b\u5b9a (MPDA) \u5982\u4f55\u5229\u7528\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u5728\u4e00\u6b21\u6d4b\u5b9a\u4e2d\u540c\u65f6\u8fdb\u884c\u6570\u5343\u6b21\u75c5\u6bd2 RNA \u6d4b\u91cf\uff0c\u5e76\u5728\u6bcf\u4e2a\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u5904\u7406\u591a\u8fbe 19,200 \u4e2a\u60a3\u8005\u6837\u672c\u3002\u4ed6\u7684\u9884\u5370\u672c\u8be6\u7ec6\u4ecb\u7ecd\u4e86\u4ed6\u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u7ed3\u5408\u4e86\u75c5\u6bd2 RNA \u63d0\u53d6\u3001\u4f7f\u7528\u5177\u6709\u72ec\u7279\u6761\u5f62\u7801\u7684\u60a3\u8005\u7279\u5f02\u6027\u5f15\u7269\u7684\u9006\u8f6c\u5f55\u9176 (RT) \u4ee5\u53ca\u5355\u6c60\u805a\u5408\u9176\u94fe\u53cd\u5e94 (PCR)\uff0c\u7136\u540e\u8fdb\u884c\u4e0b\u4e00\u4ee3\u6d4b\u5e8f (NGS)\u3002<\/p>\n\n\n\n<p>\u79fb\u52a8\u6d4b\u8bd5\u5b9e\u9a8c\u5ba4<\/p>\n\n\n\n<p>OpenCell \u7684\u5927\u91cf\u84dd\u56fe\u3001\u534f\u8bae\u548c\u5176\u4ed6\u8d44\u6e90\u3002OpenCell 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