{"id":2907,"date":"2024-06-13T21:04:16","date_gmt":"2024-06-13T13:04:16","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2907"},"modified":"2024-09-03T11:37:50","modified_gmt":"2024-09-03T03:37:50","slug":"zdhngswkzbdzs","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/zdhngswkzbdzs\/","title":{"rendered":"\u81ea\u52a8\u5316NGS\u6587\u5e93\u5236\u5907\u7535\u5b50\u4e66"},"content":{"rendered":"\n<p><strong>\u81ea\u52a8\u5316NGS\u6587\u5e93\u5236\u5907\u7684\u4f18\u52bf<\/strong><\/p>\n\n\n\n<p>\u6709\u4ef7\u503c\u7684 NGS \u6570\u636e\u53d6\u51b3\u4e8e\u9ad8\u8d28\u91cf\u7684\u6587\u5e93\u5236\u5907\uff1b\u800c\u6709\u6d1e\u5bdf\u529b\u7684\u6d4b\u5e8f\u5219\u4f9d\u8d56\u4e8e\u65e0\u504f\u7684\u6587\u5e93\u8986\u76d6\u548c\u826f\u597d\u7684\u4ea7\u91cf\u3002\u4e0d\u5e78\u7684\u662f\uff0c\u6587\u5e93\u5236\u5907\u8017\u65f6\u3001\u8017\u529b\u4e14\u5bb9\u6613\u51fa\u9519\u3002\u9762\u5bf9\u8fd9\u4e9b\u6311\u6218\uff0c\u9ad8\u8d28\u91cf\u7684\u6587\u5e93\u5236\u5907\u9700\u8981\u82b1\u8d39\u65f6\u95f4\u548c\u91d1\u94b1\uff0c\u4f46\u79d1\u5b66\u5bb6\u5f88\u5c11\u62e5\u6709\u5145\u8db3\u7684\u65f6\u95f4\u548c\u91d1\u94b1\u3002\u56e0\u6b64\uff0c\u7814\u7a76\u4eba\u5458\u53d1\u73b0\u4ed6\u4eec\u96c4\u5fc3\u52c3\u52c3\u7684\u76ee\u6807\u53d7\u5230\u6587\u5e93\u5236\u5907\u74f6\u9888\u7684\u9650\u5236\u3002\u73b0\u5728\uff0c\u7ecf\u8fc7\u5341\u591a\u5e74\u7684\u4f18\u5316\u548c\u6539\u8fdb\uff0c\u5b9e\u9a8c\u5ba4\u81ea\u52a8\u5316\u7684\u53d1\u5c55\u6709\u671b\u7f13\u89e3\u8fd9\u4e00\u9650\u5236\u3002\u81ea\u52a8\u5316\u4e3a NGS \u6587\u5e93\u5236\u5907\u5e26\u6765\u4e86\u8bb8\u591a\u597d\u5904\u3002\u672c\u7ae0\u5c06\u8ba8\u8bba\u4e09\u4e2a\u6700\u91cd\u8981\u7684\u597d\u5904\uff1a\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u3001\u63d0\u9ad8\u541e\u5410\u91cf\u548c\u964d\u4f4e\u6210\u672c\u3002\u6211\u4eec\u5c06\u8ba8\u8bba\u6bcf\u4e2a\u56e0\u7d20\uff0c\u7740\u773c\u4e8e\u53ef\u80fd\u8ba9\u81ea\u52a8\u5316\u65b0\u624b\u611f\u5230\u60ca\u8bb6\u7684\u65b9\u9762\uff0c\u5e76\u5f3a\u8c03\u5bf9\u7814\u7a76\u76ee\u6807\u5f71\u54cd\u6700\u5927\u7684\u65b9\u9762\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"282\" height=\"352\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-14.png\" alt=\"\u624b\u52a8\u79fb\u6db2\" class=\"wp-image-2908\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-14.png 282w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-14-240x300.png 240w\" sizes=\"(max-width: 282px) 100vw, 282px\" \/><\/figure><\/div>\n\n\n<p>\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\uff1a\u51c6\u786e\u5ea6\u548c\u7cbe\u786e\u5ea6<br>\u51c6\u786e\u5ea6\u548c\u7cbe\u786e\u5ea6\u5bf9\u4e8e\u5728\u6587\u5e93\u5236\u5907\u5de5\u4f5c\u6d41\u7a0b\u7684\u6bcf\u4e2a\u6b65\u9aa4\u4e2d\u5b9e\u73b0\u7406\u60f3\u7684\u7ed3\u679c\u81f3\u5173\u91cd\u8981\u3002\u5728\u6240\u6709\u4efb\u52a1\u4e2d\uff0c\u8bd5\u5242\u548c\u6837\u54c1\u5fc5\u987b\u4ee5\u7cbe\u786e\u7684\u91cf\u8fdb\u884c\u5206\u88c5\u3001\u7ec4\u5408\u548c\u8f6c\u79fb\uff0c\u4ee5\u9a71\u52a8\u7075\u654f\u7684\u5316\u5b66\u53cd\u5e94\u5e76\u63d0\u4f9b\u9ad8\u8d28\u91cf\u7684\u7ed3\u679c\u3002<br>\u5927\u591a\u6570\u5b9e\u9a8c\u5ba4\u5728 NGS \u6587\u5e93\u5236\u5907\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u9700\u8981\u5c06\u4f53\u79ef\u964d\u81f3 1 \u03bcl\u3002\u5728\u6b64\u4f53\u79ef\u4e0b\uff0c\u81ea\u52a8\u5316\u7cfb\u7edf\u548c\u719f\u7ec3\u7684\u4eba\u5de5\u6280\u672f\u4eba\u5458\u7684\u8868\u73b0\u76f8\u5f53\uff0c\u5b9e\u9645\u79fb\u6db2\u91cf\u5728\u76ee\u6807\u7684 5% \u4ee5\u5185\uff0c\u5373 0.95-<br>1.05 \u03bcl\uff0c\u79f0\u4e3a\u53d8\u5f02\u7cfb\u6570 (CV)\u3002<br>\u4eba\u5de5\u6280\u672f\u4eba\u5458\u548c\u81ea\u52a8\u5316\u7cfb\u7edf\u5728\u79fb\u6db2\u53d8\u5f02\u6027\u548c\u9519\u8bef\u7387\u65b9\u9762\u786e\u5b9e\u6709\u6240\u4e0d\u540c\uff0c\u5982\u4e0b\u6240\u8ff0\u3002<\/p>\n\n\n\n<p>\u51cf\u5c11\u53d8\u5f02\u6027\u548c\u9519\u8bef<br>\u81ea\u52a8\u5316\u7cfb\u7edf\u53ef\u6700\u5927\u9650\u5ea6\u5730\u51cf\u5c11\u53d8\u5f02\u6027\u5e76\u6d88\u9664\u9519\u8bef\uff0c\u800c\u6280\u672f\u4eba\u5458\u5728\u5904\u7406\u590d\u6742\u534f\u8bae\u6216\u5927\u91cf\u6837\u672c\u65f6\u53ef\u80fd\u4f1a\u9047\u5230\u8fd9\u4e9b\u65b9\u9762\u7684\u56f0\u96be\u3002\u6587\u5e93\u5236\u5907\u534f\u8bae\u53ef\u80fd\u6d89\u53ca\u6570\u5c0f\u65f6\u7684\u6f2b\u957f\u8fc7\u7a0b\u4e2d\u7684\u65e0\u6570\u4e2a\u5355\u72ec\u6b65\u9aa4\uff0c\u5e76\u4e14\u8003\u8651\u5230\u5927\u591a\u6570 NGS \u5b9e\u9a8c\u7684\u89c4\u6a21\uff0c\u79fb\u6db2\u4efb\u52a1\u5728\u6574\u4e2a\u9879\u76ee\u4e2d\u91cd\u590d\u65e0\u6570\u6b21\uff08\u6709\u5173\u66f4\u591a\u8ba8\u8bba\uff0c\u8bf7\u53c2\u9605\u7b2c\u4e8c\u7ae0\uff09\u3002<\/p>\n\n\n\n<p>\u4e0d\u53ef\u907f\u514d\u7684\u662f\uff0c\u5373\u4f7f\u662f\u719f\u7ec3\u7684\u4eba\u7c7b\u6280\u672f\u4eba\u5458\u4e5f\u4f1a\u56e0\u6ce8\u610f\u529b\u4e0d\u96c6\u4e2d\u800c\u72af\u4e0b\u79fb\u6db2\u9519\u8bef\u3002\u901a\u5e38\uff0c\u552f\u4e00\u7684\u89e3\u51b3\u65b9\u6848\u662f\u91cd\u505a\u6574\u4e2a\u7a0b\u5e8f\u3002\u7136\u800c\uff0c\u673a\u5668\u4eba\u7cfb\u7edf\u53ef\u4ee5\u4e0d\u77e5\u75b2\u5026\u5730\u3001\u5b8c\u7f8e\u5730\u6267\u884c\u79fb\u6db2\u3002\u6bcf\u6b21\u90fd\u5c06\u6b63\u786e\u7684\u6db2\u4f53\u79fb\u5230\u6b63\u786e\u7684\u5b54\u4e2d\u3002\u901a\u8fc7\u6d88\u9664\u91cd\u590d\u8fd0\u884c\uff0c\u53ef\u4ee5\u8282\u7701\u6602\u8d35\u7684\u8bd5\u5242\u548c\u5b9d\u8d35\u7684\u65f6\u95f4\uff08\u6709\u5173\u6210\u672c\u8282\u7ea6\u7684\u66f4\u591a\u4fe1\u606f\uff0c\u8bf7\u53c2\u9605\u4e0b\u6587\uff09\u3002\u6b64\u5916\uff0c\u901a\u8fc7\u6d88\u9664\u9519\u8bef\uff0c\u81ea\u52a8\u5316\u53ef\u4ee5\u63d0\u4f9b\u66f4\u53ef\u9760\u7684\u5e93\u548c\u4e0b\u6e38\u6570\u636e\u3002<\/p>\n\n\n\n<p>\u53ef\u91cd\u590d\u6027\u548c\u4e00\u81f4\u6027<br>\u5206\u6790\u9700\u8981\u5177\u6709\u53ef\u91cd\u590d\u6027\u548c\u4e00\u81f4\u6027\uff0c\u4e0d\u4ec5\u8981\u786e\u4fdd\u5176\u7ed3\u679c\u6b63\u786e\uff0c\u8fd8\u8981\u8ba9\u7814\u7a76\u4eba\u5458\u5bf9\u5176\u7ed3\u679c\u5145\u6ee1\u4fe1\u5fc3\u3002\u81ea\u52a8\u5316\u6bd4\u624b\u52a8\u6d41\u7a0b\u66f4\u597d\u5730\u652f\u6301\u5206\u6790\u7684\u53ef\u91cd\u590d\u6027\u548c\u4e00\u81f4\u6027\u3002\u5f71\u54cd\u68c0\u6d4b\u53ef\u91cd\u590d\u6027\u7684\u5173\u952e\u56e0\u7d20\u4e4b\u4e00\u662f\u79fb\u6db2\u7684\u53ef\u53d8\u6027\u3002\u81ea\u52a8\u5316\u6d88\u9664\u4e86\u5b9e\u9a8c\u8005\u6280\u672f\u7684\u53ef\u53d8\u6027\uff0c\u56e0\u4e3a\u673a\u5668\u4eba\u6bcf\u6b21\u90fd\u4ee5\u76f8\u540c\u7684\u65b9\u5f0f\u6267\u884c\u6bcf\u4e2a\u6b65\u9aa4\u3002\u6b64\u5916\uff0c\u8fd9\u652f\u6301\u66f4\u597d\u7684\u4e0b\u6e38\u5206\u6790\u3002<\/p>\n\n\n\n<p>\u5728\u66f4\u77ed\u7684\u65f6\u95f4\u5185\u5b9e\u73b0\u66f4\u9ad8\u7684\u541e\u5410\u91cf\u9664\u4e86\u63d0\u9ad8\u6570\u636e\u8d28\u91cf\u4e4b\u5916\uff0c\u81ea\u52a8\u5316\u8fd8\u53ef\u4ee5\u63d0\u9ad8\u5b9e\u9a8c\u5ba4\u7684\u541e\u5410\u91cf\uff0c\u66f4\u5feb\u5730\u751f\u6210\u66f4\u591a\u5e93\u5e76\u91ca\u653e\u5b9d\u8d35\u7684\u52a8\u624b\u65f6\u95f4\u3002\u6bcf\u4e2a\u624b\u52a8\u7a0b\u5e8f\u9700\u8981 3-4 \u5c0f\u65f6\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0c\u81ea\u52a8\u5316\u7cfb\u7edf\u53ef\u4ee5\u5728\u4e0d\u5230 3 \u5c0f\u65f6\u5185\u5b8c\u6210\u4e00\u4e2a\u5e93\uff0c\u52a8\u624b\u65f6\u95f4\u4e0d\u5230 15 \u5206\u949f\uff0c\u800c\u4e14\u81ea\u52a8\u5316\u7cfb\u7edf\u53ef\u4ee5\u5728\u4e0b\u73ed\u65f6\u95f4\u5de5\u4f5c\u3002\u51ed\u501f\u66f4\u5feb\u7684\u5468\u8f6c\u65f6\u95f4\uff0c\u81ea\u52a8\u5316\u53ef\u4ee5\u7f29\u77ed\u5b9e\u9a8c\u65f6\u95f4\u5e76\u66f4\u5feb\u5730\u63d0\u4f9b\u6709\u5f71\u54cd\u529b\u7684\u6570\u636e\u3002\u51ed\u501f\u66f4\u9ad8\u7684\u541e\u5410\u91cf\uff0c\u81ea\u52a8\u5316\u53ef\u4ee5\u4fc3\u8fdb\u66f4\u96c4\u5fc3\u52c3\u52c3\u7684\u5b9e\u9a8c\u5e76\u4e3a\u5b9e\u9a8c\u8bbe\u8ba1\u5f00\u8f9f\u65b0\u7684\u9014\u5f84\u3002<\/p>\n\n\n\n<p>\u7531\u4e8e\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u51cf\u5c11\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5c06\u66f4\u591a\u7cbe\u529b\u6295\u5165\u5230\u5b9e\u9a8c\u8bbe\u8ba1\u548c\u6570\u636e\u5206\u6790\u4e0a\uff0c\u4ece\u800c\u5145\u5206\u53d1\u6325\u5176\u5b9d\u8d35\u7684\u6280\u80fd\u3002<\/p>\n\n\n\n<p>\u964d\u4f4e\u6210\u672c<br>\u81ea\u52a8\u5316\u7cfb\u7edf\u53ef\u80fd\u9700\u8981\u5927\u91cf\u7684\u521d\u59cb\u6295\u8d44\uff0c\u5c3d\u7ba1\u4e00\u4e9b\u73b0\u4ee3\u7cfb\u7edf\u66f4\u4fbf\u5b9c\uff08\u8bf7\u53c2\u9605\u7b2c 3 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