{"id":2911,"date":"2024-06-14T22:00:22","date_gmt":"2024-06-14T14:00:22","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2911"},"modified":"2024-09-03T11:14:49","modified_gmt":"2024-09-03T03:14:49","slug":"ot-2hbsthp-1xbgyhqspdzdcd","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/ot-2hbsthp-1xbgyhqspdzdcd\/","title":{"rendered":"OT-2\u6d77\u62a5\u4e0aTHP-1\u7ec6\u80de\u8fc7\u6c27\u5316\u6c22\u6c34\u5e73\u7684\u81ea\u52a8\u6d4b\u5b9a"},"content":{"rendered":"\n
\u6d4b\u5b9aOT-2\u4e0aTHP-1\u7ec6\u80de\u4e2d\u8fc7\u6c27\u5316\u6c22\u6c34\u5e73\u7684\u81ea\u52a8\u5316\u5206\u6790<\/p>\n\n\n\n
\u74e6\u82cf\u8fbeSNair\u535a\u58eb. 1\u91d1\u7eb3\u91cc\u00b7\u6c83\u68ee\u535a\u58eb11.Optrins\u5b9e\u9a8c\u5ba4\u516c\u53f8\u3002<\/p>\n\n\n\n
\u4ecb\u7ecd<\/strong><\/p>\n\n\n\n \u6c99\u6842\u6cb9\u8fc7\u6c27\u5316\u6c22\uff08H2O2)\u4e3b\u8981\u4e0e\u7ec6\u80de\u635f\u4f24\u6709\u5173\uff1b\u7136\u800c\uff0c\u91cd\u65b0\u751f\u6210\u767e\u7f8e\u5206\u7684\u7814\u7a76\u8868\u660e\uff0c\u7ec6\u80de\u91ca\u653e\u4f4e\u6c34\u5e73\u7684H2O2\u4f5c\u4e3a\u6b63\u5e38\u7ec6\u80de\u95f4\u7684\u4e00\u90e8\u5206\u9ad8\u9891\u7387\u591a\u8def\u901a\u4fe1\u8fc7\u6c27\u5316\u6c22\u7684\u8f6c\u8fd0\u3001\u6444\u53d6\u548c\u91ca\u653e\u7684\u673a\u5236\u751f\u7269\u5b66\u6548\u5e94\u76ee\u524d\u8fd8\u4e0d\u4e3a\u4eba\u6240\u77e5\uff0c\u4f46\u5bf9\u764c\u75c7\u3001\u5e72\u7ec6\u80de\u6709\u91cd\u8981\u610f\u4e49\u7ec6\u80de\u548c\u8870\u8001\u3002 \u6750\u6599\u548c\u65b9\u6cd5<\/strong><\/p>\n\n\n\n OT-2\uff0cP300\u5355GEN2\u79fb\u6db2\u7ba1\uff0cP20\u5355GEN2\u79fb\u6db2\u7ba1
\u53cc\u8054\u4f53\u7ea2\u8272\u8bd5\u5242\u662f\u975e\u8367\u5149\u7684\u57fa\u4e8e10-\u4e59\u91703,7\u4e8c\u7f9f\u57fa\u82ef\u6c27\u6076\u55ea\u7684\u6c27\u5316\uff0c\u6d4b\u5b9a\u539f\u7406\u7b80\u5355\u5b83\u662f\u7531\u8fa3\u6839\u8fc7\u6c27\u5316\u7269\u9176\uff08HRP\uff09\u50ac\u5316\u7684\uff0c\u5728H2O2\u4ea7\u751f\u4e00\u4e2a\u7ea2\u8272\u8367\u5149\u4ea7\u7269\uff0c\u95f4\u82ef\u4e8c\u915a\uff0c\u5316\u5b66\u8ba1\u91cf\u6bd4\u4e3a1\uff1a1\u3002
\u6811\u8102\u6700\u7ec8\u4ea7\u7269\u5177\u6709\u8f83\u9ad8\u7684\u6d88\u5149\u7cfb\u6570\u8fd9\u662f\u4e00\u79cd\u8d85\u7075\u654f\u7684\u68c0\u6d4b\u65b9\u6cd5\uff0c\u53ea\u670910\u76ae\u6469\u5c14\u7684H2O2\u5728\u4e00\u4e2a100 \u03bcL\u7684\u5377\u4e2d\uff1b1\u00d710\u20135U\/mL\u7684HRP\u3002
\u8fd9\u4e9b\u8bfb\u6570\u53ef\u4ee5\u7528\u5206\u5149\u5149\u5ea6\u6cd5\u6d4b\u91cf\uff0c\u4e5f\u53ef\u4ee5\u7528\u8367\u5149\u8bfb\u6570\u6cd5\u6d4b\u91cf\u4f7f\u7528\u9176\u6807\u4eea\uff0c\u6fc0\u53d1530 nm\uff0c\u53d1\u5c04\u6ce2590 nm\u3002<\/p>\n\n\n\n
\u6e29\u5ea6\u6a21\u5757\u8bbe\u7f6e\u4e3a23\u00b0C\uff0c\u5728\u534f\u8bae\u7ed3\u675f\u65f6\u8bbe\u7f6e\u4e3a37\u00b0C\u3002
THP-1\u5de8\u566c\u7ec6\u80de\u6837\u7ec6\u80de\uff08ATCC\uff0cManassas\uff0cVA\uff0cUSA\uff09(No\u3002TIB-202)
\u65e0\u915a\u7ea2RPMI 1640\u57f9\u517b\u57fa\uff08\u70ed\u8d39\u96ea\u79d1\u5b6611835030\uff09
\u4f5b\u6ce212-\u8089\u8c46\u853b\u917813-\u918b\u9178\u916f\uff08PMA\uff09\uff08Sigma Aldrich P1585-1MG\uff09
\u80ce\u725b\u8840\u6e05\uff08\u80ce\u725b\u8840\u6e05\uff09\u672a\u70ed\u706d\u6d3b\uff08ATCC\uff0cCat No. 30-2020\uff09
Amplex\u7ea2\u8272\u68c0\u6d4b\u8bd5\u5242\u76d2(Cat No.A22188)\uff0c\u5305\u62eca) 5X\u53cd\u5e94\u7f13\u51b2\u6db2b) HRP c) DMSO d) Amplex\u7ea2\u8272\u8bd5\u5242e) 3% H2O2
a)\u89c6\u4e2d\u5fc396\u8fc7\u6ee4\u5668\u5c16\u7aef\u67b6200\u03bcL b)96\u8fc7\u6ee4\u5668\u5c16\u7aef\u67b620\u03bcL c)\u89c6\u4e2d\u5fc310\u7ba1\u67b6\u4e0e\u730e\u9e704 x 50 mL\uff0c6 x15mL\u9525\u5f62d)\u89c6\u4e2d\u5fc324\u7ba1\u67b6\u4e0e\u57c3\u5f6d\u591a\u592b1.5 mL\u5b89\u5168\u9501\u6263\u5e3d\uff08\u4e24\u4e2a\uff09<\/p>\n\n\n\n