{"id":3073,"date":"2024-06-29T11:09:23","date_gmt":"2024-06-29T03:09:23","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=3073"},"modified":"2024-09-04T18:00:23","modified_gmt":"2024-09-04T10:00:23","slug":"shiyong-ot-2-zdhyypt","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/knowledge2\/shiyong-ot-2-zdhyypt\/","title":{"rendered":"\u4f7f\u7528 OT-2 \u81ea\u52a8\u5316\u79fb\u6db2\u5e73\u53f0\u8fdb\u884c\u9ad8\u6548\u6838\u9178\u63d0\u53d6"},"content":{"rendered":"\n<p>\u5e94\u7528\u6982\u8ff0<br>\u968f\u7740\u4e0d\u65ad\u8fdb\u6b65\u7684\u6280\u672f\u63a8\u52a8\uff0c\u73b0\u4ee3\u5206\u5b50\u751f\u7269\u5b66\u6301\u7eed\u53d6\u5f97\u4e07\u4f17\u77a9\u76ee\u7684 \u7a81\u7834\u548c\u53d1\u5c55\uff0c\u5305\u62ec\u66f4\u5927\u89c4\u6a21\u7684\u7814\u7a76\u3001\u66f4\u5f3a\u5927\u7684\u6cbb\u7597\u65b9\u6cd5\u4ee5\u53ca\u5168\u65b0 \u7684\u7814\u7a76\u95ee\u9898\u3002\u7136\u800c\uff0c\u5c3d\u7ba1\u6838\u9178\u63d0\u53d6\u8fd9\u9879\u6280\u672f\u662f\u5206\u5b50\u751f\u7269\u5b66\u5f97\u4ee5\u8fdb \u6b65\u7684\u4e00\u9879\u91cd\u8981\u4e14\u5bc6\u4e0d\u53ef\u5206\u7684\u5b9e\u9a8c\u73af\u8282\uff0c\u79d1\u7814\u4eba\u5458\u4ecd\u7136\u5728\u6cbf\u7528\u7740\u51e0 \u5341\u5e74\u524d\u5197\u957f\u800c\u7e41\u7410\u7684\u7a0b\u5e8f\u8fdb\u884c\u6838\u9178\u63d0\u53d6\u3002\u5728\u8fd9\u6837\u7684\u56f0\u5c40\u4e4b\u4e0b\uff0c\u81ea \u52a8\u5316\u89e3\u51b3\u65b9\u6848\u5e94\u8fd0\u800c\u751f\u3002\u73b0\u4ee3\u81ea\u52a8\u5316\u901a\u8fc7\u5f15\u5165\u5177\u5907\u7a33\u5b9a\u6027\u548c\u9ad8\u6548 \u8fd0\u4f5c\u4f18\u52bf\u7684\u673a\u5668\u8bbe\u5907\u5b8c\u6210\u6807\u51c6\u5316\u6d41\u7a0b\u9ad8\u6548\u81ea\u52a8\u8fd0\u884c\uff0c\u81ea\u52a8\u5316\u6838\u9178 \u63d0\u53d6\u5c06\u4f7f\u751f\u7269\u5b9e\u9a8c\u5ba4\u65e7\u6709\u7684\u5de5\u4f5c\u65b9\u5f0f\u8fce\u6765\u73b0\u4ee3\u5316\u3002\u4e3a\u4e86\u771f\u6b63\u610f\u4e49 \u4e0a\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u8fbe\u5230\u9ad8\u6548\u7814\u53d1\u6216\u751f\u4ea7\u7684\u76ee\u7684\uff0c\u81ea\u52a8\u5316\u7cfb\u7edf\u9700\u8981\u8fbe \u5230\u751a\u81f3\u8d85\u8d8a\u9ad8\u6280\u80fd\u6280\u672f\u4eba\u5458\u7684\u8868\u73b0\uff0c\u4e3a\u4e0b\u6e38\u5de5\u4f5c\u6d41\u7a0b\u63d0\u4f9b\u9ad8\u8d28\u91cf\u7684\u6837\u672c\u3002<\/p>\n\n\n\n<p>\u672c\u9879\u7814\u7a76\u65e8\u5728\u6d4b\u8bd5Opentrons OT-2\u6838\u9178\u5de5\u4f5c\u7ad9\u4f5c\u4e3a\u6709\u6548\u81ea\u52a8\u5316 \u78c1\u73e0\u57fa\u56e0\u63d0\u53d6\u5de5\u5177\u7684\u6027\u80fd\u3002\u7814\u7a76\u4e2d\u4f7f\u7528\u4e86\u51e0\u79cd\u7814\u7a76\u4eba\u5458\u548c\u4e34\u5e8a\u533b \u751f\u5e38\u7528\u7684\u5e38\u89c1\u6a21\u677f\uff1a\u4eba\u7c7b\u553e\u6db2\u3001\u4eba\u7c7b\u53e3\u8154\u62ed\u5b50\u3001\u7ec6\u83cc\u57f9\u517b\u7269\u548c RNA \u75c5\u6bd2\u3002\u540c\u65f6\uff0c\u4f7f\u7528\u4e86\u884c\u4e1a\u4e2d\u5e38\u89c1\u7684\u4e3b\u6d41\u8bd5\u5242\u54c1\u724c\u3002\u901a\u8fc7\u4ea7 \u91cf\u3001CV \u503c\u548cqPCR \u6570\u636e\u8fdb\u884c\u6027\u80fd\u8bc4\u4f30\uff0c\u5b9e\u9a8c\u6570\u636e\u5c06\u4e0e\u9ad8\u6280\u80fd\u6280\u672f\u4eba\u5458\u7684\u624b\u52a8\u64cd\u4f5c\u7ed3\u679c\u8fdb\u884c\u6bd4\u8f83\u3002<\/p>\n\n\n\n<p>\u7ecf\u8fc7\u7814\u7a76\u6d4b\u8bd5\uff0cOT-2 \u8868\u73b0\u4f18\u5f02\u3002\u4f7f\u7528OT-2 \u8fdb\u884c\u63d0\u53d6\u7684\u5b9e\u9a8c\u53d8\u5f02 \u6027\u964d\u4f4e\uff0c\u540c\u65f6\u590d\u73b0\u7387\u63d0\u9ad8\uff0c\u5e76\u4e14\u80fd\u5728\u8f83\u4f4e\u7684\u6210\u672c\u548c\u66f4\u5feb\u7684\u5468\u8f6c\u65f6 \u95f4\u4e0b\u63d0\u4f9b\u4e86\u7c7b\u4f3c\u7684\u4ea7\u91cf\u3002\u91cd\u8981\u7684\u662f\uff0c\u8fd9\u4e9b\u6027\u80fd\u6539\u8fdb\u548c\u6210\u672c\u8282\u7701\u53ef \u4ee5\u968f\u7740\u541e\u5410\u91cf\u7684\u589e\u52a0\u800c\u63d0\u5347\u3002\u56e0\u6b64\uff0c\u8be5\u6d4b\u8bd5\u8868\u660e\uff0c\u4f7f\u7528 OT-2 \u8fdb \u884c\u6838\u9178\u63d0\u53d6\u81ea\u52a8\u5316\u53ef\u4ee5\u4f7f\u5b9e\u9a8c\u6d41\u7a0b\u9ad8\u6548\u81ea\u52a8\u5316\uff0c\u8ba9\u81ea\u52a8\u5316\u6838\u9178\u63d0\u53d6\u80fd\u591f\u6ee1\u8db3\u9ad8\u7aef\u5206\u5b50\u751f\u7269\u5b66\u7684\u9ad8\u6027\u80fd\u6807\u51c6\u3002<\/p>\n\n\n\n<p>\u7814\u7a76\u6750\u6599\u548c\u65b9\u6cd5<br>\u4eba\u7c7b\u553e\u6db2\uff1a \u4ece\u533f\u540d\u6350\u8d60\u8005\u5904\u6536\u96c6\u65b0\u9c9c\u553e\u6db2\u5e76\u50a8\u5b58\u572815 mL \u9525\u5f62\u7ba1\u4e2d\u76f4\u81f3\u4f7f\u7528\u3002<\/p>\n\n\n\n<p>\u53e3\u8154\u62ed\u5b50\uff1a\u4f7f\u752820 mm break point (Zymo@) \u53e3\u8154\u91c7\u96c6\u62ed\u5b50\u5bf9\u533f\u540d \u5fd7\u613f\u8005\u8fdb\u884c\u91c7\u6837\uff0c\u5e76\u50a8\u5b58\u4e8e\u57281 mL Zymo DNA\/RNA \u4fdd\u62a4\u6db2\u4e2d\uff0c\u5ba4\u6e29\u4fdd\u5b58\u8fd0\u8f93\uff0c<\/p>\n\n\n\n<p>\u7ec6\u83cc\u57f9\u517b\uff1a \u4f7f\u7528 One shot\u00b0TOP10 \u5236\u5907\u611f\u53d7\u6001\u5927\u80a0\u6746\u83cc (Thermo Fisher Scientific*) \u572837\u00b0\u2103,225 rpm \u632f\u8361\u6761\u4ef6\u4e0b\u5728\u6eb6\u83cc\u6db2\u4e2d\u57f9\u517b\u8fc7 \u591c\u3002\u4f7f\u7528\u524d\uff0c\u6bcf\u4e2a\u6837\u54c1\u75282000 rpm \u79bb\u5fc31.5 \u5206\u949f\u3002\u53bb\u9664\u4e0a\u6e05\u6db2\uff0c\u6c89\u6dc0\u7269\u88ab\u91cd\u65b0\u60ac\u6d6e\u4e8e\u7b49\u4f53\u79ef\u7684\u51b7\u78f7\u9178\u76d0\u7f13\u51b2\u6db2\u4e2d\u3002\u6b64\u5916\uff0c\u5bf9\u4e8e\u4e73\u9178\u6746\u83cc\u6837\u54c1\uff0c\u6bcf\u4e2a\u6837\u54c1\u4f7f\u7528\u7ea61\u00d7107\u62f7\u8d1d\/\u03bcL\u7684 Lactobacillus plantarum(ATCC\u00b08014MINIPACKTM)\u3002<\/p>\n\n\n\n<p>\u75c5\u6bd2\uff1a\u7531\u4e8e2020\u5e743\u6708COVID-19 \u5168\u7403\u5927\u6d41\u884c\u5f00\u59cb(1),\u75c5\u6bd2 RNA \u8bd5\u5242\u76d2\u4e2d\u4f7f\u7528\u4e86\u7684\u5408\u6210 SARS-COV-2 RNA Control 2(Twist Biosci- ences), \u6bcf\u4e2a\u6837\u54c110\u62f7\u8d1d\/\u03bcL\u3002<\/p>\n\n\n\n<p>\u5185\u90e8\u5bf9\u7167\uff1a\u6309\u7167 Panpradist\u3001Nuttada \u7b49\u4eba(2)\u4e2d\u6240\u8ff0\u4f7f\u7528\u548c\u521b\u5efa\u5408 \u6210\u9f3b\u6837\u57fa\u8d28(SNM)\u3002SNM \u7684\u914d\u65b9\u4e3a\uff1a110 mM NaCl\u30011%w\/v\u6765\u81ea\u732a \u80c3\u7c7b\u578bI\u7684\u7c98\u86cb\u767d(Sigma M2378-100G)\u548c10 \u03bcg\/mL w\/v \u4eba\u7c7b\u57fa \u56e0\u7ec4DNA(Coriell NA12878), \u6df7\u5408\u4f53\u79ef\u4e3aTE\/SNM\u768490%\u3002\u6bcf\u4e2a\u75c5\u6bd2\u6837\u672c\u4f7f\u752850 pg\/\u03bcL\u7684 SNM\u3002<\/p>\n\n\n\n<p>\u8bd5\u5242\u76d2\uff1a\u672c\u9879\u7814\u7a76\u4e2d\u9009\u62e9\u4e86\u6765\u81ea\u4e0d\u540c\u54c1\u724c\u7684 DNA\u3001RNA \u548c\u75c5\u6bd2 RNA\u63d0\u53d6\u8bd5\u5242\u76d2(\u53c2\u89c1\u88681)\u3002\u603b\u5171\u68c0\u9a8c\u4e867\u4e2a\u8bd5\u5242\u54c1\u724c\u768413\u79cd\u8bd5\u5242\u76d2\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"677\" height=\"301\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-122.png\" alt=\"\u672c\u7814\u7a76\u6240\u68c0\u9a8c\u7684\u63d0\u53d6\u8bd5\u5242\" class=\"wp-image-3074\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-122.png 677w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-122-300x133.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-122-576x256.png 576w\" sizes=\"(max-width: 677px) 100vw, 677px\" \/><figcaption class=\"wp-element-caption\"><strong>\u88681:\u672c\u7814\u7a76\u6240\u68c0\u9a8c\u7684\u63d0\u53d6\u8bd5\u5242\u3002<\/strong>\u5728\u4e03\u4e2a\u77e5\u540d\u8bd5\u5242\u54c1\u724c\u4e2d\uff0c\u6709\u4e24\u79cdDNA\u63d0\u53d6\u8bd5\u5242\u76d2\u3001\u56db\u79cdRNA\u63d0\u53d6\u8bd5\u5242\u76d2\u548c\u4e03\u79cd\u75c5\u6bd2RNA\u63d0\u53d6\u8bd5\u5242\u76d2\uff0c\u5747\u53ef\u5728OT-2\u4e0a\u8fdb\u884c\u81ea\u52a8\u5316\u64cd\u4f5c\u3002<\/figcaption><\/figure>\n\n\n\n<p>\u63d0\u53d6\uff1a \u5728\u81ea\u52a8\u5316\u68c0\u6d4b\u4e4b\u524d\uff0c\u6211\u4eec\u5bf9\u6bcf\u4e2a DNA \u548c RNA \u63d0\u53d6\u8bd5\u5242\u53ca \u6a21\u677f\u8fdb\u884c\u4e86\u624b\u52a8\u6d4b\u8bd5\uff0c\u4ee5\u83b7\u53d6\u624b\u52a8\u6d4b\u8bd5\u57fa\u7ebf\u6d53\u5ea6\u3002\u6bcf\u4e2a\u6a21\u677f\u5728\u6bcf \u79cd\u8bd5\u5242\u4e2d\u8fdb\u884c7\u6b21\u91cd\u590d\u6d4b\u8bd5\uff0c\u5e76\u914d\u59071 \u4e2a\u9634\u6027\u5bf9\u7167\u6837\u672c\u3002\u672c\u6b21\u7814 \u7a76\u5728 Opentrons \u5e73\u53f0\u4e0a\u8fdb\u884c\u4e86\u81ea\u52a8\u5316\u6838\u9178\u63d0\u53d6\u3002\u5bf9\u4e8e DNA \u548c RNA \u63d0\u53d6\u8bd5\u5242\uff0c\u6bcf\u4e2a\u6837\u672c\u8fdb\u884c\u4e867 \u6b21\u63d0\u53d6\u3002\u5bf9\u4e8e\u75c5\u6bd2RNA \u63d0\u53d6\u8bd5 \u5242\u76d2\uff0c\u5305\u62ec\u4e8622 \u4e2a\u5e26\u6709\u76f8\u540c\u7684 SARS-CoV-2 \u548c SNM \u6a21\u677f\u7684\u6837 \u672c\u3002\u6bcf\u4e2a OT-2 \u8fd0\u884c\u4e2d\u90fd\u5305\u62ec1\u4e2a\u9634\u6027\u5bf9\u7167\u6837\u672c(nuclease-free H2O) \u3002OT-2 \u7684\u8bbe\u5907\u88c5\u914d\u4e86 GEN2 \u78c1\u73e0\u7eaf\u5316\u6a21\u5757\u3001p300 \u591a\u901a\u9053 \u79fb\u6db2\u5668\u548c\u53ef\u9009\u914d\u7684 GEN2 \u6e29\u63a7\u6a21\u5757\uff0c\u5bf9\u4e8e RNA \u5b9e\u9a8c\u6765\u8bf4\uff0c\u4e3a\u4fdd\u8bc1 \u66f4\u597d\u7684\u5b9e\u9a8c\u6548\u679c\uff0c\u5efa\u8bae\u914d\u5907\u6e29\u63a7\u6a21\u5757\u3002\u6839\u636e\u4e0d\u540c\u7684\u8bd5\u5242\u76d2\uff0c\u9700\u8981 \u4f7f\u7528\u5927\u698214\u76d2300 \u03bcL \u5438\u5934\uff0c1 \u4e2a NEST \u7684\u5355\u5b54\u50a8\u6db2\u5668\uff0c1 \u4e2aNEST 2 mL\u6df1\u5b54\u677f\uff0c2\u4e2a NEST12 \u5b54\u50a8\u6db2\u69fd\u548c1\u4e2aPCR \u5b54\u677f\u3002<\/p>\n\n\n\n<p>\u5f15\u7269\uff1a\u75be\u75c5\u63a7\u5236\u4e0e\u9884\u9632\u4e2d\u5fc3(CDC)\u7684\u5f15\u7269\u548c\u63a2\u9488\uff1a<\/p>\n\n\n\n<p>2019-nCoV_N1-F(GACCCCAAAATCAGCGAAAT)<br>2019-nCoV_N1-R (TCTGGTTACTGCCAGTTGAATCTG)<br>2019-nCoV_N1-P(FAM-<br>ACCCCGCATTACGTTTGGTGGACC-BHQ1)<br>2019-nCoV_N2-F(TTACAAACATTGGCCGCAAA)<br>2019-nCoV_N2-R(GCGCGACATTCCGAAGAA)<br>2019-nCoV_N2-P(FAMACAATTTGCCCCCAGCGCTTCAG-BHQ1)<br>RP-F(AGATTTGGACCTGCGAGCG),<br>RP-R(GAGCGGCTGTCTCCACAAGT)<br>RP-P (FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ1)<\/p>\n\n\n\n<p>CDC \u5f15\u7269\u548c\u63a2\u9488\u8ba2\u8d2d\u81ea\u4eceIntegrated DNA Technologies\u516c\u53f8\uff0c\u53c8\u540dIDT (2019-nCoV CDC EUA\u8bd5\u5242\u76d2)\u3002N1\u3001N2 \u548cRNase P\u4ee5CDC \u63a8\u8350\u7684\u6d53\u5ea6\u8fdb\u884c\u9884\u6df7\u3002<br>16s \u5f15\u7269\uff1a<br>Forward (CCTATAAGACTGGGATAACTTCGGG)<br>Reverse (CTTTGAGTTTCAACCTTGCGGTCG)<br>16s \u5f15\u7269\u4eceIDT \u8ba2\u8d2d\uff0c\u4e8eIDTE 1XTE \u6eb6\u6db2 pH:8.0(DT) \u4e2d\u91cd\u60ac\uff0c\u7a00\u91ca\u81f310uM \u7684\u5de5\u4f5c\u6d53\u5ea6\u3002<\/p>\n\n\n\n<p>\u5b9a\u91cf\u6d4b\u91cf\uff1a DNA \u548c RNA \u6837\u672c\u7684\u6d53\u5ea6\u53ef\u4ee5\u4f7f\u7528 Qubit 4.0 FluorometerM(Thermo Fisher)\u8fdb\u884c\u5b9a\u91cf\u6d4b\u91cf\u3002<\/p>\n\n\n\n<p>\u6b64\u5916\uff0c\u6240\u6709\u6837\u54c1\u5747\u5728 PCR max ECO48 \u5b9e\u65f6 PCR \u7cfb\u7edf\u4e0a\u8fdb\u884c qPCR \u5b9a \u91cf\u3002RNA \u6837\u54c1\u4f7f\u7528Luna\u00b0\u901a\u7528\u63a2\u9488\u4e00\u6b65\u5f0f RT-qPCR \u8bd5\u5242\u76d2(NEB) \u8fdb\u884c \u6d4b\u8bd5\uff0c\u6d53\u5ea6\u7531 Lista \u3001Maria Jose \u7b49\u4eba\u6d4b\u8bd5(3)\u3002 \u8fdb\u884c\u4ee5\u4e0b\u7a0b\u5e8f\uff1a\u5728 55\u2103\u4e0b\u8fdb\u884c\u9006\u8f6c\u5f5510\u5206\u949f\u3002\u521d\u59cb\u53d8\u6027\u572895\u2103\u4e0b\u8fdb\u884c1 \u5206\u949f\uff0c\u7136\u540e\u8fdb \u884c50\u4e2a\u5faa\u73af\uff0c\u5373\u572895\u2103\u4e0b\u53d8\u602710\u79d2\uff0c\u5e76\u572860\u2103\u4e0b\u9000\u706b30\u79d2\u3002\u4f7f\u7528 Luna Universal qPCR Master Mix (NEB)\u4ee5\u5236\u9020\u5546\u63a8\u8350\u7684\u6d53\u5ea6\u5bf9DNA\u6837 \u54c1\u8fdb\u884c\u6d4b\u8bd5\u3002\u4f7f\u7528\u4ee5\u4e0b\u7a0b\u5e8f\uff1a\u521d\u59cb\u53d8\u6027\u572895\u2103\u4e0b\u8fdb\u884c60\u79d2\uff0c\u968f\u540e\u53d8\u6027\u548c\u5ef6\u4f38\u5206\u522b\u572895\u2103\u548c60\u2103\u4e0b\u8fdb\u884c15\u79d2\u548c30\u79d2\uff0c\u6301\u7eed40\u4e2a\u5faa\u73af\u3002<\/p>\n\n\n\n<p>\u5b9e\u9a8c\u7ed3\u679c<br>\u901a\u8fc7 qPCR \u548c\u8367\u5149\u8ba1\u5206\u6790\u6d4b\u91cf\uff0c OT-2 \u63d0\u53d6\u7684\u6838\u9178\u6570\u91cf\u63a5 \u8fd1\uff0c\u6838\u9178\u6837\u54c1\u7684 \u4e00 \u81f4\u6027\u66f4\u9ad8\u3002<\/p>\n\n\n\n<p>OT-2 \u7ecf\u8fc7\u4e86\u7b26\u5408EUA \u6807 \u51c6 \u7684\u6027\u80fd\u6d4b\u8bd5\uff0c\u53ef\u4ee5\u4e0e\u591a\u79cdqPCR \u6838\u63d0\u53d6\u8bd5\u5242\u76d2 \u4e00 \u8d77\u4f7f\u7528\u3002<\/p>\n\n\n\n<p>\u4f7f\u7528\u4e5d\u79cd\u5e38\u7528\u7684\u63d0\u53d6\u8bd5\u5242\u76d2\u5904\u7406\u4eba\u7c7b\u553e\u6db2\u548c\u690d\u7269\u4e73\u6746\u83cc\u7ec6\u83cc\u57f9\u517b \u7269\uff0c\u5e76\u4f7f\u7528\u4e24\u79cd\u5e38\u89c1\u7684 qPCR \u9776\u6807\u8fdb\u884c qPCR \u5206\u6790\uff1a RNase P \u7528\u4e8e \u553e\u6db2\u6837\u672c\uff0c16s \u7528\u4e8e\u7ec6\u83cc\u6837\u672c\u3002\u4f7f\u7528\u516d\u4e2a\u8bd5\u5242\u76d2\u5904\u7406\u5927\u80a0\u6746\u83cc\u57f9\u517b\u7269\u5e76\u4f7f\u7528\u8367\u5149\u5206\u6790\u8fdb\u884c\u5206\u6790\u3002<\/p>\n\n\n\n<p>\u4ea7\u91cf\u901a\u8fc7qPCR \u4e2d\u7684\u5faa\u73af\u9608\u503c(Ct) \u548c\u8367\u5149\u8ba1\u4e2d\u6240\u5f97\u7684ng\/\u03bcL\u8fdb\u884c\u8bc4\u4f30\u3002 \u4e00\u81f4\u6027\u5219\u901a\u8fc7Ct\u503c\u7684\u53d8\u5f02\u7cfb\u6570 (CV) \u8fdb\u884c\u8bc4\u4f30\u3002<\/p>\n\n\n\n<p>Ct\u503c\u53cd\u6620\u4e86\u6837\u54c1\u8de8\u8fc7\u9ad8\u4e8e\u80cc\u666f\u4fe1\u53f7\u7684\u9608\u503c\u6240\u9700\u7684\u590d\u5236\u5468\u671f\u91cf\uff0c\u56e0\u6b64 \u5b83\u4eec\u4f20\u8fbe\u4e86\u6570\u91cf\u7684\u5012\u6570\u6d4b\u91cf\u3002\u5bf9\u4e8e\u8fd9\u4e9b\u76ee\u6807\uff0c\u5728\u6b64\u7c7b\u6837\u54c1\u4e2d\uff0c25-35\u7684 Ct \u503c\u8f83\u4e3a\u53ef\u9760\u3002<\/p>\n\n\n\n<p>\u901a\u8fc7\u6240\u6709\u63d0\u53d6\u8bd5\u5242\u76d2\u8fdb\u884c\u68c0\u9a8c\u6d4b\u8bd5\uff0c\u4f7f\u7528OT-2\u81ea\u52a8\u5316\u7cfb\u7edf\u8fdb\u884c\u6838\u9178 \u63d0\u53d6\u7eaf\u5316\u53ef\u4ee5\u5f97\u5230\u5177\u6709\u8f83\u4f4eCV \u548c\u53ef\u6bd4\u4ea7\u91cf\u7684Ct\u503c(\u89c1\u56fe1\u548c\u88682)\u3002\u719f\u7ec3\u7684\u6280\u672f\u4eba\u5458\u548cOT-2\u8fd4\u56de\u7684\u5e73\u5747Ct\u503c\u4e3a25-35\u3002<\/p>\n\n\n\n<p>\u624b\u52a8\u5904\u7406\u7684CV \u503c\u4e3a1.41-4.8, OT-2 \u7684CV \u503c\u4e3a1.2-2.6\u3002\u719f\u7ec3\u7684\u6280\u672f \u4eba\u5458\u548cOT-2\u63d0\u4f9b\u4e86\u53ef\u6bd4\u4e14\u4e00\u81f4\u768424-26 ng\/\u03bcL\u7684\u4ea7\u91cf\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c OT-2\u80fd\u591f\u5728\u63d0\u9ad8\u4e00\u81f4\u6027\u548c\u91cd\u590d\u6027\u7684\u540c\u65f6\u63d0\u4f9b\u9ad8\u4ea7\u91cf\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"279\" height=\"249\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-123.png\" alt=\"\u663e\u793a\u81ea\u52a8\u63d0\u53d6\u7684\u4ea7\u91cf\u53d8\u5f02\u8f83\u5c0f\" class=\"wp-image-3075\"\/><figcaption class=\"wp-element-caption\"><strong>\u56fe1:\u663e\u793a\u81ea\u52a8\u63d0\u53d6\u7684\u4ea7\u91cf\u53d8\u5f02\u8f83\u5c0f\uff0c\u5e76\u4e14\u63d0\u53d6\u7eaf\u5316\u4ea7\u91cf\u53ef\u5ab2\u7f8e\u624b<\/strong>\u00a0\u00a0<strong>\u52a8\u5904\u7406\u7684\u7ed3\u679c\u3002<\/strong><\/figcaption><\/figure>\n\n\n\n<p>\u5728\u4e0e\u65b0\u51a0\u75c5\u6bd2\u76f8\u5173\u7684\u6d4b\u8bd5\u4e2d\uff0c\u4f7f\u7528\u5408\u6210\u7684 SARS-CoV-2 \u6837\u672c\u548c\u4e03\u79cd \u5e38\u7528\u7684\u63d0\u53d6\u8bd5\u5242\u76d2\u8fdb\u884c\u4e86qPCR\u3002 \u6211\u4eec\u4f7f\u7528\u4e86 SNM \u4e2d\u7684\u4eba\u7c7b\u57fa\u56e0\u7ec4 DNA \u4f5c\u4e3a\u5185\u90e8\u5bf9\u7167\uff0c\u4f7f\u7528CDC \u7684 SARS-CoV-2N1 \u548c N2 \u5f15\u7269\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\uff0c\u540c\u65f6\u4f7f\u7528 CDC \u7684 RP \u5f15\u7269\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\u3002<\/p>\n\n\n\n<p>\u8fd9\u9879\u6d4b\u8bd5\u65e8\u5728\u8bc4\u4f30\u4f7f\u7528\u4e0d\u540c\u63d0\u53d6\u8bd5\u5242\u76d2\u5728 OT-2 \u4e0a\u8fdb\u884c\u65b0\u51a0\u75c5\u6bd2 qPCR \u5206\u6790\u7684\u6027\u80fd\u3002\u901a\u8fc7\u4f7f\u7528\u5408\u6210\u7684 SARS-CoV-2 \u6837\u672c\u4ee5\u53ca\u5185\u90e8\u5bf9 \u7167\u548c\u9633\u6027\u5bf9\u7167\uff0c\u53ef\u4ee5\u5224\u65ad OT-2 \u5728\u68c0\u6d4b\u75c5\u6bd2\u57fa\u56e0\u7ec4\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\u3002<\/p>\n\n\n\n<p>\u5bf9qPCR \u6027\u80fd\u7684\u8bc4\u4f30\u662f\u57fa\u4e8e PCR \u68c0\u6d4b\u7d27\u6025\u4f7f\u7528\u6388\u6743 (EUA) \u6807\u51c6\u3002 \u4e3a\u4e86\u6ee1\u8db3 EUA \u6807\u51c6\uff0c \u4e00\u4e2a\u6d4b\u8bd5\u5fc5\u987b\u8fbe\u523095%\u7684\u6269\u589e\u6548\u679c\uff0c\u4e5f\u5c31\u662f\u8bf4\uff0c\u5728qPCR \u4e2d\uff0c95%\u7684\u6837\u672c\u5fc5\u987b\u4ea7\u751f\u53ef\u68c0\u6d4b\u7684N1\u548cN2\u4fe1\u53f7\u3002<\/p>\n\n\n\n<p>\u5728\u8fd9\u79cd\u6027\u80fd\u8bc4\u4f30\u4e2d\uff0c\u6211\u4eec\u8fd8\u5bf9 qPCR Ct \u503c\u8fdb\u884c\u4e86\u8bc4\u4f30\u3002\u5bf9\u4e8e\u8fd9\u4e9b\u76ee\u6807\uff0c\u5728\u8fd9\u7c7b\u6837\u672c\u4e2d\uff0c\u6807\u51c6 Ct\u503c\u5e94\u8be5\u572830-40\u4e4b\u95f4\u3002<\/p>\n\n\n\n<p>\u6839\u636e\u8868\u683c 3 \u7684\u7ed3\u679c\uff0cOT-2 \u51e0\u4e4e\u5728\u6240\u6709\u8bd5\u5242\u76d2\u4e0a\u7684\u8868\u73b0\u5747\u901a\u8fc7\u4e86 EUA \u6807\u51c6\u3002\u9664\u4e86\u4e24\u4e2a\u7531\u4e8e\u88c2\u89e3\u7f13\u51b2\u6db2\u8fc7\u5206\u7c98\u7a20\u5bfc\u81f4\u59a8\u788d\u4e86\u63d0\u53d6\u8fc7\u7a0b \u7684\u8bd5\u5242\u76d2\u53ea\u670991%\u7684\u6d4b\u8bd5\u7ed3\u679c\u5408\u683c\u3002\u5e76\u4e14\uff0c\u6240\u6709\u88ab\u6d4b\u8bd5\u7684\u8bd5\u5242\u4e2d\uff0c \u6837\u54c1\u7ed3\u679c\u5747\u5b9e\u73b0\u4e8631-39\u7684\u6807\u51c6 Ct \u503c\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0cOT-2 \u7cfb\u7edf\u53ef\u4ee5\u4e0e\u5404\u79cd\u54c1\u724c\u7684\u8bd5\u5242\u914d\u5957\u4f7f\u7528\uff0c\u5e76\u8fbe\u5230\u4e34\u5e8a\u6807\u51c6\u3002<\/p>\n\n\n\n<p>\u4e3a\u4e86\u6d4b\u8bd5OT-2 \u7cfb\u7edf\u7684\u541e\u5410\u91cf\u548c\u901f\u5ea6\uff0c\u6211\u4eec\u4f7f\u7528\u4e8613\u79cd\u70ed\u95e8\u8bd5\u5242\u76d2 \u8fdb\u884c\u4e86RNA \u548cDNA \u63d0\u53d6\uff0c\u5e76\u8986\u76d6\u4e86\u4e0d\u540c\u957f\u5ea6\u3002\u4e3a\u4e86\u5168\u9762\u8bc4\u4f30\u4e0d\u540c\u541e \u5410\u91cf\u4e0b\u7684\u901f\u5ea6\uff0c\u6d4b\u8bd5\u4e86\u6bcf\u62798\u300124\u300148\u548c96\u4e2a\u6837\u672c\u7684\u60c5\u51b5\u3002\u4ee5\u4ece\u539f\u59cb\u6837\u54c1\u5230\u7eaf\u5316\u6838\u9178\u6240\u9700\u7684\u65f6\u95f4\u6765\u8861\u91cf\u5b9e\u9a8c\u7ed3\u679c\u3002<\/p>\n\n\n\n<p>\u5728\u8fd9\u4e9b\u63d0\u53d6\u8bd5\u5242\u65b9\u6848\u4e2d\uff0cOT-2 \u7cfb\u7edf\u5728\u5404\u79cd\u8bd5\u5242\u76d2\u548c\u957f\u5ea6\u4e0b\u5b9e\u73b0\u4e86 \u5feb\u901f\u63d0\u53d6\u7684\u7ed3\u679c\u3002\u5728\u6700\u77ed\u7684\u534f\u8bae\u4e2d\uff0c\u5904\u74068 \u4e2a\u6837\u672c\u53ea\u9700\u898125 \u5206 \u949f\uff0c\u5728\u6700\u957f\u7684\u534f\u8bae\u4e2d\uff0c\u5904\u740696\u4e2a\u6837\u672c\u4e0d\u52305\u5c0f\u65f6(\u56fe2)\u3002\u5bf9\u4e8e8 \u4e2a\u6837\u672c\u7684\u5904\u7406\u65f6\u957f\uff0c OT-2 \u572813\u79cd\u8bd5\u5242\u76d2\u4e2d\u5e73\u5747\u5904\u7406\u65f6\u95f4\u4e3a52\u5206 \u949f\u3002\u800c\u572896\u4e2a\u6837\u672c\u7684\u60c5\u51b5\u4e0b\uff0c OT-2 \u572813\u79cd\u8bd5\u5242\u76d2\u4e2d\u5e73\u5747\u5904\u7406\u65f6\u95f4 \u4e3a2\u5c0f\u65f645\u5206\u949f\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c OT-2\u7cfb\u7edf\u53ef\u4ee5\u5c06\u5176\u9ad8\u6027\u80fd\u4e0e\u9ad8\u541e\u5410\u91cf\u76f8\u7ed3\u5408\uff0c\u663e\u8457\u6539\u5584\u63d0\u53d6\u5de5\u4f5c\u6d41\u7a0b\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"505\" height=\"229\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-124.png\" alt=\"\u4e0d\u540c\u63d0\u53d6\u8bd5\u5242\u76d2\u6570\u636e\" class=\"wp-image-3076\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-124.png 505w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-124-300x136.png 300w\" sizes=\"(max-width: 505px) 100vw, 505px\" \/><figcaption class=\"wp-element-caption\">\u88682:\u8fd9\u4e2a\u8868\u683c\u5c55\u793a\u4e86\u4f7f\u7528\u624b\u52a8\u548c\u81ea\u52a8\u5904\u7406 \u65b9\u6cd5\u8fdb\u884c\u7684\u4e0d\u540c\u63d0\u53d6\u8bd5\u5242\u76d2\u5728\u6536\u7387\u548c\u7cbe\u786e \u6027\u65b9\u9762\u7684\u53ef\u6bd4\u6027\u3002\u8868\u683c\u5de6\u4fa7\u663e\u793a\u4e86\u5e73\u5747\u53d8 \u5f02\u7cfb\u6570 (CV) \u548c\u5faa\u73af\u9608\u503c (Ct)\u3002<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"681\" height=\"187\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-125.png\" alt=\"\u5c55\u793a\u4e86OT-2 \u81ea\u52a8\u5316\u7684\u5e7f\u6cdb\u9002\u7528\u6027\" class=\"wp-image-3077\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-125.png 681w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-125-300x82.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-125-576x158.png 576w\" sizes=\"(max-width: 681px) 100vw, 681px\" \/><figcaption class=\"wp-element-caption\">\u88683:\u8fd9\u4e2a\u8868\u683c\u8bf4\u660e\u4e86\u4f7f\u7528SARS-CoV-2 \u75c5\u6bd2\u6d4b\u8bd5\u7684\u8d28\u63a7\u89c4\u8303\uff0c\u5c55\u793a\u4e86OT-2 \u81ea\u52a8\u5316\u7684\u5e7f\u6cdb\u9002\u7528\u6027\u3002\u4f7f\u75287\u4e2a\u4e0d\u540c\u54c1\u724c\u76847\u79cd\u75c5\u6bd2\u8bd5\u5242\u76d2\u5728OT-2 \u4e0a\u8fdb\u884c\u81ea\u52a8\u5316\u5904\u7406\u540e\uff0c\u6bcf\u79cd\u8bd5\u5242\u76d2 (n=22) 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\u81ea\u52a8\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad9\u7684\u7532\u677f\u5e03\u5c40\u548c\u8fd0\u884c\u65f6\u95f4\uff0c\u9002\u7528\u4e8e\u6700\u591a96\u4e2a\u6837\u54c1\u3002<br>(A)\u5bf9\u5e9496\u4e2a\u6837\u54c1\u901a\u91cf\u7684RNA\u548cDNA\u63d0\u53d6\u7684OT-2\u7532\u677f\u5e03\u5c40\uff0c\u4f7f\u7528Opentrons \u63d0\u4f9b\u7684\u5b9e\u9a8c\u5ba4\u8017\u6750\u3002<br>(B)RNA \u548cDNA \u63d0\u53d6\u534f\u8bae\u4e2d8\u4e2a\u300124\u4e2a\u300148\u4e2a\u548c96\u4e2a\u6837\u54c1\u7684\u8fd0\u884c\u65f6\u95f4\u3002<\/figcaption><\/figure>\n\n\n\n<p>\u7814\u7a76\u601d\u8003<br>OT-2 \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\u4ece\u6709\u673a\u548c\u5408\u6210\u539f\u59cb\u6a21\u677f\u4e2d\u63d0\u4f9b\u4e86\u4f18\u8d28\u7684 RNA \u548c DNA \u6837\u54c1\u3002\u901a\u8fc7 qPCR \u548c\u8367\u5149\u6d4b\u8bd5\u53d1\u73b0\uff0c\u8be5\u81ea\u52a8\u5316\u7cfb\u7edf\u4ea7\u751f\u7684 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