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\u5b58\u5728\u6838\u8863\u58f3(N)\u86cb\u767d\u548c\u523a\u7a81(S)\u86cb\u767d\u6cbb\u7597\u4e25\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7edf\u7efc\u5408\u5f81\u51a0\u72b6\u75c5\u6bd22\uff08SARS-CoV-2\uff09\u3002\u6297\u4f53\u68c0\u6d4b\u53ef\u7528\u4e8e\u68c0\u6d4b\u7531\u4e8e\u75c5\u6bd2\u611f\u67d3\u800c\u5bfc\u81f4\u7684\u8840\u6e05\u4e2d\u8fd9\u4e9b\u4e2d\u548c\u6297\u4f53\u7684\u5b58\u5728\uff0c\u63d0\u4f9b\u89e3\u51b3\u4eba\u7fa4\u6216\u8fc7\u53bbSARS-CoV-2\u611f\u67d3\u7684\u4fe1\u606f\uff0c\u4ee5\u66f4\u597d\u5730\u4e86\u89e3SARS-CoV-2\u7684\u6d41\u884c\u75c5\u5b66\u3002\u8fd9\u4e9b\u6d4b\u8bd5\u662f\u5728\u4e34\u5e8a\u8bd5\u9a8c\u4e2d\u786e\u5b9a\u75ab\u82d7\u6548\u529b\u7684\u5e38\u7528\u5de5\u5177\u4ee5\u53ca\u5728\u5927\u89c4\u6a21\u63a5\u79cd\u75ab\u82d7\u4e4b\u540e\u3002
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\u6216\u8005\uff0c\u78c1\u73e0\u4e5f\u53ef\u4ee5\u4f5c\u4e3aelisa\u4e2d\u7684\u56fa\u4f53\u8868\u9762\u3002\u5176\u4f18\u70b9\u5305\u62ec\u4e00\u4e2a\u589e\u52a0\u7684\u5728\u6574\u4e2a\u6837\u54c1\u5904\u7406\u8fc7\u7a0b\u4e2d\uff0c\u53ef\u7528\u7684\u8868\u9762\u79ef\u548c\u73e0\u5b50\u7684\u5747\u5300\u5206\u5e03\uff0c\u53ef\u4ee5\u5b9e\u73b0\u66f4\u5feb\u901f\u548c\u7075\u654f\u7684\u68c0\u6d4b\u4f4e\u5206\u6790\u7269\u6d53\u5ea6\u3002\u6211\u4eec\u5f00\u53d1\u4e86\u4e00\u79cd\u57fa\u4e8e\u73e0\u5b50\u7684ELISA\uff0c\u7528\u4e8e\u68c0\u6d4bSARS-CoV-2\u75c5\u6bd2\u7684\u4e2d\u548c\u6297\u4f53\u3002\u8be5\u68c0\u6d4b\u65b9\u6cd5\u5229\u7528\u4e86SARS-CoV-2\u7684\u53d7\u4f53\u7ed3\u5408\u57df\uff08RBD\uff09S\u86cb\u767d\u4f5c\u4e3a\u6355\u83b7\u6297\u539f\uff0c\u8d5b\u9ed8\u8d39\u96eaherDynabeads\uff08\u6c83\u5c14\u745f\u59c6\uff0cMA\uff0cUSA\uff09\u4f5c\u4e3a\u56fa\u4f53\u8868\u9762\u3002
\u5c3d\u7ba1\u68c0\u6d4b\u8bbe\u8ba1\u591a\u6837\uff0celisa\u901a\u5e38\u9075\u5faa\u7c7b\u4f3c\u7684\u5de5\u4f5c\u6d41\u3002\u4e00\u822c\u6b65\u9aa4\u5305\u62ec\u8bd5\u5242\u8f6c\u79fb\u3001\u5b75\u80b2\u548c\u91cd\u590d\u6d17\u6da4\u3002\u57fa\u4e8e\u73e0\u7684elisa\u7a0b\u5e8f\u9700\u8981\u5355\u72ec\u7684\u4eea\u5668\u6765\u6267\u884c\u8fd9\u4e9b\u6b65\u9aa4\uff0c\u8fd9\u53ef\u80fd\u5728Opentrons OT-2\u5e73\u53f0\u4e0a\u81ea\u52a8\u5316\u78c1\u6027\u6a21\u5757\u548c\u52a0\u70ed\u5668\u632f\u52a8\u5668\u3002\u6211\u4eec\u5f00\u53d1\u4e86\u4e00\u4e2a\u7b80\u5316\u68c0\u6d4b\u65b9\u6848\uff0c\u4ee5\u907f\u514d\u591a\u79cd\u4eea\u5668\u7684\u9700\u8981\uff0c\u5e76\u51cf\u5c11\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u3002\u8be5\u65b9\u6848\u8fd8\u4f7f\u7528ACROBio\u7cfb\u7edf\u516c\u53f8\u7684RBD\u5076\u8f6d\u78c1\u73e0\uff08Newark\uff0cDE\uff0cUSA\uff09\u8fdb\u884c\u9a8c\u8bc1\uff0c\u4ee5\u786e\u8ba4OT-2\u6837\u54c1\u5904\u7406\u7684\u8d28\u91cf\u3002<\/p>\n\n\n\n

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\u5c0660 \u03bcg SARS-CoV-S-2S\u86cb\u767dhis\u6807\u8bb0RBD\u7ed3\u5408\u5236\u5907RBD\u5076\u8054\u73e0\uff08acro\u751f\u7269\u7cfb\u7edf\u516c\u53f8\uff0c\u7ebd\u74e6\u514b\uff0cDE\uff0c\u7f8e\u56fd\uff09\u81f35\u6beb\u514b\u8fea\u7eb3\u6bd4\u5fb7\uff08\u8d5b\u9ed8\u8d39\u820d\u5c14\uff0c\u6c83\u5c14\u745f\u59c6\uff0c\u9a6c\u8428\u8bf8\u585e\u5dde\uff0c\u7f8e\u56fd\uff09\uff0c\u8d85\u987a\u78c1\u6027\u7403\u5f62\u805a\u5408\u7269\u7c92\u5b50\u4e3a\u78c1\u5206\u79bb\u751f\u7269\u6750\u6599\uff0c\u7136\u540e\u50a8\u5b58\u57282.5 mL\u7684\u5c01\u95ed\u7f13\u51b2\u6db2\u4e2d\u3002\u76f8\u540c\u7684\u534f\u8bae\u4e5f\u662f\u901a\u8fc7\u4f7f\u7528\u5e02\u552e\u7684RBD\u8fdb\u884c\u6d4b\u8bd5\u7684\u5417\u7f8e\u56fd\u751f\u7269\u7cfb\u7edf\u7684\u9884\u6d82\u5c42\u73e0(36 \u03bcg RBD\u6bcf\u6beb\u514b\u73e0\u5b50\uff09\u3002\u4eba\u6297sars-cov-2S1\u6297\u4f53\u4f5c\u4e3a\u6807\u51c6\u6297\u4f53\u548c\u5c0f\u9f20\u5355\u514b\u9686\u6297\u4eba\u6297\u4f53\u4ee5HRP\u5076\u8054\u4f5c\u4e3a\u68c0\u6d4b\u6297\u4f53\u7684IgG Fc\u8d2d\u81ea\u7ec6\u80de\u79d1\u5b66\u516c\u53f8\uff08\u7ebd\u4f2f\u91cc\u6ce2\u7279\uff0cMA\uff0c\u7f8e\u56fd\uff09\u548cAbcam\u516c\u53f8\uff08\u5251\u6865\uff0c\u82f1\u56fd\uff09\uff0c\u5b9a\u5236\u7684\u68c0\u6d4b\u7f13\u51b2\u6db2\u5206\u522b\u7531. \u7279\u8bfa\u74e6\uff08\u970d\u5229\u65af\u7279\uff0c\u52a0\u5229\u798f\u5c3c\u4e9a\u5dde\uff0c\u7f8e\u56fd\uff09\u3002
\u6db2\u4f53\u8f6c\u79fb\u75318\u901a\u9053\u79fb\u6db2\u7ba1\u8fdb\u884c\u3002ELISA\u677f\u88ab\u653e\u7f6e\u5728\u78c1\u6a21\u5757\u4e0a\uff0c\u5e76\u624b\u52a8\u79fb\u52a8\u5230\u52a0\u70ed\u5668\u6a21\u5757\u5982\u679c\u9700\u8981\u6405\u62cc\u3002\u672c\u7814\u7a76\u83b7\u5f97\u4e86\u4ee5\u524d\u68c0\u6d4b\u4e3aSARS-CoV-2\u6297\u4f53\u9633\u6027\u6216\u9634\u6027\u7684\u8840\u6e05\u6837\u672c\u3002\u5b9e\u9a8c\u5b8c\u6210\u540e\uff0c\u7acb\u5373\u7528\u6bd4\u8272\u5e73\u677f\u4eea\u5728450 nm\u6ce2\u957f\u4e0b\u6d4b\u5b9a\u6bcf\u4e2a\u6837\u54c1\u7684\u5438\u5149\u5ea6\u3002\u7684\u8fd0\u884c\u65f6\u5bf9\u4e0d\u540c\u6837\u672c\u91cf\u4e0b\u7684\u6bcf\u79cd\u68c0\u6d4b\u65b9\u6cd5\u8fdb\u884c\u4e86\u4f30\u8ba1\uff08\u88681\uff09\u3002<\/p>\n\n\n\n

\"\u7814\u7a76\u4e2d\u6d4b\u8bd5\u7684\u57fa\u4e8e\u73e0\u5b50\u7684ELISAs\u7684\u673a\u5236\"<\/figure>\n\n\n
\n
\"\"<\/figure><\/div>\n\n\n

\u7ed3\u679c
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\u6765\u81ea\u5b8f\u5b8f\u751f\u7269\u7cfb\u7edf\u7684RBD\u9884\u6d82\u5c42\u73e0\u7136\u540e\u91cd\u7ec4\uff08\u6bcfmL\u963b\u65ad\u7f13\u51b2\u6db21mg\u73e0\u5b50\uff09\uff0c\u5e76\u5728OT-2\u4e0a\u4f7f\u7528\u76f8\u540c\u7684\u65b9\u6848\u8fdb\u884c\u6d4b\u8bd5\u3002\u5236\u5907\u8fde\u7eed2\u500d\u7a00\u91ca\u7684\u4eba\u6297sars-cov-2S1\u6297\u4f53\uff0c\u5e76\u66b4\u9732\u4e8e20 \u03bcL\u9762\u5305\u60ac\u6db2\u4e2d\u3002\u8fd9\u4e9b\u6570\u636e\u88ab\u7528\u4e8e\u7ed8\u5236\u5242\u91cf-\u53cd\u5e94\u66f2\u7ebf\uff0c\u4ee5\u4f9b\u8fdb\u4e00\u6b65\u5206\u6790\u3002OT-2\u4e0a\u6837\u54c1\u5904\u7406\u7684\u4e00\u81f4\u6027\uff08CV<10%\uff09\uff08\u56fe3A\uff09\u548c\u68c0\u6d4b\u8bfb\u6570\u4e0e\u6297\u4f53\u6d53\u5ea6\u7684\u7ebf\u6027\u5173\u7cfb\u5747\u5f97\u5230\u4e86\u8bc1\u5b9e\uff08100 ng\/mL\u81f36.25 ng\/mL\uff0cr\u5e73\u65b9>0.99\uff09\uff08\u56fe3B\uff09\u3002<\/p>\n\n\n\n

\u4e3a\u4e86\u8fdb\u884c\u57fa\u4e8e\u73e0\u5b50\u7684ELISA\uff0c\u4ee5\u786e\u5b9aSARS-CoV-2 S1\u4e2d\u548c\u6297\u4f53\u7684\u6c34\u5e73\uff0c\u8be5\u65b9\u6848\u8bbe\u8ba1\u7528\u4e8eRBD\u5076\u8054\u73e0\u5b50\u7684\u5236\u5907\u623f\u5b50\u6216\u7f8e\u56fd\u751f\u7269\u7cfb\u7edf\u516c\u53f8\u7684RBD\u5171\u8f6d\u9762\u5305\u662f\u7528\u4e8e\u5904\u7406\u4e4b\u524d\u7531\u4f20\u7edf\u7684\u3001fda\u8ba4\u8bc1\u7684ELISA\u68c0\u6d4b\u7684\u8840\u6e05\u6837\u672c\uff08\u9633\u6027\uff1a *\uff09\u3002\u7ed3\u679c\u518d\u6b21\u663e\u793a\u4e86OT-2\u6db2\u4f53\u5904\u7406\u7684\u7cbe\u5ea6\uff08CV <\u4e3a10%\uff09\u548c\u6297\u4f53\u6d53\u5ea6\u6839\u636e\u56fe4\u6240\u793a\u7684\u6807\u51c6\u66f2\u7ebf\u5f97\u5230\u3002<\/p>\n\n\n\n

\u5bf9OT-2\u6297\u4f53\u7684\u6297\u4f53\u4e2d\u548c\u8bd5\u9a8c<\/p>\n\n\n\n

\"\u5185\u90e8\u5f00\u53d1\u7684SARS-CoV-2\u75c5\u6bd2\u4e2d\u548c\u8bd5\u9a8c\"
\u56fe2\uff1a\u5185\u90e8\u5f00\u53d1\u7684SARS-CoV-2\u75c5\u6bd2\u4e2d\u548c\u8bd5\u9a8c\u5728OT-2\u4e0a\u6210\u529f\u8fdb\u884c\uff0c\u6837\u54c1\u5904\u7406\u9ad8\u5ea6\u4e00\u81f4\uff08A\uff0cCV\uff0c\u6bcf\u4e2a\u6d53\u5ea6\u91cd\u590d\u5904\u7406\u4e09\u6b21\uff09\uff0c\u5242\u91cf\u54cd\u5e94\u66f2\u7ebf\u5728100 ng\/mL~12.5 ng\/mL (B)\u4e4b\u95f4\u7ebf\u6027\u5f97\u5230<\/figcaption><\/figure>\n\n\n\n

\u5728OT-2\u4e0a\u7684\u57fa\u4e8e\u73e0\u5b50\u7684ELISA<\/p>\n\n\n\n

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\u56fe3\uff1a\u6765\u81eaACROBio\u7cfb\u7edf\u516c\u53f8\u7684RBD\u9884\u6d82\u8986\u73e0\u5b50\u5728OT-2\u4e0a\u6d4b\u8bd5\u4e86\u57fa\u4e8e\u73e0\u5b50\u7684ELISA\u534f\u8bae\u3002\u786e\u8ba4\u4e86\u6837\u54c1\u5904\u7406\u7684\u8d28\u91cf\uff08A\uff0c\u901a\u8fc7\u4e09\u6b21\u91cd\u590d\u5904\u7406\u6bcf\u4e2a\u6d53\u5ea6\u83b7\u5f97\u7684CV\uff09\u4ee5\u53ca\u68c0\u6d4b\u8bfb\u6570\u4e0e\u6297\u4f53\u6d53\u5ea6\uff08100 ng\/mL\u81f36.25 ng\/mL\uff0cB\uff09\u4e4b\u95f4\u7684\u7ebf\u6027\u5173\u7cfb\u3002<\/figcaption><\/figure>\n\n\n\n
\"\u4f7f\u7528\u5185\u90e8(A)\u5236\u5907\u7684RBD\u5076\u8054\u73e0\u7684\u73e0\u7c92\u68c0\u6d4b\"
\u56fe4\uff1a\u4f7f\u7528\u5185\u90e8(A)\u5236\u5907\u7684RBD\u5076\u8054\u73e0\u7684\u73e0\u7c92\u68c0\u6d4b\uff0c\u4ee5\u53caACROBio\u7cfb\u7edfRBD\u5076\u8054\u73e0(B)\u5bf9\u4eba\u8840\u6e05\u6837\u672c\u4e2d\u5b58\u5728\u7684OT-2\u6d4b\u91cf\u7684\u4e2d\u548c\u6297\u4f53\u8fdb\u884c\u68c0\u6d4b\uff0c\u7cbe\u5ea6\u6781\u597d\uff08CV < 10%\uff09\u3002\u6297\u4f53\uff0c\u6297\u4f53\u6d53\u5ea6\u4e5f\u88ab\u6d4b\u5b9a\u4e86\u3002\u4e4b\u524d\u7ecffda\u8ba4\u8bc1\u7684ELISA\uff08*\uff09\u68c0\u6d4b\u4e3a\u9633\u6027\u7684\u6837\u672c\u901a\u8fc7\u8be5\u68c0\u6d4b\u5f97\u5230\u786e\u8ba4\u3002<\/figcaption><\/figure>\n\n\n\n

\u7ed3\u8bba
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