{"id":3008,"date":"2024-06-25T23:15:10","date_gmt":"2024-06-25T15:15:10","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=3008"},"modified":"2024-09-02T18:57:14","modified_gmt":"2024-09-02T10:57:14","slug":"opentrons-pcr-gzzzdhpcr","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/opentrons-pcr-gzzzdhpcr\/","title":{"rendered":"Opentrons PCR \u5de5\u4f5c\u7ad9\u81ea\u52a8\u5316PCR\u5236\u5907\u53cd\u5e94\u65b9\u6848"},"content":{"rendered":"\n<p><strong>\u6458\u8981<\/strong><br>\u968f\u7740\u751f\u547d\u79d1\u5b66\u5c4a\u5bf9PCR\u7684\u9700\u6c42\u6b63\u5728\u589e\u52a0\u3002\u5728\u672c\u5e94\u7528\u8bf4\u660e\u4e2d\uff0c\u6211\u4eec\u5c55\u793a\u4e86 Opentrons PCR \u5de5\u4f5c\u7ad9\u4e0e\u5e02\u9762\u4e3b\u6d41\u7684\u5916\u7f6e\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u76f8 \u6bd4\uff0c\u80fd\u591f\u751f\u6210\u9884\u671f\u7684PCR\u6269\u589e\u4ea7\u7269\u7684\u78b1\u57fa\u5bf9\u957f\u5ea6\u3002\u7ed3\u679c\u663e\u793a\uff0c\u65e0\u8bba\u662f\u5d4c\u5957PCR\u8fd8\u662f\u591a\u91cdPCR, \u90fd\u80fd\u4ea7\u751f\u9884\u671f\u7684 PCR\u6269\u589e\u4ea7\u7269\u7684\u78b1\u57fa \u5bf9\u957f\u5ea6\u3002<\/p>\n\n\n\n<p><strong>\u7b80\u4ecb<\/strong><br>\u7531\u4e8e\u6837\u54c1\u5236\u5907\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u5b58\u5728\u6c61\u67d3\u3001\u6280\u672f\u9519\u8bef\u548c\u65f6\u95f4\u9650\u5236\uff0c\u590d\u6742\u65b9\u6848(\u4f8b\u5982\u5de2\u5f0f PCR \u548c\u591a\u91cd PCR) \u7684\u6548\u7387\u548c\u51c6\u786e\u6027\u96be\u4ee5\u4fdd\u969c\u3002\u591a\u91cd PCR \u5b9e\u9a8c\u7528\u4e8e GMO (\u8f6c\u57fa\u56e0\u751f\u7269)\u68c0\u6d4b\u3001\u6cd5\u533b\u5b66\u3001\u98df\u54c1\u5206\u6790\u3001\u7a81\u53d8\u548c\u591a\u6001\u6027\u68c0\u6d4b\u3001\u57fa\u56e0\u7f3a\u5931\u5206\u6790\u3001\u6a21\u677f\u5b9a\u91cf\u3001\u8fde\u9501\u5206\u6790\u4ee5\u53ca\u66f4\u591a\u5e94 \u7528\u3002\u8fd9\u4e9b\u591a\u91cd\u53cd\u5e94\u975e\u5e38\u9ad8\u6548\uff0c\u53ef\u5728\u5b54\u69fd\u4e2d\u4ea7\u751f\u4e24\u4e2a\u6216\u591a\u4e2a\u6269\u589e\u5b50\u7684\u4ea7\u7269\u3002<\/p>\n\n\n\n<p>\u5de2\u5f0f PCR \u662f\u53e6\u4e00\u79cd\u9ad8\u5ea6\u7279\u5f02\u6027\u7684\u6280\u672f\uff0c\u53ef\u7528\u4f5c\u6709\u7528\u7684\u8bca\u65ad\u5de5\u5177\uff0c\u7528\u4e8e\u8bc6\u522b\u751f\u7269\u6837\u54c1\u4e2d\u7684\u75c5\u539f\u4f53\uff0c\u4f8b\u5982\u52a8\u8109\u7ca5\u6837\u786c\u5316\u6591\u5757\u4e2d\u7684\u7259\u5468\u75c5 \u539f\u4f53\u3001\u8f6c\u79fb\u6027\u4e73\u817a\u764c\u7ec6\u80de\u4ee5\u53ca\u7ed3\u6838\u5206\u679d\u6746\u83cc\u548c\u9a6c\u5c14\u5c3c\u83f2\u9752\u9709\u7b49\u75c5\u6bd2\u75c5\u539f\u4f53\u4e0e\u4ec5\u9700\u8981\u4e00\u4e2a\u6269\u589e\u6b65\u9aa4\u7684\u6807\u51c6PCR \u4e0d\u540c\uff0c\u5de2\u5f0f PCR \u5177\u6709\u4e24 \u6b65\u6269\u589e\u8fc7\u7a0b\u3002\u7b2c\u4e00\u4e2a PCR \u6269\u589e\u6b65\u9aa4\u4e2d\u751f\u6210\u7684 PCR \u6269\u589e\u5b50\u7528\u4f5c\u7b2c\u4e8c\u4e2a\u6269\u589e\u6b65\u9aa4\u7684\u6a21\u677f\u3002\u8fd9\u4e9b\u590d\u5408 PCR \u5b9e\u9a8c\u53ef\u80fd\u4f1a\u56e0\u6700\u7ec8\u4ea7\u54c1\u7684\u4e0d\u540c \u800c\u53d8\u5f97\u7e41\u7410\u548c\u8017\u65f6\u3002Opentrons PCR \u5de5\u4f5c\u7ad9\u53ef\u4ee5\u81ea\u52a8\u5316\u5b9e\u73b0 PCR \u5b9e\u9a8c\uff0c\u53ea\u9700\u8981\u8fdb\u884c\u975e\u5e38\u5c11\u91cf\u7684\u624b\u52a8\u51c6\u5907\u5de5\u4f5c\uff0c\u5c31\u53ef\u4ee5\u4ea7\u51fa\u7a33\u5b9a\u7684\u4e00 \u81f4\u6027\u7ed3\u679c\u3002<\/p>\n\n\n\n<p>\u6750\u6599\u548c\u65b9\u6cd5<\/p>\n\n\n\n<p><strong>\u591a\u91cd PCR \u5b9e\u9a8c\u6b65\u9aa4<\/strong><br>\u4f7f\u7528\u4ece\u94dc\u7eff\u5047\u5355\u80de\u83cc\u548c\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u5206\u79bb\u7684\u57fa\u56e0\u7ec4 DNA \u8fdb\u884c\u4e86\u591a\u91cd PCR \u5b9e\u9a8c\u3002\u6839\u636e\u5148\u524d\u7684\u7814\u7a76\uff0c\u8bbe\u8ba1\u4e86\u9488\u5bf9\u94dc\u7eff\u5047\u5355\u80de\u83cc (lasl\u3001lasR\u3001gyrB) \u548c\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc(16s rRNA\u3001clfA\u3001mecA\u3001Eubacterial 16S rRNA)\u591a\u4e2a\u4f4d\u70b9\u533a\u57df\u7279\u5f02\u6027\u7684\u6b63\u5411\u548c\u53cd\u5411<br>\u5f15\u7269\u3002\u6bcf\u4e2a\u5b54\u9884\u671f\u4ea7\u7269\u7684\u5927\u5c0f\u5206\u522b\u4e3a600\u3001700\u548c222bp\u3002 \u5728 Opentrons OT-2 \u7684\u5e73\u53f0\u4e0a\uff0c\u57280.1 ml 96 \u5b54\u5168\u88d9\u8fb9 NEST \u677f\u4e0a\u51c6\u5907 \u4e8620 \u03bcL\u7684\u591a\u91cd PCR\u53cd\u5e94\u6df7\u5408\u7269\uff0c\u5e76\u4f7f\u7528\u8bbe\u5907\u4e0a\u7684\u70ed\u5faa\u73af\u4eea\u8fdb\u884c\u6269\u589e\u3002\u6269\u589e\u4ea7\u7269\u7ecf2%\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5206\u6790\u3002\u540c\u65f6\uff0c\u4e3a\u4e86\u5bf9\u6bd4\u5206\u6790\uff0c \u6211\u4eec\u4e5f\u7528\u624b\u52a8\u51c6\u5907\u4e86\u76f8\u540c\u7684\u5b9e\u9a8c\uff0c\u5e76\u5728\u7b2c\u4e09\u65b9\u624b\u52a8\u70ed\u5faa\u73af\u4eea\u4e0a\u8fd0\u884c\u3002<\/p>\n\n\n\n<p><strong>\u5de2\u5f0f PCR \u5b9e\u9a8c\u6b65\u9aa4<\/strong><br>\u5de2\u5f0f PCR \u662f\u7528\u4e8e\u9274\u5b9a\u4ece lambda \u566c\u83cc\u4f53\u5206\u79bb\u7684\u751f\u7269\u6837\u54c1\u4e2d\u7684\u75c5\u539f\u4f53\u7684\u65b9\u6cd5\u3002\u6839\u636e\u5148\u524d\u7684\u7814\u7a76\uff0c\u9488\u5bf9\u94dc\u7eff\u5047\u5355\u80de\u83cc (lasl\u3001lasR\u3001 gyrB) \u548c\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc(16S rRNA\u3001clfA\u3001mecA\u3001Eubacterial 16S rRNA) \u8bbe\u8ba1\u4e86\u7279\u5f02\u6027\u7684\u591a\u4e2a\u4f4d\u70b9\u533a\u57df\u7684\u6b63\u5411\u548c\u53cd\u5411\u5f15\u7269\u3002 PCR\u7ec4\u5206\u88ab\u63d0\u4f9b\u7ed9OT-2 \u5e73\u53f0\uff0c\u5e76\u8f6c\u79fb\u52300.1 ml96 \u5b54 NEST\u677f\u4e2d\u3002\u5728\u6df7\u5408\u548c Opentrons \u70ed\u5faa\u73af\u6a21\u5757\u81ea\u52a8\u5bc6\u5c01\u4e4b\u524d\uff0c\u6700\u540e\u52a0\u5165 DNA \u6a21\u677f\u3002\u5bf9\u4e8e\u7b2c\u4e8c\u4e2a\u6269\u589e\u6b65\u9aa4\uff0c\u4f7f\u75280.1 ml \u7684 NEST \u5b54\u677f\u91cd\u590d\u6b64\u8fc7\u7a0b\uff0c\u5e76\u5c06\u5176\u5bc6\u5c01\u540e\u653e\u7f6e\u5728\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u3002\u7b2c\u4e00\u6b21\u6269\u589e\u7684\u6269\u589e\u4ea7 \u7269\u88ab\u7528\u4f5c\u9633\u6027\u5bf9\u7167\u3002<\/p>\n\n\n\n<p>PCR\u4ea7\u7269\u7ecf2%\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5206\u6790\u3002\u6240\u6709\u5b9e\u9a8c\u90fd\u4f7f\u7528Qubit BR\u5b9a\u91cf\uff0c\u5e76\u8ba1\u7b97\u53d8\u5f02\u7cfb\u6570\u4ee5\u8bc4\u4f30\u6269\u589e\u7684\u4e00\u81f4\u6027\u3002<\/p>\n\n\n\n<p><strong>\u5b9e\u9a8c\u7ed3\u679c<\/strong><br>\u4f7f\u7528Opentrons \u70ed\u5faa\u73af\u6a21\u5757\u5728OT-2\u4e0a\u8fdb\u884c\u81ea\u52a8\u5316\u591a\u91cdPCR\u5b9e\u9a8c\uff0c\u8fd0\u884c\u7ed3\u679c\u663e\u4e0e\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u76f8\u5f53\u3002<\/p>\n\n\n\n<p>\u591a\u91cd PCR\u7684\u5b9e\u9a8c\u5e03\u5c40\u91c7\u7528\u4e86 Kim \u7b49\u4eba\u57282018\u5e74\u7684\u7814\u7a76\u4e2d\u7684\u65b9\u6cd5\u3002\u6837\u54c1\u5b54\u4f4d\u6807\u8bb0\u4e3a\u7eff\u8272\uff0c\u9634\u6027\u5bf9\u7167\u6807\u8bb0\u4e3a\u84dd\u8272(\u89c1\u56fe1)\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"640\" height=\"210\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-71.png\" alt=\"Opentrons PCR \u5de5\u4f5c\u7ad9\u81ea\u52a8\u5316\" class=\"wp-image-3009\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-71.png 640w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-71-300x98.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-71-576x189.png 576w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n\n\n\n<p>\u4e3a\u4e86\u8bc4\u4f30\u81ea\u52a8\u5316\u591a\u91cd PCR \u7684\u9ad8\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\uff0c\u5728\u51dd\u80f6\u4e0a\u8fdb\u884cPCR \u4ea7\u7269\u68c0\u6d4b\u3002\u4e0e\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u751f\u6210\u7684PCR \u4ea7\u7269\u76f8\u6bd4(\u56fe2B), OT-2\u70ed\u5faa\u73af\u4eea\u4e0a\u751f\u6210\u7684 PCR \u6269\u589e\u4ea7\u7269\u4e0d\u4ec5\u663e\u793a\u51fa\u66f4\u6e05\u6670\u548c\u660e\u663e\u7684\u6761\u5e26\uff0c\u800c\u4e14\u957f\u5ea6\u7b26\u5408\u9884\u671f (lasl\u4e3a600bp,lasR \u4e3a700bp,gyrB \u4e3a222bp) ( \u56fe 2A)\u3002<br>\u4e3a\u4e86\u8fdb\u4e00\u6b65\u8bc1\u660e OT-2 \u7684\u6548\u7387\uff0c\u4f7f\u7528\u9488\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc(16s rRNA \u3001clfA \u3001mecA \u3001Eubacterial 16s rRNA) \u7684\u7279\u5f02\u6027\u5f15\u7269\u8fdb\u884c \u4e86\u81ea\u52a8\u5316\u591a\u91cd PCR\u3002\u9884\u671f\u7684\u78b1\u57fa\u5bf9\u957f\u5ea6\u5206\u522b\u4e3a791bp\u3001638bp\u3001499bp \u548c371bp, \u5e76\u5728\u743c\u8102\u7cd6\u51dd\u80f6\u4e0a\u68c0\u6d4b(\u56fe2C) \u3002 \u540c\u65f6\uff0c\u4f7f \u7528\u76f8\u540c\u7684PCR\u7ec4\u5206\u8fdb\u884c\u4e86\u624b\u52a8\u591a\u91cd PCR\u534f\u8bae\uff0c\u4f7f\u7528\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u8fdb\u884c\u64cd\u4f5c(\u56fe2)\u3002\u5728 OT-2\u4e0a\u81ea\u52a8\u5316\u548c\u624b\u52a8\u8fdb\u884c\u7684\u591a\u91cd PCR\u90fd \u663e\u793a\u51fa\u4e86\u7c7b\u4f3c\u7684\u6269\u589e\u6548\u679c(\u56fe2A\u30012B)\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"807\" height=\"386\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-72.png\" alt=\"\u5728OT-2\u4e0a\u7684\u5b9e\u9a8c\u6570\u636e\" class=\"wp-image-3010\" style=\"width:595px;height:auto\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-72.png 807w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-72-300x143.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-72-768x367.png 768w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-72-576x276.png 576w\" sizes=\"(max-width: 807px) 100vw, 807px\" \/><figcaption class=\"wp-element-caption\">\u56fe2:\u5728OT-2\u548c\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u5206\u522b\u8fdb\u884c\u94dc\u7eff\u5047\u5355\u80de\u83cc\u548c\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u76ee\u6807\u57fa\u56e0\u7684\u591a\u91cd\u00a0PCR<\/figcaption><\/figure>\n\n\n\n<p>\u5728\u6bcf\u4e2a\u5b54\u4f4d\u4e0a\u6d4b\u8bd5\u7684\u94dc\u7eff\u5047\u5355\u80de\u83cc\u57fa\u56e0\u7ec4DNA \u4e2d\u6269\u589e\u4e86\u9884\u671f\u957f\u5ea6\u4e3a600\u3001700\u548c222 bp \u7684lasl\u3001lasR\u548cgyrB\u76ee\u6807\u3002<br>A)1-8 \u9053\u663e\u793a\u4e86\u5728OT-2 \u4e0a\u81ea\u52a8\u52a0\u6837\u548c\u70ed\u5faa\u73af\u540e\u7684\u4ea7\u7269\u3002<br>B)1-8 \u9053\u663e\u793a\u4e86\u5728\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u624b\u52a8\u52a0\u6837\u540e\u7684\u4ea7\u7269\u3002\u5728\u6bcf\u4e2a\u5b54\u4f4d\u4e0a\u6d4b\u8bd5\u7684\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u57fa\u56e0\u7ec4DNA \u4e2d\u6269\u589e\u4e86\u9884\u671f\u957f\u5ea6\u4e3a 791\u3001638\u3001499\u548c371 bp \u768416S rRNA\u3001clfA\u3001mecA\u548c\u771f\u83cc\u768416S rRNA\u76ee\u6807\u3002<br>C)1-8 \u9053\u663e\u793a\u4e86\u5728OT-2 \u4e0a\u81ea\u52a8\u52a0\u6837\u548c\u70ed\u5faa\u73af\u540e\u7684\u4ea7\u7269\u3002<br>D)1-8 \u9053\u663e\u793a\u4e86\u5728\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u624b\u52a8\u52a0\u6837\u540e\u7684\u4ea7\u7269\u3002<\/p>\n\n\n\n<p>\u4e3a\u4e86\u9a8c\u8bc1\u5728OT-2 \u548c\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u751f\u6210\u7684 PCR \u7684\u7cbe\u786e\u6027\uff0c\u5206\u6790\u4e86\u53d8\u5f02\u7cfb\u6570 (CV%), \u7ed3\u679c\u663e\u793a\u5728\u591a\u91cd PCR \u548c \u5de2 \u5f0f PCR \u5b9e\u9a8c\u4e2d\uff0c OT-2 \u7684 CV% \u503c\u4e0e\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u76f8\u6bd4\u76f8\u540c\u6216\u8f83\u4f4e\uff0c\u8868\u660e\u6269\u589e\u7684\u5747\u5300\u6027\u66f4\u597d(\u89c1\u88681)\u3002<\/p>\n\n\n\n<p>\u88681:\u5728\u4e0d\u540c\u6a21\u677f\u7c7b\u578b\u548cPCR\u5b9e\u9a8c\u4e2d\u8868\u73b0\u51fa\u9ad8\u5ea6\u5747\u5300\u7684\u6269\u589e\u6548\u679c\u3002<br>CV\u503c(\u53d8\u5f02\u7cfb\u6570)\u4e3a\u7ecf\u8fc7Qubit \u5b9a\u91cf\u540e\u8ba1\u7b97\u5f97\u51fa\u7684\u7ed3\u679c\uff0c\u5206\u522b\u663e\u793a\u4e86\u5728OT-2 \u548c\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u8fdb\u884c\u7684\u6bcf\u4e2a\u5b9e\u9a8c\u7684CV\u503c\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"495\" height=\"255\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-73.png\" alt=\"\u00a0PCR\u00a0\u81ea\u52a8\u5316\u5b9e\u9a8c\" class=\"wp-image-3011\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-73.png 495w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-73-300x155.png 300w\" sizes=\"(max-width: 495px) 100vw, 495px\" \/><figcaption class=\"wp-element-caption\"><strong>\u5728<\/strong>\u00a0<strong>OT-2<\/strong>\u00a0<strong>\u4e0a\u8fdb\u884c\u5de2\u5f0f<\/strong>\u00a0<strong>PCR<\/strong>\u00a0<strong>\u81ea\u52a8\u5316\u5b9e\u9a8c\u4ea7\u751f\u7684<\/strong>\u00a0<strong>PCR<\/strong>\u00a0<strong>\u6269\u589e\u4ea7\u7269\u4e0e\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u7684\u4ea7\u7269\u76f8\u4f3c\u3002<\/strong><\/figcaption><\/figure>\n\n\n\n<p>\u5728\u5de2\u5f0f\u00a0PCR\u00a0\u7684\u5e03\u5c40\u4e2d\uff0c\u6837\u54c1\u5b54\u4ee5\u7eff\u8272\u6807\u8bb0\uff0c\u9634\u6027\u5bf9\u7167\u5b54\u4ee5\u84dd\u8272\u6807\u8bb0(\u56fe3)<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"660\" height=\"200\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-74.png\" alt=\"\u5b9e\u9a8c\u7ed3\u679c\u5bf9\u7167\" class=\"wp-image-3012\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-74.png 660w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-74-300x91.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-74-576x175.png 576w\" sizes=\"(max-width: 660px) 100vw, 660px\" \/><\/figure>\n\n\n\n<p>\u8bbe\u8ba1\u4e86\u4e24\u7ec4\u5f15\u7269\uff0c\u7528\u4e8e\u68c0\u6d4b Lambda&nbsp;&nbsp;\u6a21\u677f&nbsp;DNA&nbsp;\u7684500 bp&nbsp;\u548c247 bp&nbsp;\u533a\u57df\u3002\u7b2c\u4e00\u6b21PCR&nbsp;\u8fd0\u884c\u4e2d\u68c0\u6d4b\u5230\u4e86500&nbsp;bp&nbsp;\u533a\u57df\uff0c\u800c\u7b2c\u4e8c\u6b21&nbsp;PCR \u8fd0\u884c\u4e2d\u68c0\u6d4b\u5230\u4e86247bp&nbsp;&nbsp;\u533a\u57df\u3002\u6837\u54c1\u8fdb\u884c\u4e86\u4e09\u6b21\u5de2\u5f0f&nbsp;PCR&nbsp;\u91cd\u590d\u5b9e\u9a8c\uff0c\u7136\u540e\u5728&nbsp;PCR&nbsp;\u8fd0\u884c\u7ed3\u675f\u540e\u5bf9\u6269\u589e\u4ea7\u7269\u8fdb\u884c\u5206\u6790\u3002<\/p>\n\n\n\n<p>\u5728\u743c\u8102\u7cd6\u51dd\u80f6\u4e0a\u5206\u6790\u4e86\u5728\u00a0OT-2 \u00a0\u548c\u7b2c\u4e09\u65b9\u70ed\u5faa\u73af\u4eea\u4e0a\u751f\u6210\u7684\u00a0PCR\u6269\u589e\u4ea7\u7269(\u56fe4)\u3002\u5916\u5c42\u6269\u589e\u7247\u6bb5\u7684\u9884\u671f\u5927\u5c0f\u4e3a500 bp, \u00a0\u00a0\u00a0\u5185\u5c42\u6269\u589e\u7247\u00a0\u6bb5\u4e3a247 bp\u00a0\u3002PCR\u00a0\u6269\u589e\u4ea7\u7269\u663e\u793a\u51fa\u7c7b\u4f3c\u7684\u4ea7\u91cf\u548c\u53d8\u5f02\u7cfb\u6570 (CV%) \u00a0\u00a0\u00a0(\u88681),\u5b9e\u9a8c\u7ed3\u679c\u8bf4\u660e\u4e86\u00a0OT-2 \u00a0\u7684\u4ea7\u51fa\u4e0e\u624b\u5de5\u64cd\u4f5c\u548c\u7b2c\u4e09\u65b9\u8bbe\u5907\u00a0\u4e00\u81f4\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"983\" height=\"182\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-75.png\" alt=\"\u5b9e\u9a8c\u7ed3\u679c\" class=\"wp-image-3013\" style=\"width:840px;height:auto\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-75.png 983w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-75-300x56.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-75-768x142.png 768w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-75-576x107.png 576w\" sizes=\"(max-width: 983px) 100vw, 983px\" \/><\/figure>\n\n\n\n<p><strong>\u7ed3\u8bba<\/strong><\/p>\n\n\n\n<p><strong>Opentrons<\/strong>&nbsp;&nbsp;<strong>PCR<\/strong>&nbsp;<strong>\u5de5\u4f5c\u7ad9\u4e0e\u7b2c\u4e09\u65b9\u624b\u52a8\u70ed\u5faa\u73af\u4eea\u5bf9\u6bd4\uff0c\u5728<\/strong>&nbsp;<strong>PCR<\/strong>&nbsp;<strong>\u6269\u589e\u4ea7\u7269\u7684\u78b1\u57fa\u957f\u5ea6\u65b9\u9762\u8868\u73b0\u51fa\u7cbe\u786e\u7684\u6269\u589e\u80fd\u529b\uff0c\u5e76\u4e14\u79fb\u6db2\u7cbe\u51c6\u5ea6\u9ad8\u3002\u6b64<\/strong>&nbsp;&nbsp;<strong>\u5916\uff0c<\/strong><strong>Opentrons<\/strong>&nbsp;<strong>PCR<\/strong>&nbsp;<strong>\u5de5\u4f5c\u7ad9\u5c55\u793a\u4e86\u5728\u4e00<\/strong><strong>\u4e2a\u5b54\u4e2d\u51c6\u786e\u8fdb\u884c\u591a\u91cd<\/strong>&nbsp;<strong>PCR<\/strong>&nbsp;<strong>\u4ee5\u53ca\u5de2\u5f0f<\/strong>&nbsp;<strong>PCR<\/strong>&nbsp;<strong>\u5b9e\u9a8c\u7684\u6269\u589e\u80fd\u529b<\/strong>\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6458\u8981\u968f\u7740\u751f\u547d\u79d1\u5b66\u5c4a\u5bf9PCR\u7684\u9700\u6c42\u6b63\u5728\u589e\u52a0\u3002\u5728\u672c\u5e94\u7528\u8bf4\u660e\u4e2d\uff0c\u6211\u4eec\u5c55\u793a\u4e86 Opentrons PCR \u5de5\u4f5c\u7ad9\u4e0e\u5e02\u9762 [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":3013,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3008","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Opentrons PCR \u5de5\u4f5c\u7ad9\u81ea\u52a8\u5316PCR\u5236\u5907\u53cd\u5e94\u65b9\u6848 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/news\/opentrons-pcr-gzzzdhpcr\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Opentrons PCR \u5de5\u4f5c\u7ad9\u81ea\u52a8\u5316PCR\u5236\u5907\u53cd\u5e94\u65b9\u6848 - Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"og:description\" content=\"\u6458\u8981\u968f\u7740\u751f\u547d\u79d1\u5b66\u5c4a\u5bf9PCR\u7684\u9700\u6c42\u6b63\u5728\u589e\u52a0\u3002\u5728\u672c\u5e94\u7528\u8bf4\u660e\u4e2d\uff0c\u6211\u4eec\u5c55\u793a\u4e86 Opentrons PCR \u5de5\u4f5c\u7ad9\u4e0e\u5e02\u9762 [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/opentrons.com.cn\/news\/opentrons-pcr-gzzzdhpcr\/\" \/>\n<meta property=\"og:site_name\" content=\"Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"article:published_time\" content=\"2024-06-25T15:15:10+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-09-02T10:57:14+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-75.png\" \/>\n\t<meta property=\"og:image:width\" content=\"983\" \/>\n\t<meta property=\"og:image:height\" content=\"182\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Huang, Chris\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"Huang, Chris\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 \u5206\" \/>\n<script type=\"application\/ld+json\" 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