{"id":3098,"date":"2024-06-29T13:49:14","date_gmt":"2024-06-29T05:49:14","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3098"},"modified":"2024-09-02T18:29:35","modified_gmt":"2024-09-02T10:29:35","slug":"dyot-2gxzxhfxytcl","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/dyot-2gxzxhfxytcl\/","title":{"rendered":"\u5e26\u6709OT-2\u5149\u5b66\u4e2d\u5fc3\u7684\u6325\u53d1\u6027\u6db2\u4f53\u5904\u7406"},"content":{"rendered":"\n

\u4f5c\u8005
MorayoAdeyibi, Ph.D.<\/p>\n\n\n\n

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\u4e09\u79cd\u7a00\u91ca\u5ea6\u7684\u4e59\u9187\uff0c70%\uff0c80%\u548c99% (SigmaAldrich\uff0c\u76ee\u5f55\u7f16\u53f7752X\uff0cE-030,1.07017)\u4f5c\u4e3a\u5177\u6709\u4ee3\u8868\u6027\u7684\u6325\u53d1\u6027\u6db2\u4f53\u8fdb\u884c\u4e86\u4f53\u79ef\u5b9a\u91cf\u6d4b\u8bd5\uff0c\u5e76\u4e0e\u6c34\u8fdb\u884c\u4e86\u6bd4\u8f83\u3002\u91cd\u91cf\u6d4b\u91cf\u91c7\u7528\u5fae\u91cf\u5929\u5e73(Radwag\uff0cXA6\/21.4Y.M.A.P\u52a0\u4e0a\u5fae\u5e73\u8861\u5668)\u3002\u5bf94\u4e2a\u4e2d\u5fc3GEN2 P20\u3001P300\u548cP1000\u79fb\u6db2\u7ba1\u8fdb\u884c\u4e86\u4f53\u79ef\u6d4b\u8bd5\u3002\u89c6\u4e2d\u5fc3P20\u975e\u8fc7\u6ee4\u5668\uff0cP300\u975e\u8fc7\u6ee4\u5668\u548cP1000\u975e\u8fc7\u6ee4\u5668\u5c16\u7aef\u7528\u4e8e\u79fb\u6db2\u7684\u6325\u53d1\u6027\u6db2\u4f53\u3002\u6240\u6709\u6db2\u4f53\u90fd\u572822\u02da\u523025\u02da\u7684\u73af\u5883\u4e4b\u95f4\u8fdb\u884c\u6d4b\u8bd5\u6e29\u5ea6\u548c50% – 60%\u7684\u76f8\u5bf9\u6e7f\u5ea6. \u51cf\u5c11\u6bcf\u4e2a\u79fb\u6db2\u7ba1\u7684\u6700\u5927\u5438\u6c14\u91cf\uff0c\u4ee5\u6bd4\u8f83\u6c14\u9699\u6548\u5e94\u3002\u4f18\u5316\u53c2\u6570\u88681\u4e2d\u5217\u51fa\u7684\u662f\u7528\u4e8e\u91cd\u91cf\u8bbe\u7f6e\u4e0a\u7684\u4f53\u79ef\u6d4b\u8bd5\u3002<\/p>\n\n\n\n

\u6240\u6709\u53c2\u6570\u90fd\u4f7f\u7528\u4f7f\u7528\u5185\u90e8\u79fb\u6db2\u7ba1\u9a8c\u8bc1\u534f\u8bae\u7684\u4e2d\u5fc3Python API\u8fdb\u884c\u7f16\u7a0b\u3002\u8fd9\u4e2a\u5c16\u7aef\u5728\u6db2\u4f53\u5916\u505c\u7559\u4e86\u5927\u7ea615\u79d2\u949f\u3002\u75284\u4e2a\u4e0d\u540c\u7684\u5438\u6db2\u7ba1\u6536\u96c6\u6bcf\u4f53\u79ef\u5438\u6db2\u7ba110\u6b21\u91cd\u590d\u3002Gen2 P20\u79fb\u6db2\u7ba1\u7684\u76ee\u6807\u4f53\u79ef\u5206\u522b\u4e3a1\u00b5L\u300110\u00b5L\u300115\u00b5Land 18\u00b5L\u3002\u7b2c2\u4ee3P300\u4ee3\u7684\u76ee\u6807\u4f53\u79ef\u4e3a20\u00b5L\u3001100\u00b5L\u548c200\u00b5L\u3002\u7b2c2\u4ee3P1000\u4ee3\u7684\u76ee\u6807\u4f53\u79ef\u4e3a100\u00b5L\uff0c500\u00b5Land 950\u00b5L\u3002<\/p>\n\n\n\n

\u7ed3\u679c\u548c\u8ba8\u8bba
\u6d4b\u91cfOT-2\u79fb\u6db2\u7ba1\u7684\u6027\u80fd\u80fd\u529b\u4e0d\u51c6\u786e\u5730\u5904\u7406\u6700\u5e38\u7528\u7684\u4ee3\u8868\u6027\u6325\u53d1\u6027\u6db2\u4f53\uff0c\u5b9e\u9a8c\u76f8\u5173\u7814\u7a76\u4e86\u4e59\u9187\u3001\u5f02\u4e19\u9187\u3001\u4e19\u916e\u548c\u7532\u9187\u7684\u7a00\u91ca\u5ea6\u3002\u4e59\u9187\u548c\u5f02\u4e19\u9187\u5177\u6709\u76f8\u4f3c\u7684\u84b8\u6c7d\u538b\u8303\u56f4\uff0c\u56e0\u6b64\u6211\u4eec\u91c7\u7528\u4e86\u7c7b\u4f3c\u7684\u79fb\u4f4d\u53c2\u6570\u3002<\/p>\n\n\n\n

\u9884\u6da6\u6e7f\u589e\u52a0\u4e86\u5438\u6db2\u7ba1\u5185\u7684\u4f53\u79ef\uff0c\u56e0\u4e3a\u5728\u9884\u6da6\u6e7f\u4f5c\u7528\u5b8c\u6210\u540e\uff0c\u9884\u6da6\u6e7f\u9644\u7740\u5728\u5c16\u7aef\u8868\u9762\u7684\u4f53\u79ef\u3002\u9884\u6da6\u6e7f\u7684\u597d\u5904\u53ef\u4ee5\u770b\u4f5c\u662f\u964d\u4f4e\u4e86\u6325\u53d1\u6027\u6db2\u4f53\u7684\u6ef4\u843d\u91cf\u3002\u6ca1\u6709\u9884\u6da6\u6e7f\uff0c\u8f83\u9ad8\u6d53\u5ea6\u7684\u4e59\u9187\uff0c\u7279\u522b\u662f99%\u7684\u4e59\u9187\uff0c\u66f4\u5bb9\u6613\u53d1\u751f\u5373\u4f7f\u8fdb\u884c\u4e86\u5c3e\u968f\u7684\u6c14\u9699\u62bd\u5438\uff0c\u4e5f\u4f1a\u8fc5\u901f\u6ef4\u843d\u3002\u8fd9\u5c31\u5bfc\u81f4\u4e86\u5bf9\u5468\u56f4\u73af\u5883\u7684\u6c61\u67d3\u7532\u677f\u548c\u65e0\u6cd5\u8fbe\u5230\u76ee\u6807\u5206\u914d\u91cf\u3002<\/p>\n\n\n\n

\u867d\u7136<10\u7684\u5438\u5165\u6027\u7528\u4f8b\u6709\u9650\u00b5L\u6325\u53d1\u6027\u6db2\u4f53\uff0c\u62bd\u5438\u548c\u5206\u914d\u53ef\u4ee5\u76ee\u524d\u7684\u6311\u6218\u3002\u5bf9\u8fd9\u4e9b\u4f4e\u4f53\u79ef\u7684\u9884\u6da6\u6e7f\u53ef\u80fd\u5bfc\u81f4\u8fc7\u5ea6\u5438\u5165\u3002\u7136\u800c\uff0c\u5728\u4f7f\u7528P20\u65f6\uff0c\u6240\u6709\u6d4b\u8bd5\u4f53\u79ef\u7684~\u5747\u89c2\u5bdf\u5230~1.5\u00b5L\u7684\u6b7b\u4f53\u79ef\u3002\u6b7b\u4f53\u79ef\u5305\u62ec\u5c16\u7aef\u4e2d\u7684\u84b8\u53d1\u6db2\u4f53\uff0c\u5269\u4f59\u7684\u6db2\u4f53\u4e3a\u7c98\u5728\u5c16\u7aef\u4e0a\u3002<\/p>\n\n\n\n

\u53cd\u5411\u79fb\u6db2\uff0c\u5176\u4e2d\u6db2\u4f53\u88ab\u9884\u5438\u4e3a\u6ee1\u4f53\u79ef\uff0c\u800c\u6ca1\u6709\u76ee\u6807\u4f53\u79ef\uff0c\u5bf9\u4e8e\u9ad8\u84b8\u6c7d\u538b\u7684\u6db2\u4f53\u4e5f\u4f1a\u5f71\u54cd\u51c6\u786e\u6027\u3002\u66f4\u9ad8\u7684\u6c14\u9699\u4f53\u79ef\u53ef\u4ee5\u5e2e\u52a9\u51cf\u5c11\u8fd9\u79cd\u5f71\u54cd\u3002\u7531\u4e8e\u7531\u4e8e\u6c14\u9699\u4f53\u79ef\u7684\u635f\u5931\u800c\u9700\u8981\u5206\u914d\u7684\u6db2\u4f53\u91cf\u5c1a\u4e0d\u6e05\u695a\uff0c\u56e0\u6b64\u53cd\u5411\u79fb\u6db2\u53ef\u80fd\u4f1a\u5bfc\u81f4\u989d\u5916\u7684\u4e0d\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n

\u7ed3\u8bba
GEN2 P300\u548cP1000\u7684\u7cbe\u5ea6\u4e0e\u6c34\u5bf9\u79fb\u6db2\u7ba1\u7684\u6027\u80fd\u76f8\u4f3c\u3002\u9884\u6da6\u6e7f\u6b65\u9aa4\u7684\u5f71\u54cd\u4f1a\u5bfc\u81f4\u8fc7\u5ea6\u5438\u5165\u5728P20\u7528\u4e8e\u6325\u53d1\u6027\u6db2\u4f53\u5904\u7406\u7684\u60c5\u51b5\u4e0b\uff0c\u5efa\u8bae\u5bf9\u6240\u9700\u7684\u76ee\u6807\u8fdb\u884c\u9884\u8bc4\u4f30\u5bf9\u4e8e\u8be5\u534f\u8bae\u7684\u4f53\u79ef\uff0c\u5e76\u8003\u8651\u52301\u52302\u00b5L\u6b7b\u5bb9\u91cf\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec\u7684\u652f\u6301\u6839\u636e\u60a8\u7684\u534f\u8bae\u63d0\u51fa\u9002\u5f53\u7684\u5efa\u8bae\u3002\u6240\u6709\u4f18\u5316\u53c2\u6570\u90fd\u662f\u9488\u5bf9\u6240\u8ba8\u8bba\u7684\u73af\u5883\u56e0\u7d20\uff0c\u4efb\u4f55\u6e29\u5ea6\u6216\u6e7f\u5ea6\u7684\u53d8\u5316\u90fd\u53ef\u80fd\u5f71\u54cd\u6240\u8ba8\u8bba\u7684\u53c2\u6570\u3002
\u5728\u5904\u7406\u84b8\u6c7d\u538b\u8f83\u9ad8\uff0c\u6839\u636e\u73af\u5883\u6e29\u5ea6\u5927\u4e8e30 kPa\u7684\u6db2\u4f53\u65f6\uff0c\u6211\u4eec\u5efa\u8bae\u589e\u52a0\u9884\u6da6\u6e7f\u6df7\u5408\u7269\uff08>2\u500d\uff09\uff0c\u589e\u52a0\u9002\u5f53\u7684\u6c14\u9699\u4f53\u79ef\u548c\u51cf\u5c11\u6d41\u91cf\uff0c\u4ee5\u5b9e\u73b0\u6709\u6548\u7684\u6db2\u4f53\u8f6c\u79fb\u3002
\u6b7b\u4f53\u79ef\u53d6\u51b3\u4e8e\u84b8\u6c7d\u538b\u6325\u53d1\u6027\u6db2\u4f53\uff0c\u79fb\u6db2\u7ba1\u7684\u4f53\u79ef(\u4f8b\u5982\u3002P20\uff0cP300\u6216P100)\uff0c\u5c16\u7aef\u5927\u5c0f\u548c\u5206\u914d\u65f6\u5c16\u7aef\u6d78\u6ce1\u6df1\u5ea6\u3002\u5728\u5206\u914d\u671f\u95f4\u6d78\u6ce1\u5c16\u7aef<2.5 mm\u4f1a\u964d\u4f4e\u6b7b\u4f53\u79ef\u3002\u84b8\u6c7d\u538b\u8f83\u4f4e\u3001\u5bc6\u5ea6\u8f83\u9ad8\u7684\u6db2\u4f53\u7684\u6b7b\u4f53\u79ef\u8f83\u5c0f\u3002\u5982\u679c\u534f\u8bae\u4e2d\u4e0d\u9700\u8981\u89e6\u5934\u6216\u4e95\u55b7\uff0c\u5efa\u8bae\u6839\u636e\u6b7b\u4f53\u79ef\u8003\u8651\u76ee\u6807\u4f53\u79ef\u3002\u7ed3\u679c\u8868\u660e\uff0c\u6325\u53d1\u6027\u6db2\u4f53\u5904\u7406\u6027\u80fd\u4f18\u5316\u540e\u7684\u6325\u53d1\u6027\u6db2\u4f53\u5904\u7406\u53c2\u6570\u3002\u7528\u6237\u5c06\u80fd\u591f\u6839\u636e\u534f\u8bae\u4e2d\u4f7f\u7528\u7684\u7279\u5b9a\u6325\u53d1\u6027\u6db2\u4f53\u81ea\u5b9a\u4e49\u53c2\u6570\u3002<\/p>\n\n\n\n

\"\u6325\u53d1\u6027\u6db2\u4f53\u7684\u4f18\u5316\u7b56\u7565\"
\u56fe2 (a).<\/strong>\u6325\u53d1\u6027\u6db2\u4f53\u7684\u4f18\u5316\u7b56\u7565\u3002<\/em><\/figcaption><\/figure>\n\n\n\n
\"\u5206\u914d\u6325\u53d1\u6027\u6db2\u4f53\u7684\u4f18\u5316\u7b56\u7565\"
\u56fe2 (b).<\/strong>\u5206\u914d\u6325\u53d1\u6027\u6db2\u4f53\u7684\u4f18\u5316\u7b56\u7565\u3002<\/em><\/figcaption><\/figure>\n\n\n\n
\"P20\u5438\u5165\uff08\u5de6\uff09\u548c\u5206\u914d\uff08\u53f3\uff09\u7684\u4e0d\u540c\u6db2\u4f53\u7684\u6bd4\u8f83\"
\u56fe3.P20\u5438\u5165\uff08\u5de6\uff09\u548c\u5206\u914d\uff08\u53f3\uff09\u7684\u4e0d\u540c\u6db2\u4f53\u7684\u6bd4\u8f83\u3002<\/figcaption><\/figure>\n\n\n\n
\"\u9ad8\u7cbe\u5ea6\u6325\u53d1\u6027\u6db2\u4f53\u5438\u5165\uff08\u5de6\uff09\u548c\u4f7f\u7528GEN2
\u56fe4\uff1a\u9ad8\u7cbe\u5ea6\u6325\u53d1\u6027\u6db2\u4f53\u5438\u5165\uff08\u5de6\uff09\u548c\u4f7f\u7528GEN2 P300\u79fb\u6db2\u7ba1\u5206\u914d\uff08\u53f3\uff09\u3002<\/em><\/figcaption><\/figure>\n\n\n\n
\"\u9ad8\u7cbe\u5ea6\u6325\u53d1\u6027\u6db2\u4f53\u5438\u5165\uff08\u5de6\uff09\u548c\u7528GEN2
\u56fe5\uff1a\u9ad8\u7cbe\u5ea6\u6325\u53d1\u6027\u6db2\u4f53\u5438\u5165\uff08\u5de6\uff09\u548c\u7528GEN2 P1000\u79fb\u6db2\u7ba1\u5206\u914d\uff08\u53f3\uff09\u3002<\/em><\/figcaption><\/figure>\n\n\n\n
\"\u5b9e\u9a8c\u7ed3\u679c\u88681\"<\/figure>\n\n\n\n
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