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\u5b8c\u6574\u7ec4\u88c5\u7684\u7cfb\u7edf\u5982\u56fe1A\u6240\u793a\u3002\u786c\u4ef6\u7531\u51e0\u4e2a\u4e3b\u8981\u6a21\u5757\u7ec4\u6210\uff1a\u6c34\u5e73\u79fb\u52a8\u591a\u5b54\u677f\u7684 XY \u5e73\u53f0\uff1b\u5e26\u6709\u81ea\u52a8\u5bf9\u7126\u7cfb\u7edf\u7684\u5c0f\u578b\u8367\u5149\u663e\u5fae\u955c\uff08\u8865\u5145\u89c6\u9891 1\uff09\uff1b\u4ee5\u53ca\u5c06\u516b\u79cd\u6eb6\u6db2\u5e94\u7528\u5230\u5404\u4e2a\u5b54\u4e2d\u7684\u704c\u6ce8\u6b67\u7ba1\uff08\u8865\u5145\u89c6\u9891 2\uff09\u3002\u6784\u5efa\u8bf4\u660e\u548c\u6240\u6709 3D \u6253\u5370\u6587\u4ef6\u53ef\u5728https:\/\/github.com\/frescolabs\/FrescoM\u83b7\u5f97\uff08\u53e6\u89c1\u8865\u5145\u6587\u672c\uff09\u3002\u53ef\u4ee5\u4f7f\u7528 Python \u7f16\u5199\u7684\u7528\u6237\u754c\u9762\u64cd\u4f5c\u786c\u4ef6\uff08\u56fe 1B\uff0c\u6709\u5173\u8be6\u7ec6\u4fe1\u606f\uff0c\u8bf7\u53c2\u9605\u201c\u8f6f\u4ef6\u201d\u90e8\u5206\uff09\uff0c\u8be5\u754c\u9762\u63a7\u5236\u5e73\u53f0\u3001\u7269\u955c\u548c\u6b67\u7ba1\u4ee5\u53ca\u81ea\u52a8\u5bf9\u7126\u3001\u66dd\u5149\u3001\u7167\u660e\u548c\u5b9e\u9a8c\u65b9\u6848\u7684\u6574\u4f53\u7ba1\u7406\u3002<\/p>\n\n\n\n

\"\u8367\u5149\u62a5\u544a\u57fa\u56e0\u6210\u50cf\"
\u56fe 1.<\/strong>\u6240\u5f00\u53d1\u5e73\u53f0\u7684\u5b9e\u65bd\u53ca\u5176\u5728\u8367\u5149\u62a5\u544a\u57fa\u56e0\u6210\u50cf\u4e2d\u7684\u9002\u7528\u6027\u3002
\uff08A\uff09<\/strong>\u6240\u5f00\u53d1\u7684\u786c\u4ef6\u548c\u7535\u8def\u677f\u3002\u5173\u952e\u6a21\u5757\u4ee5\u7ea2\u8272\u6807\u51fa\u3002
\uff08B\uff09<\/strong>\u786c\u4ef6\u64cd\u4f5c\u8f6f\u4ef6\u3002\u9876\u90e8\u6309\u94ae\u64cd\u4f5c\u591a\u5b54\u677f\u3001\u704c\u6ce8\u6b67\u7ba1\u548c\u805a\u7126\u7cfb\u7edf\u7684\u79fb\u52a8\u3002\u4e2d\u95f4\u4e00\u7ec4\u6309\u94ae\u9a71\u52a8\u6240\u6709\u7535\u673a\u79fb\u52a8\u5230\u96f6\u4f4d\u3002\u5e95\u90e8\u6309\u94ae\u64cd\u4f5c\u6444\u50cf\u5934\u3001\u767d\u8272\u548c\u84dd\u8272 LED \u548c\u6cf5\uff0c\u5e76\u8fd0\u884c\u9009\u5b9a\u7684\u534f\u8bae\u3002
\uff08C\u3001D\uff09<\/strong>\u5355\u4e2a\u9499\u6210\u50cf\u5b9e\u9a8c\u7684\u793a\u4f8b\u3002\u7ec6\u80de\u81ea\u52a8\u7528 Fluo4-AM \u9499\u67d3\u6599\u6807\u8bb0\uff0c\u5e76\u5728\u5e94\u7528 100 \u03bcm ATP \u4e4b\u524d\u548c\u4e4b\u540e\u8fdb\u884c\u6210\u50cf\u3002\u53f3\u56fe\u663e\u793a\u4f7f\u7528 Cellpose \u7b97\u6cd5\u7684\u7ec6\u80de\u5206\u5272\uff08\u7ec6\u80de\u8fb9\u754c\u4ee5\u7ea2\u8272\u7a81\u51fa\u663e\u793a\uff09\u3002
\uff08E\u3001F\uff09<\/strong>\u76f8\u540c\u7684\u9499\u6210\u50cf\u5b9e\u9a8c\u91cd\u590d 30 \u6b21\uff0c\u5e76\u8ba1\u7b97\u6bcf\u4e2a\u5355\u72ec\u5b54\u4e2d\u591a\u4e2a\u7ec6\u80de\u7684\u5e73\u5747\u54cd\u5e94\u5e76\u663e\u793a\u5728\u56fe
(E)<\/strong>\u4e2d\u3002\u5e94\u7528 100 \u03bcm ATP \u53ef\u5f3a\u70c8\u5f15\u8d77\u6240\u6709\u5b54\u4e2d\u9499\u6d53\u5ea6\u7684\u5347\u9ad8\u3002\u9762\u677f
(C\u3001E)<\/strong>\u4e2d\u7684\u6bd4\u4f8b\u5c3a
\u4e3a 100 \u03bcm\u3002<\/figcaption><\/figure>\n\n\n\n

\u4e3a\u4e86\u5c55\u793a\u8be5\u5e73\u53f0\uff0c\u6211\u4eec\u9996\u5148\u6f14\u793a\u4e86\u5b83\u5982\u4f55\u7528\u4e8e\u5927\u89c4\u6a21\u8367\u5149\u6210\u50cf\u5b9e\u9a8c\uff08Nasu et al., 2021\uff09\u3002\u6d89\u53ca\u8367\u5149\u62a5\u544a\u57fa\u56e0\u7684\u5178\u578b\u5b9e\u9a8c\u65b9\u6848\u9700\u8981\u5728\u5e94\u7528\u6fc0\u52a8\u5242\u3001\u751f\u957f\u56e0\u5b50\u6216\u5176\u4ed6\u6d3b\u6027\u5316\u5408\u7269\u4e4b\u524d\u548c\u4e4b\u540e\u5bf9\u7ec6\u80de\u8fdb\u884c\u4e00\u6bb5\u65f6\u95f4\u7684\u6210\u50cf\u3002\u4e3a\u4e86\u8bc1\u660e\u5f00\u53d1\u7684\u5b9e\u9a8c\u5e73\u53f0\u5bf9\u8fd9\u79cd\u5b9e\u9a8c\u8303\u5f0f\u7684\u53ef\u7528\u6027\uff0c\u6211\u4eec\u4f7f\u7528\u5408\u6210\u7684\u9499\u6d53\u5ea6\u8367\u5149\u6307\u793a\u5242\u8fdb\u884c\u4e86\u9499\u6210\u50cf\u3002\u7ec6\u80de\u81ea\u52a8\u7528\u8367\u5149\u9499\u67d3\u6599 Fluo4-AM \u6807\u8bb0\uff08\u56fe 1C\uff09\uff0c\u5e76\u54cd\u5e94 100 \u03bcm ATP \u8fdb\u884c\u9499\u6210\u50cf\u3002\u7136\u540e\u4f7f\u7528 Cellpose \u7b97\u6cd5\uff08Stringer et al., 2021 \uff09\u81ea\u52a8\u5206\u5272\u7ec6\u80de\uff08\u56fe 1C \uff0c\u53f3\uff09 \uff0c\u5e76\u63d0\u53d6\u5355\u4e2a\u7ec6\u80de\u7684\u8367\u5149\u52a8\u529b\u5b66\uff08\u56fe 1D\uff09\u3002\u7136\u540e\u5728\u5176\u4ed6 30 \u4e2a\u5b54\u4e2d\u81ea\u52a8\u91cd\u590d\u76f8\u540c\u7684\u5b9e\u9a8c\u7a0b\u5e8f\u548c\u5206\u6790\uff08\u56fe 1E\u3001F\u548c\u8865\u5145\u89c6\u9891 3\uff09\u3002\u8fd9\u4e9b\u5b9e\u9a8c\u8868\u660e\uff0c\u5f00\u53d1\u7684\u5e73\u53f0\u53ef\u4ee5\u5b9e\u73b0\u8367\u5149\u62a5\u544a\u57fa\u56e0\u7684\u7a33\u5065\u548c\u81ea\u52a8\u9ad8\u901a\u91cf\u6210\u50cf\u3002<\/p>\n\n\n\n

\u6240\u5f00\u53d1\u7cfb\u7edf\u7684\u53e6\u4e00\u4e2a\u91cd\u8981\u4f18\u52bf\u662f\u5b83\u5141\u8bb8\u5728\u5927\u91cf\u5b54\u4e2d\u5927\u89c4\u6a21\u751f\u6210\u56fe\u50cf\u3002 \u4e3a\u4e86\u8bc1\u660e\u8fd9\u79cd\u53ef\u7528\u6027\uff0c\u6211\u4eec\u751f\u6210\u4e86\u4e00\u4e2a Python \u534f\u8bae\u7c7b\uff08\u8865\u5145\u534f\u8bae 1\uff0c\u6709\u5173\u8be6\u7ec6\u4fe1\u606f\uff0c\u8bf7\u53c2\u9605\u201c\u8f6f\u4ef6\u201d\u90e8\u5206\uff09\uff0c\u4f7f\u5e73\u53f0\u5728\u6240\u6709 96 \u4e2a\u5b54\u4e0a\u79fb\u52a8\uff0c\u5bf9\u6bcf\u4e2a\u7ec6\u80de\u8fdb\u884c\u805a\u7126\uff0c\u5e76\u6355\u83b7\u660e\u573a\u6216\u8367\u5149\u56fe\u50cf\u3002 \u6b64\u7c7b\u5b9e\u9a8c\u7684\u793a\u4f8b\u5982\u56fe2A\u3001B\u6240\u793a\u3002 \u91cd\u8981\u7684\u662f\uff0c\u8be5\u7cfb\u7edf\u5141\u8bb8\u626b\u63cf\u5355\u4e2a\u5b54\u5e76\u5bf9\u540c\u4e00\u4e2a\u5b54\u751f\u6210\u591a\u5f20\u56fe\u50cf\uff08\u56fe 2C\uff09\uff0c\u8fd9\u5bf9\u4e8e\u5bfb\u627e\u7a00\u6709\u7ec6\u80de\uff08\u4f8b\u5982\uff0c\u6b63\u5728\u8fdb\u884c\u6709\u4e1d\u5206\u88c2\/\u51cb\u4ea1\u7684\u7ec6\u80de\u6216\u8f6c\u67d3\u6548\u7387\u4f4e\u4e0b\u7684\u9633\u6027\u7ec6\u80de\uff09\u5f88\u6709\u7528\u3002<\/p>\n\n\n\n

\"\u5c55\u793a\u4e86\u6240\u5f00\u53d1\u5e73\u53f0\u7684\u9002\u7528\u6027\"
\u56fe 2.\u66f4\u591a\u793a\u4f8b\u5c55\u793a\u4e86\u6240\u5f00\u53d1\u5e73\u53f0\u7684\u9002\u7528\u6027\u3002\uff08A\u3001B\uff09\u4ece\u591a\u4e2a\u5b54\u4e2d\u751f\u6210\u5927\u91cf\u56fe\u50cf\u3002\uff08C\uff09\u5355\u5b54\u626b\u63cf\u3002XY \u5e73\u53f0\u4f4d\u4e8e\u5355\u4e2a\u5b54\u7684\u4e2d\u5fc3\uff0c\u5e76\u4ece\u540c\u4e00\u5b54\u4e2d\u83b7\u53d6 HeLa \u7ec6\u80de\u7684\u591a\u5f20\u56fe\u50cf\u3002\uff08D\uff09\u4f7f\u7528\u9ea6\u82bd\u51dd\u96c6\u7d20\u81ea\u52a8\u6807\u8bb0 HeLa \u7ec6\u80de\u5e76\u968f\u540e\u81ea\u52a8\u6210\u50cf\u7684\u793a\u4f8b\u3002\u9762\u677f\u663e\u793a\u4e86\u4ece 48 \u4e2a\u4e0d\u540c\u5b54\u6210\u50cf\u7684\u793a\u4f8b\u3002\u81ea\u52a8\u5bf9\u7126\u662f\u5728\u767d\u5149\u4e0b\u8fdb\u884c\u7684\u3002\uff08E\uff09\u4f7f\u7528 Herceptin \u4f5c\u4e3a\u4e00\u6297\u548c Alexa 488 \u5076\u8054\u6297\u4eba\u6297\u4f53\u8fdb\u884c\u514d\u75ab\u67d3\u8272\u5b9e\u9a8c\u7684\u793a\u4f8b\u3002\u5728 SKBR3 \u7ec6\u80de\u4e2d\u89c2\u5bdf\u5230\u5f3a\u514d\u75ab\u6807\u8bb0\uff0c\u4f46\u5728\u53d7\u4f53\u6c34\u5e73\u76f8\u5bf9\u8f83\u4f4e\u7684 HeLa \u7ec6\u80de\u4e2d\u672a\u89c2\u5bdf\u5230\u3002\u6bd4\u4f8b\u5c3a\u4e3a 100 \u03bcm\u3002<\/figcaption><\/figure>\n\n\n\n

\u4e3a\u4e86\u8bc1\u660e\u6240\u5f00\u53d1\u7684\u5b9e\u9a8c\u5e73\u53f0\u5728\u6807\u8bb0\u5b9e\u9a8c\u4e2d\u7684\u53ef\u7528\u6027\uff0c\u6211\u4eec\u4f7f\u7528\u4e86\u8367\u5149\u5c0f\u9ea6\u80da\u82bd\u51dd\u96c6\u7d20 (WGA)\uff0c\u8be5\u7269\u8d28\u53ef\u7a81\u51fa\u663e\u793a\u7ec6\u80de\u819c\u3002\u7528 PBS \u81ea\u52a8\u6e05\u6d17\u4e94\u6392\u5b54\uff08\u5171 48 \u4e2a\u5b54\uff09\uff0c\u7136\u540e\u52a0\u5165\u542b\u6709 5 mg\/ml \u8367\u5149 WGA \u7684\u6eb6\u6db2\u3002\u5ba4\u6e29\u4e0b\u653e\u7f6e 10 \u5206\u949f\u540e\uff0c\u7528 PBS \u6e05\u6d17 WGA\uff0c\u7136\u540e\u653e\u5165\u8367\u5149\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u3002\u5f97\u5230\u7684\u8367\u5149\u56fe\u50cf\u5982\u56fe2D\u6240\u793a\u3002\u81ea\u52a8\u6807\u8bb0\u53ef\u4ea7\u751f\u5177\u6709\u660e\u786e\u8d28\u819c\u7684\u7ec6\u80de\u7684\u6e05\u6670\u56fe\u200b\u200b\u50cf\uff0c\u4ece\u800c\u8868\u660e\u53ef\u4ee5\u4f7f\u7528\u6240\u5f00\u53d1\u7684\u5e73\u53f0\u81ea\u52a8\u6267\u884c\u5e38\u89c4\u6807\u8bb0\u7a0b\u5e8f\u3002<\/p>\n\n\n\n

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\u8fd9\u4e9b\u793a\u4f8b\u8bc1\u660e\u4e86\u6240\u5f00\u53d1\u7684\u5b9e\u9a8c\u5e73\u53f0\u5728\u4e0d\u540c\u7c7b\u578b\u7684\u7ec6\u80de\u751f\u7269\u5b66\u5b9e\u9a8c\u81ea\u52a8\u5316\u65b9\u9762\u7684\u5e7f\u6cdb\u53ef\u7528\u6027\u3002\u4e0b\u9762\uff0c\u6211\u4eec\u5c06\u8be6\u7ec6\u63cf\u8ff0\u8be5\u5e73\u53f0\u5e76\u5728\u4e00\u7cfb\u5217\u6d4b\u8bd5\u4e2d\u5c55\u793a\u5176\u6027\u80fd\u3002<\/p>\n\n\n\n

\u786c\u4ef6\u8bbe\u8ba1
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