{"id":3121,"date":"2024-07-01T11:58:25","date_gmt":"2024-07-01T03:58:25","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=3121"},"modified":"2024-09-02T18:28:23","modified_gmt":"2024-09-02T10:28:23","slug":"lzyyrnatqfapg","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/lzyyrnatqfapg\/","title":{"rendered":"\u4e24\u79cd\u7528\u4e8e SARS-CoV-2 \u68c0\u6d4b\u7684\u81ea\u52a8\u5316\u4f4e\u6210\u672c RNA \u63d0\u53d6\u65b9\u6848\u7684\u8bc4\u4f30"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">\u80cc\u666f<\/h3>\n\n\n\n<p>\u5df2\u7ecf\u8bc4\u4f30\u4e86\u4e24\u79cd\u7528\u4e8e\u4ece\u9f3b\u54bd\u62ed\u5b50\u4e2d\u9ad8\u901a\u91cf\u63d0\u53d6 RNA \u4ee5\u68c0\u6d4b SARS-CoV-2 \u7684\u81ea\u52a8\u5316\u5185\u90e8\u534f\u8bae\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u65b9\u6cd5<\/h3>\n\n\n\n<p>\u5728 10 \u5929\u5185\u6536\u96c6\u4e86 141 \u4e2a SARS-CoV-2 \u9633\u6027\u6837\u672c\u3002\u5185\u90e8\u534f\u8bae\u57fa\u4e8e\u78c1\u73e0\u63d0\u53d6\uff0c\u8bbe\u8ba1\u7528\u4e8e Opentrons OT-2\uff08OT-2<sub>\u5185\u90e8<\/sub>\uff09\u6db2\u4f53\u5904\u7406\u673a\u5668\u4eba\u6216 MagMAX&nbsp;<sup>TM<\/sup>&nbsp;Express-96 \u7cfb\u7edf\uff08MM<sub>\u5185\u90e8\uff09\u3002\u4e24\u79cd\u534f\u8bae\u5747\u4e0e\u4f7f\u7528 MagMAX&nbsp;<\/sub><sup>TM<\/sup>\u7cfb\u7edf\uff08MM<sub>\u8bd5\u5242\u76d2<\/sub>\uff09\u7684\u5546\u4e1a\u8bd5\u5242\u76d2\u5e76\u884c\u6d4b\u8bd5\u3002\u6838\u9178\u63d0\u53d6\u6548\u7387\u662f\u6839\u636e SARS-CoV-2 DNA \u9633\u6027\u5bf9\u7167\u8ba1\u7b97\u7684\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u7ed3\u679c<\/h3>\n\n\n\n<p>\u5728\u68c0\u6d4b\u9633\u6027\u6837\u672c\u65b9\u9762\uff0c\u5185\u90e8\u65b9\u6848\u548c\u5546\u7528\u8bd5\u5242\u76d2\u4e4b\u95f4\u6ca1\u6709\u53d1\u73b0\u663e\u8457\u5dee\u5f02\u3002MM \u8bd5\u5242<sub>\u76d2<\/sub>\u6548\u7387\u6700\u9ad8\uff0c\u5c3d\u7ba1<sub>\u5185\u90e8 MM \u8bd5\u5242\u76d2<\/sub>\u7684\u5e73\u5747 Ct \u503c\u4f4e\u4e8e\u5176\u4ed6\u4e24\u79cd\u8bd5\u5242\u76d2\u3002\u4e0e\u5546\u7528\u8bd5\u5242\u76d2\u76f8\u6bd4\uff0c\u5185\u90e8\u65b9\u6848\u6bcf\u63d0\u53d6 96 \u4e2a\u6837\u672c\u53ef\u8282\u7701 350 \u6b27\u5143\u81f3 400 \u6b27\u5143\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u7ed3\u8bba<\/h3>\n\n\n\n<p>\u6240\u8ff0\u534f\u8bae\u5229\u7528\u6613\u4e8e\u83b7\u5f97\u7684\u8bd5\u5242\u548c\u5f00\u6e90\u6db2\u4f53\u5904\u7406\u7cfb\u7edf\uff0c\u9002\u7528\u4e8e\u9ad8\u901a\u91cf\u8bbe\u65bd\u4e2d\u7684 SARS-CoV-2 \u68c0\u6d4b\u3002<\/p>\n\n\n\n<p><strong>\u5f15\u7528\uff1a<\/strong>&nbsp;L\u00e1zaro-Perona F\u3001Rodriguez-Antol\u00edn C\u3001Alguacil-Guill\u00e9n M\u3001Guti\u00e9rrez-Arroyo A\u3001Mingorance J\u3001Garc\u00eda-Rodriguez J \u7b49\u4eba\u3002 \uff082021\uff09\u8bc4\u4f30\u4e24\u79cd\u7528\u4e8e SARS-CoV-2 \u68c0\u6d4b\u7684\u81ea\u52a8\u5316\u4f4e\u6210\u672c RNA \u63d0\u53d6\u65b9\u6848\u3002 PLoS ONE 16(2): e0246302\u3002 https:\/\/doi.org\/10.1371\/journal.pone.0246302<\/p>\n\n\n\n<p><strong>\u7f16\u8f91\uff1a<\/strong>&nbsp;AM Abd El-Aty\uff0c\u57c3\u53ca\u5f00\u7f57\u5927\u5b66<\/p>\n\n\n\n<p><strong>\u6536\u7a3f\u65e5\u671f\uff1a<\/strong>&nbsp;2020 \u5e74 11 \u6708 5 \u65e5\uff1b<strong>\u63a5\u53d7\u65e5\u671f\uff1a<\/strong>&nbsp;2021 \u5e74 1 \u6708 15 \u65e5\uff1b<strong>\u53d1\u8868\u65e5\u671f\uff1a<\/strong>&nbsp;2021 \u5e74 2 \u6708 16 \u65e5<\/p>\n\n\n\n<p><strong>\u7248\u6743\u6240\u6709\uff1a<\/strong>\u00a0\u00a9 2021 L\u00e1zaro-Perona \u7b49\u4eba\u3002\u8fd9\u662f\u4e00\u7bc7\u5f00\u653e\u83b7\u53d6\u7684\u6587\u7ae0\uff0c\u6839\u636e\u77e5\u8bc6\u5171\u4eab\u7f72\u540d\u8bb8\u53ef\u6761\u6b3e\u5206\u53d1\uff0c\u5141\u8bb8\u5728\u4efb\u4f55\u5a92\u4f53\u4e2d\u4e0d\u53d7\u9650\u5236\u5730\u4f7f\u7528\u3001\u5206\u53d1\u548c\u590d\u5236\uff0c\u524d\u63d0\u662f\u6ce8\u660e\u539f\u4f5c\u8005\u548c\u51fa\u5904\u3002<\/p>\n\n\n\n<p><strong>\u6570\u636e\u53ef\u7528\u6027\uff1a<\/strong>\u6240\u6709\u76f8\u5173\u6570\u636e\u5747\u5728\u8bba\u6587\u53ca\u5176\u652f\u6301\u4fe1\u606f\u6587\u4ef6\u4e2d\u3002<\/p>\n\n\n\n<p><strong>\u8d44\u91d1\uff1a<\/strong>\u4f5c\u8005\u672a\u6536\u5230\u6b64\u9879\u5de5\u4f5c\u7684\u7279\u5b9a\u8d44\u91d1\u8d44\u52a9\u3002<\/p>\n\n\n\n<p><strong>\u7ade\u4e89\u5229\u76ca\uff1a<\/strong>\u4f5c\u8005\u5df2\u58f0\u660e\u4e0d\u5b58\u5728\u7ade\u4e89\u5229\u76ca\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u4ecb\u7ecd<\/h2>\n\n\n\n<p>SARS-CoV-2 \u75ab\u60c5\u8981\u6c42\u5927\u89c4\u6a21\u4f7f\u7528 qPCR \u68c0\u6d4b\uff0c\u4ee5\u53d1\u73b0\u9633\u6027\u75c5\u4f8b\u5e76\u8ffd\u8e2a\u63a5\u89e6\u8005\uff0c\u4ece\u800c\u963b\u6b62\u793e\u533a\u4f20\u64ad\u3002\u5728 qPCR \u68c0\u6d4b\u4e4b\u524d\uff0c\u901a\u5e38\u9700\u8981\u5bf9\u4e34\u5e8a\u6837\u672c\u8fdb\u884c RNA \u63d0\u53d6 [\u00a01-4\u00a0]\u3002\u8003\u8651\u5230\u6bcf\u5929\u68c0\u6d4b\u7684\u6837\u672c\u6570\u91cf\uff0c\u5927\u591a\u6570\u673a\u6784\u65e0\u6cd5\u91c7\u7528\u624b\u52a8 RNA \u63d0\u53d6\u65b9\u6cd5\uff0c\u56e0\u6b64\uff0c\u81ea\u52a8\u5316\u7cfb\u7edf\u88ab\u5e7f\u6cdb\u7528\u4e8e\u6b64\u4efb\u52a1 [\u00a05-7\u00a0] \u3002\u81ea\u52a8\u5316\u7cfb\u7edf\u7684\u7f3a\u70b9\u662f\uff0c\u5b83\u663e\u8457\u589e\u52a0\u4e86\u6700\u7ec8\u6210\u672c\uff0c\u8fd9\u53ef\u80fd\u4f1a\u963b\u788d\u67d0\u4e9b\u5730\u533a\u7684\u5927\u89c4\u6a21\u68c0\u6d4b\u3002\u6b64\u5916\uff0c\u7531\u4e8e\u9700\u6c42\u589e\u52a0\uff0c\u63d0\u53d6\u8bd5\u5242\u7684\u5e93\u5b58\u77ed\u7f3a\u5bfc\u81f4\u8bca\u65ad\u4e25\u91cd\u5ef6\u8fdf\u3002<a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0246302#pone.0246302.ref004\"><\/a><a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0246302#pone.0246302.ref005\"><\/a><a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0246302#pone.0246302.ref007\"><\/a><\/p>\n\n\n\n<p>\u672c\u6587\u4ecb\u7ecd\u4e86\u4e24\u79cd\u4f4e\u6210\u672c\u7684\u81ea\u52a8 RNA \u63d0\u53d6\u65b9\u6cd5\u3002\u7b2c\u4e00\u79cd\u65b9\u6cd5\u4f7f\u7528 OT-2 \u7cfb\u7edf\uff08Opentrons\uff0c\u7ebd\u7ea6\u5dde\u7ebd\u7ea6\u5e02\uff0c\u7f8e\u56fd\uff09\uff0c\u8fd9\u662f\u4e00\u79cd\u80fd\u591f\u81ea\u52a8\u6267\u884c\u81ea\u884c\u8bbe\u8ba1\u65b9\u6848\u7684\u5f00\u6e90\u6db2\u4f53\u5904\u7406\u673a\u5668\u4eba\uff1b\u7b2c\u4e8c\u79cd\u65b9\u6cd5\u4f7f\u7528\u5feb\u901f\u4e14\u6613\u4e8e\u4f7f\u7528\u7684\u6838\u9178\u63d0\u53d6\u4eea MagMAX&nbsp;<sup>TM<\/sup>&nbsp;Express-96 \u7cfb\u7edf\uff08Thermo Fisher Scientific\uff0c\u9a6c\u8428\u8bf8\u585e\u5dde\u6c83\u5c14\u745f\u59c6\uff0c\u7f8e\u56fd\uff09\u3002\u540e\u8005\u53ef\u5728 30 \u5206\u949f\u5185\u63d0\u53d6\u591a\u8fbe 96 \u4e2a\u6837\u672c\uff0c\u4f46\u9700\u8981\u4e8b\u5148\u624b\u52a8\u5206\u914d\u8bd5\u5242\u3001\u78c1\u73e0\u548c 96 \u5b54\u677f\u4e2d\u7684\u6837\u672c\uff0c\u8fd9\u4f1a\u589e\u52a0 30 \u5206\u949f\u3002\u4f5c\u4e3a\u66ff\u4ee3\u65b9\u6848\uff0cOT-2<sub>\u5185\u90e8<\/sub>\u65b9\u6848\u53ef\u4ee5\u5b8c\u5168\u81ea\u52a8\u5316\u5730\u5728 104 \u5206\u949f\u5185\u5904\u7406\u591a\u8fbe 48 \u4e2a\u6837\u672c\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u65b9\u6cd5<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">\u6837\u54c1\u91c7\u96c6<\/h3>\n\n\n\n<p>\u5728\u5341\u5929\u5185\uff0c\u6536\u96c6\u4e86 141 \u4efd\u8fde\u7eed\u7684 SARS-CoV-2 \u9633\u6027\u9f3b\u54bd\u62ed\u5b50\uff0c\u8fd9\u4e9b\u62ed\u5b50\u5e26\u6709\u75c5\u6bd2\u8fd0\u8f93\u57f9\u517b\u57fa\uff08\u897f\u73ed\u7259\u5df4\u585e\u7f57\u90a3 Deltalab\uff09\uff0c\u5e76\u50a8\u5b58\u5728 4\u00b0C \u4e0b\u3002\u5904\u7406\u524d\uff0c\u5c06 500 \u03bcL \u75c5\u6bd2\u57f9\u517b\u57fa\u548c 500 \u03bcL 4M \u5f02\u786b\u6c30\u9178\u80cd (GTC)\uff08\u5fb7\u56fd\u5e0c\u5c14\u767b Qiagen\uff09\u4e0e 5 \u03bcg\/mL \u8f7d\u4f53 RNA \u6df7\u5408\uff0c\u4f7f\u6837\u54c1\u5931\u6d3b\uff0c\u7136\u540e\u5c06\u6837\u54c1\u5728 80\u00b0C \u4e0b\u52a0\u70ed 2 \u5206\u949f\uff0c\u5e76\u77ed\u6682\u6da1\u65cb\u6df7\u5408\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u8bbe\u5907\u548c\u8bd5\u5242<\/h3>\n\n\n\n<p>\u6838\u9178\u81ea\u52a8\u63d0\u53d6\u91c7\u7528\u4e24\u79cd\u7cfb\u7edf\uff1aMagMAX&nbsp;<sup>TM<\/sup>&nbsp;Express-96 \u6df1\u5b54\u78c1\u7c89\u5904\u7406\u5668\uff08King Fisher Instrument\uff0cThermo Fisher Scientific\uff0c\u7f8e\u56fd\u9a6c\u8428\u8bf8\u585e\u5dde\u6c83\u5c14\u745f\u59c6\uff09\u548c\u5f00\u653e\u5f0f\u7cfb\u7edf OT-2\uff08Opentrons\uff0c\u7f8e\u56fd\u7ebd\u7ea6\u5dde\u7ebd\u7ea6\uff09\uff0c\u914d\u6709 GEN1 \u78c1\u6a21\u5757\uff08Opentrons\uff0c\u7f8e\u56fd\u7ebd\u7ea6\u5dde\u7ebd\u7ea6\uff09\u548c\u5185\u90e8\u534f\u8bae\u3002\u4e24\u79cd\u7cfb\u7edf\u5747\u4f7f\u7528 MagMAX\u2122 Express 96 \u677f\u548c\u6df1\u5b54\u677f\uff08Thermo Fisher Scientific\uff0c\u7f8e\u56fd\u9a6c\u8428\u8bf8\u585e\u5dde\u6c83\u5c14\u745f\u59c6\uff09\u3002<a><\/a><\/p>\n\n\n\n<p>\u6838\u9178\u63d0\u53d6\u91c7\u7528\u4e09\u79cd\u65b9\u6cd5\uff1a1\uff09\u6839\u636e\u5236\u9020\u5546\u7684\u8bf4\u660e\uff0c\u4f7f\u7528 MagMAX\u00a0<sup>TM<\/sup>\u548c\u5546\u7528 MagMAX CORE \u6838\u9178\u7eaf\u5316\u8bd5\u5242\u76d2 (MM<sub>\u8bd5\u5242\u76d2)\uff08Thermo Fisher Scientific\uff0c\u7f8e\u56fd\u9a6c\u8428\u8bf8\u585e\u5dde\u6c83\u5c14\u745f\u59c6\uff09\uff1b2\uff09OT-2 \u7cfb\u7edf\u91c7\u7528\u901a\u7528\u8bd5\u5242\uff08OT-2\u5185\u90e8<\/sub>\uff09\uff0c\u4f8b\u5982\u4e59\u9187\uff08Emsure\u00ae\uff0cMerck KGaA\uff0c\u5fb7\u56fd\u8fbe\u59c6\u65bd\u5854\u7279\uff09\u30012-\u4e19\u9187\uff08Emsure\u00ae\uff0cMerck KGaA\uff0c\u5fb7\u56fd\u8fbe\u59c6\u65bd\u5854\u7279\uff09\u3001\u6d17\u8131\u7f13\u51b2\u6db2\uff08Omega BIO-TEK\uff0c\u7f8e\u56fd\u4f50\u6cbb\u4e9a\u5dde\u8bfa\u514b\u7f57\u65af\uff09\u3001\u65e0\u6838\u9178\u9176\u6c34\uff08Ambion\u00a0<sup>TM<\/sup>\uff0cThermo Fisher Scientific\uff0c\u7f8e\u56fd\u9a6c\u8428\u8bf8\u585e\u5dde\u6c83\u5c14\u745f\u59c6\uff09\u548c\u78c1\u73e0\uff08Mag-Bind\u00ae TotalPure NGS\uff0cOmega Bio-Tek\uff0c\u7f8e\u56fd\u4f50\u6cbb\u4e9a\u5dde\u8bfa\u514b\u7f57\u65af\uff09\uff1b3\uff09MagMAX\u00a0<sup>TM<\/sup>\u91c7\u7528\u4e0e\u5546\u7528\u8bd5\u5242\u76d2\u76f8\u540c\u7684\u534f\u8bae\uff0c\u4f46\u4f7f\u7528 OT-2 \u65b9\u6cd5\u4e2d\u7684\u8bd5\u5242\uff08MM<sub>\u5185\u90e8<\/sub>\uff09\u3002\u5185\u90e8\u534f\u8bae\u662f Hui He \u7b49\u4eba\u63cf\u8ff0\u7684\u7a0b\u5e8f\u7684\u4fee\u6539\u7248[8]\u3002\u7b80\u800c\u8a00\u4e4b\uff0c\u5c06\u706d\u6d3b\u7684\u547c\u5438\u9053\u6837\u672c\u4ee5 1:1 \u7684\u6bd4\u4f8b\u4e0e\u5f02\u4e19\u9187\u6df7\u5408\uff0c\u81f3\u6700\u7ec8\u4f53\u79ef\u4e3a 500 \u03bcl\uff08<sub>\u5185\u90e8OT-2\uff09\u548c 1000 \u03bcL\uff08\u5185\u90e8<\/sub>MM\uff09\uff0c\u52a0\u5165 40 \u03bcL \u78c1\u73e0\u5e76\u5728\u5ba4\u6e29\u4e0b\u5b75\u80b2 5 \u5206\u949f\u3002\u63a5\u4e0b\u6765\uff0c\u7528\u78c1\u94c1\u5c06\u78c1\u73e0\u62c9\u5230\u7ba1\u7684\u4e00\u4fa7\u5e76\u5f03\u53bb\u4e0a\u6e05\u6db2\u3002\u7136\u540e\u7528 500 \u03bcL \u65b0\u9c9c\u5236\u5907\u7684 70% \u4e59\u9187\u6d17\u6da4\u78c1\u73e0\u4e24\u6b21\u3002\u7b2c\u4e8c\u6b21\u6d17\u6da4\u540e\uff0c\u5f03\u53bb 70% \u4e59\u9187\u5e76\u5728\u5ba4\u6e29\u4e0b\u98ce\u5e72\u78c1\u73e0\u3002\u6700\u540e\uff0c\u5c06\u73e0\u5b50\u91cd\u65b0\u60ac\u6d6e\u5728 100 \u03bcL \u6d17\u8131\u7f13\u51b2\u6db2\u4e2d\u5e76\u518d\u6b21\u7528\u78c1\u94c1\u5206\u79bb\u4ee5\u56de\u6536\u6d17\u8131\u7684\u75c5\u6bd2 RNA\uff08\u88681\uff09\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/journals.plos.org\/plosone\/article\/figure\/image?size=medium&amp;id=10.1371\/journal.pone.0246302.t001\"><img decoding=\"async\" src=\"https:\/\/journals.plos.org\/plosone\/article\/figure\/image?size=inline&amp;id=10.1371\/journal.pone.0246302.t001\" alt=\"\u7f29\u7565\u56fe\"\/><\/a><\/figure>\n\n\n\n<p>\u8868 1.\u6240\u8bc4\u4f30\u7684\u4e09\u4e2a\u65b9\u6848\u4e2d\u4f7f\u7528\u7684\u6b65\u9aa4\u3001\u8bd5\u5242\u548c\u4f53\u79ef\uff08\u03bcL\uff09\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u534f\u8bae\u8bbe\u8ba1\u548c\u9a8c\u8bc1<\/h3>\n\n\n\n<p>MM<sub>\u8bd5\u5242\u76d2<\/sub>\u534f\u8bae\uff1a<a><\/a><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u51c6\u5907\u4e24\u4e2a\u6df1\u5b54\u677f\uff0c\u4e00\u4e2a\u6df1\u5b54\u677f\u6bcf\u5b54\u88c5\u6709 500\u03bcL MagMAX\u2122 CORE Wash Solution 1\uff0c\u53e6\u4e00\u4e2a\u6df1\u5b54\u677f\u6bcf\u5b54\u88c5\u6709 500\u03bcL MagMAX\u2122 CORE Wash Solution 2\u3002\u51c6\u5907\u4e00\u4e2a MagMAX\u2122 Express 96 \u5fae\u91cf\u6ef4\u5b9a\u677f\uff0c\u6bcf\u4e2a\u5b54\u88c5\u6709 90\u03bcL MagMAX\u2122 CORE Elution Buffer\u3002<\/li>\n\n\n\n<li>\u6bcf\u4e2a\u6837\u54c1\u51c6\u5907 20\u03bcL MagMAX\u2122 CORE \u78c1\u73e0\u548c 10\u03bcL MagMAX\u2122 CORE \u86cb\u767d\u9176 K \u7684\u6df7\u5408\u7269\uff08\u73e0\u5b50\/PK \u6df7\u5408\u7269\uff09\u3002<\/li>\n\n\n\n<li>\u4e3a\u6bcf\u4e2a\u6837\u54c1\u51c6\u5907 300\u03bcL MagMAX\u2122 CORE \u88c2\u89e3\u6eb6\u6db2\u548c 300\u03bcL MagMAX\u2122 CORE \u7ed3\u5408\u6eb6\u6db2\u7684\u6df7\u5408\u7269\uff08\u88c2\u89e3\/\u7ed3\u5408\u6eb6\u6db2\uff09\u3002<\/li>\n\n\n\n<li>\u5728\u6df1\u5b54\u677f\u4e2d\u6bcf\u5b54\u5206\u914d 30\u03bcL \u73e0\u5b50\/PK \u6df7\u5408\u7269\u3001700\u03bcL \u88c2\u89e3\/\u7ed3\u5408\u6eb6\u6db2\u548c 200\u03bcL \u6837\u54c1\u3002<\/li>\n\n\n\n<li>\u5c06\u6240\u6709\u677f\u653e\u5165\u7cfb\u7edf\u5e76\u8fd0\u884c\u811a\u672c MagMAX_CORE_KF-96\u3002<\/li>\n<\/ol>\n\n\n\n<p>MM<sub>\u5185\u90e8<\/sub>\u534f\u8bae\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u51c6\u5907\u4e24\u4e2a\u6df1\u5b54\u677f\uff0c\u6bcf\u4e2a\u5b54\u52a0\u5165 500\u03bcL 70% \u4e59\u9187\u3002\u51c6\u5907\u4e00\u4e2a MagMAX\u2122 Express 96 \u5fae\u91cf\u6ef4\u5b9a\u677f\uff0c\u6bcf\u4e2a\u5b54\u52a0\u5165 90\u03bcL \u6d17\u8131\u7f13\u51b2\u6db2\uff08Omega BIO-TEK\uff0c\u7f8e\u56fd\u4f50\u6cbb\u4e9a\u5dde\u8bfa\u514b\u7f57\u65af\uff09\u3002<\/li>\n\n\n\n<li>\u5728\u6df1\u5b54\u677f\u4e2d\u6bcf\u5b54\u5206\u914d 40 \u03bcL \u78c1\u73e0\uff08Mag-Bind\u00ae TotalPure NGS\uff0cOmega Bio-Tek\uff0c\u7f8e\u56fd\u4f50\u6cbb\u4e9a\u5dde\u8bfa\u514b\u7f57\u65af\uff09\u3001500 \u03bcL \u5f02\u4e19\u9187\u548c 500 \u03bcL \u6837\u54c1\u3002<\/li>\n\n\n\n<li>\u5c06\u6240\u6709\u677f\u653e\u5165\u7cfb\u7edf\u5e76\u8fd0\u884c\u811a\u672c MagMAX_CORE_KF-96\u3002<\/li>\n<\/ol>\n\n\n\n<p>OT-2<sub>\u5185\u90e8<\/sub>\u534f\u8bae\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5c06 40\u03bcL \u78c1\u73e0\uff08Mag-Bind\u00ae TotalPure NGS\uff0cOmega Bio-Tek\uff0c\u7f8e\u56fd\u4f50\u6cbb\u4e9a\u5dde\u8bfa\u514b\u7f57\u65af\uff09\u3001250\u03bcL \u5f02\u4e19\u9187\u548c 250\u03bcL \u6837\u54c1\u5206\u88c5\u5230\u6df1\u5b54\u677f\u4e2d\u3002\u7528\u79fb\u6db2\u5668\u5439\u6253\u4e94\u6b21\u6df7\u5300\uff0c\u5ba4\u6e29\u4e0b\u5b75\u80b2 5 \u5206\u949f\u3002<\/li>\n\n\n\n<li>\u6fc0\u6d3bGEN1\u78c1\u6027\u6a21\u57574\u5206\u949f\u3002<\/li>\n\n\n\n<li>\u6536\u96c6\u4e0a\u6e05\u6db2\u5e76\u5f03\u53bb\u3002<\/li>\n\n\n\n<li>\u52a0\u5165500\u03bcL 70%\u4e59\u9187\uff0c\u6536\u96c6\u5e76\u4e22\u5f03\u3002<\/li>\n\n\n\n<li>\u52a0\u5165500\u03bcL 70%\u4e59\u9187\uff0c\u6536\u96c6\u5e76\u4e22\u5f03\u3002<\/li>\n\n\n\n<li>\u98ce\u5e72 4 \u5206\u949f\u3002<\/li>\n\n\n\n<li>\u5173\u95edGEN1\u78c1\u6027\u6a21\u5757\u5e76\u6dfb\u52a0100\u03bcL\u6d17\u8131\u7f13\u51b2\u6db2\uff08Omega BIO-TEK\uff0c\u7f8e\u56fd\u4f50\u6cbb\u4e9a\u5dde\u8bfa\u514b\u7f57\u65af\uff09\u3002<\/li>\n\n\n\n<li>30\u79d2\u540e\u6253\u5f00GEN1\u78c1\u6027\u6a21\u5757\u3002<\/li>\n\n\n\n<li>90\u79d2\u540e\u6536\u96c6\u4e0a\u6e05\u6db2\u5e76\u8f6c\u79fb\u81f396\u5b54\u5fae\u91cf\u6ef4\u5b9a\u677f\u3002<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\">\u6837\u54c1\u8f93\u5165\u91cf\u662f\u81ea\u52a8\u79fb\u6db2\u7cfb\u7edf\u5141\u8bb8\u7684\u6700\u5927\u91cf\uff0c\u5176\u4ed6\u8bd5\u5242\u7684\u91cf\u4e5f\u4e0d\u540c\uff0c\u56e0\u6b64\u4e09\u79cd\u65b9\u6cd5\u7684\u8f93\u5165\u91cf\u4e0d\u540c<\/h3>\n\n\n\n<p>OT-2\u5185\u90e8\u534f\u8bae\u662f\u6839\u636e Opentrons \u8bf4\u660e\u7528 Python \u7f16\u5199\u7684\u3002\u811a\u672c\u5df2\u5b58\u653e\u5728 GitHub \u5b58\u50a8\u5e93\u4e2d<\/p>\n\n\n\n<p>\u4e3a\u4e86\u9a8c\u8bc1\u5185\u90e8\u6838\u9178\u63d0\u53d6\u65b9\u6848\u7684\u6027\u80fd\uff0c\u4f7f\u7528 DNA \u9633\u6027\u5bf9\u7167 TaqMan 2019-nCoV \u5bf9\u7167\u8bd5\u5242\u76d2 v1\uff08\u8d5b\u9ed8\u98de\u4e16\u5c14\u79d1\u6280\uff0c\u7f8e\u56fd\u9a6c\u8428\u8bf8\u585e\u5dde\u6c83\u5c14\u745f\u59c6\uff09\u5236\u4f5c\u4e86\u6807\u51c6\u3001\u4f4e\u548c\u6781\u4f4e\u75c5\u6bd2\u8f7d\u91cf\u7684\u6a21\u62df\u6837\u672c\u3002\u5bf9\u7167\u8bd5\u5242\u76d2\u7684\u6d53\u5ea6\u4e3a 1 x 10&nbsp;<sup>4<\/sup>\u62f7\u8d1d\/\u03bcL\u3002\u6807\u51c6\u75c5\u6bd2\u8f7d\u91cf\u6837\u672c\u7684\u5236\u5907\u65b9\u6cd5\u662f\u5c06 10 \u03bcL \u9633\u6027\u5bf9\u7167\u4e0e 490 \u03bcL \u75c5\u6bd2\u8fd0\u8f93\u57f9\u517b\u57fa\u548c 500 \u03bcL GTC \u6df7\u5408\u3002\u4f4e\u548c\u6781\u4f4e\u75c5\u6bd2\u8f7d\u91cf\u7684\u5236\u5907\u65b9\u5f0f\u76f8\u540c\uff0c\u4f46\u4f7f\u7528\u9633\u6027\u5bf9\u7167\u7684\u5341\u500d\u8fde\u7eed\u7a00\u91ca\u6db2\u3002\u6240\u6709\u6a21\u62df\u6837\u672c\u5747\u5236\u5907\u4e09\u4efd\uff0c\u5e76\u4e0e<sub>\u5185\u90e8<\/sub>OT-2 \u3001MM<sub>\u8bd5\u5242\u76d2\u548c<\/sub><sub>\u5185\u90e8<\/sub>MM\u5e76\u884c\u5904\u7406\uff0c\u7136\u540e\u4f7f\u7528 TaqMan 2019-nCoV \u68c0\u6d4b\u8bd5\u5242\u76d2 v1 \u8fdb\u884c qPCR \u6d4b\u8bd5\u3002\u6807\u51c6\u3001\u4f4e\u548c\u6781\u4f4e\u75c5\u6bd2\u8f7d\u91cf\u7684\u6a21\u62df\u6837\u672c\u4e2d\u9633\u6027\u5bf9\u7167\u7684\u6700\u7ec8\u6d53\u5ea6\u5206\u522b\u4e3a 1 x 10&nbsp;<sup>6<\/sup>\u62f7\u8d1d\/mL\u30011 x 10&nbsp;<sup>5<\/sup>\u62f7\u8d1d\/mL \u548c 1 x 10&nbsp;<sup>4<\/sup>\u62f7\u8d1d\/mL\u3002\u6240\u6709\u8fd0\u884c\u5747\u5305\u62ec\u9634\u6027\u5bf9\u7167\u3002<a><\/a><\/p>\n\n\n\n<p>\u901a\u8fc7\u5c06\u6a21\u62df\u6837\u672c\u4e2d\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c (Ct&nbsp;<sub>ss<\/sub>&nbsp;) \u4e0e\u76f4\u63a5\u4ece\u5e93\u5b58\u5236\u5907\u7684\u9633\u6027\u5bf9\u7167\u7684 Ct \u503c\u8fdb\u884c\u6bd4\u8f83\u6765\u8ba1\u7b97\u6838\u9178\u63d0\u53d6\u6548\u7387\uff0c\u6821\u6b63\u91cf\u4ee5\u5339\u914d\u7a00\u91ca\u56e0\u5b50\u548c\u6bcf\u4e2a\u65b9\u6848\u4e2d\u4f7f\u7528\u7684\u521d\u59cb\u6837\u672c\u91cf (Ct&nbsp;<sub>pc<\/sub>&nbsp;) (R = 2&nbsp;<sup>-\u0394Ct<\/sup>&nbsp;= 2&nbsp;<sup>-(Ctss-Ctpc)<\/sup>&nbsp;)\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u5b9a\u91cfPCR<\/h3>\n\n\n\n<p>\u4f7f\u7528\u9488\u5bf9<em>orf1ab<\/em>\u3001\u523a\u7a81 (S)\u3001\u6838\u8863\u58f3 (N) \u548c\u4eba RNaseP \u57fa\u56e0\u7684 TaqMan 2019-nCoV \u68c0\u6d4b\u8bd5\u5242\u76d2 v1 \u4ee5\u53ca\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\u7684 TaqMan 2019-nCoV \u5bf9\u7167\u8bd5\u5242\u76d2 v1\uff0c\u6309\u7167\u5236\u9020\u5546\u63a8\u8350\u7684 qPCR \u6761\u4ef6\u5bf9 SARS-CoV-2 \u75c5\u6bd2 RNA \u8fdb\u884c\u6838\u9178\u6269\u589e\u3002\u6240\u6709 qPCR \u68c0\u6d4b\u5747\u5728 CFX96 Touch \u5b9e\u65f6 PCR \u68c0\u6d4b\u7cfb\u7edf (Bio-Rad\uff0c\u7f8e\u56fd\u52a0\u5229\u798f\u5c3c\u4e9a\u5dde\u8d6b\u62c9\u514b\u52d2\u65af) \u4e2d\u8fdb\u884c\u3002\u4e3a\u4e86\u51cf\u5c11\u68c0\u6d4b\u95f4\u5dee\u5f02\uff0c\u63d0\u53d6\u7684\u6838\u9178\u6837\u672c\u4f7f\u7528\u53e6\u4e00\u4e2a OT-2 \u6a21\u5757\u81ea\u52a8\u5206\u914d\u5230 qPCR \u8bd5\u7eb8\u4e2d\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u7edf\u8ba1\u5206\u6790<\/h3>\n\n\n\n<p>\u6210\u5bf9\u6bd4\u8f83\u4e86\u8fd9\u4e09\u79cd\u65b9\u6848\u3002\u4f7f\u7528 McNemar \u68c0\u9a8c\u6765\u6bd4\u8f83\u5b83\u4eec\u5c06\u6837\u672c\u5206\u914d\u4e3a\u9633\u6027\u6216\u9634\u6027\u7684\u8868\u73b0\u3002Ct \u503c\u4e0d\u5448\u6b63\u6001\u5206\u5e03\uff0c\u56e0\u6b64\u4f7f\u7528 Wilcoxon \u7b26\u53f7\u79e9\u68c0\u9a8c\u6765\u6bd4\u8f83\u6bcf\u4e2a\u76ee\u6807\u7684 Ct \u503c\u3002\u5bf9\u4e8e\u6bcf\u4e2a\u76ee\u6807\uff0c\u4ec5\u8003\u8651\u901a\u8fc7\u8fd9\u4e09\u79cd\u65b9\u6cd5\u6269\u589e\u7684\u6837\u672c\u3002\u4f7f\u7528 bootstrap \u65b9\u6cd5\u8ba1\u7b97\u4e2d\u4f4d\u7f6e\u4fe1\u533a\u95f4\u3002<a><\/a><\/p>\n\n\n\n<p>\u6240\u6709\u7edf\u8ba1\u6d4b\u8bd5\u5747\u91c7\u7528 IBM SPSS Statistics 24.0.0.0 \u8f6f\u4ef6\u5305\uff08SPSS Inc.\uff0c\u7f8e\u56fd\u4f0a\u5229\u8bfa\u4f0a\u5dde\u829d\u52a0\u54e5\uff09\u6267\u884c\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u7ed3\u679c<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">\u63d0\u53d6\u65b9\u6848\u7684\u6548\u7387<\/h3>\n\n\n\n<p>\u901a\u8fc7\u63d0\u53d6\u7528\u6a21\u62df\u4e0d\u540c\u75c5\u6bd2\u8f7d\u91cf\u7684 SARS-CoV-2 \u9633\u6027\u5bf9\u7167\u5236\u5907\u7684\u6a21\u62df\u6837\u672c\uff0c\u5c06\u4e24\u79cd\u5185\u90e8\u65b9\u6848\u7684\u6838\u9178\u63d0\u53d6\u6548\u7387\u4e0e MM<sub>\u8bd5\u5242\u76d2\u65b9\u6848\u8fdb\u884c\u4e86\u6bd4\u8f83\u3002MM<\/sub><sub>\u8bd5\u5242\u76d2<\/sub>\u548c OT-2<sub>\u5185\u90e8<\/sub>\u65b9\u6848\u6210\u529f\u6269\u589e\u4e86\u6240\u6709\u5177\u6709\u6807\u51c6\u75c5\u6bd2\u8f7d\u91cf\u7684\u6a21\u62df\u6837\u672c\u4e2d\u7684<em>orf1ab<\/em>\u3001S \u548c N \u9776\u6807\u3002\u5bf9\u4e8e\u8fd9\u4e9b\u6837\u672c\uff0c\u6240\u6709\u91cd\u590d\u548c\u57fa\u56e0\u7684\u5e73\u5747\u63d0\u53d6\u6548\u7387\u4e3a MM<sub>\u8bd5\u5242\u76d233.9%\uff08SD\uff1a13.8\uff09\uff0cOT-2<\/sub><sub>\u5185\u90e8<\/sub>\u65b9\u684819.6%\uff08SD\uff1a2.2\uff09\uff0cMM<sub>\u5185\u90e8\u65b9\u6848<\/sub>16.0%\uff08SD\uff1a5.03\uff09 \u3002<a><\/a><\/p>\n\n\n\n<p>\u5728\u4f4e\u75c5\u6bd2\u8f7d\u91cf\u6a21\u62df\u6837\u672c\u4e2d\uff0cMM<sub>\u8bd5\u5242\u76d2<\/sub>\u65e0\u6cd5\u6269\u589e\u6240\u6709\u6837\u672c\u4e2d\u7684 S \u9776\u6807\uff0c\u4e5f\u65e0\u6cd5\u6269\u589e\u5176\u4e2d\u4e00\u4e2a\u91cd\u590d\u6837\u672c\u4e2d\u7684 N \u9776\u6807\uff0c\u800c OT-2<sub>\u5185\u90e8<\/sub>\u548c MM<sub>\u5185\u90e8<\/sub>\u68c0\u6d4b\u51fa\u4e86\u6240\u6709\u57fa\u56e0\u3002\u6700\u540e\uff0cMM<sub>\u8bd5\u5242\u76d2<\/sub>\u548c OT-2<sub>\u5185\u90e8<\/sub>\u65b9\u6848\u65e0\u6cd5\u6269\u589e\u6781\u4f4e\u75c5\u6bd2\u8f7d\u91cf\u6837\u672c\uff0c\u9664\u4e86\u4e00\u4e2a\u91cd\u590d\u6837\u672c\uff0c\u5176\u4e2d N \u57fa\u56e0\u53ef\u4ee5\u901a\u8fc7 OT-2<sub>\u5185\u90e8<\/sub>\u65b9\u6848\u6269\u589e\uff0c\u4f46 MM<sub>\u5185\u90e8<\/sub>\u53ef\u4ee5\u68c0\u6d4b\u5230\u6240\u6709\u6837\u672c\u4e2d\u7684\u9633\u6027\u5bf9\u7167\uff0c\u5176\u4e2d\u4e00\u4e2a\u6837\u672c\u542b\u6709\u4e09\u4e2a\u9776\u6807\uff0c\u4e00\u4e2a\u6837\u672c\u542b\u6709 orf1ab<em>\u548c<\/em>N \u9776\u6807\uff0c\u6700\u540e\u4e00\u4e2a\u6837\u672c\u542b\u6709<em>orf1ab<\/em>\u9776\u6807\uff08S1 \u8868\uff09\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u4e34\u5e8a\u547c\u5438\u9053\u6837\u672c\u7684\u6838\u9178\u63d0\u53d6<\/h3>\n\n\n\n<p>\u6536\u96c6\u7684 141 \u4efd\u9633\u6027\u4e34\u5e8a\u6837\u672c\u540c\u65f6\u4f7f\u7528 MM<sub>\u8bd5\u5242\u76d2<\/sub>\u3001OT-2<sub>\u5185\u90e8<\/sub>\u65b9\u6cd5\u548c MM<sub>\u5185\u90e8<\/sub>\u65b9\u6cd5\u8fdb\u884c\u6838\u9178\u63d0\u53d6\uff0c\u5e76\u901a\u8fc7 qPCR \u68c0\u6d4b\u6d17\u8131\u7684 SARS-CoV-2 RNA\u3002\u8bb0\u5f55\u6bcf\u4e2a\u76ee\u6807\u7684\u9633\u6027\/\u9634\u6027\u7ed3\u679c\u548c\u6269\u589e\u5faa\u73af\u9608\u503c (Ct)\u3002\u6839\u636e\u5236\u9020\u5546\u7684\u8bf4\u660e\uff0c\u5f53\u4e09\u4e2a\u76ee\u6807\u533a\u57df\u4e2d\u81f3\u5c11\u4e00\u4e2a\u6269\u589e\u4e14 Ct&lt;40 \u65f6\uff0c\u6837\u672c\u88ab\u89c6\u4e3a\u9633\u6027\u3002\u6240\u6709\u8fd0\u884c\u4e2d\u5305\u62ec\u7684\u9634\u6027\u5bf9\u7167\u5728\u6240\u6709\u60c5\u51b5\u4e0b\u5747\u4ea7\u751f\u9634\u6027 PCR \u7ed3\u679c\u3002<a><\/a><\/p>\n\n\n\n<p>141 \u4e2a\u6837\u672c\u4e2d\uff0c123 \u4e2a\u6837\u672c\u901a\u8fc7\u81f3\u5c11\u4e00\u79cd\u63d0\u53d6\u65b9\u6cd5\u68c0\u6d4b\u51fa SARS-CoV-2 \u9633\u6027\uff0c\u800c 18 \u4e2a\u6837\u672c\u901a\u8fc7\u4e09\u79cd\u65b9\u6cd5\u68c0\u6d4b\u5747\u4e3a\u9634\u6027\u3002\u8fd9\u53ef\u80fd\u662f\u7531\u4e8e\u6837\u672c\u5728\u91c7\u96c6\u671f\u95f4\u964d\u89e3\u6240\u81f4\uff0c\u56e0\u4e3a\u5b83\u4eec\u5df2\u5728 4\u00b0C \u4e0b\u50a8\u5b58\u4e86\u7ea6 48 \u5c0f\u65f6 [9]\u3002\u6309\u65b9\u6cd5\u5206\u7c7b\uff0c114 \u4e2a\u6837\u672c\u4f7f\u7528 MM<sub>\u8bd5\u5242\u76d2\u68c0\u6d4b\u5448\u9633\u6027\uff0c111 \u4e2a\u6837\u672c\u4f7f\u7528\u5185\u90e8<\/sub>OT-2 \u68c0\u6d4b\u5448\u9633\u6027\uff0c118 \u4e2a\u6837\u672c\u4f7f\u7528<sub>\u5185\u90e8<\/sub>MM \u68c0\u6d4b\u5448\u9633\u6027\u3002\u6210\u5bf9\u6bd4\u8f83\u53d1\u73b0\u5b83\u4eec\u68c0\u6d4b SARS-CoV-2 \u7684\u6027\u80fd\u6ca1\u6709\u663e\u8457\u5dee\u5f02\uff08MM<sub>\u8bd5\u5242\u76d2<\/sub>Vs OT-2<sub>\u5185\u90e8<\/sub>\uff0c<em>P =<\/em>\u00a00.5465\uff1bMM<sub>\u8bd5\u5242\u76d2<\/sub>Vs MM<sub>\u5185\u90e8<\/sub>\uff0c<em>P =<\/em>\u00a00.3865\uff1bOT-2<sub>\u5185\u90e8<\/sub>Vs MM<sub>\u5185\u90e8<\/sub>\uff0c<em>P =<\/em>\u00a00.0961\uff09\u300218 \u4e2a\u6837\u672c\u901a\u8fc7\u4e09\u79cd\u65b9\u6cd5\u4e2d\u7684\u4e00\u4e9b\u65b9\u6cd5\u68c0\u6d4b\u5448\u9634\u6027\u3002\u8fd9\u4e9b\u5177\u6709\u8f83\u9ad8\u7684 Ct \u503c\uff0c\u00a0<em>orf1ab<\/em>\u548c N \u9776\u6807\u7684\u4e2d\u503c\u5206\u522b\u4e3a 38.68 \u548c 38.19\uff08\u5728\u8fd9\u4e9b\u6837\u672c\u4e2d\uff0cS \u9776\u6807\u672a\u901a\u8fc7\u4efb\u4f55\u65b9\u6cd5\u6269\u589e\uff09\u3002\u6bd4\u8f83\u6240\u6709\u65b9\u6cd5\u548c\u9776\u6807\u7684\u6210\u5bf9\u7ebf\u6027\u56de\u5f52\u548c\u76f8\u5173\u6027\u5206\u6790\u663e\u793a R\u00a0<sup>2<\/sup>\u503c\u5728 0.85 \u548c 0.95 \u4e4b\u95f4\uff0cBland-Altman \u5206\u6790\u8868\u660e\u5728\u6574\u4e2a Ct \u8303\u56f4\u5185\u4e00\u81f4\u6027\u662f\u4e00\u81f4\u7684\uff08S1 \u56fe\uff09\u3002<\/p>\n\n\n\n<p><sub>\u5728\u4f7f\u7528 MM\u8bd5\u5242\u76d2<\/sub>\u63d0\u53d6\u7684\u6837\u672c\u4e2d\uff0c43% \u53ef\u68c0\u6d4b\u5230\u4e09\u4e2a\u9776\u6807\uff08<em>orf1ab \u3001 N \u548c S \uff09\uff0c\u5728\u4f7f\u7528<\/em><sub>\u5185\u90e8<\/sub>OT-2 \u63d0\u53d6\u7684\u6837\u672c\u4e2d\uff0c49%\u53ef\u68c0\u6d4b\u5230\uff0c<sub>\u5728\u4f7f\u7528\u5185\u90e8<\/sub>MM \u63d0\u53d6\u7684\u6837\u672c\u4e2d\uff0c49% \u53ef\u68c0\u6d4b\u5230\uff08\u56fe 1\uff09\u3002\u5728 34%\u300139% \u548c 30% \u7684\u6837\u672c\u4e2d\u68c0\u6d4b\u5230\u4e86<em>orf1ab<\/em>\u548c N \u9776\u6807\uff0c\u5728 19%\u300110% \u548c 17% \u7684\u6837\u672c\u4e2d\u4ec5\u6269\u589e\u4e86 N \u9776\u6807\u3002\u53ea\u6709\u6781\u5c11\u6570\u6837\u672c\u56e0\u5355\u72ec\u6269\u589e<em>orf1ab<\/em>\u9776\u6807\uff086\uff09\u3001<em>orf1ab<\/em>\u00a0+ S\uff081\uff09\u6216 N + S\uff085\uff09\u9776\u6807\u800c\u88ab\u89c6\u4e3a\u9633\u6027\uff0c\u5e76\u4e14\u6ca1\u6709\u6837\u672c\u4ee5 S \u57fa\u56e0\u4f5c\u4e3a\u552f\u4e00\u7684\u9633\u6027\u6807\u8bb0\u3002\u4e8b\u5b9e\u4e0a\uff0c\u8be5\u68c0\u6d4b\u4e2d\u7684 S \u9776\u6807\u660e\u663e\u7f3a\u4e4f\u7075\u654f\u5ea6\uff0c\u5e76\u4e14\u4e0e\u8bca\u65ad\u51b3\u7b56\u65e0\u5173\u3002\u4f7f\u7528 OT-2<sub>\u5185\u90e8<\/sub>\u534f\u8bae\u68c0\u6d4b\u5230\u4e24\u4e2a\u6216\u4e09\u4e2a\u76ee\u6807\u7684\u6837\u672c\u5360 87%\uff0897 \u4e2a\u6837\u672c\uff09\uff0c\u4f7f\u7528 MM<sub>\u5185\u90e8\u534f\u8bae\u68c0\u6d4b\u5230\u4e24\u4e2a\u6216\u4e09\u4e2a\u76ee\u6807\u7684\u6837\u672c\u5360 82%\uff0895 \u4e2a\u6837\u672c\uff09\uff0c\u4f7f\u7528 MM\u8bd5\u5242\u76d2<\/sub>\u68c0\u6d4b\u5230\u4e24\u4e2a\u6216\u4e09\u4e2a\u76ee\u6807\u7684\u6837\u672c\u5360 79%\u00a0\uff0890 \u4e2a\u6837\u672c\uff09\u3002<a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0246302#pone-0246302-g001\"><\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"518\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-1024x518.png\" alt=\"\u6837\u672c\u7684\u767e\u5206\u6bd4\" class=\"wp-image-3122\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-1024x518.png 1024w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-300x152.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-768x389.png 768w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-1536x778.png 1536w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-2048x1037.png 2048w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-8-576x292.png 576w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">\u56fe 1.\u4f7f\u7528 MM\u8bd5\u5242\u76d2\u3001OT-2\u5185\u90e8\u65b9\u6cd5\u548c MM\u5185\u90e8\u65b9\u6cd5\u5bf9orf1ab\u3001S \u548c N \u9776\u6807\u5448\u9633\u6027\u7684 SARS-CoV-2 \u6837\u672c\u7684\u767e\u5206\u6bd4\u3002\u6761\u5f62\u56fe\u4e0a\u7684\u6570\u5b57\u8868\u793a\u6bcf\u4e2a\u76ee\u6807\u7684\u9633\u6027\u6837\u672c\u767e\u5206\u6bd4\u3002\u603b\u4f53\u800c\u8a00\uff0c114 \u4e2a (81%) \u6837\u672c\u4f7f\u7528 MM\u8bd5\u5242\u76d2\u68c0\u6d4b\u5448\u9633\u6027\uff0c111 \u4e2a (79%) \u4f7f\u7528\u5185\u90e8OT-2 \u68c0\u6d4b\u5448\u9633\u6027\uff0c118 \u4e2a (84%) \u4f7f\u7528\u5185\u90e8MM \u68c0\u6d4b\u5448\u9633\u6027\u3002<\/figcaption><\/figure>\n\n\n\n<p>\u8003\u8651\u914d\u5bf9\u6837\u672c\u65f6\uff0c\u5728 orf1ab ( P = 0.437)\u3001N ( P = 0.686) \u548c S ( P = 0.794) \u9776\u6807\u7684 Ct \u503c\u4e2d\uff0cMM \u8bd5\u5242\u76d2\u548c OT-2 \u5185\u90e8\u65b9\u6848\u4e4b\u95f4\u7684 Cts \u6ca1\u6709\u663e\u8457\u5dee\u5f02<em>( \u56fe 2 )\u3002\u4e0e MM \u8bd5\u5242\u76d2 ( orf1ab<\/em>\u00a0;\u00a0<em>P<\/em>\u00a0&lt;\u00a0<em>0.00001<\/em>\u00a0,\u00a0<em>N<\/em>\u00a0;\u00a0<sub>P<\/sub>\u00a0&lt;\u00a0<sub>0.00001<\/sub>\u00a0,\u00a0S\u00a0;\u00a0P\u00a0=\u00a00.00148\u00a0<em>)<\/em>\u548c<em>OT<\/em>\u00a0&#8211;\u00a0<em>2<\/em>\u5185\u90e8<sub>\u65b9\u6848(<\/sub>\u00a0orf1ab\u00a0<em>;<\/em>\u00a0P\u00a0<em>&lt;<\/em>\u00a00.00001, N;\u00a0<em>P =<\/em>\u00a00.00008, S;\u00a0<em>P =<\/em>\u00a00.00252)\u76f8\u6bd4\uff0cMM<sub>\u5185\u90e8\u65b9\u6848\u5728\u6240\u6709\u9776\u6807\u4e2d\u7684 Cts \u786e\u5b9e\u663e<\/sub><em>\u8457\u8f83\u4f4e\u3002<\/em><a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0246302#pone-0246302-g002\"><\/a><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/journals.plos.org\/plosone\/article\/figure\/image?size=medium&amp;id=10.1371\/journal.pone.0246302.g002\"><img decoding=\"async\" src=\"https:\/\/journals.plos.org\/plosone\/article\/figure\/image?size=inline&amp;id=10.1371\/journal.pone.0246302.g002\" alt=\"\u7f29\u7565\u56fe\"\/><\/a><\/figure>\n\n\n\n<p>\u56fe 2.\u7bb1\u7ebf\u56fe\u663e\u793a\u4f7f\u7528 MM<sub>\u8bd5\u5242\u76d2<\/sub>\u3001OT-2<sub>\u5185\u90e8<\/sub>\u65b9\u6cd5\u548c MM<sub>\u5185\u90e8\u65b9\u6cd5\u83b7\u5f97\u7684<\/sub><em>orf1ab<\/em>\u00a0\u3001S \u548c N \u76ee\u6807\u7684 Ct \u503c\u5206\u5e03\u3002y \u8f74\u663e\u793a\u6269\u589e\u5faa\u73af (Ct)\u3002\u663e\u793a\u6bcf\u4e2a\u6570\u636e\u96c6\u4e0a\u7684\u6570\u636e\u70b9\u6570\u91cf\u3002<\/p>\n\n\n\n<p>\u4f7f\u7528 MM<sub>\u8bd5\u5242\u76d2<\/sub>\u3001OT-2<sub>\u5185\u90e8\u8bd5\u5242\u76d2<\/sub>\u548c MM<sub>\u5185\u90e8\u8bd5\u5242\u76d2\u5bf9<\/sub><em>orf1ab<\/em>\u9776\u6807\u7684\u4e2d\u4f4d\u6269\u589e\u5faa\u73af (CI:95%)\u5206\u522b\u4e3a 35.53 (33.82\u201336.36)\u300135.58 (34.33\u201336.21) \u548c 34.8 (33.71\u201335.29)\u3002\u5bf9\u4e8e S \u57fa\u56e0\uff0c\u4e2d\u4f4d\u6269\u589e\u5faa\u73af (CI:95%) \u4e3a 30.16 (28.56\u201333.08)\u300131.32 (29.22\u201332.88) \u548c 31.07 (29.36\u201332.90)\uff1b\u5bf9\u4e8e N \u9776\u6807\uff0c\u4e2d\u4f4d\u6269\u589e\u5faa\u73af (CI:95%) \u4e3a 34.83 (33.93\u201335.97)\u300134.64 (33.42\u201335.29) \u548c 34.28 (33.52\u201335.10)\u3002<sub><\/sub><sub><\/sub><sub><\/sub><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u5f00\u91c7\u6210\u672c\u548c\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4<\/h3>\n\n\n\n<p><sub>\u4f7f\u7528 OT-2\u5185\u90e8<\/sub>\u534f\u8bae\u63d0\u53d6 96 \u4e2a\u6837\u672c\u7684\u6838\u9178\uff0c\u603b\u6210\u672c\u4e3a 107 \u6b27\u5143\uff08\u8bd5\u5242 37 \u6b27\u5143\u548c\u5b9e\u9a8c\u5ba4\u5668\u5177 70 \u6b27\u5143\uff09\uff0c\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u4e3a 10 \u5206\u949f\uff0c\u63d0\u53d6\u65f6\u95f4\u4e3a 3 \u4e2a\u534a\u5c0f\u65f6\uff08\u811a\u672c\u6bcf\u6b21\u8fd0\u884c\u5904\u7406 48 \u4e2a\u6837\u672c\uff0c\u56e0\u6b64\u5fc5\u987b\u5b8c\u6210\u4e24\u6b21\uff09\u3002MM<sub>\u5185\u90e8<\/sub>\u6210\u672c\u4e3a 66 \u6b27\u5143\uff08\u8bd5\u5242 37 \u6b27\u5143\u548c\u5b9e\u9a8c\u5ba4\u5668\u5177 29 \u6b27\u5143\uff09\uff0cMM \u8bd5\u5242<sub>\u76d2<\/sub>\u6210\u672c\u4e3a 472 \u6b27\u5143\uff08\u8bd5\u5242 443 \u6b27\u5143\u548c\u5b9e\u9a8c\u5ba4\u5668\u5177 29 \u6b27\u5143\uff09\u3002\u4e24\u79cd\u534f\u8bae\u7684\u5b9e\u9645\u64cd\u4f5c\u65f6\u95f4\u5747\u4e3a 40 \u5206\u949f\uff0c\u63d0\u53d6\u65f6\u95f4\u5747\u4e3a 30 \u5206\u949f\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u867d\u7136 96 \u4e2a\u6837\u672c\u7684 OT-2 \u534f\u8bae\u6bd4 MM<sub>\u5185\u90e8<\/sub>\u534f\u8bae\u8d35 40 \u6b27\u5143\uff0c\u4f46\u6240\u9700\u7684\u521d\u59cb\u6295\u8d44\u660e\u663e\u8f83\u4f4e\uff0cOT-2 \u7cfb\u7edf\uff08\u5305\u62ec\u6a21\u5757\u548c\u79fb\u6db2\u5668\uff09\u7ea6\u4e3a 10,000 \u6b27\u5143\uff0cMagMAX&nbsp;<sup>TM<\/sup>\u7cfb\u7edf\u7ea6\u4e3a 50,000 \u6b27\u5143\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u8ba8\u8bba<\/h2>\n\n\n\n<p>\u672c\u6587\u4ecb\u7ecd\u4e86\u4e24\u79cd\u4f4e\u6210\u672c\u75c5\u6bd2 RNA \u63d0\u53d6\u548c\u4e34\u5e8a\u6837\u672c SARS-CoV-2 \u68c0\u6d4b\u65b9\u6cd5\uff0c\u5176\u6027\u80fd\u4e0e\u5546\u7528\u8bd5\u5242\u76d2\u76f8\u5f53\u3002\u5c3d\u7ba1\u5185\u90e8\u65b9\u6848\u63d0\u53d6 DNA \u5bf9\u7167\u7684\u6548\u7387\u4f4e\u4e8e\u5546\u7528\u8bd5\u5242\u76d2\uff0c\u4f46\u5728\u4e34\u5e8a\u6837\u672c\u4e2d\uff0c\u6574\u4f53\u7075\u654f\u5ea6\u5e76\u672a\u53d7\u5230\u5f71\u54cd\uff0c\u8fd9\u53ef\u80fd\u662f\u56e0\u4e3a\u5185\u90e8\u65b9\u6848\u4f7f\u7528\u66f4\u5927\u7684\u6837\u672c\u8d77\u59cb\u91cf\u6765\u5f25\u8865\u8f83\u4f4e\u7684\u6548\u7387\u3002<a><\/a><\/p>\n\n\n\n<p>\u8bbe\u7f6e OT-2 \u7cfb\u7edf\u8fdb\u884c RNA \u63d0\u53d6\u9700\u8981\u5bf9\u6ca1\u6709\u7ecf\u9a8c\u7684\u56e2\u961f\u8fdb\u884c\u5bc6\u96c6\u7f16\u7a0b\uff0c\u4f46\u63d0\u4f9b\u4e86\u4e00\u79cd\u7ecf\u6d4e\u9ad8\u6548\u7684\u66ff\u4ee3\u65b9\u6848\uff0c\u80fd\u591f\u5728\u4e00\u6b21\u8fd0\u884c\u4e2d\u63d0\u53d6 48 \u4e2a\u6837\u672c\u3002\u8fd9\u9879\u5de5\u4f5c\u4e2d\u4f7f\u7528\u7684\u811a\u672c\u5df2\u4e0a\u4f20\u5230\u5f00\u653e\u8bbf\u95ee\u8f6f\u4ef6\u5b58\u50a8\u5e93 GitHub\uff0c\u5728\u90a3\u91cc\u53ef\u4ee5\u627e\u5230\u8bb8\u591a\u7528\u4e8e\u8fd9\u4e9b\u548c\u7c7b\u4f3c\u5e94\u7528\u7a0b\u5e8f\u7684\u534f\u8bae\u3002\u8fd9\u5e94\u8be5\u6709\u52a9\u4e8e\u5176\u4ed6\u5de5\u4f5c\u4eba\u5458\u907f\u514d\u6216\u51cf\u5c11\u7f16\u7a0b\u6b65\u9aa4\u3002\u8be5\u534f\u8bae\u51e0\u4e4e\u4e0d\u9700\u8981\u52a8\u624b\u65f6\u95f4\uff0c\u5e76\u4e14\u4f7f\u7528\u5bb9\u6613\u83b7\u5f97\u7684\u8bd5\u5242\u3002\u6b64\u5916\uff0c\u4e0e\u5176\u4ed6\u63d0\u53d6\u7cfb\u7edf\u76f8\u6bd4\uff0c\u8be5\u8bbe\u5907\u6240\u9700\u7684\u6295\u8d44\u8f83\u4f4e\uff0c\u4f7f\u5176\u9002\u7528\u4e8e\u4e2d\u4f4e\u8d44\u6e90\u8bbe\u65bd\u3002MagMAX\u00a0<sup>TM<\/sup>\u00a0Express-96 \u662f\u4e00\u79cd\u5feb\u901f\u3001\u534a\u81ea\u52a8\u8bbe\u5907\uff0c\u6bcf\u6b21\u8fd0\u884c\u80fd\u591f\u63d0\u53d6 96 \u4e2a\u6837\u672c\u3002MM<sub>\u8bd5\u5242\u76d2<\/sub>\u63d0\u4f9b\u7528\u4e8e\u706d\u6d3b\u3001\u88c2\u89e3\u3001\u6d17\u6da4\u548c\u6d17\u8131\u7684\u8bd5\u5242\uff0c\u53ef\u4ee5\u4ee5\u5177\u6709\u7ade\u4e89\u529b\u7684\u6210\u672c\u7528\u4e8e\u4e0d\u540c\u7684\u57fa\u8d28\u548c\u6837\u54c1\u7c7b\u578b\u3002\u53e6\u4e00\u65b9\u9762\uff0cMM<sub>\u5185\u90e8<\/sub>\u534f\u8bae\u5229\u7528\u8be5\u7cfb\u7edf\u7684\u534a\u5f00\u653e\u65b9\u6cd5\uff0c\u7528\u5176\u4ed6\u5316\u5b66\u54c1\u66ff\u4ee3\u5546\u4e1a\u89e3\u51b3\u65b9\u6848\uff0c\u4f7f\u5176\u66f4\u5177\u6210\u672c\u6548\u76ca\u3002\u5728\u8fd9\u4e24\u79cd\u60c5\u51b5\u4e0b\uff0c\u90fd\u5fc5\u987b\u624b\u52a8\u51c6\u5907\u6df1\u5b54\u677f\u3001\u7f13\u51b2\u6db2\u548c\u6837\u54c1\uff0c\u8fd9\u4f1a\u589e\u52a0\u603b\u63d0\u53d6\u65f6\u95f4\u548c\u64cd\u4f5c\u9519\u8bef\u98ce\u9669\u3002\u8fd9\u4e24\u79cd\u5185\u90e8\u534f\u8bae\u7684\u4e3b\u8981\u7f3a\u70b9\u662f\uff0c\u5f53\u5904\u7406\u7c98\u7a20\u6837\u54c1\u65f6\uff0c\u7c98\u6db2\u3001\u9ad8\u7c98\u6027\u591a\u7cd6\u3001\u767d\u7ec6\u80de\u3001\u7ea2\u7ec6\u80de\u3001\u8840\u7ea2\u86cb\u767d\u3001\u86cb\u767d\u9176\u548c\u542b\u6709\u5927\u91cf\u7ec6\u80de\u6838\u9178\u7684\u7ec6\u80de\u788e\u5c51\u4f1a\u963b\u788d RNA \u63d0\u53d6\u6216\u6291\u5236 PCR \u53cd\u5e94 [\u00a010-12\u00a0]\u00a0\u3002\u4e3a\u4e86\u90e8\u5206\u514b\u670d\u8fd9\u4e2a\u95ee\u9898\uff0c\u53ef\u4ee5\u5728\u63d0\u53d6\u524d\u5bf9\u6837\u54c1\u8fdb\u884c\u52a0\u70ed\u548c\u6da1\u65cb\u6216\u79bb\u5fc3\uff0c\u4f46\u8fd9\u4f1a\u589e\u52a0\u52a8\u624b\u65f6\u95f4\u548c\u54cd\u5e94\u65f6\u95f4\u3002\u5f53\u4f7f\u7528<sub>\u5185\u90e8<\/sub>MM\u65f6\uff0c\u6d17\u8131\u6837\u54c1\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\u53ef\u80fd\u542b\u6709\u75d5\u91cf\u7684\u78c1\u73e0\uff0c\u4f46\u8fd9\u4e0d\u4f1a\u5f71\u54cd RT-PCR \u53cd\u5e94\u7684\u6027\u80fd\u3002<a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0246302#pone.0246302.ref012\"><\/a><\/p>\n\n\n\n<p>\u5728\u8fd9\u4e09\u4e2a\u7cfb\u7edf\u4e2d\uff0c\u9634\u6027\u5bf9\u7167\u5728\u6240\u6709\u60c5\u51b5\u4e0b\u5747\u4e3a\u9634\u6027\uff0c\u8868\u660e\u5728\u8fd9\u4e9b\u7cfb\u7edf\u4e2d\u5904\u7406\u539f\u59cb\u6837\u672c\u65f6\u4e0d\u5b58\u5728\u4ea4\u53c9\u6c61\u67d3\u95ee\u9898\u3002\u4e0d\u8fc7\uff0c\u4e0e\u975e\u81ea\u52a8\u5316\u7cfb\u7edf\u4e00\u6837\uff0c\u5e94\u91c7\u53d6\u9884\u9632\u63aa\u65bd\uff0c\u5c06 PCR \u524d\u548c PCR \u540e\u64cd\u4f5c\u5206\u5f00\u3002\u5982\u679c\u4f7f\u7528 OT-2 \u6a21\u5757\u8bbe\u7f6e PCR \u540e\u53cd\u5e94\uff08\u4f8b\u5982\u6d4b\u5e8f\uff09\uff0c\u5219\u4e0d\u5e94\u4f7f\u7528\u5b83\u4ece\u6837\u672c\u4e2d\u63d0\u53d6\u6838\u9178\uff0c\u9664\u975e\u5f7b\u5e95\u51c0\u5316\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u7814\u7a76\u4f18\u52bf\u548c\u5c40\u9650\u6027<\/h3>\n\n\n\n<p>\u8fd9\u9879\u7814\u7a76\u7684\u4e3b\u8981\u4f18\u52bf\u5728\u4e8e\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u90fd\u662f\u5728\u4e34\u5e8a\u5fae\u751f\u7269\u5b9e\u9a8c\u5ba4\u4e2d\u7528\u771f\u5b9e\u6837\u672c\u8fdb\u884c\u6d4b\u8bd5\u7684\u3002\u65b9\u6cd5\u4e4b\u95f4\u7684\u6bd4\u8f83\u5e76\u4e0d\u7cfb\u7edf\uff0c\u8fd9\u662f\u4e00\u4e2a\u91cd\u8981\u7684\u9650\u5236\u3002\u4e8b\u5b9e\u4e0a\uff0c\u8bbe\u8ba1\u7684\u76ee\u7684\u662f\u4f18\u5316\u6bcf\u4e2a\u7cfb\u7edf\u7684\u7ed3\u679c\uff0c\u56e0\u6b64\u8f93\u5165\u662f\u4e0d\u540c\u7684\u3002\u7cfb\u7edf\u5730\u63a2\u7d22\u8f93\u5165\u6837\u672c\u91cf\u4ee5\u53ca\u5176\u4ed6\u8bd5\u5242\u91cf\u5c06\u662f\u6709\u76ca\u548c\u6709\u76ca\u7684\uff0c\u4f46\u8fd9\u8d85\u51fa\u4e86\u8fd9\u9879\u7814\u7a76\u7684\u76ee\u7684\uff0c\u5373\u6bd4\u8f83\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\u73af\u5883\u4e2d\u7cfb\u7edf\u4e0e\u771f\u5b9e\u6837\u672c\u7684\u6027\u80fd\u3002<\/p>\n\n\n\n<h2 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