{"id":3515,"date":"2024-07-26T18:09:23","date_gmt":"2024-07-26T10:09:23","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3515"},"modified":"2024-09-02T18:22:22","modified_gmt":"2024-09-02T10:22:22","slug":"dierdaicexuyuanlitedian","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/dierdaicexuyuanlitedian\/","title":{"rendered":"\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u539f\u7406\u548c\u7279\u70b9"},"content":{"rendered":"\n
\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u4f5c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u7814\u7a76\u9886\u57df\u7684\u4e00\u573a\u9769\u547d\u6027\u98de\u8dc3\uff0c\u5176\u539f\u7406\u4e0e\u7279\u70b9\u6df1\u523b\u5730\u91cd\u5851\u4e86\u6211\u4eec\u5bf9\u9057\u4f20\u4fe1\u606f\u7684\u7406\u89e3\u4e0e\u5e94\u7528\u3002\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\uff08Next-generation sequencing, NGS\uff09\u7684\u539f\u7406\u548c\u7279\u70b9\u53ef\u4ee5\u8be6\u7ec6\u9610\u8ff0\u5982\u4e0b\uff1a<\/p>\n\n\n\n<\/figure>\n\n\n\n\u4e00\u3001\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u539f\u7406<\/p>\n\n\n\n\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u4e3b\u8981\u57fa\u4e8e\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\uff08Sequencing by Synthesis, SBS\uff09\u7684\u539f\u7406\u3002\u8fd9\u79cd\u6d4b\u5e8f\u65b9\u6cd5\u901a\u8fc7\u6355\u6349\u65b0\u6dfb\u52a0\u7684\u78b1\u57fa\u6240\u643a\u5e26\u7684\u7279\u6b8a\u6807\u8bb0\u6765\u786e\u5b9aDNA\u5e8f\u5217\u3002\u5177\u4f53\u6765\u8bf4\uff0c\u5728\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\uff0c\u4f1a\u5411DNA\u6a21\u677f\u94fe\u4e2d\u52a0\u5165\u7ecf\u8fc7\u7279\u6b8a\u6539\u9020\u7684DNA\u805a\u5408\u9176\u548c\u5e26\u6709\u53ef\u9006\u7ec8\u6b62\u8367\u5149\u6807\u8bb0\u7684dNTP\uff08\u8131\u6c27\u6838\u7cd6\u6838\u82f7\u4e09\u78f7\u9178\uff09\u3002\u8fd9\u4e9bdNTP\u7684\u6bcf\u4e2a\u78b1\u57fa\u90fd\u8fde\u63a5\u6709\u4e00\u4e2a\u4e0d\u540c\u7684\u8367\u5149\u57fa\u56e2\uff0c\u5e76\u4e14\u5b83\u4eec\u76843’\u7f9f\u57fa\u88ab\u4fee\u9970\uff0c\u4f7f\u5f97\u6bcf\u6b21\u53ea\u80fd\u52a0\u5165\u4e00\u4e2a\u78b1\u57fa\u3002\u5f53DNA\u805a\u5408\u9176\u50ac\u5316\u52a0\u5165\u4e00\u4e2adNTP\u65f6\uff0c\u8367\u5149\u57fa\u56e2\u4f1a\u53d1\u51fa\u7279\u5b9a\u989c\u8272\u7684\u5149\uff0c\u4ece\u800c\u88ab\u68c0\u6d4b\u5668\u6355\u83b7\u5e76\u8bb0\u5f55\u3002\u968f\u540e\uff0c\u901a\u8fc7\u5316\u5b66\u65b9\u6cd5\u53bb\u9664\u8367\u5149\u57fa\u56e2\u548c\u963b\u6b62\u94fe\u5ef6\u4f38\u7684\u57fa\u56e2\uff0c\u6062\u590d3’\u7f9f\u57fa\u7684\u6d3b\u6027\uff0c\u8fdb\u884c\u4e0b\u4e00\u8f6e\u6d4b\u5e8f\u53cd\u5e94\u3002<\/p>\n\n\n\n\u4e8c\u3001\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u7279\u70b9<\/p>\n\n\n\n1\u3001\u9ad8\u901a\u91cf\uff1aNGS\u80fd\u591f\u540c\u65f6\u5904\u7406\u6570\u767e\u4e07\u5230\u6570\u5341\u4ebf\u4e2aDNA\u7247\u6bb5\uff0c\u5927\u5927\u63d0\u9ad8\u4e86\u6d4b\u5e8f\u7684\u901a\u91cf\u548c\u6548\u7387\u3002\u8fd9\u4f7f\u5f97\u5728\u77ed\u65f6\u95f4\u5185\u5bf9\u5927\u91cf\u6837\u672c\u8fdb\u884c\u6d4b\u5e8f\u6210\u4e3a\u53ef\u80fd\uff0c\u4ece\u800c\u52a0\u901f\u4e86\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\u7b49\u9886\u57df\u7684\u7814\u7a76\u8fdb\u7a0b\u3002<\/p>\n\n\n\n2\u3001\u9ad8\u7075\u654f\u5ea6\uff1aNGS\u6280\u672f\u80fd\u591f\u68c0\u6d4b\u5230\u6781\u4f4e\u6d53\u5ea6\u7684DNA\u6216RNA\u5206\u5b50\uff0c\u751a\u81f3\u80fd\u591f\u533a\u5206\u5355\u5206\u5b50\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u8fd9\u4f7f\u5f97\u5b83\u5728\u9057\u4f20\u75be\u75c5\u8bca\u65ad\u3001\u80bf\u7624\u65e9\u671f\u68c0\u6d4b\u7b49\u9886\u57df\u5177\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\u524d\u666f\u3002<\/p>\n\n\n\n3\u3001\u8bfb\u957f\u77ed\uff1a\u867d\u7136NGS\u7684\u901a\u91cf\u5f88\u9ad8\uff0c\u4f46\u5176\u5355\u6b21\u6d4b\u5e8f\u7684\u8bfb\u957f\u76f8\u5bf9\u8f83\u77ed\uff08\u4e00\u822c\u4e3a\u51e0\u5341\u5230\u51e0\u767e\u4e2a\u78b1\u57fa\u5bf9\uff09\u3002\u8fd9\u610f\u5473\u7740\u5728\u6d4b\u5e8f\u5b8c\u6574\u57fa\u56e0\u7ec4\u6216\u8f6c\u5f55\u7ec4\u65f6\uff0c\u9700\u8981\u5c06DNA\u6216RNA\u7247\u6bb5\u6253\u65ad\u6210\u8f83\u5c0f\u7684\u7247\u6bb5\u8fdb\u884c\u6d4b\u5e8f\uff0c\u5e76\u5728\u6d4b\u5e8f\u5b8c\u6210\u540e\u8fdb\u884c\u62fc\u63a5\u3002\u56e0\u6b64\uff0cNGS\u6570\u636e\u7684\u51c6\u786e\u6027\u548c\u5b8c\u6574\u6027\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u53d6\u51b3\u4e8e\u62fc\u63a5\u7b97\u6cd5\u548c\u53c2\u6570\u7684\u8bbe\u7f6e\u3002<\/p>\n\n\n\n4\u3001\u6210\u672c\u4f4e\uff1a\u76f8\u8f83\u4e8e\u4f20\u7edf\u7684\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\uff08\u5982Sanger\u6d4b\u5e8f\uff09\uff0cNGS\u5927\u5927\u964d\u4f4e\u4e86\u6d4b\u5e8f\u6210\u672c\u3002\u8fd9\u4f7f\u5f97\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3001\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u7b49\u7814\u7a76\u53d8\u5f97\u66f4\u52a0\u7ecf\u6d4e\u53ef\u884c\u3002<\/p>\n\n\n\n5\u3001\u591a\u6837\u5316\u5e73\u53f0\uff1a\u76ee\u524d\u5e02\u573a\u4e0a\u5b58\u5728\u591a\u79cd\u57fa\u4e8e\u4e0d\u540c\u539f\u7406\u7684NGS\u5e73\u53f0\uff0c\u5982Illumina\u7684HiSeq\u548cMiSeq\u7cfb\u5217\u3001Thermo Fisher Scientific\u7684Ion Torrent\u7cfb\u5217\u4ee5\u53caOxford Nanopore Technologies\u7684MinION\u7b49\u3002\u8fd9\u4e9b\u5e73\u53f0\u5404\u6709\u4f18\u7f3a\u70b9\uff0c\u9002\u7528\u4e8e\u4e0d\u540c\u7684\u7814\u7a76\u9700\u6c42\u548c\u5e94\u7528\u573a\u666f\u3002<\/p>\n\n\n\n\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u57fa\u4e8e\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\u7684\u5de7\u5999\u539f\u7406\uff0c\u4e0d\u4ec5\u7b80\u5316\u4e86\u6d4b\u5e8f\u6d41\u7a0b\uff0c\u66f4\u6781\u5927\u5730\u63d0\u5347\u4e86\u6d4b\u5e8f\u7684\u901a\u91cf\u4e0e\u6548\u7387\uff0c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\u7b49\u9886\u57df\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u524d\u6240\u672a\u6709\u7684\u6570\u636e\u652f\u6301\u3002<\/p>\n\n\n\n\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684\u7279\u70b9\uff0c\u5219\u8fdb\u4e00\u6b65\u5de9\u56fa\u4e86\u5176\u5728\u751f\u547d\u79d1\u5b66\u9886\u57df\u7684\u6838\u5fc3\u5730\u4f4d\u3002\u9ad8\u901a\u91cf\u3001\u9ad8\u7075\u654f\u5ea6\u3001\u6210\u672c\u6548\u76ca\u6bd4\u7684\u4e0d\u65ad\u4f18\u5316\uff0c\u4ee5\u53ca\u591a\u6837\u5316\u5e73\u53f0\u7684\u4e0d\u65ad\u6d8c\u73b0\uff0c\u5171\u540c\u6784\u5efa\u4e86\u4e00\u4e2a\u5f3a\u5927\u4e14\u7075\u6d3b\u7684\u6d4b\u5e8f\u751f\u6001\u7cfb\u7edf\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u4f5c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u7814\u7a76\u9886\u57df\u7684\u4e00\u573a\u9769\u547d\u6027\u98de\u8dc3\uff0c\u5176\u539f\u7406\u4e0e\u7279\u70b9\u6df1\u523b\u5730\u91cd\u5851\u4e86\u6211\u4eec\u5bf9\u9057\u4f20\u4fe1\u606f\u7684\u7406\u89e3\u4e0e\u5e94\u7528\u3002\u7b2c\u4e8c […]<\/p>\n","protected":false},"author":5,"featured_media":3517,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3515","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"\n\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u539f\u7406\u548c\u7279\u70b9 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n\n\n\n\n\n\n\n\n\n\n\n\t\n\t\n\t\n\n\n\n\t\n\t\n\t\n
\u4e00\u3001\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u539f\u7406<\/p>\n\n\n\n
\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u4e3b\u8981\u57fa\u4e8e\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\uff08Sequencing by Synthesis, SBS\uff09\u7684\u539f\u7406\u3002\u8fd9\u79cd\u6d4b\u5e8f\u65b9\u6cd5\u901a\u8fc7\u6355\u6349\u65b0\u6dfb\u52a0\u7684\u78b1\u57fa\u6240\u643a\u5e26\u7684\u7279\u6b8a\u6807\u8bb0\u6765\u786e\u5b9aDNA\u5e8f\u5217\u3002\u5177\u4f53\u6765\u8bf4\uff0c\u5728\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\uff0c\u4f1a\u5411DNA\u6a21\u677f\u94fe\u4e2d\u52a0\u5165\u7ecf\u8fc7\u7279\u6b8a\u6539\u9020\u7684DNA\u805a\u5408\u9176\u548c\u5e26\u6709\u53ef\u9006\u7ec8\u6b62\u8367\u5149\u6807\u8bb0\u7684dNTP\uff08\u8131\u6c27\u6838\u7cd6\u6838\u82f7\u4e09\u78f7\u9178\uff09\u3002\u8fd9\u4e9bdNTP\u7684\u6bcf\u4e2a\u78b1\u57fa\u90fd\u8fde\u63a5\u6709\u4e00\u4e2a\u4e0d\u540c\u7684\u8367\u5149\u57fa\u56e2\uff0c\u5e76\u4e14\u5b83\u4eec\u76843’\u7f9f\u57fa\u88ab\u4fee\u9970\uff0c\u4f7f\u5f97\u6bcf\u6b21\u53ea\u80fd\u52a0\u5165\u4e00\u4e2a\u78b1\u57fa\u3002\u5f53DNA\u805a\u5408\u9176\u50ac\u5316\u52a0\u5165\u4e00\u4e2adNTP\u65f6\uff0c\u8367\u5149\u57fa\u56e2\u4f1a\u53d1\u51fa\u7279\u5b9a\u989c\u8272\u7684\u5149\uff0c\u4ece\u800c\u88ab\u68c0\u6d4b\u5668\u6355\u83b7\u5e76\u8bb0\u5f55\u3002\u968f\u540e\uff0c\u901a\u8fc7\u5316\u5b66\u65b9\u6cd5\u53bb\u9664\u8367\u5149\u57fa\u56e2\u548c\u963b\u6b62\u94fe\u5ef6\u4f38\u7684\u57fa\u56e2\uff0c\u6062\u590d3’\u7f9f\u57fa\u7684\u6d3b\u6027\uff0c\u8fdb\u884c\u4e0b\u4e00\u8f6e\u6d4b\u5e8f\u53cd\u5e94\u3002<\/p>\n\n\n\n
\u4e8c\u3001\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u7279\u70b9<\/p>\n\n\n\n
1\u3001\u9ad8\u901a\u91cf\uff1aNGS\u80fd\u591f\u540c\u65f6\u5904\u7406\u6570\u767e\u4e07\u5230\u6570\u5341\u4ebf\u4e2aDNA\u7247\u6bb5\uff0c\u5927\u5927\u63d0\u9ad8\u4e86\u6d4b\u5e8f\u7684\u901a\u91cf\u548c\u6548\u7387\u3002\u8fd9\u4f7f\u5f97\u5728\u77ed\u65f6\u95f4\u5185\u5bf9\u5927\u91cf\u6837\u672c\u8fdb\u884c\u6d4b\u5e8f\u6210\u4e3a\u53ef\u80fd\uff0c\u4ece\u800c\u52a0\u901f\u4e86\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\u7b49\u9886\u57df\u7684\u7814\u7a76\u8fdb\u7a0b\u3002<\/p>\n\n\n\n
2\u3001\u9ad8\u7075\u654f\u5ea6\uff1aNGS\u6280\u672f\u80fd\u591f\u68c0\u6d4b\u5230\u6781\u4f4e\u6d53\u5ea6\u7684DNA\u6216RNA\u5206\u5b50\uff0c\u751a\u81f3\u80fd\u591f\u533a\u5206\u5355\u5206\u5b50\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u8fd9\u4f7f\u5f97\u5b83\u5728\u9057\u4f20\u75be\u75c5\u8bca\u65ad\u3001\u80bf\u7624\u65e9\u671f\u68c0\u6d4b\u7b49\u9886\u57df\u5177\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\u524d\u666f\u3002<\/p>\n\n\n\n
3\u3001\u8bfb\u957f\u77ed\uff1a\u867d\u7136NGS\u7684\u901a\u91cf\u5f88\u9ad8\uff0c\u4f46\u5176\u5355\u6b21\u6d4b\u5e8f\u7684\u8bfb\u957f\u76f8\u5bf9\u8f83\u77ed\uff08\u4e00\u822c\u4e3a\u51e0\u5341\u5230\u51e0\u767e\u4e2a\u78b1\u57fa\u5bf9\uff09\u3002\u8fd9\u610f\u5473\u7740\u5728\u6d4b\u5e8f\u5b8c\u6574\u57fa\u56e0\u7ec4\u6216\u8f6c\u5f55\u7ec4\u65f6\uff0c\u9700\u8981\u5c06DNA\u6216RNA\u7247\u6bb5\u6253\u65ad\u6210\u8f83\u5c0f\u7684\u7247\u6bb5\u8fdb\u884c\u6d4b\u5e8f\uff0c\u5e76\u5728\u6d4b\u5e8f\u5b8c\u6210\u540e\u8fdb\u884c\u62fc\u63a5\u3002\u56e0\u6b64\uff0cNGS\u6570\u636e\u7684\u51c6\u786e\u6027\u548c\u5b8c\u6574\u6027\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u53d6\u51b3\u4e8e\u62fc\u63a5\u7b97\u6cd5\u548c\u53c2\u6570\u7684\u8bbe\u7f6e\u3002<\/p>\n\n\n\n
4\u3001\u6210\u672c\u4f4e\uff1a\u76f8\u8f83\u4e8e\u4f20\u7edf\u7684\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\uff08\u5982Sanger\u6d4b\u5e8f\uff09\uff0cNGS\u5927\u5927\u964d\u4f4e\u4e86\u6d4b\u5e8f\u6210\u672c\u3002\u8fd9\u4f7f\u5f97\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3001\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u7b49\u7814\u7a76\u53d8\u5f97\u66f4\u52a0\u7ecf\u6d4e\u53ef\u884c\u3002<\/p>\n\n\n\n
5\u3001\u591a\u6837\u5316\u5e73\u53f0\uff1a\u76ee\u524d\u5e02\u573a\u4e0a\u5b58\u5728\u591a\u79cd\u57fa\u4e8e\u4e0d\u540c\u539f\u7406\u7684NGS\u5e73\u53f0\uff0c\u5982Illumina\u7684HiSeq\u548cMiSeq\u7cfb\u5217\u3001Thermo Fisher Scientific\u7684Ion Torrent\u7cfb\u5217\u4ee5\u53caOxford Nanopore Technologies\u7684MinION\u7b49\u3002\u8fd9\u4e9b\u5e73\u53f0\u5404\u6709\u4f18\u7f3a\u70b9\uff0c\u9002\u7528\u4e8e\u4e0d\u540c\u7684\u7814\u7a76\u9700\u6c42\u548c\u5e94\u7528\u573a\u666f\u3002<\/p>\n\n\n\n
\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u57fa\u4e8e\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\u7684\u5de7\u5999\u539f\u7406\uff0c\u4e0d\u4ec5\u7b80\u5316\u4e86\u6d4b\u5e8f\u6d41\u7a0b\uff0c\u66f4\u6781\u5927\u5730\u63d0\u5347\u4e86\u6d4b\u5e8f\u7684\u901a\u91cf\u4e0e\u6548\u7387\uff0c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\u7b49\u9886\u57df\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u524d\u6240\u672a\u6709\u7684\u6570\u636e\u652f\u6301\u3002<\/p>\n\n\n\n
\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684\u7279\u70b9\uff0c\u5219\u8fdb\u4e00\u6b65\u5de9\u56fa\u4e86\u5176\u5728\u751f\u547d\u79d1\u5b66\u9886\u57df\u7684\u6838\u5fc3\u5730\u4f4d\u3002\u9ad8\u901a\u91cf\u3001\u9ad8\u7075\u654f\u5ea6\u3001\u6210\u672c\u6548\u76ca\u6bd4\u7684\u4e0d\u65ad\u4f18\u5316\uff0c\u4ee5\u53ca\u591a\u6837\u5316\u5e73\u53f0\u7684\u4e0d\u65ad\u6d8c\u73b0\uff0c\u5171\u540c\u6784\u5efa\u4e86\u4e00\u4e2a\u5f3a\u5927\u4e14\u7075\u6d3b\u7684\u6d4b\u5e8f\u751f\u6001\u7cfb\u7edf\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"
\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u4f5c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u7814\u7a76\u9886\u57df\u7684\u4e00\u573a\u9769\u547d\u6027\u98de\u8dc3\uff0c\u5176\u539f\u7406\u4e0e\u7279\u70b9\u6df1\u523b\u5730\u91cd\u5851\u4e86\u6211\u4eec\u5bf9\u9057\u4f20\u4fe1\u606f\u7684\u7406\u89e3\u4e0e\u5e94\u7528\u3002\u7b2c\u4e8c […]<\/p>\n","protected":false},"author":5,"featured_media":3517,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3515","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"\n