{"id":3558,"date":"2024-07-27T18:07:01","date_gmt":"2024-07-27T10:07:01","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3558"},"modified":"2024-09-02T18:33:42","modified_gmt":"2024-09-02T10:33:42","slug":"ngs-wenkugoujianzidonghua","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/ngs-wenkugoujianzidonghua\/","title":{"rendered":"NGS\u6587\u5e93\u6784\u5efa\u5728NGS\u81ea\u52a8\u5316\u5efa\u5e93\u4e2d\u7684\u5730\u4f4d"},"content":{"rendered":"\n

NGS\uff08Next Generation Sequencing\uff0c\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff09\u6587\u5e93\u6784\u5efa\u5728NGS\u81ea\u52a8\u5316\u5efa\u5e93\u4e2d\u7684\u5730\u4f4d\u662f\u81f3\u5173\u91cd\u8981\u7684\uff0c\u5b83\u662f\u6574\u4e2a\u6d4b\u5e8f\u6d41\u7a0b\u4e2d\u7684\u6838\u5fc3\u73af\u8282\uff0c\u76f4\u63a5\u51b3\u5b9a\u4e86\u540e\u7eed\u6d4b\u5e8f\u7684\u6210\u8d25\u548c\u6570\u636e\u7684\u8d28\u91cf\u3002<\/p>\n\n\n\n

\"NGS\u6587\u5e93\u6784\u5efa\"<\/figure>\n\n\n\n

\u4e00\u3001\u57fa\u7840\u4e0e\u524d\u63d0<\/p>\n\n\n\n

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1\u3001\u7247\u6bb5\u5316\u5904\u7406\uff1a\u5728\u6587\u5e93\u6784\u5efa\u4e2d\uff0cDNA\u6216RNA\u6837\u672c\u9700\u8981\u88ab\u5207\u5272\u6210\u9002\u5408\u6d4b\u5e8f\u7684\u7247\u6bb5\u5927\u5c0f\u3002\u8fd9\u4e00\u6b65\u9aa4\u662f\u540e\u7eed\u6d4b\u5e8f\u53cd\u5e94\u7684\u57fa\u7840\uff0c\u76f4\u63a5\u51b3\u5b9a\u4e86\u6d4b\u5e8f\u6570\u636e\u7684\u8986\u76d6\u5ea6\u548c\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n

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3\u3001\u6269\u589e\u4e0e\u8d28\u63a7\uff1a\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0c\u4e3a\u4e86\u83b7\u5f97\u8db3\u591f\u6570\u91cf\u7684DNA\u7247\u6bb5\u4ee5\u8fdb\u884c\u6d4b\u5e8f\uff0c\u53ef\u80fd\u9700\u8981\u8fdb\u884cPCR\u6269\u589e\u3002\u540c\u65f6\uff0c\u6587\u5e93\u8d28\u63a7\u4e5f\u662f\u786e\u4fdd\u6587\u5e93\u8d28\u91cf\u548c\u7eaf\u5ea6\u7684\u5173\u952e\u6b65\u9aa4\u3002\u8fd9\u4e9b\u64cd\u4f5c\u90fd\u5728\u6587\u5e93\u6784\u5efa\u8fc7\u7a0b\u4e2d\u5b8c\u6210\uff0c\u4e3a\u540e\u7eed\u6d4b\u5e8f\u63d0\u4f9b\u4e86\u9ad8\u8d28\u91cf\u7684\u6587\u5e93\u3002<\/p>\n\n\n\n

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2\u3001\u6d4b\u5e8f\u6548\u7387\uff1a\u9ad8\u8d28\u91cf\u7684\u6587\u5e93\u80fd\u591f\u63d0\u9ad8\u6d4b\u5e8f\u6548\u7387\uff0c\u51cf\u5c11\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u7684\u9519\u8bef\u548c\u6d6a\u8d39\u3002\u76f8\u53cd\uff0c\u8d28\u91cf\u4e0d\u4f73\u7684\u6587\u5e93\u53ef\u80fd\u5bfc\u81f4\u6d4b\u5e8f\u53cd\u5e94\u5931\u8d25\u6216\u6570\u636e\u8d28\u91cf\u4f4e\u4e0b\uff0c\u4ece\u800c\u589e\u52a0\u5b9e\u9a8c\u6210\u672c\u548c\u65f6\u95f4\u3002<\/p>\n\n\n\n

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1\u3001\u63d0\u9ad8\u6548\u7387\uff1aNGS\u81ea\u52a8\u5316\u5efa\u5e93\u7cfb\u7edf\u80fd\u591f\u6574\u5408\u591a\u4e2a\u6b65\u9aa4\uff0c\u51cf\u5c11\u4eba\u5de5\u5e72\u9884\u548c\u64cd\u4f5c\u65f6\u95f4\uff0c\u63d0\u9ad8\u5efa\u5e93\u6548\u7387\u3002<\/p>\n\n\n\n

2\u3001\u4fdd\u8bc1\u8d28\u91cf\uff1a\u81ea\u52a8\u5316\u5efa\u5e93\u7cfb\u7edf\u901a\u8fc7\u7cbe\u786e\u63a7\u5236\u53cd\u5e94\u6761\u4ef6\u548c\u51cf\u5c11\u4eba\u4e3a\u8bef\u5dee\uff0c\u80fd\u591f\u63d0\u9ad8\u6587\u5e93\u6784\u5efa\u7684\u8d28\u91cf\u548c\u53ef\u91cd\u590d\u6027\u3002<\/p>\n\n\n\n

3\u3001\u652f\u6301\u5927\u89c4\u6a21\u6d4b\u5e8f\uff1a\u81ea\u52a8\u5316\u5efa\u5e93\u7cfb\u7edf\u80fd\u591f\u5904\u7406\u5927\u91cf\u6837\u672c\uff0c\u6ee1\u8db3\u5927\u89c4\u6a21\u6d4b\u5e8f\u7684\u9700\u6c42\uff0c\u4e3a\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\u7b49\u9886\u57df\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u6709\u529b\u652f\u6301\u3002<\/p>\n\n\n\n

NGS\uff08\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff09\u6587\u5e93\u6784\u5efa\u5728NGS\u81ea\u52a8\u5316\u5efa\u5e93\u6d41\u7a0b\u4e2d\u5360\u636e\u7740\u4e3e\u8db3\u8f7b\u91cd\u7684\u5730\u4f4d\uff0c\u662f\u8fde\u63a5\u6837\u672c\u51c6\u5907\u4e0e\u9ad8\u901a\u91cf\u6d4b\u5e8f\u4e4b\u95f4\u7684\u5173\u952e\u6865\u6881\u3002\u5b83\u4e0d\u4ec5\u8981\u6c42\u9ad8\u8d28\u91cf\u7684DNA\u6216RNA\u6837\u672c\u4f5c\u4e3a\u8d77\u59cb\u6750\u6599\uff0c\u8fd8\u6d89\u53ca\u590d\u6742\u7684\u5206\u5b50\u751f\u7269\u5b66\u64cd\u4f5c\uff0c\u5982\u7247\u6bb5\u5316\u5904\u7406\u3001\u672b\u7aef\u4fee\u590d\u3001\u63a5\u5934\u8fde\u63a5\u4ee5\u53ca\u53ef\u80fd\u7684\u6269\u589e\u4e0e\u8d28\u63a7\u7b49\u6b65\u9aa4\u3002\u8fd9\u4e9b\u6b65\u9aa4\u5171\u540c\u786e\u4fdd\u4e86\u6587\u5e93\u80fd\u591f\u7b26\u5408\u6d4b\u5e8f\u5e73\u53f0\u7684\u8981\u6c42\uff0c\u4e3a\u540e\u7eed\u7684\u6d4b\u5e8f\u53cd\u5e94\u63d0\u4f9b\u4e86\u575a\u5b9e\u7684\u57fa\u7840\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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