{"id":4493,"date":"2024-09-28T16:26:07","date_gmt":"2024-09-28T08:26:07","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2024-09-28T17:44:30","modified_gmt":"2024-09-28T09:44:30","slug":"opentrons-flex-pcr-gongzuozhan","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/opentrons-flex-pcr-gongzuozhan\/","title":{"rendered":"Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684PCR\u53cd\u5e94"},"content":{"rendered":"\n<p>Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684\u5de5\u4f5c\u539f\u7406\u6df1\u523b\u6839\u690d\u4e8ePCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u8fd9\u4e00\u5206\u5b50\u751f\u7269\u5b66\u6280\u672f\u7684\u6838\u5fc3\u539f\u7406\u4e4b\u4e0a\uff0c\u540c\u65f6\u5de7\u5999\u5730\u878d\u5165\u4e86Opentrons\u5728\u81ea\u52a8\u5316\u5b9e\u9a8c\u5ba4\u8bbe\u5907\u7814\u53d1\u9886\u57df\u7684\u5c16\u7aef\u79d1\u6280\u4e0e\u521b\u65b0\u6210\u679c\u3002\u901a\u8fc7\u9ad8\u5ea6\u96c6\u6210\u7684\u81ea\u52a8\u5316\u7cfb\u7edf\uff0c\u8be5\u5de5\u4f5c\u7ad9\u4e0d\u4ec5\u7cbe\u51c6\u5730\u6a21\u62df\u4e86DNA\u5728\u81ea\u7136\u754c\u7684\u590d\u5236\u8fc7\u7a0b\uff0c\u8fd8\u6781\u5927\u5730\u63d0\u5347\u4e86\u5b9e\u9a8c\u7684\u7cbe\u786e\u5ea6\u3001\u53ef\u91cd\u590d\u6027\u548c\u6548\u7387\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"616\" height=\"481\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/09\/image-58.png\" alt=\"Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684PCR\u53cd\u5e94\" class=\"wp-image-4495\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/09\/image-58.png 616w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/09\/image-58-300x234.png 300w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/09\/image-58-576x450.png 576w\" sizes=\"(max-width: 616px) 100vw, 616px\" \/><\/figure>\n\n\n\n<p>\u4e00\u3001PCR\u53cd\u5e94\u6761\u4ef6<br>PCR\u53cd\u5e94\u6761\u4ef6\u5305\u62ec\u6e29\u5ea6\u3001\u65f6\u95f4\u548c\u5faa\u73af\u6b21\u6570\uff0c\u8fd9\u4e9b\u6761\u4ef6\u7684\u9009\u62e9\u548c\u4f18\u5316\u5bf9\u4e8ePCR\u53cd\u5e94\u7684\u6210\u529f\u81f3\u5173\u91cd\u8981\u3002<br>1\u3001\u6e29\u5ea6<br>\u53d8\u6027\u6e29\u5ea6\uff1a\u4e00\u822c\u4e3a90-95\u2103\uff0c\u7528\u4e8e\u4f7f\u6a21\u677fDNA\u5b8c\u5168\u53d8\u6027\u4e3a\u5355\u94fe\u3002<br>\u9000\u706b\u6e29\u5ea6\uff1a\u6839\u636e\u5f15\u7269\u7684Tm\u503c\u786e\u5b9a\uff0c\u4e00\u822c\u4e3a40-60\u2103\uff0c\u7528\u4e8e\u5f15\u7269\u4e0e\u6a21\u677fDNA\u7684\u7ed3\u5408\u3002<br>\u5ef6\u4f38\u6e29\u5ea6\uff1a\u4e00\u822c\u4e3a70-75\u2103\uff0c\u5728Taq DNA\u805a\u5408\u9176\u7684\u4f5c\u7528\u4e0b\u8fdb\u884cDNA\u94fe\u7684\u5ef6\u4f38\u3002<br>2\u3001\u65f6\u95f4<br>\u53d8\u6027\u65f6\u95f4\uff1a\u4e00\u822c\u8f83\u77ed\uff0c\u59821\u5206\u949f\uff0c\u8db3\u4ee5\u4f7f\u6a21\u677fDNA\u5b8c\u5168\u53d8\u6027\u3002<br>\u9000\u706b\u65f6\u95f4\uff1a\u4e00\u822c\u4e3a30-60\u79d2\uff0c\u8db3\u4ee5\u4f7f\u5f15\u7269\u4e0e\u6a21\u677fDNA\u5b8c\u5168\u7ed3\u5408\u3002<br>\u5ef6\u4f38\u65f6\u95f4\uff1a\u6839\u636e\u5f85\u6269\u589e\u7247\u6bb5\u7684\u957f\u5ea6\u800c\u5b9a\uff0c\u4e00\u822c\u4e3a1-15\u5206\u949f\u3002<br>3\u3001\u5faa\u73af\u6b21\u6570<br>\u4e00\u822c\u4e3a25-35\u6b21\uff0c\u5faa\u73af\u6b21\u6570\u8d8a\u591a\uff0c\u6269\u589e\u4ea7\u7269\u91cf\u8d8a\u5927\uff0c\u4f46\u4e5f\u53ef\u80fd\u589e\u52a0\u975e\u7279\u5f02\u6027\u4ea7\u7269\u7684\u4ea7\u751f\u3002<\/p>\n\n\n\n<p>\u4e8c\u3001\u51c6\u5907\u9636\u6bb5<br>1\u3001\u8bbe\u8ba1\u5f15\u7269\uff1a\u6839\u636e\u76ee\u6807DNA\u5e8f\u5217\u8bbe\u8ba1\u7279\u5f02\u6027\u5f15\u7269\uff0c\u786e\u4fdd\u5f15\u7269\u80fd\u591f\u9ad8\u6548\u5730\u4e0e\u6a21\u677fDNA\u7ed3\u5408\u5e76\u5f15\u5bfcDNA\u805a\u5408\u9176\u8fdb\u884c\u6269\u589e\u3002<br>2\u3001\u51c6\u5907\u6a21\u677fDNA\uff1a\u786e\u4fdd\u6a21\u677fDNA\u7684\u7eaf\u5ea6\u548c\u6d53\u5ea6\u8fbe\u5230PCR\u53cd\u5e94\u7684\u8981\u6c42\u3002\u6a21\u677fDNA\u53ef\u4ee5\u662f\u4ece\u751f\u7269\u6837\u672c\u4e2d\u63d0\u53d6\u7684\uff0c\u4e5f\u53ef\u4ee5\u662f\u5176\u4ed6\u6765\u6e90\u7684DNA\u7247\u6bb5\u3002<br>3\u3001\u914d\u5236\u53cd\u5e94\u4f53\u7cfb\uff1a\u6309\u7167PCR\u53cd\u5e94\u7684\u9700\u6c42\uff0c\u5c06\u6a21\u677fDNA\u3001\u5f15\u7269\u3001dNTPs\uff08\u8131\u6c27\u6838\u82f7\u4e09\u78f7\u9178\uff09\u3001\u7f13\u51b2\u6db2\u3001MgCl2\uff08\u5982\u679c\u9700\u8981\u7684\u8bdd\uff09\u3001DNA\u805a\u5408\u9176\u7b49\u6309\u4e00\u5b9a\u6bd4\u4f8b\u6df7\u5408\uff0c\u5f62\u6210PCR\u53cd\u5e94\u4f53\u7cfb\u3002\u8fd9\u4e00\u6b65\u9aa4\u9700\u8981\u5728\u65e0\u6838\u9178\u6c61\u67d3\u7684\u73af\u5883\u4e2d\u8fdb\u884c\uff0c\u4ee5\u9632\u6b62\u5916\u6765DNA\u7684\u5e72\u6270\u3002<br>4\u3001\u52a0\u8f7d\u6837\u672c\uff1a\u5c06\u914d\u5236\u597d\u7684PCR\u53cd\u5e94\u4f53\u7cfb\u52a0\u8f7d\u5230Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684\u6307\u5b9a\u4f4d\u7f6e\uff0c\u901a\u5e38\u662fPCR\u677f\u6216PCR\u7ba1\u4e2d\u3002\u786e\u4fdd\u6bcf\u4e2a\u5b54\u6216\u7ba1\u4e2d\u7684\u53cd\u5e94\u4f53\u7cfb\u4f53\u79ef\u548c\u6210\u5206\u4e00\u81f4\u3002<\/p>\n\n\n\n<p>\u4e09\u3001\u7a0b\u5e8f\u8bbe\u7f6e<br>1\u3001\u8fde\u63a5\u8bbe\u5907\uff1a\u5c06Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u4e0e\u8ba1\u7b97\u673a\u6216\u5176\u4ed6\u63a7\u5236\u8bbe\u5907\u8fde\u63a5\uff0c\u786e\u4fdd\u8bbe\u5907\u80fd\u591f\u6b63\u5e38\u901a\u4fe1\u3002<br>2\u3001\u542f\u52a8\u8f6f\u4ef6\uff1a\u6253\u5f00Opentrons\u63d0\u4f9b\u7684\u63a7\u5236\u8f6f\u4ef6\u6216\u754c\u9762\uff0c\u51c6\u5907\u8bbe\u7f6ePCR\u53cd\u5e94\u7a0b\u5e8f\u3002<br>3\u3001\u65b0\u5efaPCR\u7a0b\u5e8f\uff1a\u5728\u8f6f\u4ef6\u4e2d\u65b0\u5efa\u4e00\u4e2aPCR\u7a0b\u5e8f\uff0c\u8bbe\u7f6e\u5305\u62ec\u53d8\u6027\u3001\u9000\u706b\u3001\u5ef6\u4f38\u4e09\u4e2a\u6b65\u9aa4\u7684\u6e29\u5ea6\u3001\u65f6\u95f4\u548c\u5faa\u73af\u6b21\u6570\u7b49\u53c2\u6570\u3002\u8fd9\u4e9b\u53c2\u6570\u7684\u8bbe\u7f6e\u5e94\u57fa\u4e8e\u76ee\u6807DNA\u5e8f\u5217\u7684\u7279\u6027\u548c\u6240\u4f7f\u7528\u7684\u5f15\u7269\u3002Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u901a\u5e38\u652f\u6301\u7075\u6d3b\u7684\u7f16\u7a0b\u529f\u80fd\uff0c\u53ef\u4ee5\u6839\u636e\u5b9e\u9a8c\u9700\u6c42\u8fdb\u884c\u81ea\u5b9a\u4e49\u8bbe\u7f6e\u3002<br>4\u3001\u4fdd\u5b58\u5e76\u4e0a\u4f20\u7a0b\u5e8f\uff1a\u5c06\u8bbe\u7f6e\u597d\u7684PCR\u7a0b\u5e8f\u4fdd\u5b58\u5e76\u4e0a\u4f20\u5230Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u4e2d\u3002\u786e\u4fdd\u7a0b\u5e8f\u65e0\u8bef\u540e\uff0c\u51c6\u5907\u6267\u884cPCR\u53cd\u5e94\u3002<\/p>\n\n\n\n<p>\u56db\u3001\u6267\u884cPCR\u53cd\u5e94<br>1\u3001\u542f\u52a8\u53cd\u5e94\uff1a\u5728\u8f6f\u4ef6\u754c\u9762\u4e2d\u70b9\u51fb\u201c\u5f00\u59cb\u201d\u6216\u7c7b\u4f3c\u6309\u94ae\uff0c\u542f\u52a8PCR\u53cd\u5e94\u3002Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u5c06\u81ea\u52a8\u6309\u7167\u9884\u8bbe\u7684\u7a0b\u5e8f\u8fdb\u884c\u6e29\u5ea6\u63a7\u5236\u548c\u5faa\u73af\u64cd\u4f5c\u3002<br>2\u3001\u76d1\u63a7\u53cd\u5e94\uff1a\u5728\u53cd\u5e94\u8fc7\u7a0b\u4e2d\uff0c\u53ef\u4ee5\u901a\u8fc7\u8f6f\u4ef6\u754c\u9762\u5b9e\u65f6\u76d1\u63a7\u53cd\u5e94\u72b6\u6001\u548c\u8fdb\u5ea6\u3002Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u901a\u5e38\u5177\u6709\u5f3a\u5927\u7684\u76d1\u63a7\u529f\u80fd\uff0c\u80fd\u591f\u5b9e\u65f6\u663e\u793a\u6e29\u5ea6\u66f2\u7ebf\u3001\u5faa\u73af\u6b21\u6570\u7b49\u5173\u952e\u4fe1\u606f\u3002<br>3\u3001\u7ed3\u675f\u53cd\u5e94\uff1a\u5f53PCR\u53cd\u5e94\u8fbe\u5230\u9884\u8bbe\u7684\u5faa\u73af\u6b21\u6570\u540e\uff0cOpentrons Flex PCR \u5de5\u4f5c\u7ad9\u5c06\u81ea\u52a8\u505c\u6b62\u53cd\u5e94\u3002\u6b64\u65f6\u53ef\u4ee5\u53d6\u51faPCR\u677f\u6216PCR\u7ba1\u8fdb\u884c\u540e\u7eed\u5206\u6790\u3002<\/p>\n\n\n\n<p>\u4e94\u3001\u540e\u7eed\u5904\u7406<br>1\u3001\u5206\u6790PCR\u4ea7\u7269\uff1a\u901a\u8fc7\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u6216\u5176\u4ed6\u9002\u5f53\u7684\u65b9\u6cd5\u5206\u6790PCR\u4ea7\u7269\u7684\u7279\u5f02\u6027\u548c\u4ea7\u91cf\u3002\u786e\u4fdd\u6269\u589e\u4ea7\u7269\u7b26\u5408\u9884\u671f\u7684\u5927\u5c0f\u548c\u6570\u91cf\u3002<br>2\u3001\u6570\u636e\u8bb0\u5f55\u4e0e\u6574\u7406\uff1a\u8bb0\u5f55PCR\u53cd\u5e94\u7684\u7ed3\u679c\u548c\u6570\u636e\uff0c\u5e76\u8fdb\u884c\u5fc5\u8981\u7684\u6574\u7406\u548c\u5206\u6790\u3002\u8fd9\u4e9b\u6570\u636e\u53ef\u4ee5\u7528\u4e8e\u540e\u7eed\u7684\u5b9e\u9a8c\u9a8c\u8bc1\u6216\u79d1\u5b66\u7814\u7a76\u3002<br>3\u3001\u6e05\u6d01\u4e0e\u7ef4\u62a4\uff1a\u5728\u5b8c\u6210PCR\u53cd\u5e94\u540e\uff0c\u53ca\u65f6\u6e05\u6d01Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u548c\u76f8\u5173\u8bbe\u5907\uff0c\u4ee5\u9632\u6b62\u6c61\u67d3\u548c\u635f\u574f\u3002\u540c\u65f6\uff0c\u5b9a\u671f\u68c0\u67e5\u8bbe\u5907\u7684\u6027\u80fd\u548c\u72b6\u6001\uff0c\u786e\u4fdd\u8bbe\u5907\u80fd\u591f\u957f\u671f\u7a33\u5b9a\u8fd0\u884c\u3002<\/p>\n\n\n\n<p>\u8bf7\u6ce8\u610f\uff0c\u4ee5\u4e0a\u6b65\u9aa4\u662f\u57fa\u4e8e\u4e00\u822cPCR\u53cd\u5e94\u539f\u7406\u548cOpentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684\u529f\u80fd\u7279\u70b9\u8fdb\u884c\u7684\u6982\u62ec\u6027\u6307\u5bfc\u3002\u5177\u4f53\u64cd\u4f5c\u65f6\uff0c\u5e94\u53c2\u8003Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684\u7528\u6237\u624b\u518c\u6216\u8054\u7cfbOpentrons\u7684\u6280\u672f\u652f\u6301\u56e2\u961f\u4ee5\u83b7\u53d6\u51c6\u786e\u7684\u6307\u5bfc\u3002<\/p>\n\n\n\n<p>\u76f8\u5173\u9605\u8bfb<\/p>\n\n\n\n<p class=\"has-text-align-left\"><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/laboratory-automation\/\">\u6253\u9020\u5b9e\u9a8c\u5ba4\u81ea\u52a8\u5316\u201cPC\u201d<\/a><\/p>\n\n\n\n<p class=\"has-text-align-left\"><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/bpsopentronsgjdyyq\/\">\u767d\u76ae\u4e66\uff1aOpentrons\u9ad8\u7cbe\u5ea6\u79fb\u6db2\u5668<\/a><\/p>\n\n\n\n<p class=\"has-text-align-left\"><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/yyqbpswkmk\/\">\u79fb\u6db2\u767d\u76ae\u4e66\uff1a\u6e29\u63a7\u6a21\u5757<\/a><\/p>\n\n\n\n<p class=\"has-text-align-left\"><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/yyjqryqfwgs\/\">\u79fb\u6db2\u673a\u5668\u4eba\u4eea\u5668\u670d\u52a1\u6982\u8ff0<\/a><\/p>\n\n\n\n<p class=\"has-text-align-left\"><a href=\"https:\/\/opentrons.com.cn\/news\/opentrons-changyexian\/\">Opentrons\u6709\u54ea\u4e9b\u4ea7\u54c1\u7ebf<\/a><\/p>\n\n\n\n<p class=\"has-text-align-left\"><a href=\"https:\/\/opentrons.com.cn\/news\/yetigongzhuozhanyoudian\/\">\u6db2\u4f53\u5de5\u4f5c\u7ad9\u7684\u4f18\u70b9\u662f\u4ec0\u4e48<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Opentrons Flex PCR \u5de5\u4f5c\u7ad9\u7684\u5de5\u4f5c\u539f\u7406\u6df1\u523b\u6839\u690d\u4e8ePCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u8fd9\u4e00\u5206\u5b50\u751f\u7269\u5b66\u6280\u672f\u7684\u6838 [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":4495,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-4493","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles"],"yoast_head":"<!-- This site is optimized with the 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