{"id":8517,"date":"2025-01-19T18:18:54","date_gmt":"2025-01-19T10:18:54","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-01-19T18:19:26","modified_gmt":"2025-01-19T10:19:26","slug":"pcr-yinwushejiyuanli","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/","title":{"rendered":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406"},"content":{"rendered":"\n<p>\u5728\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\uff0cPCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u6280\u672f\u662f\u6700\u4e3a\u5e38\u7528\u4e14\u91cd\u8981\u7684\u5de5\u5177\u4e4b\u4e00\uff0c\u800cPCR\u5f15\u7269\u7684\u8bbe\u8ba1\u5219\u662f\u786e\u4fddPCR\u5b9e\u9a8c\u6210\u529f\u7684\u5173\u952e\u56e0\u7d20\u4e4b\u4e00\u3002PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406\u76f4\u63a5\u5173\u7cfb\u5230\u5b9e\u9a8c\u7684\u51c6\u786e\u6027\u3001\u7279\u5f02\u6027\u4ee5\u53ca\u6269\u589e\u6548\u7387\uff0c\u56e0\u6b64\uff0c\u7406\u89e3\u548c\u638c\u63e1\u5f15\u7269\u8bbe\u8ba1\u7684\u57fa\u672c\u539f\u5219\uff0c\u5bf9\u4e8e\u79d1\u5b66\u7814\u7a76\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" width=\"554\" height=\"368\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png\" alt=\"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406\" class=\"wp-image-8519\" style=\"width:641px;height:auto\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png 554w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13-300x199.png 300w\" sizes=\"(max-width: 554px) 100vw, 554px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"https:\/\/opentrons.com.cn\/\">PCR\u5f15\u7269<\/a><\/p>\n\n\n\n<p>\u4e00\u3001PCR\u5f15\u7269\u7684\u57fa\u672c\u6982\u5ff5<br>PCR\u5f15\u7269\u662f\u4e00\u6bb5\u77ed\u7684DNA\u6216RNA\u7247\u6bb5\uff0c\u901a\u5e38\u957f\u5ea6\u572818-30\u4e2a\u78b1\u57fa\u4e4b\u95f4\u3002\u5b83\u4eec\u5728PCR\u53cd\u5e94\u4e2d\u8d77\u5230\u975e\u5e38\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u80fd\u591f\u5728\u6a21\u677fDNA\u7684\u4e24\u7aef\u7279\u5f02\u6027\u5730\u7ed3\u5408\uff0c\u5e76\u4e3aDNA\u805a\u5408\u9176\u63d0\u4f9b\u5ef6\u4f38\u7684\u8d77\u59cb\u70b9\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u5f15\u7269\u5c31\u50cf\u662fPCR\u5b9e\u9a8c\u7684\u201c\u8d77\u70b9\u6807\u8bb0\u201d\uff0c\u5b83\u4eec\u786e\u4fdd\u805a\u5408\u9176\u5728\u6b63\u786e\u7684\u4f4d\u7f6e\u5f00\u59cb\u6269\u589e\u3002<\/p>\n\n\n\n<p>\u4e8c\u3001PCR\u5f15\u7269\u8bbe\u8ba1\u7684\u57fa\u672c\u539f\u5219<br>1\u3001\u5f15\u7269\u957f\u5ea6\u7684\u9009\u62e9<br>\u5f15\u7269\u7684\u957f\u5ea6\u662f\u5f71\u54cdPCR\u5b9e\u9a8c\u6210\u529f\u7684\u4e00\u4e2a\u5173\u952e\u56e0\u7d20\u3002\u4e00\u822c\u6765\u8bf4\uff0c\u5f15\u7269\u7684\u957f\u5ea6\u9700\u8981\u572818\u523030\u4e2a\u78b1\u57fa\u4e4b\u95f4\uff0c\u8fc7\u77ed\u7684\u5f15\u7269\u53ef\u80fd\u5bfc\u81f4\u6269\u589e\u7279\u5f02\u6027\u5dee\uff0c\u8fc7\u957f\u5219\u53ef\u80fd\u4f1a\u5f15\u53d1\u975e\u7279\u5f02\u6027\u6269\u589e\u6216\u589e\u52a0\u5f15\u7269\u7684\u81ea\u6211\u7ed3\u5408\u3002\u5f15\u7269\u7684\u957f\u5ea6\u901a\u5e38\u4f1a\u6839\u636e\u76ee\u6807\u7247\u6bb5\u7684\u957f\u5ea6\u548c\u5b9e\u9a8c\u9700\u6c42\u8fdb\u884c\u8c03\u6574\u3002<br>2\u3001\u5f15\u7269\u7684GC\u542b\u91cf<br>GC\u542b\u91cf\u662f\u6307\u5f15\u7269\u4e2dG\uff08\u9e1f\u560c\u5464\uff09\u548cC\uff08\u80de\u5627\u5576\uff09\u7684\u6bd4\u4f8b\u3002\u7406\u60f3\u7684PCR\u5f15\u7269GC\u542b\u91cf\u5e94\u8be5\u572840%\u523060%\u4e4b\u95f4\u3002\u9002\u5f53\u7684GC\u542b\u91cf\u6709\u52a9\u4e8e\u5f15\u7269\u4e0e\u76ee\u6807DNA\u7684\u7ed3\u5408\uff0c\u540c\u65f6\u80fd\u591f\u63d0\u4f9b\u8f83\u9ad8\u7684\u7a33\u5b9a\u6027\u3002\u5982\u679cGC\u542b\u91cf\u8fc7\u9ad8\u6216\u8fc7\u4f4e\uff0c\u53ef\u80fd\u5bfc\u81f4\u5f15\u7269\u4e0e\u76ee\u6807DNA\u7684\u7ed3\u5408\u4e0d\u7a33\u5b9a\uff0c\u8fdb\u800c\u5f71\u54cdPCR\u6269\u589e\u6548\u7387\u3002<br>3\u3001\u5f15\u7269\u7684\u9000\u706b\u6e29\u5ea6\uff08Tm\uff09<br>\u5f15\u7269\u7684\u9000\u706b\u6e29\u5ea6\uff08Tm\uff09\u662f\u6307\u5f15\u7269\u4e0e\u6a21\u677fDNA\u5728\u7279\u5b9a\u6e29\u5ea6\u4e0b\u7ed3\u5408\u7684\u6e29\u5ea6\u3002\u4e3a\u4e86\u786e\u4fddPCR\u53cd\u5e94\u7684\u6709\u6548\u6027\uff0c\u5f15\u7269\u7684Tm\u503c\u5e94\u8be5\u76f8\u4f3c\uff0c\u6700\u597d\u76f8\u5dee\u4e0d\u8d85\u8fc72\u2103\u3002\u7406\u60f3\u7684Tm\u503c\u901a\u5e38\u572850\u2103\u523065\u2103\u4e4b\u95f4\u3002\u82e5\u5f15\u7269Tm\u503c\u5dee\u8ddd\u8fc7\u5927\uff0c\u4f1a\u5bfc\u81f4\u6269\u589e\u6548\u7387\u4f4e\u4e0b\uff0c\u751a\u81f3\u5931\u8d25\u3002<br>4\u3001\u907f\u514d\u5f15\u7269\u4e8c\u805a\u4f53\u548c\u53d1\u5939\u7ed3\u6784<br>\u5f15\u7269\u8bbe\u8ba1\u65f6\u9700\u8981\u907f\u514d\u5f15\u7269\u4e4b\u95f4\u7684\u4e8c\u805a\u4f53\u5f62\u6210\u3002\u4e8c\u805a\u4f53\u662f\u6307\u4e24\u4e2a\u5f15\u7269\u4e92\u76f8\u7ed3\u5408\u5f62\u6210\u4e0d\u5e0c\u671b\u7684\u4e8c\u7ea7\u7ed3\u6784\uff0c\u8fd9\u4f1a\u5f71\u54cd\u6269\u589e\u7684\u7279\u5f02\u6027\u548c\u6548\u7387\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u8fd8\u5e94\u907f\u514d\u5f15\u7269\u81ea\u6211\u7ed3\u5408\u5f62\u6210\u53d1\u5939\u7ed3\u6784\uff0c\u8fd9\u79cd\u7ed3\u6784\u4f1a\u5bfc\u81f4\u5f15\u7269\u7684\u7ed3\u5408\u6548\u7387\u964d\u4f4e\u3002\u56e0\u6b64\uff0c\u5728\u8bbe\u8ba1\u5f15\u7269\u65f6\uff0c\u4f7f\u7528\u5408\u9002\u7684\u8ba1\u7b97\u5de5\u5177\u9884\u6d4b\u5e76\u907f\u514d\u8fd9\u4e9b\u4e0d\u826f\u7ed3\u6784\u7684\u5f62\u6210\u975e\u5e38\u91cd\u8981\u3002<br>5\u3001\u5f15\u7269\u7684\u7279\u5f02\u6027\u8bbe\u8ba1<br>\u5f15\u7269\u7684\u7279\u5f02\u6027\u5bf9\u4e8ePCR\u7684\u6210\u529f\u81f3\u5173\u91cd\u8981\u3002\u8bbe\u8ba1\u5f15\u7269\u65f6\uff0c\u5fc5\u987b\u786e\u4fdd\u5f15\u7269\u80fd\u591f\u51c6\u786e\u5730\u7ed3\u5408\u5230\u76ee\u6807DNA\u7684\u7279\u5b9a\u533a\u57df\u3002\u4e3a\u4e86\u907f\u514d\u975e\u7279\u5f02\u6027\u6269\u589e\uff0c\u8bbe\u8ba1\u65f6\u5e94\u68c0\u67e5\u5f15\u7269\u7684\u5e8f\u5217\u662f\u5426\u4f1a\u4e0e\u975e\u76ee\u6807\u5e8f\u5217\u53d1\u751f\u7ed3\u5408\u3002\u8fd9\u901a\u5e38\u9700\u8981\u501f\u52a9\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177\uff0c\u5982BLAST\u8fdb\u884c\u5e8f\u5217\u6bd4\u5bf9\u5206\u6790\uff0c\u4ee5\u786e\u4fdd\u5f15\u7269\u7684\u7279\u5f02\u6027\u3002<\/p>\n\n\n\n<p>\u4e8c\u3001PCR\u5f15\u7269\u8bbe\u8ba1\u7684\u5de5\u5177\u4e0e\u8f6f\u4ef6<br>\u968f\u7740\u751f\u7269\u4fe1\u606f\u5b66\u6280\u672f\u7684\u53d1\u5c55\uff0c\u8bb8\u591a\u4e13\u4e1a\u7684PCR\u5f15\u7269\u8bbe\u8ba1\u8f6f\u4ef6\u548c\u5728\u7ebf\u5de5\u5177\u5e94\u8fd0\u800c\u751f\uff0c\u8fd9\u4e9b\u5de5\u5177\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u4eba\u5458\u5feb\u901f\u3001\u51c6\u786e\u5730\u8bbe\u8ba1PCR\u5f15\u7269\u3002\u4f8b\u5982\uff0cPrimer3\u3001OligoCalc\u548cPrimer-BLAST\u7b49\u5de5\u5177\uff0c\u80fd\u591f\u6839\u636e\u76ee\u6807\u5e8f\u5217\u81ea\u52a8\u8ba1\u7b97\u51fa\u6700\u4f73\u7684\u5f15\u7269\u957f\u5ea6\u3001GC\u542b\u91cf\u548c\u9000\u706b\u6e29\u5ea6\u7b49\u53c2\u6570\uff0c\u5e76\u9884\u6d4b\u53ef\u80fd\u5b58\u5728\u7684\u4e8c\u805a\u4f53\u6216\u53d1\u5939\u7ed3\u6784\uff0c\u4ece\u800c\u5927\u5927\u63d0\u9ad8\u5f15\u7269\u8bbe\u8ba1\u7684\u6548\u7387\u548c\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n<p>\u4e09\u3001PCR\u5f15\u7269\u8bbe\u8ba1\u4e2d\u7684\u5e38\u89c1\u95ee\u9898\u53ca\u89e3\u51b3\u65b9\u6848<br>1\u3001\u975e\u7279\u5f02\u6027\u6269\u589e\uff1a\u5982\u679cPCR\u53cd\u5e94\u4e2d\u51fa\u73b0\u4e86\u975e\u7279\u5f02\u6027\u6269\u589e\uff0c\u53ef\u80fd\u662f\u5f15\u7269\u4e0e\u6a21\u677fDNA\u7684\u7ed3\u5408\u4e0d\u591f\u7279\u5f02\u3002\u6b64\u65f6\u53ef\u4ee5\u5c1d\u8bd5\u8c03\u6574\u5f15\u7269\u7684\u5e8f\u5217\u3001\u4f18\u5316PCR\u6761\u4ef6\uff0c\u6216\u8005\u4f7f\u7528\u66f4\u52a0\u7279\u5f02\u7684\u5f15\u7269\u3002<br>2\u3001\u5f15\u7269\u4e8c\u805a\u4f53\u6216\u53d1\u5939\u7ed3\u6784\uff1a\u8fd9\u79cd\u95ee\u9898\u901a\u5e38\u53ef\u4ee5\u901a\u8fc7\u8c03\u6574\u5f15\u7269\u7684\u5e8f\u5217\uff0c\u907f\u514d\u91cd\u590d\u7684G\u6216C\u533a\u57df\uff0c\u6216\u8005\u91c7\u7528\u5408\u9002\u7684\u5f15\u7269\u8bbe\u8ba1\u8f6f\u4ef6\u8fdb\u884c\u4f18\u5316\u6765\u89e3\u51b3\u3002<br>3\u3001\u6269\u589e\u6548\u7387\u4f4e\u4e0b\uff1a\u4f4e\u6548\u7684\u6269\u589e\u53ef\u80fd\u662f\u7531\u4e8e\u5f15\u7269\u8bbe\u8ba1\u4e0d\u5f53\uff0c\u6216PCR\u6761\u4ef6\u4e0d\u5408\u9002\u3002\u53ef\u4ee5\u901a\u8fc7\u91cd\u65b0\u8bbe\u8ba1\u5f15\u7269\uff0c\u6216\u4f18\u5316PCR\u53cd\u5e94\u4f53\u7cfb\uff08\u5982\u9176\u3001\u6a21\u677f\u6d53\u5ea6\uff09\u6765\u63d0\u9ad8\u6548\u7387\u3002<\/p>\n\n\n\n<p>PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u662fPCR\u5b9e\u9a8c\u6210\u529f\u7684\u5173\u952e\u56e0\u7d20\u4e4b\u4e00\u3002\u4e00\u4e2a\u8bbe\u8ba1\u5408\u7406\u7684\u5f15\u7269\u4e0d\u4ec5\u80fd\u63d0\u9ad8\u5b9e\u9a8c\u7684\u7279\u5f02\u6027\u548c\u6269\u589e\u6548\u7387\uff0c\u8fd8\u80fd\u51cf\u5c11\u5b9e\u9a8c\u4e2d\u5e38\u89c1\u7684\u9519\u8bef\u548c\u95ee\u9898\u3002\u638c\u63e1PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406\uff0c\u5e76\u4f7f\u7528\u5408\u9002\u7684\u5de5\u5177\u548c\u65b9\u6cd5\u8fdb\u884c\u4f18\u5316\uff0c\u662f\u6bcf\u4e00\u4e2a\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4eba\u5458\u5fc5\u5907\u7684\u6280\u80fd\u3002<\/p>\n\n\n\n<p>\u76f8\u5173\u9605\u8bfb\u63a8\u8350<\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/yyqbpsshylxgj\/\">\u79fb\u6db2\u5668\u767d\u76ae\u4e66\uff1a\u56db\u5408\u4e00\u79bb\u5fc3\u7ba1\u67b6<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/bpsopentronsgjdyyq\/\">\u767d\u76ae\u4e66\uff1aOpentrons\u9ad8\u7cbe\u5ea6\u79fb\u6db2\u5668<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/ot-2hbsthp-1xbgyhqspdzdcd\/\">OT-2\u6d77\u62a5\u4e0aTHP-1\u7ec6\u80de\u8fc7\u6c27\u5316\u6c22\u6c34\u5e73\u7684\u81ea\u52a8\u6d4b\u5b9a<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/danbaizhichunhuaxitongyuanli\/\">\u86cb\u767d\u8d28\u7eaf\u5316\u7cfb\u7edf\u7684\u539f\u7406\u548c\u5e94\u7528<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/jiyinerdaicexuyuanli\/\">\u57fa\u56e0\u4e8c\u4ee3\u6d4b\u5e8f\u539f\u7406\u662f\u4ec0\u4e48<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/flex-gongzuozhanrouxingcikuai\/\">Flex\u8d28\u7c92\u5236\u5907\u5de5\u4f5c\u7ad9\u7684\u67d4\u6027\u78c1\u5757<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5728\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\uff0cPCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u6280\u672f\u662f\u6700\u4e3a\u5e38\u7528\u4e14\u91cd\u8981\u7684\u5de5\u5177\u4e4b\u4e00\uff0c\u800cPCR\u5f15\u7269\u7684\u8bbe\u8ba1\u5219\u662f\u786e\u4fddPCR\u5b9e [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":8519,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-8517","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-view-all"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406 - Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"og:description\" content=\"\u5728\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\uff0cPCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u6280\u672f\u662f\u6700\u4e3a\u5e38\u7528\u4e14\u91cd\u8981\u7684\u5de5\u5177\u4e4b\u4e00\uff0c\u800cPCR\u5f15\u7269\u7684\u8bbe\u8ba1\u5219\u662f\u786e\u4fddPCR\u5b9e [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\" \/>\n<meta property=\"og:site_name\" content=\"Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"article:published_time\" content=\"2025-01-19T10:18:54+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-01-19T10:19:26+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png\" \/>\n\t<meta property=\"og:image:width\" content=\"554\" \/>\n\t<meta property=\"og:image:height\" content=\"368\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Huang, Chris\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"Huang, Chris\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 \u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\"},\"author\":{\"name\":\"Huang, Chris\",\"@id\":\"https:\/\/opentrons.com.cn\/#\/schema\/person\/e96255ecc348c5e4e89a0541e7144cae\"},\"headline\":\"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406\",\"datePublished\":\"2025-01-19T10:18:54+00:00\",\"dateModified\":\"2025-01-19T10:19:26+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\"},\"wordCount\":60,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/opentrons.com.cn\/#organization\"},\"image\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png\",\"articleSection\":[\"\u5168\u90e8\"],\"inLanguage\":\"zh-Hans\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\",\"url\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\",\"name\":\"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406 - Opentrons\u4e2d\u56fd\u5b98\u7f51\",\"isPartOf\":{\"@id\":\"https:\/\/opentrons.com.cn\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png\",\"datePublished\":\"2025-01-19T10:18:54+00:00\",\"dateModified\":\"2025-01-19T10:19:26+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#breadcrumb\"},\"inLanguage\":\"zh-Hans\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"zh-Hans\",\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage\",\"url\":\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png\",\"contentUrl\":\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png\",\"width\":554,\"height\":368,\"caption\":\"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"\u9996\u9875\",\"item\":\"https:\/\/opentrons.com.cn\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/opentrons.com.cn\/#website\",\"url\":\"https:\/\/opentrons.com.cn\/\",\"name\":\"\u79fb\u6db2\u5de5\u4f5c\u7ad9-\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9-Opentrons\u5408\u521b\u751f\u7269\u5de5\u7a0b\uff08\u6df1\u5733\uff09\u6709\u9650\u516c\u53f8\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/opentrons.com.cn\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/opentrons.com.cn\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"zh-Hans\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/opentrons.com.cn\/#organization\",\"name\":\"\u79fb\u6db2\u5de5\u4f5c\u7ad9-\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9-Opentrons\u5408\u521b\u751f\u7269\u5de5\u7a0b\uff08\u6df1\u5733\uff09\u6709\u9650\u516c\u53f8\",\"url\":\"https:\/\/opentrons.com.cn\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"zh-Hans\",\"@id\":\"https:\/\/opentrons.com.cn\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/04\/cropped-FAVICON-1.webp\",\"contentUrl\":\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/04\/cropped-FAVICON-1.webp\",\"width\":512,\"height\":512,\"caption\":\"\u79fb\u6db2\u5de5\u4f5c\u7ad9-\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9-Opentrons\u5408\u521b\u751f\u7269\u5de5\u7a0b\uff08\u6df1\u5733\uff09\u6709\u9650\u516c\u53f8\"},\"image\":{\"@id\":\"https:\/\/opentrons.com.cn\/#\/schema\/logo\/image\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\/\/opentrons.com.cn\/#\/schema\/person\/e96255ecc348c5e4e89a0541e7144cae\",\"name\":\"Huang, Chris\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"zh-Hans\",\"@id\":\"https:\/\/opentrons.com.cn\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/2343185d23bd87161be946a1b4cb3c7a?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/2343185d23bd87161be946a1b4cb3c7a?s=96&d=mm&r=g\",\"caption\":\"Huang, Chris\"},\"url\":\"https:\/\/opentrons.com.cn\/author\/chris-huang\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406 - Opentrons\u4e2d\u56fd\u5b98\u7f51","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/","og_locale":"zh_CN","og_type":"article","og_title":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406 - Opentrons\u4e2d\u56fd\u5b98\u7f51","og_description":"\u5728\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\uff0cPCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u6280\u672f\u662f\u6700\u4e3a\u5e38\u7528\u4e14\u91cd\u8981\u7684\u5de5\u5177\u4e4b\u4e00\uff0c\u800cPCR\u5f15\u7269\u7684\u8bbe\u8ba1\u5219\u662f\u786e\u4fddPCR\u5b9e [&hellip;]","og_url":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/","og_site_name":"Opentrons\u4e2d\u56fd\u5b98\u7f51","article_published_time":"2025-01-19T10:18:54+00:00","article_modified_time":"2025-01-19T10:19:26+00:00","og_image":[{"width":554,"height":368,"url":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png","type":"image\/png"}],"author":"Huang, Chris","twitter_card":"summary_large_image","twitter_misc":{"\u4f5c\u8005":"Huang, Chris","\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4":"1 \u5206"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#article","isPartOf":{"@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/"},"author":{"name":"Huang, Chris","@id":"https:\/\/opentrons.com.cn\/#\/schema\/person\/e96255ecc348c5e4e89a0541e7144cae"},"headline":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406","datePublished":"2025-01-19T10:18:54+00:00","dateModified":"2025-01-19T10:19:26+00:00","mainEntityOfPage":{"@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/"},"wordCount":60,"commentCount":0,"publisher":{"@id":"https:\/\/opentrons.com.cn\/#organization"},"image":{"@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage"},"thumbnailUrl":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png","articleSection":["\u5168\u90e8"],"inLanguage":"zh-Hans","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/","url":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/","name":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406 - Opentrons\u4e2d\u56fd\u5b98\u7f51","isPartOf":{"@id":"https:\/\/opentrons.com.cn\/#website"},"primaryImageOfPage":{"@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage"},"image":{"@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage"},"thumbnailUrl":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png","datePublished":"2025-01-19T10:18:54+00:00","dateModified":"2025-01-19T10:19:26+00:00","breadcrumb":{"@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#breadcrumb"},"inLanguage":"zh-Hans","potentialAction":[{"@type":"ReadAction","target":["https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/"]}]},{"@type":"ImageObject","inLanguage":"zh-Hans","@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#primaryimage","url":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png","contentUrl":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/01\/image-13.png","width":554,"height":368,"caption":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406"},{"@type":"BreadcrumbList","@id":"https:\/\/opentrons.com.cn\/news\/pcr-yinwushejiyuanli\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"\u9996\u9875","item":"https:\/\/opentrons.com.cn\/"},{"@type":"ListItem","position":2,"name":"PCR\u5f15\u7269\u7684\u8bbe\u8ba1\u539f\u7406"}]},{"@type":"WebSite","@id":"https:\/\/opentrons.com.cn\/#website","url":"https:\/\/opentrons.com.cn\/","name":"\u79fb\u6db2\u5de5\u4f5c\u7ad9-\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9-Opentrons\u5408\u521b\u751f\u7269\u5de5\u7a0b\uff08\u6df1\u5733\uff09\u6709\u9650\u516c\u53f8","description":"","publisher":{"@id":"https:\/\/opentrons.com.cn\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/opentrons.com.cn\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"zh-Hans"},{"@type":"Organization","@id":"https:\/\/opentrons.com.cn\/#organization","name":"\u79fb\u6db2\u5de5\u4f5c\u7ad9-\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9-Opentrons\u5408\u521b\u751f\u7269\u5de5\u7a0b\uff08\u6df1\u5733\uff09\u6709\u9650\u516c\u53f8","url":"https:\/\/opentrons.com.cn\/","logo":{"@type":"ImageObject","inLanguage":"zh-Hans","@id":"https:\/\/opentrons.com.cn\/#\/schema\/logo\/image\/","url":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/04\/cropped-FAVICON-1.webp","contentUrl":"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/04\/cropped-FAVICON-1.webp","width":512,"height":512,"caption":"\u79fb\u6db2\u5de5\u4f5c\u7ad9-\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9-Opentrons\u5408\u521b\u751f\u7269\u5de5\u7a0b\uff08\u6df1\u5733\uff09\u6709\u9650\u516c\u53f8"},"image":{"@id":"https:\/\/opentrons.com.cn\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/opentrons.com.cn\/#\/schema\/person\/e96255ecc348c5e4e89a0541e7144cae","name":"Huang, Chris","image":{"@type":"ImageObject","inLanguage":"zh-Hans","@id":"https:\/\/opentrons.com.cn\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/2343185d23bd87161be946a1b4cb3c7a?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/2343185d23bd87161be946a1b4cb3c7a?s=96&d=mm&r=g","caption":"Huang, Chris"},"url":"https:\/\/opentrons.com.cn\/author\/chris-huang\/"}]}},"_links":{"self":[{"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/posts\/8517","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/comments?post=8517"}],"version-history":[{"count":1,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/posts\/8517\/revisions"}],"predecessor-version":[{"id":8520,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/posts\/8517\/revisions\/8520"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/media\/8519"}],"wp:attachment":[{"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/media?parent=8517"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/categories?post=8517"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/opentrons.com.cn\/wp-json\/wp\/v2\/tags?post=8517"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}