{"id":8616,"date":"2025-02-19T15:38:57","date_gmt":"2025-02-19T07:38:57","guid":{"rendered":"https:\/\/opentrons.com.cn\/news\/\/"},"modified":"2025-02-19T15:39:31","modified_gmt":"2025-02-19T07:39:31","slug":"pcr-tixigoujian","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/news\/pcr-tixigoujian\/","title":{"rendered":"PCR\u4f53\u7cfb\u6784\u5efa\u7684\u56db\u4e2a\u6b65\u9aa4"},"content":{"rendered":"\n<p>PCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u4e00\u79cd\u5e7f\u6cdb\u5e94\u7528\u7684\u6280\u672f\uff0c\u7528\u4e8e\u6269\u589e\u7279\u5b9a\u7684DNA\u5e8f\u5217\u3002\u65e0\u8bba\u662f\u57fa\u56e0\u514b\u9686\u3001\u7a81\u53d8\u5206\u6790\uff0c\u8fd8\u662f\u57fa\u56e0\u8868\u8fbe\u68c0\u6d4b\uff0cPCR\u90fd\u53d1\u6325\u7740\u4e0d\u53ef\u66ff\u4ee3\u7684\u4f5c\u7528\u3002\u6784\u5efa\u4e00\u4e2a\u9ad8\u6548\u4e14\u53ef\u9760\u7684PCR\u4f53\u7cfb\u662f\u4fdd\u8bc1\u5b9e\u9a8c\u6210\u529f\u7684\u5173\u952e\uff0c\u800c\u8fd9\u4e00\u8fc7\u7a0b\u901a\u5e38\u5305\u62ec\u56db\u4e2a\u91cd\u8981\u6b65\u9aa4\u3002\u672c\u6587\u5c06\u8be6\u7ec6\u4ecb\u7ecdPCR\u4f53\u7cfb\u6784\u5efa\u7684\u56db\u4e2a\u6b65\u9aa4\uff0c\u5e2e\u52a9\u5927\u5bb6\u66f4\u597d\u5730\u7406\u89e3\u548c\u5e94\u7528\u8fd9\u4e00\u6280\u672f\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"553\" height=\"312\" src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/02\/image-23.png\" alt=\"PCR\u4f53\u7cfb\u6784\u5efa\u7684\u56db\u4e2a\u6b65\u9aa4\" class=\"wp-image-8618\" srcset=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/02\/image-23.png 553w, https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/02\/image-23-300x169.png 300w\" sizes=\"(max-width: 553px) 100vw, 553px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"https:\/\/opentrons.com.cn\/\">PCR\u4f53\u7cfb\u6784\u5efa<\/a><\/p>\n\n\n\n<p><strong>\u4e00\u3001\u6a21\u677fDNA\u7684\u5236\u5907<\/strong><br>1\u3001\u76ee\u7684\uff1a\u4ece\u6837\u54c1\u4e2d\u63d0\u53d6\u51faDNA\uff0c\u4f5c\u4e3aPCR\u6269\u589e\u7684\u6a21\u677f\u3002<br>2\u3001\u65b9\u6cd5\uff1a\u5e38\u89c1\u7684\u63d0\u53d6\u65b9\u6cd5\u5305\u62ecCTAB\u6cd5\u3001\u76d0\u6eb6\u6cd5\u4ee5\u53ca\u4f7f\u7528\u5546\u7528DNA\u63d0\u53d6\u8bd5\u5242\u76d2\u7b49\u3002\u63d0\u53d6\u8fc7\u7a0b\u4e2d\u8981\u786e\u4fddDNA\u7684\u5b8c\u6574\u6027\u548c\u7eaf\u5ea6\u3002<br>3\u3001\u6ce8\u610f\u4e8b\u9879\uff1a\u6839\u636e\u5b9e\u9a8c\u9700\u6c42\uff0c\u9009\u62e9\u5408\u9002\u7684DNA\u6d53\u5ea6\u4f5c\u4e3aPCR\u7684\u6a21\u677f\u3002\u6d53\u5ea6\u8fc7\u9ad8\u53ef\u80fd\u4f1a\u6291\u5236PCR\u53cd\u5e94\uff0c\u800c\u6d53\u5ea6\u8fc7\u4f4e\u5219\u53ef\u80fd\u5bfc\u81f4\u6269\u589e\u6548\u679c\u4e0d\u4f73\u3002<\/p>\n\n\n\n<p><strong>\u4e8c\u3001PCR\u53cd\u5e94\u4f53\u7cfb\u7684\u914d\u5236<\/strong><br>1\u3001\u7ec4\u6210\u6210\u5206\uff1a<br>\u6a21\u677fDNA\uff1a\u63d0\u4f9b\u5f85\u6269\u589e\u7684DNA\u5e8f\u5217\u3002<br>\u5f15\u7269\uff1a\u4e0e\u5f85\u6269\u589e\u7684DNA\u7247\u6bb5\u4e24\u7aef\u5206\u522b\u4e92\u8865\u7684\u77ed\u94feDNA\u5206\u5b50\uff0c\u7528\u4e8e\u5f15\u5bfcDNA\u805a\u5408\u9176\u5408\u6210\u65b0\u7684DNA\u94fe\u3002<br>dNTPs\uff1a\u8131\u6c27\u6838\u82f7\u9178\uff0c\u5305\u62ecA\u3001T\u3001C\u3001G\u56db\u79cd\uff0c\u662fDNA\u5408\u6210\u7684\u539f\u6599\u3002<br>\u7f13\u51b2\u6db2\uff1a\u63d0\u4f9b\u5408\u9002\u7684pH\u503c\u548c\u79bb\u5b50\u73af\u5883\uff0c\u7ef4\u6301PCR\u53cd\u5e94\u7684\u6b63\u5e38\u8fdb\u884c\u3002<br>\u805a\u5408\u9176\uff1a\u5982Taq DNA\u805a\u5408\u9176\uff0c\u5177\u6709\u8010\u70ed\u6027\uff0c\u80fd\u5728\u9ad8\u6e29\u4e0b\u4fdd\u6301\u6d3b\u6027\uff0c\u50ac\u5316DNA\u94fe\u7684\u5408\u6210\u3002<br>2\u3001\u914d\u5236\u65b9\u6cd5\uff1a\u6309\u7167\u5b9e\u9a8c\u8981\u6c42\uff0c\u5c06\u4e0a\u8ff0\u6210\u5206\u6309\u4e00\u5b9a\u6bd4\u4f8b\u6df7\u5408\uff0c\u5f62\u6210PCR\u53cd\u5e94\u4f53\u7cfb\u3002<\/p>\n\n\n\n<p><strong>\u4e09\u3001PCR\u5faa\u73af\u53cd\u5e94\u7684\u8bbe\u7f6e<\/strong><br>1\u3001\u57fa\u672c\u6b65\u9aa4\uff1a<br>\u53d8\u6027\uff1a\u5c06PCR\u53cd\u5e94\u6df7\u5408\u6db2\u52a0\u70ed\u81f395\u00b0C\u5de6\u53f3\uff0c\u4f7f\u6a21\u677fDNA\u7684\u53cc\u94fe\u89e3\u5f00\uff0c\u5f97\u5230\u5355\u94feDNA\u3002<br>\u9000\u706b\uff08\u5f15\u7269\u7ed3\u5408\uff09\uff1a\u5c06\u53cd\u5e94\u4f53\u7cfb\u964d\u6e29\u81f3\u9002\u5b9c\u6e29\u5ea6\uff08\u901a\u5e38\u4e3a45~65\u00b0C\uff09\uff0c\u4f7f\u5f15\u7269\u4e0e\u5355\u94feDNA\u7684\u4e92\u8865\u533a\u57df\u7ed3\u5408\u3002<br>\u5ef6\u4f38\uff1a\u5c06\u53cd\u5e94\u4f53\u7cfb\u5347\u6e29\u81f3\u805a\u5408\u9176\u7684\u6700\u9002\u6e29\u5ea6\uff08\u901a\u5e38\u4e3a65~75\u00b0C\uff09\uff0c\u805a\u5408\u9176\u5728\u5f15\u7269\u7684\u57fa\u7840\u4e0a\u5408\u6210\u65b0\u7684DNA\u94fe\u3002<br>2\u3001\u5faa\u73af\u6b21\u6570\uff1aPCR\u7684\u5faa\u73af\u6b21\u6570\u53d6\u51b3\u4e8e\u5f85\u6269\u589e\u7684DNA\u7247\u6bb5\u7684\u521d\u59cb\u6d53\u5ea6\u548cPCR\u7684\u653e\u5927\u6548\u7387\u3002\u901a\u5e38\u8fdb\u884c25~35\u4e2aPCR\u5faa\u73af\u3002<\/p>\n\n\n\n<p><strong>\u56db\u3001PCR\u4ea7\u7269\u7684\u5206\u6790<\/strong><br>1\u3001\u65b9\u6cd5\uff1a<br>\u51dd\u80f6\u7535\u6cf3\uff1a\u5e38\u7528\u7684\u51dd\u80f6\u6709\u743c\u8102\u7cd6\u51dd\u80f6\u548c\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u3002\u901a\u8fc7\u51dd\u80f6\u7535\u6cf3\u53ef\u4ee5\u5206\u79bb\u548c\u68c0\u6d4bPCR\u4ea7\u7269\uff0c\u6839\u636e\u4ea7\u7269\u7684\u8fc1\u79fb\u8ddd\u79bb\u548c\u5e26\u72b6\u56fe\u53ef\u4ee5\u5224\u65adPCR\u4ea7\u7269\u7684\u957f\u5ea6\u548c\u6269\u589e\u6548\u7387\u3002<br>\u5176\u4ed6\u65b9\u6cd5\uff1a\u5982\u8367\u5149\u5b9a\u91cfPCR\u3001\u6d4b\u5e8f\u7b49\uff0c\u4e5f\u53ef\u4ee5\u7528\u4e8e\u5206\u6790PCR\u4ea7\u7269\u7684\u7279\u5f02\u6027\u548c\u6d53\u5ea6\u3002<br>2\u3001\u7ed3\u679c\u89e3\u8bfb\uff1a<br>\u6269\u589e\u6548\u7387\uff1a\u6839\u636ePCR\u4ea7\u7269\u7684\u5e26\u72b6\u56fe\u53ef\u4ee5\u5224\u65ad\u6269\u589e\u6548\u7387\u7684\u597d\u574f\u3002<br>\u6269\u589e\u7279\u5f02\u6027\uff1a\u6269\u589e\u51fa\u7684PCR\u4ea7\u7269\u5e94\u53ea\u6709\u5355\u4e00\u7684\u76ee\u6807\u5e8f\u5217\uff0c\u65e0\u975e\u7279\u5f02\u6027\u6269\u589e\u3002<br>\u6269\u589e\u957f\u5ea6\uff1a\u901a\u8fc7\u51dd\u80f6\u7535\u6cf3\u53ef\u4ee5\u786e\u5b9aPCR\u4ea7\u7269\u7684\u957f\u5ea6\u662f\u5426\u7b26\u5408\u9884\u671f\u3002<br>\u7eaf\u5ea6\u4e0e\u6d53\u5ea6\uff1a\u53ef\u4ee5\u901a\u8fc7\u6d4b\u5b9aA260\/A280\u7684\u6bd4\u503c\u6765\u8bc4\u4f30PCR\u4ea7\u7269\u7684\u7eaf\u5ea6\uff0c\u5e76\u901a\u8fc7\u6bd4\u8f83PCR\u4ea7\u7269\u4e0eDNA\u5927\u5c0f\u6807\u51c6\u54c1\u7684\u5bc6\u5ea6\u6765\u4f30\u7b97\u5176\u6d53\u5ea6\u3002<\/p>\n\n\n\n<p>\u5728\u6df1\u5165\u5256\u6790\u5e76\u8be6\u7ec6\u9610\u8ff0\u4e86PCR\u4f53\u7cfb\u6784\u5efa\u7684\u56db\u5927\u6838\u5fc3\u6b65\u9aa4\u540e\uff0c\u6211\u4eec\u4e0d\u96be\u53d1\u73b0\uff0c\u8fd9\u4e00\u8fc7\u7a0b\u7684\u6bcf\u4e00\u6b65\u90fd\u51dd\u805a\u4e86\u79d1\u5b66\u7814\u7a76\u7684\u4e25\u8c28\u4e0e\u6280\u672f\u7684\u7cbe\u5999\u3002\u4ece\u6a21\u677fDNA\u7684\u7cbe\u786e\u63d0\u53d6\uff0c\u5230\u53cd\u5e94\u4f53\u7cfb\u7684\u7ec6\u81f4\u914d\u5236\uff0c\u518d\u5230\u5faa\u73af\u53c2\u6570\u7684\u7cbe\u786e\u8c03\u63a7\uff0c\u76f4\u81f3\u6700\u7ec8\u4ea7\u7269\u7684\u4e25\u683c\u9274\u5b9a\uff0cPCR\u4f53\u7cfb\u6784\u5efa\u7684\u6bcf\u4e00\u6b65\u90fd\u5982\u540c\u7cbe\u5bc6\u7684\u9f7f\u8f6e\uff0c\u76f8\u4e92\u54ac\u5408\uff0c\u5171\u540c\u9a71\u52a8\u7740\u8fd9\u4e00\u5f3a\u5927\u751f\u7269\u6280\u672f\u7684\u8fd0\u8f6c\u3002<\/p>\n\n\n\n<p>\u76f8\u5173\u9605\u8bfb\u63a8\u8350<\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/quick-start-guide-for\/\">\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\u5feb\u901f\u5165\u95e8\u6307\u5357<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/magnetic-module\/\">\u9002\u914d\u5668\u78c1\u94c1\u652f\u6301\u6587\u7ae0<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/knowledge2\/3d-print\/\">3D\u6253\u5370\u76ee\u5f55<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/opentrons-flex-rouxinglajicao\/\">Opentrons Flex \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\u7684\u67d4\u6027\u5783\u573e\u69fd<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/ot-2-ngs-gongzuozhanpeizhishebei\/\">OT-2 NGS\u5de5\u4f5c\u7ad9\u9700\u8981\u914d\u7f6e\u54ea\u4e9b\u8bbe\u5907<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/opentrons.com.cn\/news\/opentrons-flex-gongzuozhanhuanjingyaoqiu\/\">Opentrons Flex \u6838\u9178\u63d0\u53d6\u5de5\u4f5c\u7ad9\u5bf9\u5b9e\u9a8c\u5ba4\u73af\u5883\u7684\u8981\u6c42<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u4e00\u79cd\u5e7f\u6cdb\u5e94\u7528\u7684\u6280\u672f\uff0c\u7528\u4e8e\u6269\u589e\u7279\u5b9a\u7684DNA\u5e8f\u5217\u3002\u65e0\u8bba\u662f\u57fa\u56e0\u514b\u9686\u3001\u7a81\u53d8\u5206\u6790\uff0c [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":8618,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-8616","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>PCR\u4f53\u7cfb\u6784\u5efa\u7684\u56db\u4e2a\u6b65\u9aa4 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/news\/pcr-tixigoujian\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR\u4f53\u7cfb\u6784\u5efa\u7684\u56db\u4e2a\u6b65\u9aa4 - Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"og:description\" content=\"PCR\uff08\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff09\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u4e00\u79cd\u5e7f\u6cdb\u5e94\u7528\u7684\u6280\u672f\uff0c\u7528\u4e8e\u6269\u589e\u7279\u5b9a\u7684DNA\u5e8f\u5217\u3002\u65e0\u8bba\u662f\u57fa\u56e0\u514b\u9686\u3001\u7a81\u53d8\u5206\u6790\uff0c [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/opentrons.com.cn\/news\/pcr-tixigoujian\/\" \/>\n<meta property=\"og:site_name\" content=\"Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"article:published_time\" content=\"2025-02-19T07:38:57+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-02-19T07:39:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2025\/02\/image-23.png\" \/>\n\t<meta property=\"og:image:width\" content=\"553\" \/>\n\t<meta property=\"og:image:height\" content=\"312\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Huang, Chris\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"Huang, Chris\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 \u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-tixigoujian\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/opentrons.com.cn\/news\/pcr-tixigoujian\/\"},\"author\":{\"name\":\"Huang, 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