{"id":516,"date":"2023-02-22T13:00:00","date_gmt":"2023-02-22T05:00:00","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=case&#038;p=516"},"modified":"2023-12-10T23:29:35","modified_gmt":"2023-12-10T15:29:35","slug":"retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2","status":"publish","type":"case","link":"https:\/\/zh-tw.opentrons.com.cn\/resources\/customer-stories\/retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2\/","title":{"rendered":"Retro Biosciences\u4f7f\u7528OT-2\u81ea\u52d5\u5316\u55ae\u7d30\u80deRNA\u5b9a\u5e8f\u6587\u5eab\u88fd\u5099"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>\u55ae\u7d30\u80de RNA \u5b9a\u5e8f\u80fd\u5920\u4ee5\u55ae\u7d30\u80de\u70ba\u55ae\u4f4d\u9032\u884c\u8f49\u9304\u7d44\u5b78\u5206\u6790\uff0c\u9019\u9805\u7814\u7a76\u53ef\u4ee5\u8b93\u6211\u5011\u9032\u4e00\u6b65\u4e86\u89e3\u4e00\u4e9b\u7a00\u6709\u4e14\u8cb4\u91cd\u7684\u7d30\u80de\u7fa4\u3002\u5118\u7ba1\u55ae\u7d30\u80deRNA\u5b9a\u5e8f\u6280\u8853\u7684\u4f7f\u7528\u8d8a\u4f86\u8d8a\u5ee3\u6cdb\uff0c\u4f46\u9019\u9805\u6280\u8853\u7684\u6210\u672c\u4ecd\u7136\u76f8\u7576\u6602\u8cb4\u3002\u9664\u4e86\u5b9a\u5e8f\u5e73\u53f0\u548c\u6bcf\u6b21\u55ae\u7368\u904b\u884c\u7684\u6210\u672c\u5916\uff0c\u6587\u5eab\u88fd\u5099\u6b65\u9a5f\u65e2\u6602\u8cb4\u53c8\u8cbb\u529b\u3002\u7136\u800c\uff0c\u628a\u6587\u5eab\u88fd\u5099\u6b65\u9a5f\u9032\u884c\u81ea\u52d5\u5316\u53ef\u4ee5\u6709\u6548\u6e1b\u5c11\u5be6\u9a57\u7d50\u679c\u7684\u8b8a\u7570\u6027\uff0c\u540c\u6642\u7bc0\u7701\u7814\u7a76\u4eba\u54e1\u7684\u5bf6\u8cb4\u6642\u9593\uff0c\u89e3\u653e\u4ed6\u5011\u7684\u96d9\u624b\uff0c\u8b93\u79d1\u5b78\u7814\u7a76\u4eba\u54e1\u80fd\u6709\u66f4\u591a\u6642\u9593\u7528\u4f86\u8a2d\u8a08\u5be6\u9a57\u6216\u5206\u6790\u6578\u64da\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u76ee\u524d\uff0c\u5e02\u9762\u4e0a\u80fd\u5be6\u73fe\u55ae\u7d30\u80deRNA\u5b9a\u5e8f\u7684\u81ea\u52d5\u5316\u6587\u5eab\u88fd\u5099\u6210\u672c\u9ad8\u9054 26\u842c\u7f8e\u5143\uff0c\u6602\u8cb4\u7684\u6210\u672c\u5df2\u7d93\u8d85\u51fa\u4e86\u5e02\u9762\u4e0a\u5927\u90e8\u5206\u5c0f\u578b\u7814\u7a76\u5be6\u9a57\u5ba4\u7684\u9810\u7b97\u3002\u70ba\u4e86\u5411\u66f4\u591a\u5be6\u9a57\u5ba4\u63d0\u4f9b\u9019\u9805\u81ea\u52d5\u5316\u6280\u8853\uff0cRetro Biosciences \u6210\u529f\u300c\u7834\u89e3\u300d\u4e86 Opentrons \u7684 OT-2 \u81ea\u52d5\u5316\u79fb\u6db2\u5e73\u53f0\uff0c\u4f7f\u5176\u80fd\u5920\u5be6\u73fe\u81ea\u52d5\u5316\u6587\u5eab\u88fd\u5099\u529f\u80fd\uff0c\u540c\u6642\u6210\u672c\u50c5\u70ba 2.6\u842c\u7f8e\u5143\uff01\u7bc0\u7d04\u4e8610\u500d\u7684\u555f\u52d5\u6210\u672c\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>Retro Biosciences<\/strong>&nbsp;\u662f\u4e00\u5bb6\u4f4d\u65bc\u52a0\u5dde\u7684\u751f\u7269\u91ab\u5b78\u516c\u53f8\uff0c\u5c08\u6ce8\u65bc\u8840\u6f3f\u7642\u6cd5\u3001\u7d30\u80de\u91cd\u7de8\u7a0b\u548c\u7d30\u80de\u81ea\u566c\u7814\u7a76\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2381,\"width\":\"490px\"} -->\n<figure class=\"wp-block-image is-resized\"><img src=\"https:\/\/opentrons.com\/wp-content\/uploads\/2023\/02\/retro-bio-OT-2-768x1024.jpeg\" alt=\"Retro Bio's OT-2, hacked to perform scRNAseq at a fraction of the cost\" class=\"wp-image-2381\" style=\"width:490px\" title=\"Retro Bio's OT-2, hacked to perform scRNAseq at a fraction of the cost\"><figcaption class=\"wp-element-caption\">Retro Bio \u4f7f\u7528OT-2 \u9032\u884c\u55ae\u7d30\u80de\u5b9a\u5e8f\u6587\u5eab\u88fd\u5099\uff08\u5716\u7247\u7531 Retro Biosciences \u7684 Ben \u63d0\u4f9b\uff09<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p><strong>Q: \u8acb\u7c21\u55ae\u4ecb\u7d39\u4e00\u4e0bRetro Biosciences \u516c\u53f8\uff1f <\/strong>A: Retro Biosciences \u7684\u6210\u7acb\u6700\u521d\u662f\u4e00\u5bb6\u81e8\u5e8a\u6cbb\u7642\u85e5\u7269\u7814\u767c\u516c\u53f8\uff0c\u5f8c\u4f86\u4e3b\u8981\u696d\u52d9\u662f\u958b\u767c\u9810\u9632\u6027\u85e5\u7269\u4f86\u89e3\u6c7a\u7d30\u80de\u8001\u5316\u554f\u984c\u3002\u5927\u591a\u6578\u7814\u7a76\u8001\u5316\u7684\u751f\u7269\u5b78\u516c\u53f8\u90fd\u5c08\u6ce8\u65bc\u55ae\u4e00\u7684\u8870\u8001\u6a5f\u5236\uff0c\u800c Retro Biosciences \u662f\u4e00\u5bb6\u591a\u5c08\u6848\u516c\u53f8\uff0c\u6211\u5011\u52aa\u529b\u63a2\u7d22\u7684\u4e09\u7a2e\u7642\u6cd5\u6709\uff1a\u7d30\u80de\u91cd\u7de8\u7a0b\u3001\u81ea\u566c\u548c\u8840\u6f3f\u555f\u767c\u7642\u6cd5\u3002\u6211\u5011\u4e86\u89e3\u5230\u91ab\u9662\u63a5\u8a3a\u7684\u75c5\u4eba\u6709 80% \u4ee5\u4e0a\u7684\u75be\u75c5\u8ca0\u64d4\u6e90\u81ea\u65bc\u8001\u5316\u6a5f\u5236\uff0c\u56e0\u6b64\u6211\u5011\u5e0c\u671b\u627e\u5230\u80fd\u76f4\u63a5\u89e3\u6c7a\u9019\u4e9b\u554f\u984c\u7684\u7642\u6cd5\uff0c\u5e6b\u52a9\u66f4\u591a\u75c5\u4eba\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>Q:<\/strong>&nbsp;<strong>\u81ea\u52d5\u5316\u5982\u4f55\u5e6b\u52a9\u60a8\u9054\u6210\u76ee\u6a19\uff1f <\/strong>A: \u6a5f\u5668\u81ea\u52d5\u5316\u662f\u4e0d\u77e5\u75b2\u5026\u7684\uff0c\u53ef\u4ee5\u6301\u7e8c\u6027\u5de5\u4f5c\uff0c\u540c\u6642\u81ea\u52d5\u5316\u7684\u6548\u7387\u986f\u8457\u8d85\u8d8a\u4eba\u5de5\u3002\u6211\u5011\u6b63\u5728\u63a2\u7d22\u672a\u77e5\u7684\u751f\u7269\u5b78\uff0c\u5176\u4e2d\u4e00\u4e9b\u63a2\u7d22\u6d89\u53ca\u5230\u7684\u53c3\u6578\u4ee5\u53ca\u53c3\u6578\u7d44\u5408\u975e\u5e38\u5ee3\u6cdb\uff0c\u5982\u679c\u7e7c\u200b\u200b\u7e8c\u8b93\u79d1\u5b78\u7814\u7a76\u4eba\u54e1\u4f7f\u7528\u624b\u52d5\u7684\u65b9\u5f0f\u9032\u884c\u5be6\u9a57\uff0c\u6211\u8a8d\u70ba\u9019\u662f\u5c0d\u7814\u7a76\u8005\u79d1\u5b78\u982d\u8166\u7684\u6d6a\u8cbb\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>Q: \u60a8\u70ba\u81ea\u52d5\u5316\u55ae\u7d30\u80deRNA\u5b9a\u5e8f\u7684\u6587\u5eab\u88fd\u5099\u5275\u5efa\u4e86\u4e00\u500b\u975e\u5e38\u9177\u4e14\u975e\u5e38\u5be6\u60e0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u8acb\u544a\u8a34\u6211\u5011\u60a8\u5275\u9020\u9019\u500b\u65b9\u6848\u7684\u521d\u8877\uff0c\u4ee5\u53ca\u60a8\u662f\u5982\u4f55\u505a\u5230\u7684\uff1f <\/strong>A: \u6587\u5eab\u88fd\u5099\u5c0d\u6211\u5011\u4f86\u8aaa\u662f\u4e00\u500b\u5165\u9580\u9805\u76ee\u3002\u81ea\u52d5\u5316\u7684\u6700\u5927\u512a\u52e2\u662f\u4f60\u53ef\u4ee5\u5c07\u90a3\u4e9b\u56fa\u6709\u7684\u3001\u5927\u91cf\u91cd\u8907\u7684\u3001\u6700\u4e0d\u5e0c\u671b\u51fa\u932f\u7684\u74b0\u7bc0\u81ea\u52d5\u904b\u4f5c\u8d77\u4f86\uff0c\u7bc0\u7701\u6642\u9593\uff0c\u6e1b\u5c11\u4eba\u5de5\u5931\u8aa4\u3002\u6211\u5011\u5728\u9019\u88e1\u505a\u4e86\u5f88\u591a\u55ae\u7d30\u80deRNA\u5b9a\u5e8f\u3002\u6bcf\u4e00\u6b21\u6e2c\u5e8f\u6211\u5011\u9700\u8981\u9762\u81e8\u7684\u5206\u6790\u548c\u7814\u7a76\u4efb\u52d9\u90fd\u5f88\u91cd\uff0c\u56e0\u6b64\u6211\u5011\u5fc5\u9808\u958b\u767c\u65b0\u7684\u8edf\u9ad4\u529f\u80fd\uff08\u4f8b\u5982\u6db2\u4f4d\u7279\u5b9a\u79fb\u6db2\uff09\u548c\u786c\u9ad4\u529f\u80fd\uff08\u4f8b\u5982\u6539\u9032\u7684\u78c1\u73e0\u5206\u96e2\u7cfb\u7d71\uff09\uff0c\u5b83\u4f7f\u6211\u5011\u80fd\u5920\u76f4\u89c0\u5730\u5be9\u67e5\u5354\u8b70\u4e2d\u7684\u6240\u6709\u6b65\u9a5f\uff0c\u4ee5\u767c\u73fe\u932f\u8aa4\u4e26\u9a57\u8b49\u54c1\u8cea\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>Q: <strong>\u60a8\u5728<strong><a href=\"https:\/\/github.com\/retrobiosciences\/opentrons-10x\" target=\"_blank\" rel=\"noreferrer noopener\">Github<\/a>&nbsp;&lt; \/strong&gt;\u4e0a\u767c\u8868\u4e86\u9019\u9805\u5de5\u4f5c\uff0c\u5f15\u767c\u4e86\u4e0d\u932f\u7684\u53cd\u97ff\uff0c \u60a8\u70ba\u4ec0\u9ebc\u6c7a\u5b9a\u516c\u958b\u9019\u9805\u5de5\u4f5c\uff1f <\/strong><\/strong>A:\u5c0d\u6211\u5011\u4f86\u8aaa\uff0c\u5728\u5ee3\u6cdb\u7684\u751f\u7269\u5b78\u7814\u7a76\u4e2d\u964d\u4f4e\u79d1\u7814\u6210\u672c\u5c0d\u793e\u6703\u7684\u597d\u8655\uff0c\u9060\u9060\u8d85\u904e\u4e86\u6211\u5011\u5c0d\u540c\u884c\u7684\u7af6\u722d\u95dc\u4fc2\u7684\u72f9\u9698\u64d4\u6182\u3002\u6211\u5011\u5e0c\u671b\u6709\u66f4\u591a\u5f9e\u4e8b\u540c\u985e\u7814\u7a76\u7684\u79d1\u5b78\u7814\u7a76\u8005\u53ef\u4ee5\u5f9e\u81ea\u52d5\u5316\u4e2d\u7372\u76ca\u3002<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>Retro Biosciences \u900f\u904e\u5c55\u793aOpentrons\u958b\u6e90\u81ea\u52d5\u5316\u79fb\u6db2\u7cfb\u7d71\u5275\u9020\u4e86\u5c6c\u65bc\u81ea\u5df1\u7684\u55ae\u7d30\u80de\u5b9a\u5e8f\u6587\u5eab\u88fd\u5099\u81ea\u52d5\u5316\u6d41\u7a0b\uff0c\u5e6b\u52a9\u4e86\u66f4\u591a\u7814\u7a76\u55ae\u7d30\u80de\u5b9a\u5e8f\u7684\u5be6\u9a57\u5ba4\u80fd\u5920\u555f\u52d5\u81ea\u52d5\u5316\uff0c\u63d0\u5347\u4ed6\u5011\u7684\u7814\u7a76\u6548\u7387\u3002\u6211\u5011\u7684\u958b\u6e90\u81ea\u52d5\u5316\u7a0b\u5f0f\u53ef\u4ee5\u900f\u904e\u7a0b\u5f0f\u8a2d\u8a08\u4f86\u5b8c\u6210\u7814\u7a76\u4eba\u54e1\u9700\u8981\u7684\u4efb\u4f55\u4e8b\u60c5\uff0c\u540c\u6642\u628a\u6210\u672c\u63a7\u5236\u5728\u8f03\u4f4e\u7684\u7bc4\u570d\u5167\u3002\u6211\u5011\u88ab Retro Biosciences \u5f15\u9818\u7684\u5275\u65b0\u6240\u9707\u64bc\uff0c\u671f\u5f85\u4ed6\u5011\u672a\u4f86\u7684\u6301\u7e8c\u5275\u65b0\u3002<\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>\u55ae\u7d30\u80de RNA \u5b9a\u5e8f\u80fd\u5920\u4ee5\u55ae\u7d30\u80de\u70ba\u55ae\u4f4d\u9032\u884c\u8f49\u9304\u7d44\u5b78\u5206\u6790\uff0c\u9019\u9805\u7814\u7a76\u53ef\u4ee5\u8b93\u6211\u5011\u9032\u4e00\u6b65\u4e86\u89e3\u4e00\u4e9b\u7a00\u6709\u4e14\u8cb4\u91cd\u7684\u7d30\u80de\u7fa4\u3002<\/p>\n","protected":false},"featured_media":517,"template":"","class_list":["post-516","case","type-case","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Retro Biosciences\u4f7f\u7528OT-2\u81ea\u52a8\u5316\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6587\u5e93\u5236\u5907 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zh-tw.opentrons.com.cn\/case\/retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Retro Biosciences\u4f7f\u7528OT-2\u81ea\u52a8\u5316\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6587\u5e93\u5236\u5907 - Opentrons\u4e2d\u56fd\u5b98\u7f51\" \/>\n<meta property=\"og:description\" content=\"\u55ae\u7d30\u80de RNA \u5b9a\u5e8f\u80fd\u5920\u4ee5\u55ae\u7d30\u80de\u70ba\u55ae\u4f4d\u9032\u884c\u8f49\u9304\u7d44\u5b78\u5206\u6790\uff0c\u9019\u9805\u7814\u7a76\u53ef\u4ee5\u8b93\u6211\u5011\u9032\u4e00\u6b65\u4e86\u89e3\u4e00\u4e9b\u7a00\u6709\u4e14\u8cb4\u91cd\u7684\u7d30\u80de\u7fa4\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/opentrons.com.cn\/case\/retro-biosciences-automates-single-cell-rnaseq-library-prep-using-the-ot-2\/\" \/>\n<meta property=\"og:site_name\" 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