{"id":557,"date":"2020-01-07T14:06:00","date_gmt":"2020-01-07T06:06:00","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=case&p=557"},"modified":"2023-12-29T16:58:26","modified_gmt":"2023-12-29T08:58:26","slug":"assembling-synthetic-dna-easily-and-cheaply-at-imperial-college","status":"publish","type":"case","link":"https:\/\/opentrons.com.cn\/zh-TW\/resources\/customer-stories\/assembling-synthetic-dna-easily-and-cheaply-at-imperial-college\/","title":{"rendered":"\u5e1d\u570b\u5b78\u9662\u4f7f\u7528 OT-2 \u5efa\u69cb\u81ea\u52d5\u5316DNA\u7d44\u88dd\u5e73\u53f0"},"content":{"rendered":"\n

\u5982\u4f55\u5229\u7528BASIC\u548cOpentrons OT-2\u5efa\u69cb\u4e86\u4e00\u500b\u7528\u65bc\u5408\u6210\u751f\u7269\u5b78\u7684\u6e96\u78ba\u3001\u9ad8\u6027\u50f9\u6bd4\u3001\u81ea\u52d5\u5316\u7684DNA\u7d44\u88dd\u5e73\u53f0\u3002<\/h2>\n\n\n\n

\u70ba\u4e86\u66f4\u6df1\u5165\u3001\u66f4\u5feb\u901f\u5730\u63a2\u7d22\u751f\u7269\u5b78\uff0c\u79d1\u5b78\u5bb6\u9700\u8981\u7c21\u55ae\u6709\u6548\u7684\u65b9\u6cd5\u4f86\u5efa\u69cb DNA \u7d50\u69cb\u3002<\/p>\n\n\n\n

\u300c\u70ba\u4e86\u66f4\u6709\u6548\u5730\u958b\u767c\u65b0\u7684\u751f\u7269\u7cfb\u7d71\uff0c\u6211\u5011\u6b63\u5728\u5617\u8a66\u5c07\u5de5\u7a0b\u65b9\u6cd5\u61c9\u7528\u65bc\u751f\u7269\u5b78\u3002\u300d\u5e1d\u570b\u7406\u5de5\u5b78\u9662\u5408\u6210\u751f\u7269\u5b78\u4e2d\u5fc3<\/a>\u7684\u5171\u540c\u4e3b\u4efbGeoff Baldwin<\/a> \u8aaa\uff1a\u300c\u5f9e\u904e\u5f80\u7684\u7d93\u9a57\u4f86\u770b\uff0c\u88fd\u9020\u65b0\u7684DNA\u7d50\u69cb\u662f\u500b\u6f2b\u9577\u7684\u904e\u7a0b\uff0c\u901a\u5e38\u9700\u8981\u5169\u5230\u4e09\u500b\u6708\u7684\u6642\u9593\uff0d\u800c\u4e14\u4e2d\u9014\u6709\u53ef\u80fd\u5931\u6557\u3002 \u4ee5\u53ca\u502b\u6566\u751f\u7269\u9444\u9020\u5ee0<\/a>\u81ea\u52d5\u5316\u4e3b\u7ba1\u535a\u58eb\u5f8c\u7814\u7a76\u54e1 Matt Haines <\/a>\u4e00\u8d77\u958b\u767c\u4e00\u500b\u53ef\u4ee5\u66f4\u8f15\u9b06\u3001\u66f4\u5feb\u901f\u69cb\u5efaDNA\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\uff08\u5f9e\u5de6\u5230\u53f3\uff09Matt Haines\u548cGeoff Baldwin\u5728\u4ed6\u5011\u7684\u5be6\u9a57\u5ba4\u88e1\uff0c\u8207OT-2\u6db2\u9ad4\u8655\u7406\u5be6\u9a57\u5ba4\u6a5f\u5668\u4eba\u5728\u4e00\u8d77\u3002\u5716\u7247\u4f86\u6e90\uff1a\u5e1d\u570b\u5b78\u9662<\/p>\n\n\n\n

\u6211\u5011\u5df2\u7d93\u505a\u4e86\u4e00\u4e9b\u5de5\u5177\u4e0a\u7684\u6539\u9032\u3002 \u201c\u4eca\u5929\uff0c\u6211\u5011\u6709\u66f4\u597d\u7684\u5de5\u5177\uff0c\u201dBaldwin\u8aaa\uff0c\u201c\u5c31\u50cf\u4f60\u5efa\u9020\u5b9c\u5bb6\u5bb6\u5177\u7684\u65b9\u5f0f\u4e00\u6a23\uff0c\u4f60\u53ef\u4ee5\u7528\u6a19\u6e96\u96f6\u4ef6\u5efa\u9020\u4e00\u500b\u751f\u7269\u7cfb\u7d71\uff0c\u9019\u662f\u4e00\u7a2e\u66f4\u6709\u6548\u7684\u65b9\u5f0f\u3002\u201d<\/p>\n\n\n\n

\n

\u201c\u5c31\u50cf\u4f60\u5efa\u9020\u5b9c\u5bb6\u5bb6\u5177\u7684\u65b9\u5f0f\u4e00\u6a23\uff0c\u4f60\u53ef\u4ee5\u7528\u6a19\u6e96\u96f6\u4ef6\u5efa\u9020\u4e00\u500b\u751f\u7269\u7cfb\u7d71\uff0c\u9019\u662f\u4e00\u7a2e\u66f4\u6709\u6548\u7684\u65b9\u5f0f\u3002\u201d<\/p>\n<\/blockquote>\n\n\n\n

\u9032\u5165\u57fa\u790e\u77e5\u8b58<\/strong>\u5728\u958b\u555f\u81ea\u52d5\u5316\u5c08\u6848\u4e4b\u524d\uff0cStorch\u3001Baldwin \u548c\u5176\u4ed6\u540c\u4e8b\u958b\u767c\u4e86 BASIC\uff0c\u4e3b\u8981\u7528\u65bc\u51aa\u7b49\u514b\u9686\u7684\u751f\u7269\u90e8\u4ef6\u7d44\u88dd\u6a19\u6e96\u5316\u3002\u5728\u67d0\u7a2e\u7a0b\u5ea6\u4e0a\uff0cBASIC \u4fc3\u4f7f\u7814\u7a76\u4eba\u54e1\u4ee5\u65b0\u7684\u65b9\u5f0f\u601d\u8003\u5efa\u69cb DNA \u7d50\u69cb\u3002 \u201c\u5b83\u63d0\u4f9b\u4e86\u51fa\u8272\u7684\u53ef\u64cd\u4f5c\u6027\uff0c\u201dBaldwin\u8aaa\uff1a\u201c\u4e00\u65e6\u4f60\u63a5\u53d7\u4e86\u6a19\u6e96\u5316\u65b9\u6cd5\uff0c\u5c31\u6703\u6709\u5927\u7a0b\u5ea6\u7684\u601d\u8003\u81ea\u7531\uff0c\u4f60\u4e0d\u518d\u9700\u8981\u8003\u616e\u55ae\u500b\u5e8f\u5217\u3001\u91cd\u758a\u3001PCR \u7b49\u7684\u7d30\u7bc0\u3002\u201d<\/p>\n\n\n\n

\u5728\u67d0\u7a2e\u7a0b\u5ea6\u4e0a\uff0cBASIC \u4fc3\u4f7f\u7814\u7a76\u4eba\u54e1\u4ee5\u65b0\u7684\u65b9\u5f0f\u601d\u8003\u5efa\u69cbDNA\u69cb\u5efa\u9ad4\u3002 \u300c\u5b83\u63d0\u4f9b\u4e86\u6975\u597d\u7684\u53ef\u64cd\u4f5c\u6027\uff0c\u300dBaldwin\u8aaa\u3002 \u201c\u4e00\u65e6\u4f60\u63a5\u53d7\u4e86\u6a19\u6e96\u5316\u7684\u65b9\u6cd5\uff0c\u5c31\u6703\u6709\u66f4\u5927\u7684\u81ea\u8003\u81ea\u7531\u5ea6\u2014\u2014\u4f60\u4e0d\u518d\u9700\u8981\u8003\u616e\u55ae\u5e8f\u5217\u3001\u91cd\u758a\u3001PCR\u7b49\u7684\u7d30\u7bc0\u3002\u201d<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u900f\u904e BASIC\uff0c\u79d1\u5b78\u5bb6\u5c07\u6ce8\u610f\u529b\u5f9e\u5982\u4f55\u5efa\u69cb DNA \u8f49\u79fb\u5230\u4e86\u601d\u8003\u7cfb\u7d71\u7684\u884c\u70ba\u3002\u6b64\u5916\uff0cBASIC \u7522\u751f\u7684DNA \u5efa\u69cb\u975e\u5e38\u7cbe\u78ba\u3002 \u300c\u4f60\u6709\u4fe1\u5fc3\uff0c\u5408\u6210\u7684 DNA\u5c31\u662f\u4f60\u60f3\u8981\u7684\uff0c\u300dBaldwin\u8aaa\u3002<\/p>\n\n\n\n

\n

\u900f\u904e BASIC\uff0c\u79d1\u5b78\u5bb6\u5c07\u6ce8\u610f\u529b\u5f9e\u5982\u4f55\u5efa\u69cb DNA \u8f49\u79fb\u5230\u4e86\u601d\u8003\u7cfb\u7d71\u7684\u884c\u70ba\u4e0a\uff0c\u540c\u6642\u7522\u751f\u975e\u5e38\u7cbe\u78ba\u7684DNA\u5efa\u69cb\u3002<\/p>\n<\/blockquote>\n\n\n\n

\u9664\u4e86\u63d0\u9ad8\u6e96\u78ba\u5ea6\u5916\uff0cBASIC \u9084\u7c21\u5316\u4e86\u5efa\u69cb DNA \u7684\u904e\u7a0b\u3002 \u300c\u5b83\u63d0\u4f9b\u4e86\u66f4\u7c21\u55ae\u7684\u65b9\u6cd5\u4f86\u5be6\u73fe DNA \u69cb\u5efa\uff0c\u300dStorch\u8aaa\u3002 \u300c\u4f60\u5f97\u5230\u7684\u662f\u958b\u7bb1\u5373\u7528\u7684\u96f6\u4ef6\u548c\u9023\u63a5\u5668\uff0c\u800c\u4e14\u4e0d\u9700\u8981\u9032\u884c\u6545\u969c\u6392\u9664\u3002\u300d\u7136\u5f8c\uff0c\u79d1\u5b78\u5bb6\u53ea\u9700\u8981\u77e5\u9053\u5982\u4f55\u5c07\u90e8\u5206-\u9023\u63a5\u5668-\u90e8\u5206-\u9023\u63a5\u5668\u7b49\u9032\u884c\u7d44\u5408\u4f86\u5efa\u9020\u4e00\u500b\u7d50\u69cb\u3002<\/p>\n\n\n\n

Baldwin \u5c07\u5176\u8207\u96fb\u8166\u7684\u6f14\u5316<\/a>\u9032\u884c\u6bd4\u8f03\u3002\u5e7e\u5341\u5e74\u524d\uff0c\u5efa\u9020\u96fb\u8166\u7684\u4eba\u9700\u8981\u4e86\u89e3\u6676\u9ad4\u7ba1\u548c\u908f\u8f2f\u9598\u3002 \u300c\u5982\u4eca\uff0c\u60a8\u4e0d\u5fc5\u64d4\u5fc3\u8a08\u7b97\u7684\u7269\u7406\u539f\u7406\uff0c\u56e0\u70ba\u60a8\u53ef\u4ee5\u76f4\u63a5\u5f9e\u76d2\u5b50\u88e1\u62ff\u51fa\u7d44\u4ef6\uff0c\u300d\u4ed6\u8aaa\u3002 \u201c\u540c\u6a23\uff0cBASIC \u8b93\u7528\u6236\u80fd\u5920\u4eab\u53d7\u8207DNA \u985e\u4f3c\u7684\u62bd\u8c61\u5c64\u6b21\u3002\u201d<\/p>\n\n\n\n

\u4e0d\u904e\uff0c\u60f3\u8981\u5145\u5206\u5229\u7528 BASIC\uff0c\u9084\u9700\u8981\u5be6\u9a57\u5ba4\u81ea\u52d5\u5316\u3002<\/p>\n\n\n\n

\u6dfb\u52a0Opentrons<\/strong>\u300c\u7576Opentrons \u63a8\u51faOT-2\u6642\uff0cOpentrons\u7684\u958b\u6e90\u7406\u5ff5\u78ba\u5be6\u8207\u6211\u5011\u5c0d\u57fa\u65bc\u9023\u7d50\u5668\u7684DNA \u7d44\u88dd\u5c08\u6848\u7684\u770b\u6cd5\u4e0d\u8b00\u800c\u5408\uff0c\u300dStorch \u8aaa\uff1a\u300c\u78c1\u6027\u6a21\u7d44\u548c\u6eab\u63a7\u6a21\u7d44\u63d0\u4f9b\u4e86\u6211\u5011\u9700\u8981\u7684\u529f\u80fd\u3002<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u958b\u6e90BASIC \u6280\u8853\uff08\u53ef\u5728GitHub \u4e0a\u514d\u8cbb\u7372\u5f97<\/a>\uff09\u975e\u5e38\u9069\u5408\u5728\u958b\u6e90\u7684\u81ea\u52d5\u5316\u79fb\u6db2\u6a5f\u5668\u4ebaOT-2\u4e0a\u4f7f\u7528\u3002 \u300c\u6211\u5011\u76f8\u4fe1\u5be6\u9a57\u7684\u50f9\u503c\u61c9\u8a72\u9ad4\u73fe\u5728\u4f60\u5efa\u9020\u7684\u6771\u897f\u4e0a\uff0c\u800c\u4e0d\u662f\u4f60\u5efa\u9020\u5b83\u7684\u65b9\u5f0f\u4e0a\u300dBaldwin\u8aaa\u3002\u9019\u50ac\u751f\u4e86\u80fd\u5920\u904b\u884c BASIC \u7684 OT-2\u2014\u2014DNA-BOT\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\uff08\u5f9e\u5de6\u5230\u53f3\uff09Matt Haines\u548cGeoff Baldwin\u6b63\u5728\u601d\u8003\u4ed6\u5011\u7684DNA-Bot\u3002\u5716\u7247\u4f86\u6e90\uff1a\u5e1d\u570b\u5b78\u9662<\/p>\n\n\n\n

\u7576\u88ab\u554f\u53ca\u904b\u884c DNA-BOT \u7684\u512a\u52e2\u6642\uff0cHaines \u8aaa\uff0c\u300c\u9996\u5148\u662f\u6027\u50f9\u6bd4\u3002\u300d \u7136\u5f8c\uff0c\u4ed6\u4e5f\u5f37\u8abf\u4e86\u8a72\u65b9\u6cd5\u7684\u6548\u7387\u548c\u6e96\u78ba\u6027\u3002 \u201c\u6211\u5011\u5728\u6bcf\u500b\u6848\u4f8b\u4e2d\u90fd\u7372\u5f97\u4e86 88 \u500b\u7d44\u4ef6\u7684\u69cb\u9020\uff0c\u518d\u9ad8\u6548\u7684\u540c\u6642\u5be6\u73fe\u4e86\u9ad8\u7cbe\u5ea6\uff0c\u201d\u4ed6\u8aaa\uff1a\u201c\u9019\u662f\u975e\u6a19\u6e96\u5316\u7684\u65b9\u5f0f\u96e3\u4ee5\u5be6\u73fe\u7684\u3002\u201d<\/p>\n\n\n\n

\u5728\u5e1d\u570b\u5b78\u9662\u5408\u6210\u751f\u7269\u5b78\u4e2d\u5fc3\uff0cBaldwin \u548c\u4ed6\u7684\u540c\u4e8b\u5efa\u7acb\u4e86\u4e00\u500b\u7531\u516d\u500b DNA-BOT \u7d44\u6210\u7684\u5be6\u9a57\u5ba4\u3002 \u201c\u6211\u5011\u6b63\u5728\u5617\u8a66\u770b\u770b\u78a9\u58eb\u751f\u662f\u5426\u53ef\u4ee5\u4f7f\u7528\u9019\u500b\u5e73\u53f0\u69cb\u5efa\u7d50\u69cb\uff0c\u201dStorch \u8aaa\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u7814\u7a76\u751f\u6b63\u5728\u8a2d\u7f6e\u5e1d\u570b\u5b78\u9662\u751f\u7269\u9444\u9020\u5ee0\u3002\u5716\u7247\u4f86\u6e90\uff1a\u502b\u6566\u5e1d\u570b\u5b78\u9662<\/em><\/p>\n\n\n\n

\u7121\u8ad6\u9019\u4e9b\u5b78\u751f\u7684\u8a66\u9a57\u7d50\u679c\u5982\u4f55\uff0cBaldwin \u7684\u5718\u968a\u5df2\u7d93\u77e5\u9053 DNA-BOT \u6539\u8b8a\u4e86\u904a\u6232\u898f\u5247\u3002 \u201c\u6211\u5011\u53ef\u4ee5\u63a2\u7d22\u7684\u8a2d\u8a08\u7a7a\u9593\u662f\u6578\u767e\u500b\uff0c\u751a\u81f3\u6578\u5343\u500bDNA\u7d50\u69cb\uff0c\u201dBaldwin \u8aaa\uff0c\u201c\u800c\u4e14\u5b83\u53ef\u4ee5\u8fc5\u901f\u589e\u52a0\u5230\u6578\u842c\u500b\u3002\u201d<\/p>\n\n\n\n

\u6b63\u5982 Haines \u6240\u5f37\u8abf\u7684\uff0c\u201c\u9084\u6709\u5f88\u591a\u53ef\u63a2\u7d22\u7684\u8b8a\u7570\u6027\u3002\u201d<\/p>\n\n\n\n

\u589e\u5f37\u61c9\u7528\u7a0b\u5f0f<\/strong>Baldwin \u7684\u5718\u968a\u770b\u5230\u4e86\u66f4\u591a\u7684\u5de5\u4f5c\u8981\u505a\u3002\u4ed6\u5011\u5df2\u7d93\u770b\u5230 DNA-BOT \u5728\u5404\u7a2e\u61c9\u7528\u4e2d\u767c\u63ee\u4f5c\u7528\uff0c\u5305\u62ec\u7814\u7a76\u73fe\u6709\u7684\u751f\u7269\u5408\u6210\u9014\u5f91\u548c\u5275\u5efa\u65b0\u7684\u9014\u5f91\u4f86\u5efa\u9020\u96e3\u4ee5\u88fd\u9020\u7684\u5316\u5408\u7269\u3002<\/p>\n\n\n\n

\u8a72\u5718\u968a\u5c07\u7e7c\u7e8c\u958b\u767c\u548c\u8abf\u6574 DNA-BOT\uff0c\u4e26\u8a08\u5283\u6574\u5408 Opentrons Thermocycler\u3002 \u201c\u6211\u5011\u60f3\u5efa\u7acb\u4e00\u500b\u751f\u614b\u7cfb\u7d71\uff0c\u201dBaldwin \u8aaa\u3002 \u300c\u5275\u5efa\u4e00\u500bDNA \u96f6\u4ef6\u548c\u6d41\u7a0b\uff0c\u5c07\u589e\u5f37\u5718\u968a\u6210\u54e1\u7684\u80fd\u529b\u3002\u300d\u7576\u50f9\u683c\u5be6\u60e0\u3001\u6613\u65bc\u4f7f\u7528\u548c\u9ad8\u6548\u7684\u81ea\u52d5\u5316\u8207\u5148\u9032\u7684\u5408\u6210\u751f\u7269\u5b78\u5de5\u5177\u7bb1\u76f8\u7d50\u5408\u6642\uff0c\u69cb\u5efaDNA \u7d50\u69cb\u7684\u6574\u500b\u884c\u696d\u5c07\u767c\u751f\u8b8a\u5316\uff0c\u5e7e\u4e4e\u4efb\u4f55\u4eba\u90fd\u53ef\u4ee5\u63a2\u7d22\u5b83\u3002<\/p>\n\n\n\n

\u53c3\u8003\u8cc7\u6599\uff1a<\/strong><\/p>\n\n\n\n

    \n
  1. Storch, M., Haines, M.C., Baldwin, G.S. 2019. DNA-BOT: \u5408\u6210\u751f\u7269\u5b66\u4e2d\u4f4e\u6210\u672c\u3001\u81ea\u52a8\u5316\u7684DNA\u7ec4\u88c5\u5e73\u53f0\u3002bioRxiv. doi: HTTP:\/\/\u6771\u897f.do i.org\/10.1101\/832139<\/a>.<\/li>\n\n\n\n
  2. Storch, M., Casini, A., Mackrow, B., et al. 2015. BASIC: \u7528\u65bc\u5408\u6210\u751f\u7269\u5b78\u7684\u540c\u614b\u514b\u9686\u7684\u65b0\u7684\u751f\u7269\u90e8\u4ef6\u7d44\u88dd\u6a19\u6e96\uff0c\u70ba\u5408\u6210\u751f\u7269\u5b78\u63d0\u4f9b\u6e96\u78ba\u3001\u55ae\u5c64DNA\u7d44\u88dd\uff0c ACS Synth. Biol. 4:781\u2013787.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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