{"id":2751,"date":"2024-05-27T14:40:56","date_gmt":"2024-05-27T06:40:56","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2751"},"modified":"2024-09-03T17:48:49","modified_gmt":"2024-09-03T09:48:49","slug":"opentrons-flex","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/opentrons-flex\/","title":{"rendered":"Opentrons Flex \u81ea\u52d5\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad924 \u5c0f\u6642\u5168\u7a0b\u81ea\u52d5\u5316\u8f15\u9b06\u88fd\u5099\u9ad8\u9054 384 \u500b\u8cea\u7c92\u6587\u5eab"},"content":{"rendered":"<!-- wp:paragraph {\"style\":{\"typography\":{\"fontSize\":\"16px\"}}} -->\n<p style=\"font-size:16px\"><strong>\u7c21\u4ecb<\/strong>\u9ad8\u901a\u91cf\u57fa\u56e0\u7d44\u5206\u6790\u662f\u751f\u547d\u79d1\u5b78\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u91cd\u8981\u5de5\u5177\uff0c\u9019\u9805\u6280\u8853\u5728\u63a8\u52d5\u79d1\u7814\u9032\u6b65\u65b9\u9762\u767c\u63ee\u8457\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u70ba\u4e86\u6eff\u8db3\u4e86\u79d1\u5b78\u7814\u7a76\u9818\u57df\u5c0d\u9ad8\u6548\u3001\u6e96\u78ba\u88fd\u5099\u65b9\u6cd5\u7684\u8feb\u5207\u9700\u6c42\uff0cseqWell \u7684 Express Plex\u2122Library Kit \u63d0\u4f9b\u4e86\u4e00\u7a2e\u5c08\u9580\u7528\u65bc\u9ad8\u901a\u91cf\u88fd\u5099\u8cea\u7c92\u548c\u64f4\u589e\u5b50\u6587\u5eab\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u5728\u50b3\u7d71\u7684\u624b\u5de5\u88fd\u5099\u6587\u5eab\u65b9\u6cd5\u4e2d\uff0c\u5b8c\u6210 96 \u500b\u6a23\u54c1\u7684\u88fd\u5099\u9700\u8981\u82b1\u8cbb\u9577\u9054 90 \u5206\u9418\u7684\u6642\u9593\uff0c\u5176\u4e2d\u624b\u52d5\u64cd\u4f5c\u6642\u9593\u4f54\u64da\u4e86 30 \u5206\u9418\u3002\u9019\u7a2e\u65b9\u5f0f\u4e0d\u50c5\u6548\u7387\u4f4e\u4e0b\uff0c\u9084\u5bb9\u6613\u56e0\u70ba\u4eba\u70ba\u56e0\u7d20\u800c\u5f15\u5165\u8aa4\u5dee\u3002\u70ba\u4e86\u6539\u5584\u6b64\u4e00\u72c0\u6cc1\uff0c\u672c\u6587\u6bd4\u8f03\u4e86\u5169\u7a2e\u6587\u5eab\u88fd\u5099\u65b9\u6cd5\uff0c\u4e00\u7a2e\u662f\u5728 Opentrons Flex\u2122 \u79fb\u6db2\u5de5\u4f5c\u7ad9\u4e0a\u4f7f\u7528seqWell ExpressPlex\u2122 Library Kit \uff0c\u53e6\u4e00\u7a2e\u662f\u624b\u5de5\u88fd\u5099\u6587\u5eab\u3002\u900f\u904e\u7d50\u5408 Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u8207 seqWell ExpressPlex\u2122 Library Kit\uff0c\u6211\u5011\u53ef\u4ee5\u5927\u5e45\u7e2e\u77ed\u6587\u5eab\u88fd\u5099\u6642\u9593\uff0c\u63d0\u9ad8\u6a23\u672c\u901a\u91cf\u3002\u9019\u7a2e\u81ea\u52d5\u5316\u7684\u89e3\u6c7a\u65b9\u6848\u4e0d\u50c5\u63d0\u5347\u4e86\u88fd\u5099\u6548\u7387\uff0c\u4e5f\u964d\u4f4e\u4e86\u4eba\u70ba\u8aa4\u5dee\u7684\u98a8\u96aa\uff0c\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u63d0\u4f9b\u4e86\u66f4\u53ef\u9760\u3001\u66f4\u6709\u6548\u7387\u7684\u5de5\u5177\u3002 <strong>\u6750\u8cea\u8207\u65b9\u6cd5<\/strong>ExpressPlex \u5de5\u4f5c\u6d41\u7a0b:\u6839\u64da Express Plex \u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u6211\u5011\u5728 Flex \u81ea\u52d5\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad9\u4e0a\u9032\u884c\u6587\u5eab\u88fd\u5099\u3002\u5be6\u9a57\u904e\u7a0b\u4e2d\uff0c\u6211\u5011\u8f38\u516516 ng \u7684 pUC19 \uff0c\u518d\u5c07\u6240\u6709\u7d44\u5206\u52a0\u5165\u53cd\u61c9\u677f \uff0c \u7528 3000 rpm \u7684\u8f49\u901f\u6df7\u52fb 90 \u79d2\uff0c\u7136\u5f8c\u9032\u884c\u5f8c\u7e8c\u7684\u6587\u5eab\u88fd\u5099\u548c\u6df7\u5408\u6b65\u9a5f\u3002\u6700\u7d42\u7684\u6587\u5eab\u5728 NextSeq 2000 \u5b9a\u5e8f\u5100\u4e0a\u9032\u884c 2X150 bp \u5b9a\u5e8f\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2569,\"sizeSlug\":\"large\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-large\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/\u65e0\u6807\u9898-623x1024.png\" alt=\"ExpressPlex \u5de5\u4f5c\u6d41\u7a0b\" class=\"wp-image-2569\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u57161.A ) Express Plex \u5de5\u4f5c\u6d41\u7a0b\u5305\u62ec\u5c07index \u548cDNA \u52a0\u5165\u53cd\u61c9\u677f\uff08\u5df2\u542b\u6709\u9175\u7d20\u7b49\u6df7\u5408\u7269\uff09\uff0c\u7136\u5f8c\u9032\u884c\u64f4\u589e\u3001\u6df7\u5408\u548cMAGwise \u78c1\u73e0\u7d14\u5316\uff1bB ) Flex \u5de5\u4f5c\u7ad9\u5305\u62ec\u5438\u982d\u76d2\u3001\u6a21\u7d44\u3001\u8f49\u677f\u6293\u624b\u548c\u5176\u4ed6\u914d\u4ef6\uff1bC ) Flex \u5de5\u4f5c\u7ad9\u677f\u4f4d\u8a2d\u7f6e\uff0c\u7528\u65bc\u5728\u64f4\u589e\u5f8c\u5c074 \u500b\u53cd\u61c9\u677f\u9032\u884c\u6587\u5eab\u6df7\u5408\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u7d50\u679c<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2570,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/0012.jpg\" alt=\"Opentrons Flex\u663e\u793a\u7ed3\u679c\" class=\"wp-image-2570\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u57162 . A ) \u7d50\u679c\u986f\u793a96 \u5b54\u677f\u4e2d\u6587\u5eab\u6578\u64da\u8b8a\u7570\u4fc2\u6578( CV )\u70ba12.7%\uff1b B ) 95% \u7684\u6587\u5eab\u6578\u64da\u901a\u904e\u4e86\u6578\u64da\u9810\u8655\u7406\uff0c\u70ba\u9ad8\u8cea\u91cf\u6578\u64da\uff0c\u4e14\u8d85\u904e90% \u6587\u5eab\u8cea\u91cf\u5f97\u5206\u9054\u5230Q30\uff1b C ) \u7531Flex \u88fd\u5099\u7684\u6587\u5eab\u6bd4\u624b\u52d5\u88fd\u5099\u7684\u6587\u5eab\u6fc3\u5ea6\u66f4\u9ad8\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2571,\"width\":\"840px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/00120240513201156.jpg\" alt=\"\u72ec\u7acb\u7684 96 \u6df7 1 \u6587\u5e93\" class=\"wp-image-2571\" style=\"width:840px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5716 3 . \u5229\u7528\u932f\u958b\u7684\u958b\u59cb\u6642\u9593\uff0c\u53ef\u4ee5\u5728 Opentrons FlexTM \u4e0a 3 \u5c0f\u6642\u5167\u88fd\u5099 8 \u500b\u7368\u7acb\u7684 96 \u6df7 1 \u6587\u5eab\u3002\u5728 Flex \u4e0a\u8a2d\u5b9a\u7b2c\u4e00\u584a\u53cd\u61c9\u677f\u5f8c\uff0c\u53ef\u4ee5\u5728 24 \u5c0f\u6642\u5167\u5b8c\u6210 768 \u500b\u6587\u5eab\u7684\u88fd\u5099\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u7d50\u8ad6<\/strong>\u5728 Flex \u79fb\u6db2\u5e73\u53f0\u4e0a\u88fd\u5099\u7684 96 \u500b pUC19 \u8cea\u7c92\u6a23\u672c\u6587\u5eab\u5448\u73fe\u51fa\u4e86\u9ad8\u5ea6\u5747\u4e00\u7684\u8cea\u91cf\uff0c\u5145\u5206\u8b49\u660e\u4e86 Flex\u5e73\u53f0\u5728\u81ea\u52d5\u5316\u6587\u5eab\u88fd\u5099\u65b9\u9762\u7684\u5353\u8d8a\u6027\u80fd\u3002\u8207\u624b\u52d5\u88fd\u5099\u76f8\u6bd4\uff0c\u81ea\u52d5\u5316\u6587\u5eab\u88fd\u5099\u4e0d\u50c5\u7a69\u5b9a\u6027\u8f03\u4f73\uff0c\u800c\u4e14\u6587\u5eab\u6fc3\u5ea6\u66f4\u9ad8\u3002\u6b64\u5916\uff0c\u8d85\u904e95%\u7684\u8b80\u53d6\u6210\u529f\u901a\u904e\u5b9a\u5e8f\u5100\u8cea\u6aa2 PF\uff0c\u9019\u9032\u4e00\u6b65\u9a57\u8b49\u4e86\u81ea\u52d5\u5316\u88fd\u5099\u6d41\u7a0b\u7684\u9ad8\u6548\u7387\u548c\u6e96\u78ba\u6027\u3002 Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u652f\u63f4\u5728\u55ae\u6b21\u904b\u884c\u4e2d\u8655\u7406\u591a\u9054 384 \u500b\u6a23\u672c\uff0c\u5927\u5927\u63d0\u9ad8\u4e86\u6a23\u672c\u8655\u7406\u6548\u7387\u3002\u900f\u904e\u5408\u7406\u5b89\u6392\u6642\u9593\uff0c\u6211\u5011\u751a\u81f3\u53ef\u4ee5\u5728 24 \u5c0f\u6642\u5167\u8655\u7406\u9ad8\u9054 768 \u500b\u6a23\u672c\uff0c\u5f9e\u800c\u6eff\u8db3\u5927\u898f\u6a21\u9ad8\u901a\u91cf\u57fa\u56e0\u7d44\u5206\u6790\u7684\u9700\u6c42\u3002\u5728 Flex \u5e73\u53f0\u4e0a\u81ea\u52d5\u5316 ExpressPlex\u2122 Library Prep Kit \u5de5\u4f5c\u6d41\u7a0b\uff0c\u6211\u5011\u5be6\u73fe\u4e86\u5f9e\u63d0\u53d6\u7684\u8cea\u7c92\/\u64f4\u589e\u5b50 DNA \u5230\u8cc7\u6599\u751f\u6210\u7684\u5168\u6d41\u7a0b\u81ea\u52d5\u5316\u3002\u9019\u500b\u89e3\u6c7a\u65b9\u6848\u4e0d\u50c5\u7c21\u5316\u4e86\u64cd\u4f5c\u6b65\u9a5f\uff0c\u964d\u4f4e\u4e86\u4eba\u70ba\u8aa4\u5dee\uff0c\u800c\u4e14\u986f\u8457\u63d0\u9ad8\u4e86\u5be6\u9a57\u6548\u7387\u548c\u6578\u64da\u8cea\u91cf\uff0c\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u9818\u57df\u7684\u767c\u5c55\u6ce8\u5165\u4e86\u65b0\u7684\u52d5\u529b\u3002<\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>\u7c21\u4ecb\u9ad8\u901a\u91cf\u57fa\u56e0\u7d44\u5206\u6790\u662f\u751f\u547d\u79d1\u5b78\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u91cd\u8981\u5de5\u5177\uff0c\u9019\u9805\u6280\u8853\u5728\u63a8\u52d5\u79d1\u7814\u9032\u6b65\u65b9\u9762\u767c\u63ee\u8457\u81f3\u95dc\u91cd\u8981\u7684\u4f5c\u7528\u3002\u70ba\u4e86\u6eff [&hellip;]<\/p>\n","protected":false},"featured_media":2568,"template":"","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"knowledge2_cat":[96],"knowledge2_app":[],"class_list":["post-2751","knowledge2","type-knowledge2","status-publish","has-post-thumbnail","hentry","knowledge2_cat-biji"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - 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