{"id":2752,"date":"2024-05-27T14:47:01","date_gmt":"2024-05-27T06:47:01","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2752"},"modified":"2024-09-03T17:47:36","modified_gmt":"2024-09-03T09:47:36","slug":"opentrons-elisa","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/opentrons-elisa\/","title":{"rendered":"\u4f7f\u7528 Opentrons \u79fb\u6db2\u5de5\u4f5c\u7ad9\u9032\u884c\u81ea\u52d5\u5316Elisa\uff08\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u8a66\u9a57\uff09"},"content":{"rendered":"<!-- wp:paragraph -->\n<p><strong>\u7c21\u4ecb<\/strong>ELISA\uff08\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u5be6\u9a57\uff09\u662f\u4e00\u7a2e\u5ee3\u6cdb\u61c9\u7528\u65bc\u8a3a\u65b7\u548c\u7814\u7a76\u9818\u57df\u7684\u91cd\u8981\u6e2c\u8a66\u65b9\u6cd5\u3002\u5728\u9019\u9805\u6280\u8853\u4e2d\uff0c\u78ba\u4fdd\u6a23\u672c\u8655\u7406\u7684\u4e00\u81f4\u6027\u81f3\u95dc\u91cd\u8981\uff0c\u800c\u6e1b\u5c11\u4eba\u5de5\u64cd\u4f5c\u6642\u9593\u5247\u53ef\u4ee5\u6709\u6548\u964d\u4f4e\u4eba\u70ba\u8aa4\u5dee\u3002\u6211\u5011\u5229\u7528Opentrons OT-2 \u548c Flex \u6db2\u9ad4\u8655\u7406\u5de5\u4f5c\u7ad9\uff0c\u958b\u767c\u4e26\u6e2c\u8a66\u4e86\u591a\u7a2e\u81ea\u52d5\u5316\u5354\u5b9a\u3002\u900f\u904e\u9019\u4e9b\u81ea\u52d5\u5316\u5354\u8b70\uff0c\u60a8\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528 ELISA \u8a66\u5291\u76d2\u9032\u884c\u5be6\u9a57\uff0c\u4e5f\u53ef\u4ee5\u6839\u64da\u9700\u8981\u81ea\u8a02\u7279\u5b9a\u7684\u5be6\u9a57\u6d41\u7a0b\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u65b9\u6cd5<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2575,\"sizeSlug\":\"full\",\"linkDestination\":\"none\",\"align\":\"right\",\"className\":\"is-style-rounded\"} -->\n<figure class=\"wp-block-image alignright size-full is-style-rounded\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/01_20240513202314.jpg\" alt=\"\u68c0\u6d4b\u65b9\u6cd5\u793a\u610f\u56fe\" class=\"wp-image-2575\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph {\"align\":\"left\"} -->\n<p class=\"has-text-align-left\">\u8a66\u5291\u76d2: Takara Fibronectin (FN) EIA Kit\uff08\u593e\u5fc3\u6cd5ELISA\uff0c\u57161\uff09<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li>\u5100\u5668: Opentrons OT-2<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u5de5\u4f5c\u6d41\u7a0b:<\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list -->\n\n<!-- wp:list {\"ordered\":true} -->\n<ol><!-- wp:list-item -->\n<li>\u52a0\u6a23\uff08\u9776\u5411\u6355\u7372\uff09<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6d17\u6ecc<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u52a0\u9176\u6a19\u6297\u9ad4<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6d17\u6ecc<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u52a0\u986f\u8272\u5e95\u7269<\/li>\n<!-- \/wp:list-item --><\/ol>\n<!-- \/wp:list -->\n\n<!-- wp:group {\"layout\":{\"type\":\"constrained\"}} -->\n<div class=\"wp-block-group\"><!-- wp:paragraph -->\n<p>\u6aa2\u6e2c\u65b9\u6cd5: Tecan Cortisol Saliva ELISA Kit\uff08\u7af6\u722d\u6cd5ELISA\uff0c\u57162\uff09<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2576,\"width\":\"363px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\",\"align\":\"right\",\"className\":\"is-style-default\"} -->\n<figure class=\"wp-block-image alignright size-full is-resized is-style-default\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/02_20240513202331.jpg\" alt=\"\u68c0\u6d4b\u65b9\u6cd5\u793a\u610f\u56fe\" class=\"wp-image-2576\" style=\"width:363px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li>\u5100\u5668: Opentrons OT-2<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u5de5\u4f5c\u6d41\u7a0b:<\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list -->\n\n<!-- wp:list {\"ordered\":true} -->\n<ol><!-- wp:list-item -->\n<li>\u52a0\u6a23\uff08\u9776\u5411\u6355\u7372\uff09&amp;\u6a19\u8a18\u6297\u539f<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6d17\u6ecc<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u52a0\u986f\u8272\u5e95\u7269<\/li>\n<!-- \/wp:list-item --><\/ol>\n<!-- \/wp:list --><\/div>\n<!-- \/wp:group -->\n\n<!-- wp:paragraph -->\n<p><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li>\u68c0\u6d4b\u65b9\u6cd5: Cell Sciences SARS-CoV-2 Surrogate Virus Neutralization Test Kit<br>\uff08\u7ade\u4e89\u6cd5ELISA\uff0c\u56fe3\uff09<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u5100\u5668: Opentrons OT-2<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u5de5\u4f5c\u6d41\u7a0b:<\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list -->\n\n<!-- wp:list {\"ordered\":true} -->\n<ol><!-- wp:list-item -->\n<li>\u52a0\u6a23\uff08\u9776\u5411\u6355\u7372\uff09 &amp; \u6a19\u8a18ACE2<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6d17\u6ecc<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u52a0\u986f\u8272\u5e95\u7269<\/li>\n<!-- \/wp:list-item --><\/ol>\n<!-- \/wp:list -->\n\n<!-- wp:image {\"id\":2577,\"sizeSlug\":\"full\",\"linkDestination\":\"none\",\"align\":\"right\"} -->\n<figure class=\"wp-block-image alignright size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/03_20240513202343.jpg\" alt=\"\u68c0\u6d4b\u65b9\u6cd5\u793a\u610f\u56fe\" class=\"wp-image-2577\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li>\u68c0\u6d4b\u65b9\u6cd5: SARS-CoV-2 Spike RBD-coupled Magnetic Beads<br>(\u78c1\u73e0\u5f0fELISA, \u56fe4)<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u5100\u5668: Opentrons OT-2 &amp; \u78c1\u529b\u67b6\u6a21\u7d44<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u5de5\u4f5c\u6d41\u7a0b:<\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list -->\n\n<!-- wp:list {\"ordered\":true} -->\n<ol><!-- wp:list-item -->\n<li>\u52a0\u6a23\uff08\u9776\u5411\u6355\u7372\uff09<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6d17\u6ecc<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u52a0\u9176\u6a19\u6297\u9ad4<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6d17\u6ecc<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u52a0\u986f\u8272\u5e95\u7269<\/li>\n<!-- \/wp:list-item --><\/ol>\n<!-- \/wp:list -->\n\n<!-- wp:image {\"id\":2578,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/04_20240513202356.jpg\" alt=\"\u68c0\u6d4b\u65b9\u6cd5\u793a\u610f\u56fe\" class=\"wp-image-2578\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":2579,\"width\":\"398px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\",\"align\":\"right\"} -->\n<figure class=\"wp-block-image alignright size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/05_20240513203637.jpg\" alt=\"ELISA \u677f\u8bbe\u8ba1\" class=\"wp-image-2579\" style=\"width:398px;height:auto\"><figcaption class=\"wp-element-caption\">\u57165<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u6aa2\u6e2c\uff1a\u7d30\u80de\u6fc0\u7d20\u6aa2\u6e2c\u7684\u6e2c\u5b9a\u65b9\u6cd5\u958b\u767c\uff08\u593e\u5fc3\u6cd5ELISA\uff0c\u57165 \uff09\u5100\u5668: Opentrons Flex\u5de5\u4f5c\u6d41\u7a0b:1 .ELISA \u677f\u6e96\u50992.\u6d17\u6ecc3.\u52a0\u6a23\uff08\u9776\u5411\u6355\u7372\uff094.\u6d17\u6ecc5.\u52a0\u9176\u6a19\u6297\u9ad46.\u6d17\u6ecc7.\u52a0\u5e95\u7269\u986f\u8272<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u7d50\u679c<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2580,\"width\":\"715px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/06_20240513203828.jpg\" alt=\"\u7ed3\u679c\u663e\u793a\u56fe\" class=\"wp-image-2580\" style=\"width:715px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5716 6. Takara Fibronectin (FN) EIA Kit\uff08\u5716 1 \uff09\u3002\u5c0dFN\u6a23\u54c1\u9032\u884c\u68af\u5ea6\u7a00\u91cb\u4e26\u6aa2\u9a57\uff0c\u6aa2\u9a57\u7d50\u679c\u5982\u5716\u6240\u793a:\u5de6\u5716\u70baFN\u6a23\u54c1\u6fc3\u5ea6\u548c\u5e73\u5747\u5438\u5149\u5ea6\uff08n=3\uff09\u6578\u64da\uff0c\u5448\u73fe\u9ad8\u5ea6\u7dda\u6027\u8ff4\u6b78\uff1b\u53f3\u5716\u70ba3\u6b21\u91cd\u8907\u5be6\u9a57\u7d50\u679c\uff0c\u5448\u73fe\u826f\u597d\u7684\u91cd\u8907\u6027\u548c\u7a69\u5b9a\u6027\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2581,\"width\":\"757px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/07_20240513203941.jpg\" alt=\"\u7ed3\u679c\u663e\u793a\u56fe\" class=\"wp-image-2581\" style=\"width:757px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5716 7. Tecan Cortisol Saliva ELISA Kit \uff08\u5716 2 \uff09\u3002\u76ae\u8cea\u9187\u6a23\u672c\u9032\u884c\u68af\u5ea6\u7a00\u91cb\u4e26\u6aa2\u9a57\uff0c\u6aa2\u9a57\u7d50\u679c\u5982\u5716\u6240\u793a\uff1a\u5de6\u5716\u70ba\u76ae\u8cea\u9187\u6a23\u672c\u6fc3\u5ea6\u8207\u5e73\u5747\u5438\u5149\u5ea6\uff08n=3\uff09\u6578\u64da\uff0c\u5448\u73fe\u9ad8\u5ea6\u7dda\u6027\u8ff4\u6b78\uff1b\u53f3\u5716\u70ba3\u6b21\u91cd\u8907\u5be6\u9a57\u7d50\u679c\uff0c\u5448\u73fe\u826f\u597d\u7684\u91cd\u8907\u6027\u548c\u7a69\u5b9a\u6027\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2582,\"width\":\"772px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/08_20240513204159.jpg\" alt=\"\" class=\"wp-image-2582\" style=\"width:772px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5716 8. Cell Sciences SARS-CoV-2 Surrogate\uff08\u5716 3 \uff09\u3002\u6aa2\u5b9a\u7d50\u679c\u8868\u660e\uff0c\u967d\u6027\u7684\u4eba\u8840\u6e05\u6a23\u672c\uff08\u5148\u524d\u5df2\u901a\u904eFDA \u8a8d\u8b49\u7684ELISA \u5be6\u9a57\u6aa2\u6e2c\u7d50\u679c\u70ba\u967d\u6027\uff09\uff0c\u5728\u8a72\u6e2c\u8a66\u4e2d\u5448\u73fe\u7d50\u679c\u70ba\u967d\u6027\uff08\u6291\u5236\u7387&gt;20%\uff0c\u8a08\u7b97\u516c\u5f0f\uff1a\u6291\u5236\u7387= [1 - (\u6a23\u672c\u7684OD450\u503c\/ \u9670\u6027\u5c0d\u7167\u7684OD\u503c)] x 100% \uff0cn = 3\uff09\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2583,\"width\":\"712px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/09_20240513204235.jpg\" alt=\"\" class=\"wp-image-2583\" style=\"width:712px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5716 9. SARS-CoV-2 Spike RBD-coupled Magnetic Beads\uff08\u5716 4 \uff09\u3002\u5c0d\u91cd\u7d44\u4e2d\u548c\u6297\u9ad4\u6a23\u672c\u9032\u884c\u68af\u5ea6\u7a00\u91cb\u4e26\u6aa2\u9a57\u3002\u6aa2\u5b9a\u7d50\u679c\u8868\u660e\uff0c\u967d\u6027\u7684\u4eba\u8840\u6e05\u6a23\u672c\uff08\u5148\u524d\u5df2\u901a\u904e FDA \u8a8d\u8b49\u7684 ELISA \u5be6\u9a57\u6aa2\u6e2c\u7d50\u679c\u70ba\u967d\u6027\uff09\uff0c\u5728\u8a72\u6e2c\u8a66\u4e2d\u5448\u73fe\u7d50\u679c\u70ba\u967d\u6027\uff08n = 3\uff09\uff08\u53f3\uff09\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2584,\"width\":\"700px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/10_20240513204314.jpg\" alt=\"\" class=\"wp-image-2584\" style=\"width:700px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5716 10. Assay Development for Cytokine Detection\uff08\u5716 5 \uff09\u3002\u5728 96 \u5b54\u677f\u5857\u8986\u6297 TNF-alpha\u6297\u9ad4\u548c\u6297IFN-gamma\u6297\u9ad4\uff0c\u5c0d\u542b\u6709\u7d30\u80de\u6fc0\u7d20\u7684\u6a23\u672c\u9032\u884cELISA\u6aa2\u6e2c\uff0c\u6578\u64da\u5982\u5716\u6240\u793a\uff08n=6\uff09\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u7d50\u8ad6<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li>\u6b64\u5de5\u4f5c\u7ad9\u53ef\u7528\u65bc\u57f7\u884c\u5f9e\u52a0\u6297\u9ad4\/\u6297\u539f\u5230\u52a0\u986f\u8272\u5e95\u7269\u7684\u5b8c\u6574 ELISA \u6d41\u7a0b<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u7ed3\u679c\u8868\u660e\u6837\u672c\u5904\u7406\u7684\u4e00\u81f4\u6027\u9ad8\uff08\u53d8\u5f02\u7cfb\u6570&lt;10%\uff09\u3001\u51c6\u786e\u6027\u9ad8\uff08R-squared&gt;0.99\uff09\uff0c\u91cd\u590d\u6027<br>\u597d\u3002<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u4ee5 SARS-CoV-2 \u6297\u4f53\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u57fa\u4e8e 96 \u5b54\u677f\u7684\u5546\u7528\u8bd5\u5242\u548c\u57fa\u4e8e\u78c1\u73e0\u7684\u81ea\u5236 ELISA \u8bd5\u5242\u5747\u53ef<br>\u5b9e\u73b0\u5168\u81ea\u52a8\u5316\u5904\u7406\uff0c\u4e14\u7ed3\u679c\u4e0e FDA \u8ba4\u8bc1\u7684 Elisa \u65b9\u6cd5\u6d4b\u5b9a\u7ed3\u679c\u4e00\u81f4\u3002<\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list -->","protected":false},"excerpt":{"rendered":"<p>\u7c21\u4ecbELISA\uff08\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u5be6\u9a57\uff09\u662f\u4e00\u7a2e\u5ee3\u6cdb\u61c9\u7528\u65bc\u8a3a\u65b7\u548c\u7814\u7a76\u9818\u57df\u7684\u91cd\u8981\u6e2c\u8a66\u65b9\u6cd5\u3002\u5728\u9019\u9805\u6280\u8853\u4e2d\uff0c\u78ba\u4fdd\u6a23\u672c\u8655\u7406 [&hellip;]<\/p>\n","protected":false},"featured_media":2570,"template":"","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"knowledge2_cat":[96],"knowledge2_app":[],"class_list":["post-2752","knowledge2","type-knowledge2","status-publish","has-post-thumbnail","hentry","knowledge2_cat-biji"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - 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