{"id":2768,"date":"2024-05-28T16:30:08","date_gmt":"2024-05-28T08:30:08","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2768"},"modified":"2024-09-03T17:44:36","modified_gmt":"2024-09-03T09:44:36","slug":"opentrons-flex-2","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/opentrons-flex-2\/","title":{"rendered":"\u4f7f\u7528 Opentrons Flex\u2122 \u6838\u9178\u8403\u53d6\u5de5\u4f5c\u7ad9\u7d14\u5316\u5f9e\u7d30\u80de\u6216\u7d44\u7e54\u6a23\u672c\u4e2d\u63d0\u53d6\u57fa\u56e0\u7d44 DNA"},"content":{"rendered":"<!-- wp:paragraph -->\n<p><strong>\u61c9\u7528\u512a\u52e2<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u4f7f\u7528 Opentrons Flex\u2122 \u642d\u914dNucleoMag\u00ae Tissue \u8a66\u5291\u76d2\uff0c\u9032\u884c\u7d14\u5316\u6838\u9178\u7684\u512a\u9ede\uff1a<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph {\"align\":\"left\"} -->\n<p class=\"has-text-align-left\">1.\u65b9\u6cd5\u7d93\u904e\u9a57\u8b49\uff0c\u78ba\u4fdd\u5168\u81ea\u52d5\u63d0\u53d6\u7d14\u5316\u904e\u7a0b\u6e96\u78ba\u6027\u3002 2.\u7522\u91cf\u548c\u7d14\u5ea6\u91cd\u8907\u6027\u597d\uff0c\u7a69\u5b9a\u56de\u6536\u57fa\u56e0\u7d44 DNA\u3002 3.\u8655\u7406\u80fd\u529b\u5f37\uff0c\u53ef\u540c\u6642\u8655\u7406\u6700\u591a 96\u500b\u6a23\u54c1\u3002 4.\u53ef\u5b58\u53d6 Opentrons \u5354\u5b9a\u5eab\u6216\u900f\u904eMACHEREY NAGEL \u7684\u81ea\u52d5\u5316\u6280\u8853\u652f\u63f4automation-bio@mn-net.com\u4ee5\u53d6\u5f97\u66f4\u591a\u8a73\u7d30\u8cc7\u8a0a\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u5c0e\u8a00<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u8403\u53d6\u57fa\u56e0\u7d44 DNA \u662f\u751f\u547d\u79d1\u5b78\u548c\u61c9\u7528\u6e2c\u8a66\u5be6\u9a57\u5ba4\u4e2d\u5e38\u898b\u7684\u5be6\u9a57\u6d41\u7a0b\uff0c\u5c24\u5176\u5728\u57fa\u56e0\u5206\u578b\u6e2c\u8a66\u3001\u5b9a\u5e8f\u548c\u81e8\u5e8a\u7814\u7a76\u7b49\u4e0b\u6e38\u61c9\u7528\u4e2d\uff0c\u5c0d DNA \u7684\u54c1\u8cea\u548c\u7d14\u6de8\u5ea6\u8981\u6c42\u56b4\u683c\u3002        MACHEREY NAGEL \u7684 NucleoMag\u00ae Tissue \u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u57fa\u65bc\u78c1\u73e0\u7684\u5feb\u901f\u57fa\u56e0\u7d44 DNA \u7d14\u5316\u89e3\u6c7a\u65b9\u6848\u3002\u6b64\u8a66\u5291\u76d2\u8a2d\u8a08\u7cbe\u7c21\uff0c\u9069\u7528\u65bc 96 \u5b54\u677f\u6a21\u5f0f\uff0c\u53ef\u70ba PCR\u3001NGS \u6216\u5176\u4ed6\u9175\u7d20\u53cd\u61c9\u63d0\u4f9b\u9ad8\u54c1\u8cea\u3001\u9ad8\u7d14\u5ea6\u7684 DNA \u6a21\u677f\u3002        \u7d50\u5408 Opentrons Flex\u2122 \u6838\u9178\u8403\u53d6\u5de5\u4f5c\u7ad9\uff0c\u53ef\u9ad8\u6548\u7d14\u5316 HeLa \u7d30\u80de\u57fa\u56e0\u7d44DNA\u3002\u5de5\u4f5c\u7ad9\u914d\u5099Opentrons Flex\u2122 \u74b0\u5f62\u78c1\u529b\u67b6\u3001\u52a0\u71b1\u632f\u76ea\u6a21\u7d44\uff0c\u4ee5\u53ca5-1000 \u00b5L \u55ae\u901a\u9053\u548c8 \u901a\u9053\u79fb\u6db2\u5668\uff0c\u9084\u53ef\u9078\u64c796 \u901a\u9053\u79fb\u6db2\u5668\uff0c\u9069\u7528\u65bc\u5404\u7a2e\u6a23\u672c\u985e\u578b\u63d0\u53d6DNA\uff0c\u5305\u62ec\u5176\u4ed6\u771f\u6838\u7d30\u80de\u6216\u7d44\u7e54\u3002        Opentrons Protocol Library \u4e2d\u63d0\u4f9b\u4e86\u7d50\u5408 Opentrons Flex\u2122 \u6838\u9178\u8403\u53d6\u5de5\u4f5c\u7ad9\u8207 NucleoMag\u00ae Tissue \u8a66\u5291\u76d2\u4f7f\u7528\u7684\u5354\u8b70\uff0c\u53ef\u76f4\u63a5\u4e0b\u8f09\u4f7f\u7528\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u61c9\u7528\u8aaa\u660e NucleoMag\u00ae \u7d44\u7e54\u8207 Opentrons Flex<\/strong>\u2122<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>NucleoMag\u00ae Tissue \u8a66\u5291\u76d2\u7684\u539f\u7406\u662f\u57fa\u65bc\u6838\u9178\u5728\u7279\u5b9a\u7d50\u5408\u689d\u4ef6\u4e0b\u53ef\u9006\u5438\u9644\u5230 NucleoMag\u00aeB-beads \u4e0a\u3002\u5728\u8655\u7406\u9ad8\u9054 20 mg \u7684\u7d44\u7e54\u6a23\u672c\u6216 1\u00d710^6 \u500b \u7d30\u80de\u6642\uff0c\u5148\u52a0\u5165\u86cb\u767d\u9176 K \u548c\u88c2\u89e3\u7de9\u885d\u6db2T1\uff0c\u7136\u5f8c\u900f\u904e\u52a0\u5165\u7de9\u885d\u6db2 MB2 \u4fc3\u9032\u6838\u9178\u8207\u78c1\u73e0\u7684\u53ef\u9006\u7d50\u5408\u3002\u96a8\u5f8c\uff0c\u5728\u78c1\u73e0\u5438\u9644\u5f8c\uff0c\u4f7f\u7528\u6d17\u6ecc\u7de9\u885d\u6db2 MB3 \u548c MB4 \u9032\u884c\u6d17\u6ecc\uff0c\u4ee5\u53bb\u9664\u9e7d\u548c\u5176\u4ed6\u96dc\u8cea\u3002\u63a5\u8457\u9032\u884c\u4e7e\u71e5\u6b65\u9a5f\u6216\u9032\u884c MB5 \u6d17\u6ecc\uff0c\u6700\u5f8c\u4f7f\u7528\u6d17\u812b\u7de9\u885d\u6db2 MB6 \u5c07 DNA \u6d17\u812b\u4e0b\u4f86\u3002\u6240\u6709\u7684\u79fb\u6db2\u548c\u78c1\u73e0\u7d14\u5316\u6b65\u9a5f\u90fd\u7531 Opentrons Flex\u2122 \u5b8c\u6210\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u61c9\u7528\u8cc7\u6599<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2587,\"width\":\"566px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/001_20240513205647.jpg\" alt=\"\u5e94\u7528\u6570\u636e\" class=\"wp-image-2587\" style=\"width:566px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p><strong>\u5f9eHeLa\u7d30\u80de\u4e2d\u8403\u53d6DNA<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5229\u7528 Opentrons Flex\u2122 \u5de5\u4f5c\u7ad9\u642d\u914d NucleoMag\u00ae Tissue \u8a66\u5291\u76d2\uff0c\u6210\u529f\u5f9e2.4\u00d710^5 \u500b HeLa \u7d30\u80de\u4e2d\u8403\u53d6\u4e86\u9ad8\u7d14\u5ea6 DNA\u3002\u900f\u904e\u4f7f TapeStation \u5c0d\u7d14\u5316\u5f8c\u7684 DNA \u9032\u884c\u8a55\u4f30\uff0c\u5305\u62ec\u8a55\u4f30 DNA \u5b8c\u6574\u6027\uff08DIN\uff09\u548c\u7247\u6bb5\u5927\u5c0f\u5206\u4f48\uff08\u865b\u64ec\u51dd\u81a0\u6210\u50cf\uff09\uff0c\u7d50\u679c\u986f\u793a DNA \u7684\u5e73\u5747 DIN \u503c\u70ba9.0\u00b10.09\u3002\u9019\u8868\u660e Opentrons Flex\u2122 \u5de5\u4f5c\u7ad9\u8207 NucleoMag\u00ae Tissue\u8a66\u5291\u76d2\u7d50\u5408\u4f7f\u7528\uff0c\u80fd\u5920\u7a69\u5b9a\u5730\u5f9e HeLa \u7d30\u80de\u4e2d\u63d0\u53d6\u9ad8\u54c1\u8cea\u7684 DNA \u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u503c\u5f97\u4fe1\u8cf4\u7684 qPCR \u6aa2\u6e2c\u7d50\u679c<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u900f\u904e\u9032\u884c qPCR \u5206\u6790\uff08\u4ee5\u85cd\u8272\u689d\u8868\u793a\uff09\uff0c\u4f7f\u7528 PowerTrackTM SYBR GreenMaster Mix \u4f5c\u70ba\u8a66\u5291\uff0c\u5f15\u5b50\u7d93 IDT \u8a2d\u8a08\uff0c\u9776\u5411\u57fa\u56e0\u7d44\u4e2d 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