{"id":2811,"date":"2024-05-30T21:38:11","date_gmt":"2024-05-30T13:38:11","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2811"},"modified":"2024-09-03T12:00:12","modified_gmt":"2024-09-03T04:00:12","slug":"fully-automatic-pipetting","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/fully-automatic-pipetting\/","title":{"rendered":"\u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u8aaa\u660e\u624b\u518a"},"content":{"rendered":"<!-- wp:image {\"id\":2695,\"width\":\"350px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\",\"align\":\"center\"} -->\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/image-23.png\" alt=\"\u5168\u81ea\u52a8\u79fb\u6db2\u5de5\u4f5c\u7ad9\" class=\"wp-image-2695\" style=\"width:350px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p><strong>\u524d\u8a00<\/strong>\u6b61\u8fce\u95b1\u8b80 Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u8aaa\u660e\u624b\u518a\u3002\u672c\u624b\u518a\u70ba\u60a8\u65e5\u5e38\u8a2d\u5b9a\u548c\u4f7f\u7528 Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u63d0\u4f9b\u5168\u9762\u6307\u5c0e\uff0c\u91cd\u9ede\u5728\u65bc\u5be6\u9a57\u5ba4\u74b0\u5883\u4e2dFlex\u65e5\u5e38\u4f7f\u7528\u8005\u6700\u70ba\u95dc\u5207\u7684\u8a31\u591a\u4e3b\u984c\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u624b\u518a\u7d50\u69cb<\/strong>Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u662f\u4e00\u5957\u8907\u96dc\u7cfb\u7d71\uff0c\u60a8\u53ef\u4ee5\u5f9e\u4e0d\u540c\u9014\u5f91\u4e86\u89e3\u9019\u5957\u7cfb\u7d71\uff0c\u5fc5\u8981\u6642\u4e5f\u53ef\u4ee5\u76f4\u63a5\u8df3\u5230\u60a8\u611f\u8208\u8da3\u7684\u7ae0\u7bc0\u95b1\u8b80\uff01\u4f8b\u5982\uff0c\u5982\u679c\u60a8\u5be6\u9a57\u5ba4\u88e1\u5df2\u7d93\u59a5\u5584\u5b89\u88dd\u4e86 Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\uff0c\u90a3\u9ebc\u60a8\u53ef\u4ee5\u8df3\u904e\u5b89\u88dd\u8207\u79fb\u52d5\u7ae0\u7bc0\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5982\u679c\u60a8\u559c\u6b61\u5f15\u5c0e\u5f0f\u95b1\u8b80\uff0c\u672c\u624b\u518a\u505a\u4e86\u7cbe\u5fc3\u7684\u7d50\u69cb\u8a2d\u8a08\uff0c\u60a8\u53ef\u4ee5\u5f9e\u958b\u982d\u5230\u7d50\u5c3e\u9032\u884c\u7cfb\u7d71\u95b1\u8b80\u3002 \u25aa \u95dc\u65bc Flex\u3002\u7b2c\u4e00\u7ae0\u7c21\u4ecb Flex \u7684\u4e3b\u8981\u7279\u6027\uff0c\u4ee5\u53ca\u91cd\u8981\u7684\u5b89\u5168\u8cc7\u8a0a\u548c\u76e3\u7ba1\u8cc7\u8a0a\u3002 \u25aa \u958b\u7bb1 Flex\u3002\u5982\u679c\u60a8\u9700\u8981\u5b89\u88dd Flex\uff0c\u8acb\u200b\u200b\u4f9d\u7167\u7b2c\u4e8c\u7ae0\u5b89\u88dd\u8207\u79fb\u52d5\u4e2d\u7684\u8a73\u7d30\u8aaa\u660e\u5b89\u88dd\uff0c\u7136\u5f8c\u5f9e\u7b2c\u4e09\u7ae0\u7cfb\u7d71\u8aaa\u660e\u4e2d\u4e86\u89e3 Flex \u7684\u57fa\u672c\u5143\u4ef6\u3002 \u25aa \u8a2d\u7f6e\u7532\u677f\u3002\u914d\u7f6e\u7532\u677f\uff0c\u555f\u7528 Flex \u61c9\u7528\u7a0b\u5f0f\u3002\u7b2c\u56db\u7ae0\u6a21\u7d44\u4ecb\u7d39\u53ef\u4ee5\u5d4c\u5165\u6216\u642d\u8f09\u5728\u7532\u677f\u4e0a\u5de5\u4f5c\u7684 Opentrons \u5916\u570d\u6a21\u7d44\u6216\u786c\u9ad4\u3002\u7b2c\u4e94\u7ae0\u5be6\u9a57\u5ba4\u8aaa\u660e\u5982\u4f55\u4f7f\u7528\u6a5f\u5668\u79fb\u6db2\u3002 \u25aa \u904b\u884c\u5354\u8b70\u3002 Flex \u7684\u4e3b\u8981\u7528\u9014\u662f\u904b\u884c\u6a19\u6e96\u5316\u79d1\u5b78\u7a0b\u5e8f\uff0c\u5373\u5354\u8b70\u3002\u7b2c\u516d\u7ae0\u5354\u8b70\u958b\u767c\u4ecb\u7d39\u5982\u4f55\u53d6\u5f97\u73fe\u6210\u7684\u5354\u8b70\u6216\u81ea\u884c\u8a2d\u8a08\u5354\u8b70\u3002\u7b2c\u4e03\u7ae0\u8edf\u9ad4\u8207\u64cd\u4f5c\u4ecb\u7d39\u5982\u4f55\u904b\u4f5c\u5354\u8b70\uff0c\u5982\u4f55\u57f7\u884c\u5176\u5b83\u4efb\u52d9\u548c\u81ea\u8a02\u6a5f\u5668\u8a2d\u5b9a\u3002 \u25aa Flex \u7dad\u8b77\u3002\u4f9d\u7167\u7b2c\u516b\u7ae0\u7dad\u8b77\u8207\u670d\u52d9\u5efa\u8b70\uff0c\u53ca\u6642\u6e05\u6f54 Flex\uff0c\u4fdd\u6301\u6700\u4f73\u904b\u4f5c\u72c0\u614b\uff1b\u6216\u7533\u8acbOpentrons \u670d\u52d9\u9805\u76ee\uff0c\u8b93\u6211\u5011\u5e6b\u60a8\u7dad\u8b77\u3002 \u25aa \u66f4\u591a\u5176\u4ed6\u8cc7\u8a0a\u3002\u9084\u60f3\u4e86\u89e3\u5b83\uff1f\u53ef\u4ee5\u67e5\u770b\u9644\u9304\u3002 \u25aa \u9644\u9304 A \u8853\u8a9e\u8868\u5b9a\u7fa9\u4e86 Flex \u76f8\u95dc\u8853\u8a9e\u3002 \u25aa \u9644\u9304 B \u5176\u5b83\u8cc7\u6599\u4ecb\u7d39\u66f4\u591a\u6709\u95dc Opentrons \u7522\u54c1\u548c\u65b9\u4fbf\u7de8\u5beb Flex \u63a7\u88fd\u7a0b\u5f0f\u78bc\u7684\u8cc7\u6e90\u3002 \u25aa \u9644\u9304 C \u958b\u6e90\u8edf\u9ad4\u4ecb\u7d39\u4e86\u4f9b\u958b\u767c\u8005\u548c\u975e\u958b\u767c\u8005\u4f7f\u7528\u7684 GitHub Opentrons \u8edf\u9ad4\u8cc7\u6e90\u3002 \u25aa \u9644\u9304 D \u652f\u63f4\u8207\u806f\u7d61\u65b9\u5f0f\u5217\u660e\u4e86 Opentrons \u806f\u7d61\u65b9\u5f0f\uff0c\u65b9\u4fbf\u60a8\u5411\u6211\u5011\u5c0b\u6c42\u5176\u5b83\u5354\u52a9\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u6ce8\u610f\u8207\u8b66\u544a<\/strong>\u672c\u624b\u518a\u4e2d\u6709\u4e00\u4e9b\u5c08\u9580\u7684\u6ce8\u610f\u548c\u8b66\u544a\u6a19\u793a\u3002 \u201c\u6ce8\u610f\u201d\uff0c\u70ba\u60a8\u63d0\u4f9b\u4e00\u4e9b\u65e5\u5e38\u4f7f\u7528\u904e\u7a0b\u4e2d\u53ef\u80fd\u4e0d\u6703\u6ce8\u610f\u5230\u7684\u5be6\u7528\u8cc7\u8a0a\u3002 \u201c\u8b66\u544a\u201d\uff0c\u63d0\u9192\u60a8\u8981\u7279\u5225\u91cd\u8996\uff0c\u5b83\u7684\u51fa\u73fe\u610f\u5473\u8457\u53ef\u80fd\u6703\u5f15\u767c\u4eba\u8eab\u50b7\u5bb3\u3001\u8a2d\u5099\u640d\u58de\u3001\u6a23\u54c1\u6216\u8a66\u5291\u640d\u8017\u6216\u6c61\u67d3\u3001\u8cc7\u6599\u907a\u5931\u6216\u5176\u4ed6\u50b7\u5bb3\u98a8\u96aa\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u7b2c\u4e00\u7ae0\u7c21\u4ecb<\/strong>\u672c\u7ae0\u4ecb\u7d39Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u751f\u614b\u7cfb\u7d71\uff0c\u5305\u62ec\u6574\u9ad4\u7cfb\u7d71\u8a2d\u8a08\u548c\u53ef\u7528\u7684\u5de5\u4f5c\u7ad9\u914d\u7f6e\uff0c\u4ee5\u53ca\u60a8\u5b89\u88ddOpentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u6642\uff0c\u8981\u4e86\u89e3\u7684\u91cd\u8981\u5408\u898f\u8207\u5b89\u5168\u8cc7\u8a0a\u3002\u95dc\u65bc Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\u7684\u66f4\u591a\u8a73\u7d30\u4fe1\u606f\uff0c\u8acb\u53c3\u8003\u7b2c\u4e09\u7ae0\u7cfb\u7d71\u63cf\u8ff0\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>1.1 \u6b61\u8fce\u4f7f\u7528 Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9<\/strong>Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\uff0c\u662f\u4e00\u6b3e\u9069\u7528\u65bc\u9ad8\u901a\u91cf\u8907\u96dc\u5de5\u4f5c\u6d41\u7a0b\u7684\u79fb\u6db2\u6a5f\u5668\u3002 Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u662f\u6a21\u7d44\u5316\u7cfb\u7d71\u7684\u57fa\u790e\u5e73\u53f0\uff0c\u60a8\u53ef\u4ee5\u6839\u64da\u9700\u8981\u5728\u4e0a\u9762\u81ea\u884c\u642d\u8f09\u79fb\u6db2\u5668\u3001\u8f49\u677f\u6293\u624b\u3001\u7532\u677f\u4e0a\u6a21\u7d44\u548c\u5be6\u9a57\u5668\u76bf\u7b49\u6a21\u7d44\u7cfb\u7d71\u3002\u60a8\u53ef\u4ee5\u900f\u904e Flex \u89f8\u63a7\u87a2\u5e55\u76f4\u63a5\u64cd\u4f5c\u6a5f\u5668\uff0c\u4e5f\u53ef\u4ee5\u900f\u904eOpentrons App \u6216\u958b\u6e90 API \u5728\u5be6\u9a57\u5ba4\u5176\u5b83\u5730\u65b9\u9060\u7aef\u64cd\u4f5c\u6a5f\u5668\u3002 Opentrons Flex \u5168\u81ea\u52d5\u79fb\u6db2\u5de5\u4f5c\u7ad9\uff0c\u63d0\u4f9b\u60a8\u555f\u52d5\u81ea\u52d5\u5316\u5e38\u898f\u5be6\u9a57\u5ba4\u5de5\u4f5c\u6240\u9700\u7684\u6240\u6709\u8a2d\u5099\uff0c\u5305\u62ec\u6a5f\u5668\u3001\u786c\u9ad4\u548c\u5be6\u9a57\u5ba4\u5668\u76bf\u3002\u95dc\u65bc\u5176\u4ed6\u61c9\u7528\uff0cOpentrons Flex \u5b8c\u5168\u57fa\u65bc\u958b\u6e90\u8edf\u9ad4\u548c\u97cc\u9ad4\u904b\u884c\uff0c\u7121\u8a66\u5291\u548c\u5be6\u9a57\u5ba4\u5668\u76bf\u9650\u5236\uff0c\u60a8\u53ef\u4ee5\u81ea\u4e3b\u8a2d\u8a08\u548c\u904b\u884c\u5354\u8b70\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>Flex \u65b0\u529f\u80fd<\/strong>Opentrons Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u662f Opentrons \u7cfb\u5217\u79fb\u6db2\u6a5f\u4e4b\u4e00\u3002 Opentrons Flex \u79fb\u6db2\u5de5\u4f5c\u7ad9\u4f7f\u7528\u8005\u53ef\u80fd\u4e5f\u77e5\u9053 Opentrons OT-2 \u79fb\u6db2\u5de5\u4f5c\u7ad9\u3002\u5728\u8a31\u591a\u95dc\u9375\u65b9\u9762\uff0cFlex \u90fd\u6bd4 OT-2 \u66f4\u52dd\u4e00\u7c4c\uff0c\u901a\u91cf\u66f4\u5927\uff0c\u81ea\u52d5\u5316\u7a0b\u5ea6\u66f4\u9ad8\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":2696,\"width\":\"433px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/image-24.png\" alt=\"\u7279\u6027\u63cf\u8ff0\" class=\"wp-image-2696\" style=\"width:433px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":2697,\"width\":\"433px\",\"height\":\"auto\",\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full is-resized\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/05\/image-25.png\" alt=\"\u7279\u6027\u63cf\u8ff0\" class=\"wp-image-2697\" style=\"width:433px;height:auto\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p><strong>Opentrons \u7db2\u7ad9\u63d0\u4f9b\u8a73\u7d30\u7684\u6a5f\u5668\u6280\u8853\u53c3\u6578\u6bd4\u8f03\u3002 <\/strong>Flex \u548c OT-2 \u6a5f\u5668\u90fd\u57fa\u65bc\u958b\u6e90\u8edf\u9ad4\u904b\u884c\uff0cOpentrons App \u80fd\u540c\u6642\u63a7\u5236\u9019\u5169\u7a2e\u6a5f\u5668\u3002\u96d6\u7136 OT-2\u5354\u5b9a\u7121\u6cd5\u76f4\u63a5\u5728 Flex \u4e0a\u904b\u884c\uff0c\u4f46\u6539\u7de8\u5f88\u7c21\u55ae\uff08\u53c3\u8003\u5354\u5b9a\u958b\u767c 6.4 OT-2 \u5354\u5b9a\u90e8\u5206\uff09\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>Flex \u5de5\u4f5c\u7ad9<\/strong>Opentrons Flex \u5de5\u4f5c\u7ad9\u7531\u6a5f\u5668\u3001\u914d\u4ef6\u3001\u79fb\u6db2\u5668\u548c\u8f49\u677f\u6293\u624b\u3001\u6a21\u7d44\u3001\u4ee5\u53ca\u5be6\u73fe\u7279\u5b9a\u81ea\u52d5\u5316\u61c9\u7528\u6240\u9700\u7684\u5668\u76bf\u7d44\u6210\u3002\u5de5\u4f5c\u7ad9\u6240\u6709\u7d44\u4ef6\u90fd\u662f\u6a21\u7d44\u5f0f\u7d44\u4ef6\u3002\u5982\u679c\u60a8\u60f3\u8981\u8b8a\u66f4\u7528\u9014\uff0c\u53ef\u4ee5\u65b0\u589e\u6216\u66f4\u63db\u5176\u5b83 Flex \u786c\u9ad4\u548c\u76f8\u5bb9\u8017\u6750\u3002 <strong>NGS \u5de5\u4f5c\u7ad9<\/strong>Opentrons Flex NGS \u5de5\u4f5c\u7ad9\u652f\u63f4\u81ea\u52d5\u88fd\u5099 NGS \u6587\u5eab\uff0c\u4f7f\u7528\u4e3b\u6d41\u8a66\u5291\u7cfb\u7d71\uff0c\u81ea\u52d5\u57f7\u884c\u5b9a\u5e8f\u524d\u5de5\u4f5c\u6d41\u7a0b\uff0c\u4f8b\u5982\u7247\u6bb5\u5316\u3001\u6a19\u9776\u6587\u5eab\u88fd\u5099\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u9664\u4e86Flex \u6a5f\u5668\uff0cNGS \u5de5\u4f5c\u7ad9\u9084\u5305\u62ec\uff1a\u25fe \u8f49\u677f\u6293\u624b\u25fe \u5169\u500b8 \u901a\u9053\u79fb\u6db2\u5668\uff081-50\u03bcL \u548c5-1000\u03bcL\uff09\u25fe \u74b0\u5f62\u78c1\u529b\u67b6\u25fe \u6eab\u63a7\u6a21\u7d44\u25fe \u71b1\u5faa\u74b0\u6a21\u7d44\u25fe \u914d\u5957\u5be6\u9a57\u5ba4\u5668\u76bf\uff08\u6ffe\u82af\u5438\u982d\u3001\u5fae\u91cf\u96e2\u5fc3\u7ba1\u3001\u5132\u6db2\u69fd\u548cPCR \u5b54\u677f\uff09PCR \u5de5\u4f5c\u7ad9Opentrons Flex PCR\u5de5\u4f5c\u7ad9\uff0c\u80fd\u540c\u6642\u70ba\u6700\u591a 96 \u4efd\u6a23\u672c\u81ea\u52d5\u9032\u884c PCR 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